Restriction map for
pFN21AB5138
(Circular) (Six-base) MAPSORT of: pFN21AB5138.seq Check: 1182 from: 1 to: 7090
pFN21AB5138 7090 bp
With 182 enzymes: SgfI *
October 20, 2009 10:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4900 278
Size: 53 83 186 936 3364 2468
Fragments arranged by size:
3364 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4433 1937
Size: 2496 4594
Acc65I G'GTAC_C
Cuts at: 4411 4411
Size: 7090
AclI AA'CG_TT
Cuts at: 1563 5217 5590 1563
Size: 3654 373 3063
Fragments arranged by size:
3654 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2603 4238 4295 5153 6537 6805 1632
Size: 971 1635 57 858 1384 268 1917
Fragments arranged by size:
1917 1635 1384 971 858 268 57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6893
AflIII A'CryG_T
Cuts at: 1203 3187 5989 6966 1203
Size: 1984 2802 977 1327
Fragments arranged by size:
2802 1984 1327 977
AgeI A'CCGG_T
Cuts at: 5921 5921
Size: 7090
AhdI GACnn_n'nnGTC
Cuts at: 5819 5819
Size: 7090
AleI CACnn'nnGTG
Cuts at: 1166 2567 3113 1166
Size: 1401 546 5143
Fragments arranged by size:
5143 1401 546
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3740 1184
Size: 2556 4534
AlwNI CAG_nnn'CTG
Cuts at: 2965 2983 4132 4833 6405 2965
Size: 18 1149 701 1572 3650
Fragments arranged by size:
3650 1572 1149 701 18
ApaLI G'TGCA_C
Cuts at: 5147 6303 5147
Size: 1156 5934
ApoI r'AATT_y
Cuts at: 1493 2294 2505 2708 3295 4050 4399 4625 4777
Size: 801 211 203 587 755 349 226 152
Cuts at: 4777 6886 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 801 755 587 349 226 211
203 152
AseI AT'TA_AT
Cuts at: 160 1032 3486 4371 5644 160
Size: 872 2454 885 1273 1606
Fragments arranged by size:
2454 1606 1273 885 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7090
AvaI C'yCGr_G
Cuts at: 1870 1943 2182 4415 1870
Size: 73 239 2233 4545
Fragments arranged by size:
4545 2233 239 73
BamHI G'GATC_C
Cuts at: 4420 4787 4420
Size: 367 6723
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2792 3922 4411 5866 618
Size: 325 217 627 1005 1130 489 1455 1842
Fragments arranged by size:
1842 1455 1130 1005 627 489 325 217
BanII G_rGCy'C
Cuts at: 1391 2120 2925 3788 4409 1391
Size: 729 805 863 621 4072
Fragments arranged by size:
4072 863 805 729 621
BbeI G_GCGC'C
Cuts at: 2796 2796
Size: 7090
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2541 2744 3111 3668 4037 928
Size: 971 642 203 367 557 369 3981
Fragments arranged by size:
3981 971 642 557 369 367 203
BbvCI CC'TCA_GC
Cuts at: 3681 3876 3681
Size: 195 6895
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5281 5315 1903
Size: 34 3344 34 3678
Fragments arranged by size:
3678 3344 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4982 6192 4982
Size: 1210 5880
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7090
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5701 136
Size: 107 122 71 631 605 4029 1525
Fragments arranged by size:
4029 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3068 7085 3068
Size: 4017 3073
BlpI GC'TnA_GC
Cuts at: 2976 4507 2976
Size: 1531 5559
Bme1580I G_kGCm'C
Cuts at: 1790 2633 5151 6307 1790
Size: 843 2518 1156 2573
Fragments arranged by size:
2573 2518 1156 843
BmgBI CAC'GTC
Cuts at: 1318 4096 1318
Size: 2778 4312
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3256 4821 5779
Size: 416 53 83 399 24 1839 1565 958
Cuts at: 5779 442
Size: 1753
Fragments arranged by size:
1839 1753 1565 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7090
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2595 3667 3807 2595
Size: 1072 140 5878
Fragments arranged by size:
5878 1072 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 3606 3984 4061 5750 1239
