Restriction map for
pFN21AB5139
(Circular) (Six-base) MAPSORT of: pFN21AB5139.seq Check: 367 from: 1 to: 6406
pFN21AB5139 6406 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3224 3224
Size: 6406
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2700 3749 1937
Size: 763 1049 4594
Fragments arranged by size:
4594 1049 763
Acc65I G'GTAC_C
Cuts at: 3043 3727 3043
Size: 684 5722
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3475 3559 4469 5853 6121 1632
Size: 1843 84 910 1384 268 1917
Fragments arranged by size:
1917 1843 1384 910 268 84
AfeI AGC'GCT
Cuts at: 2735 2819 3167 2735
Size: 84 348 5974
Fragments arranged by size:
5974 348 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 5305 6282 1203
Size: 4102 977 1327
Fragments arranged by size:
4102 1327 977
AgeI A'CCGG_T
Cuts at: 5237 5237
Size: 6406
AhdI GACnn_n'nnGTC
Cuts at: 5135 5135
Size: 6406
AleI CACnn'nnGTG
Cuts at: 1166 2135 2364 1166
Size: 969 229 5208
Fragments arranged by size:
5208 969 229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6406
AlwNI CAG_nnn'CTG
Cuts at: 2095 4149 5721 2095
Size: 2054 1572 2780
Fragments arranged by size:
2780 2054 1572
ApaI G_GGCC'C
Cuts at: 2030 2073 2173 2416 2646 3082 3181 2030
Size: 43 100 243 230 436 99 5255
Fragments arranged by size:
5255 436 243 230 100 99 43
ApaLI G'TGCA_C
Cuts at: 2597 2774 3275 4463 5619 2597
Size: 177 501 1188 1156 3384
Fragments arranged by size:
3384 1188 1156 501 177
ApoI r'AATT_y
Cuts at: 1493 3715 3941 4093 6202 1493
Size: 2222 226 152 2109 1697
Fragments arranged by size:
2222 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 2804 2983 3731 1870
Size: 73 861 179 748 4545
Fragments arranged by size:
4545 861 748 179 73
BamHI G'GATC_C
Cuts at: 3736 4103 3736
Size: 367 6039
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2238 2502 3043 3626
Size: 325 217 627 363 88 264 541 583
Cuts at: 3626 3727 5182 618
Size: 101 1455 1842
Fragments arranged by size:
1842 1455 627 583 541 363 325 264
217 101 88
BanII G_rGCy'C
Cuts at: 1391 2030 2073 2173 2416 2491 2646 2993 3033
Size: 639 43 100 243 75 155 347 40
Cuts at: 3033 3082 3181 3725 1391
Size: 49 99 544 4072
Fragments arranged by size:
4072 639 544 347 243 155 100 99
75 49 43 40
BbeI G_GCGC'C
Cuts at: 3630 3630
Size: 6406
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3254 928
Size: 971 1355 4080
Fragments arranged by size:
4080 1355 971
BbvCI CC'TCA_GC
Cuts at: 2117 2607 2117
Size: 490 5916
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4597 4631 1903
Size: 34 50 34 2576 34 3678
Fragments arranged by size:
3678 2576 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4298 5508 4298
Size: 1210 5196
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2522 5017 136
Size: 107 122 71 631 605 850 2495 1525
Fragments arranged by size:
2495 1525 850 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6401 6401
Size: 6406
BlpI GC'TnA_GC
Cuts at: 3823 3823
Size: 6406
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2073 2155 2173 2185 2416 2601 2646
Size: 240 43 82 18 12 231 185 45
Cuts at: 2646 2778 2804 3082 3181 3279 4467 5623 1790
Size: 132 26 278 99 98 1188 1156 2573
Fragments arranged by size:
2573 1188 1156 278 240 231 185 132
99 98 82 45 43 26 18 12
BmgBI CAC'GTC
Cuts at: 1318 2133 1318
Size: 815 5591
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2137 2560 4137
Size: 416 53 83 399 24 720 423 1577
Cuts at: 4137 5095 442
Size: 958 1753
Fragments arranged by size:
1753 1577 958 720 423 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2636 3182 5066 1239
Size: 1397 546 1884 2579
Fragments arranged by size:
2579 1884 1397 546
Bpu10I CC'TnA_GC
Cuts at: 2117 2607 2117
Size: 490 5916
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4531 5396 5694 4531
Size: 865 298 5243
Fragments arranged by size:
5243 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5069 882
Size: 617 3570 2219
Fragments arranged by size:
3570 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2602 4197 493
Size: 711 1398 1595 2702
Fragments arranged by size:
2702 1595 1398 711
BsaBI GATnn'nnATC
Cuts at: 6400 6400
Size: 6406
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3627 4213 4595 5230
Size: 53 83 186 936 2094 586 382 635
Cuts at: 5230 275
Size: 1451
Fragments arranged by size:
2094 1451 936 635 586 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4839 5237 5511 5658 6069 4839
Size: 398 274 147 411 5176
Fragments arranged by size:
5176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2682 2712 1178
Size: 30 165 30 1279 30 4872
Fragments arranged by size:
4872 1279 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2633 3320 3683 1186
Size: 1447 687 363 3909
Fragments arranged by size:
3909 1447 687 363
BseYI C'CCAG_C
Cuts at: 2049 2208 2385 2994 5609 6105 2049
Size: 159 177 609 2615 496 2350
Fragments arranged by size:
2615 2350 609 496 177 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2294 1163
Size: 573 558 5275
Fragments arranged by size:
5275 573 558
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2704 2899 3027 3108 3316 3373
Size: 275 186 703 195 128 81 208 57
Cuts at: 3373 3568 3856 4617 5645 1540
Size: 195 288 761 1028 2301
Fragments arranged by size:
2301 1028 761 703 288 275 208 195
195 186 128 81 57
BsiHKAI G_wGCw'C
Cuts at: 2491 2601 2778 3093 3279 4467 4552 5623 2491
Size: 110 177 315 186 1188 85 1071 3274
Fragments arranged by size:
3274 1188 1071 315 186 177 110 85
BsmI GAATG_Cn'
Cuts at: 3917 4010 3917
Size: 93 6313
BsmBI CGTCTCn'nnnn_
Cuts at: 2452 2452
Size: 6406
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2073 2155 2173 2185 2416 2491
Size: 399 240 43 82 18 12 231 75
Cuts at: 2491 2601 2646 2778 2804 2993 3033 3082 3093
Size: 110 45 132 26 189 40 49 11
Cuts at: 3093 3181 3279 3725 4467 4552 5623 1391
Size: 88 98 446 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 446 399 240 231 189
132 110 98 88 85 82 75 49
45 43 40 26 18 12 11
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 4295 6025 4295
Size: 1730 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2931 3224 3761 844
Size: 2087 293 537 3489
Fragments arranged by size:
3489 2087 537 293
BsrBI CCG'CTC
Cuts at: 1394 1823 2788 3205 3289 3541 4293 1394
Size: 429 965 417 84 252 752 3507
Fragments arranged by size:
3507 965 752 429 417 252 84
BsrDI GCAATG_nn'
Cuts at: 65 1248 4901 5075 65
Size: 1183 3653 174 1396
Fragments arranged by size:
3653 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2235 2850 2864 3005 3055 3435 3449
Size: 110 425 615 14 141 50 380 14
Cuts at: 3449 3519 5050 5237 1700
Size: 70 1531 187 2869
Fragments arranged by size:
2869 1531 615 425 380 187 141 110
70 50 14 14
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 4466 5478 4466
Size: 1012 5394
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2892 794
Size: 2098 4308
BstBI TT'CG_AA
Cuts at: 5210 5210
Size: 6406
BstEII G'GTnAC_C
Cuts at: 1184 2668 1184
Size: 1484 4922
BstXI CCAn_nnnn'nTGG
Cuts at: 2044 2366 2638 2044
Size: 322 272 5812
Fragments arranged by size:
5812 322 272
BstYI r'GATC_y
Cuts at: 1972 3736 4103 4488 4505 5957 6043 6055 6401
Size: 1764 367 385 17 1452 86 12 346
Cuts at: 6401 1972