Size: 884 1483 378 77 1689 2579
Fragments arranged by size:
2579 1689 1483 884 378 77
Bpu10I CC'TnA_GC
Cuts at: 3681 3876 3681
Size: 195 6895
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3525 4184 5215 6080 6378 3525
Size: 659 1031 865 298 4237
Fragments arranged by size:
4237 1031 865 659 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3318 5753 882
Size: 617 1819 2435 2219
Fragments arranged by size:
2435 2219 1819 617
BsaAI yAC'GTr
Cuts at: 493 1204 2437 3188 4881 493
Size: 711 1233 751 1693 2702
Fragments arranged by size:
2702 1693 1233 751 711
BsaBI GATnn'nnATC
Cuts at: 2484 2604 7084 2484
Size: 120 4480 2490
Fragments arranged by size:
4480 2490 120
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2793 4897 5279 5914
Size: 53 83 186 936 1260 2104 382 635
Cuts at: 5914 275
Size: 1451
Fragments arranged by size:
2104 1451 1260 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2892 3310 4229 5523 5921 6195 6342 6753 2892
Size: 418 919 1294 398 274 147 411 3229
Fragments arranged by size:
3229 1294 919 418 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2134 2164 1178
Size: 30 165 30 731 30 6104
Fragments arranged by size:
6104 731 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 3864 4280 1186
Size: 1111 1567 416 3996
Fragments arranged by size:
3996 1567 1111 416
BseYI C'CCAG_C
Cuts at: 2784 3018 3407 4140 6293 6789 2784
Size: 234 389 733 2153 496 3085
Fragments arranged by size:
3085 2153 733 496 389 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2897 3837 1163
Size: 573 1161 940 4416
Fragments arranged by size:
4416 1161 940 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4540 5301 6329 1540
Size: 275 186 2539 761 1028 2301
Fragments arranged by size:
2539 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5151 5236 6307 5151
Size: 85 1071 5934
Fragments arranged by size:
5934 1071 85
BsmI GAATG_Cn'
Cuts at: 2581 3367 4601 4694 2581
Size: 786 1234 93 4977
Fragments arranged by size:
4977 1234 786 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2633 2925 3788 4409 5151 5236
Size: 399 330 513 292 863 621 742 85
Cuts at: 5236 6307 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 863 742 621 513 399 330
292 85
BspEI T'CCGG_A
Cuts at: 2892 6753 2892
Size: 3861 3229
BspHI T'CATG_A
Cuts at: 4979 6709 4979
Size: 1730 5360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2919 4445 844
Size: 2075 1526 3489
Fragments arranged by size:
3489 2075 1526
BsrBI CCG'CTC
Cuts at: 1394 1823 4977 1394
Size: 429 3154 3507
Fragments arranged by size:
3507 3154 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2345 5585 5759 65
Size: 1183 1097 3240 174 1396
Fragments arranged by size:
3240 1396 1183 1097 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5734 5921 1700
Size: 110 3924 187 2869
Fragments arranged by size:
3924 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7090
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7090
BssSI C'ACGA_G
Cuts at: 5150 6162 5150
Size: 1012 6078
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2167 3551 3560 794
Size: 1373 1384 9 4324
Fragments arranged by size:
4324 1384 1373 9
BstBI TT'CG_AA
Cuts at: 5894 5894
Size: 7090
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7090
BstYI r'GATC_y
Cuts at: 1972 3068 3613 4420 4787 5172 5189 6641 6727
Size: 1096 545 807 367 385 17 1452 86
Cuts at: 6727 6739 7085 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1096 807 545 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2207 2207
Size: 7090
BtgI C'CryG_G
Cuts at: 513 1065 2004 2872 3375 3733 3857 3977 3987
Size: 552 939 868 503 358 124 120 10