Size: 1977
Fragments arranged by size:
1977 1764 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2037 2110 2664 3021 513
Size: 552 972 73 554 357 3898
Fragments arranged by size:
3898 972 554 552 357 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2159 2422 487
Size: 683 56 933 263 4471
Fragments arranged by size:
4471 933 683 263 56
BtsI GCAGTG_nn'
Cuts at: 2306 3922 4716 4736 2306
Size: 1616 794 20 3976
Fragments arranged by size:
3976 1616 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6371
DraI TTT'AAA
Cuts at: 3710 3710
Size: 6406
DraIII CAC_nnn'GTG
Cuts at: 3259 3259
Size: 6406
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 809
Size: 507 4097 1802
Fragments arranged by size:
4097 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2275 2551
Size: 54 1425 50 275 18 204 241 276
Cuts at: 2551 2896 3024 3313 3649 3853 4742 6371 8
Size: 345 128 289 336 204 889 1629 43
Fragments arranged by size:
1629 1425 889 345 336 289 276 275
241 204 204 128 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2896 3024 3313 3853 1537
Size: 275 1084 128 289 540 4090
Fragments arranged by size:
4090 1084 540 289 275 128
EarI CTCTTCn'nnn_
Cuts at: 1353 4336 1353
Size: 2983 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3398 4993 5367 5513 387
Size: 1064 138 1809 1595 374 146 1280
Fragments arranged by size:
1809 1595 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2489 2489
Size: 6406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2636 3182 3475 3559 4469 5066 5853
Size: 393 1004 546 293 84 910 597 787
Cuts at: 5853 6121 1239
Size: 268 1524
Fragments arranged by size:
1524 1004 910 787 597 546 393 293
268 84
EcoNI CCTnn'n_nnAGG
Cuts at: 2099 2618 2099
Size: 519 5887
EcoO109I rG'GnC_Cy
Cuts at: 2026 2027 2070 2169 2611 2642 2643 3078 3079
Size: 1 43 99 442 31 1 435 1
Cuts at: 3079 3100 2026
Size: 21 5332
Fragments arranged by size:
5332 442 435 99 43 31 21 1
1 1
EcoRI G'AATT_C
Cuts at: 3715 4093 3715
Size: 378 6028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 6406
FspI TGC'GCA
Cuts at: 4912 4912
Size: 6406
HaeII r_GCGC'y
Cuts at: 1678 2737 2821 3169 3630 5553 1678
Size: 1059 84 348 461 1923 2531
Fragments arranged by size:
2531 1923 1059 461 348 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2682 2714 5047 5079 5121
Size: 32 135 32 1110 32 2333 32 42
Cuts at: 5121 5153 1373
Size: 32 2626
Fragments arranged by size:
2626 2333 1110 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2701 3750 3996 669
Size: 988 281 763 1049 246 3079
Fragments arranged by size:
3079 1049 988 763 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6406
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KasI G'GCGC_C
Cuts at: 3626 3626
Size: 6406
KpnI G_GTAC'C
Cuts at: 3047 3731 3047
Size: 684 5722
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3528 3612 5520 5704 1305
Size: 2223 84 1908 184 2007
Fragments arranged by size:
2223 2007 1908 184 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2036 3651 10
Size: 54 1425 343 204 1615 2765
Fragments arranged by size:
2765 1615 1425 343 204 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 2178 2364 4364 4723 4882 518
Size: 648 969 43 186 2000 359 159 2042
Fragments arranged by size:
2042 2000 969 648 359 186 159 43
MspA1I CmG'CkG
Cuts at: 1606 2112 2423 2666 2998 3023 3132 3187 3822
Size: 506 311 243 332 25 109 55 635
Cuts at: 3822 4499 5647 5892 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 635 506 332 311 245
243 109 55 25
NaeI GCC'GGC
Cuts at: 3007 3007
Size: 6406
NarI GG'CG_CC
Cuts at: 3627 3627
Size: 6406