Cuts at: 3987 4207 513
Size: 220 3396
Fragments arranged by size:
3396 939 868 552 503 358 220 124
120 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3159 487
Size: 683 56 1933 4418
Fragments arranged by size:
4418 1933 683 56
BtsI GCAGTG_nn'
Cuts at: 3006 4606 5400 5420 3006
Size: 1600 794 20 4676
Fragments arranged by size:
4676 1600 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7090
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3784 3819 529
Size: 35 3220 35 3800
Fragments arranged by size:
3800 3220 35 35
DraI TTT'AAA
Cuts at: 2175 4394 2175
Size: 2219 4871
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6097 809
Size: 507 4781 1802
Fragments arranged by size:
4781 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2619 2812 3372
Size: 54 1425 50 275 18 789 193 560
Cuts at: 3372 4037 4537 5426 7055 8
Size: 665 500 889 1629 43
Fragments arranged by size:
1629 1425 889 789 665 560 500 275
193 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4537 1537
Size: 275 2725 4090
Fragments arranged by size:
4090 2725 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2122 2907 3234 4110 4257 4281 4335 5020
Size: 769 785 327 876 147 24 54 685
Cuts at: 5020 1353
Size: 3423
Fragments arranged by size:
3423 876 785 769 685 327 147 54
24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5677 6051 6197 387
Size: 1064 138 4088 374 146 1280
Fragments arranged by size:
4088 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2603 3606 3984 4061 4238 4295
Size: 393 491 480 1003 378 77 177 57
Cuts at: 4295 5153 5750 6537 6805 1239
Size: 858 597 787 268 1524
Fragments arranged by size:
1524 1003 858 787 597 491 480 393
378 268 177 77 57
EcoNI CCTnn'n_nnAGG
Cuts at: 3233 3233
Size: 7090
EcoO109I rG'GnC_Cy
Cuts at: 3244 3380 3962 3244
Size: 136 582 6372
Fragments arranged by size:
6372 582 136
EcoRI G'AATT_C
Cuts at: 4399 4777 4399
Size: 378 6712
FspI TGC'GCA
Cuts at: 2862 5596 2862
Size: 2734 4356
HaeII r_GCGC'y
Cuts at: 1678 2796 6237 1678
Size: 1118 3441 2531
Fragments arranged by size:
3441 2531 1118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3150 3182 3949 3981 5731
Size: 32 135 32 1578 32 767 32 1750
Cuts at: 5731 5763 5805 5837 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1750 1578 767 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2956 4434 4680 669
Size: 988 281 1018 1478 246 3079
Fragments arranged by size:
3079 1478 1018 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7090
HpaI GTT'AAC
Cuts at: 4680 4680
Size: 7090
KasI G'GCGC_C
Cuts at: 2792 2792
Size: 7090
KpnI G_GTAC'C
Cuts at: 4415 4415
Size: 7090
MfeI C'AATT_G
Cuts at: 2948 4689 2948
Size: 1741 5349
MluI A'CGCG_T
Cuts at: 6966 6966
Size: 7090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2386 6204 6388 1305
Size: 1081 3818 184 2007
Fragments arranged by size:
3818 2007 1081 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2621 2814 10
Size: 54 1425 343 789 193 4286
Fragments arranged by size:
4286 1425 789 343 193 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2567 3113 3732 5048 5407 5566 518
Size: 648 1401 546 619 1316 359 159 2042
Fragments arranged by size:
2042 1401 1316 648 619 546 359 159
MspA1I CmG'CkG
Cuts at: 1606 2332 2965 2980 4189 4506 5183 6331 6576
Size: 726 633 15 1209 317 677 1148 245
Cuts at: 6576 1606
Size: 2120
Fragments arranged by size:
2120 1209 1148 726 677 633 317 245
15
NarI GG'CG_CC
Cuts at: 2793 2793
Size: 7090
NcoI C'CATG_G
Cuts at: 513 1065 2004 2872 3375 3733 3857 513
Size: 552 939 868 503 358 124 3746
Fragments arranged by size:
3746 939 868 552 503 358 124
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7090
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7090
NotI GC'GGCC_GC
Cuts at: 4537 4537
Size: 7090
NspI r_CATG'y
Cuts at: 1132 1229 2291 4448 5993 1132
Size: 97 1062 2157 1545 2229
Fragments arranged by size:
2229 2157 1545 1062 97
PasI CC'CwG_GG
Cuts at: 1348 2918 1348
Size: 1570 5520
PciI A'CATG_T
Cuts at: 5989 5989
Size: 7090
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2965 1108
Size: 1857 5233
PmeI GTTT'AAAC
Cuts at: 4394 4394
Size: 7090
PmlI CAC'GTG
Cuts at: 3188 3188
Size: 7090
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5159 5191 6672 6704 5159
Size: 32 1481 32 5545
Fragments arranged by size:
5545 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3244 3380 3244
Size: 136 6954
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7090
PsiI TTA'TAA
Cuts at: 4660 4660
Size: 7090
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2657 2689 4175 4207 2657
Size: 32 1486 32 5540
Fragments arranged by size:
5540 1486 32 32
PstI C_TGCA'G
Cuts at: 830 4442 830
Size: 3612 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7090
PvuII CAG'CTG
Cuts at: 2332 2965 2980 2332
Size: 633 15 6442
Fragments arranged by size:
6442 633 15
SalI G'TCGA_C
Cuts at: 1936 4432 1936
Size: 2496 4594
SapI GCTCTTCn'nnn_
Cuts at: 4335 4335
Size: 7090
SbfI CC_TGCA'GG
Cuts at: 4442 4442
Size: 7090
ScaI AGT'ACT
Cuts at: 2671 5338 7050 2671
Size: 2667 1712 2711
Fragments arranged by size:
2711 2667 1712
SfcI C'TryA_G
Cuts at: 826 1045 2457 4438 5573 6254 6445 826
Size: 219 1412 1981 1135 681 191 1471
Fragments arranged by size:
1981 1471 1412 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2794 2794
Size: 7090
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7090
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7090
SmaI CCC'GGG
Cuts at: 1872 4417 1872
Size: 2545 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3540 4199 5194 6095 6357 820
Size: 197 926 1597 659 995 901 262 1553
Fragments arranged by size:
1597 1553 995 926 901 659 262 197
SnaBI TAC'GTA
Cuts at: 493 4881 493
Size: 4388 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7090
SphI G_CATG'C
Cuts at: 1132 2291 4448 1132
Size: 1159 2157 3774
Fragments arranged by size:
3774 2157 1159
SspI AAT'ATT
Cuts at: 5 52 5014 5
Size: 47 4962 2081
Fragments arranged by size:
4962 2081 47
StuI AGG'CCT
Cuts at: 1517 3840 1517
Size: 2323 4767
StyI C'CwwG_G
Cuts at: 513 1065 2004 2622 2872 3375 3733 3802 3857
Size: 552 939 618 250 503 358 69 55
Cuts at: 3857 4065 4529 513
Size: 208 464 3074
Fragments arranged by size:
3074 939 618 552 503 464 358 250
208 69 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3342 3421 5117 5134 5287 5472 5917
Size: 1255 1762 79 1696 17 153 185 445
Cuts at: 5917 325
Size: 1498
Fragments arranged by size:
1762 1696 1498 1255 445 185 153 79
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2144 2669 4158
Size: 274 80 33 51 1443 167 525 1489
Cuts at: 4158 5336 7048 96
Size: 1178 1712 138
Fragments arranged by size:
1712 1489 1443 1178 525 274 167 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2134 2166 7050 7082 2134
Size: 32 4884 32 2142
Fragments arranged by size:
4884 2142 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4132 1316
Size: 2816 4274
XbaI T'CTAG_A
Cuts at: 3818 4426 3818
Size: 608 6482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7090
XmaI C'CCGG_G
Cuts at: 1870 4415 1870
Size: 2545 4545
XmnI GAAnn'nnTTC
Cuts at: 5219 5219
Size: 7090
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4898 276
Size: 53 83 186 936 3364 2468
Fragments arranged by size:
3364 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI
SrfI SwaI XcmI