NcoI C'CATG_G
Cuts at: 513 1065 2037 513
Size: 552 972 4882
Fragments arranged by size:
4882 972 552
NdeI CA'TA_TG
Cuts at: 387 2537 387
Size: 2150 4256
NgoMIV G'CCGG_C
Cuts at: 3005 3005
Size: 6406
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 2896 3313 3853 2896
Size: 417 540 5449
Fragments arranged by size:
5449 540 417
NspI r_CATG'y
Cuts at: 1132 1229 3764 5309 1132
Size: 97 2535 1545 2229
Fragments arranged by size:
2535 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2095 2652 3328 1348
Size: 747 557 676 4426
Fragments arranged by size:
4426 747 676 557
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2095 1108
Size: 987 5419
PfoI T'CCnGG_A
Cuts at: 3618 3618
Size: 6406
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 5988 6020 4475
Size: 32 1481 32 4861
Fragments arranged by size:
4861 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3100 3100
Size: 6406
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6406
PsiI TTA'TAA
Cuts at: 3976 3976
Size: 6406
PspOMI G'GGCC_C
Cuts at: 2026 2069 2169 2412 2642 3078 3177 2026
Size: 43 100 243 230 436 99 5255
Fragments arranged by size:
5255 436 243 230 100 99 43
PstI C_TGCA'G
Cuts at: 830 3758 830
Size: 2928 3478
PvuI CG_AT'CG
Cuts at: 2001 3108 3373 2001
Size: 1107 265 5034
Fragments arranged by size:
5034 1107 265
PvuII CAG'CTG
Cuts at: 2423 2998 3132 2423
Size: 575 134 5697
Fragments arranged by size:
5697 575 134
SacI G_AGCT'C
Cuts at: 2491 2491
Size: 6406
SacII CC_GC'GG
Cuts at: 2113 2667 3024 2113
Size: 554 357 5495
Fragments arranged by size:
5495 554 357
SalI G'TCGA_C
Cuts at: 1936 2699 3748 1936
Size: 763 1049 4594
Fragments arranged by size:
4594 1049 763
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 4654 6366 4654
Size: 1712 4694
SexAI A'CCwGG_T
Cuts at: 3255 3507 3255
Size: 252 6154
SfcI C'TryA_G
Cuts at: 826 1045 3127 3754 4889 5570 5761 826
Size: 219 2082 627 1135 681 191 1471
Fragments arranged by size:
2082 1471 1135 681 627 219 191
SfoI GGC'GCC
Cuts at: 3628 3628
Size: 6406
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 2235 2850 2864 3435 3449 3519 1700
Size: 535 615 14 571 14 70 4587
Fragments arranged by size:
4587 615 571 535 70 14 14
SmaI CCC'GGG
Cuts at: 1872 2985 3733 1872
Size: 1113 748 4545
Fragments arranged by size:
4545 1113 748
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4510 5411 5673 820
Size: 197 926 2567 901 262 1553
Fragments arranged by size:
2567 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 3404 1517
Size: 1887 4519
StyI C'CwwG_G
Cuts at: 513 1065 2037 2631 3845 513
Size: 552 972 594 1214 3074
Fragments arranged by size:
3074 1214 972 594 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2200 2377 3108 3614 4433 4450 4603
Size: 1255 620 177 731 506 819 17 153
Cuts at: 4603 4788 5233 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 819 731 620 506 445 185
177 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4652 6364 96
Size: 274 80 33 51 1443 2675 1712 138
Fragments arranged by size:
2675 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2936 2968 6366 6398 2936
Size: 32 3398 32 2944
Fragments arranged by size:
3398 2944 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6406
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2341 2341
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 2983 3731 1870
Size: 1113 748 4545
Fragments arranged by size:
4545 1113 748
XmnI GAAnn'nnTTC
Cuts at: 3236 4535 3236
Size: 1299 5107
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
Bsu36I EcoRV FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI