Restriction map for
pFN21AB5122
(Circular) (Six-base) MAPSORT of: pFN21AB5122.seq Check: 1031 from: 1 to: 6247
pFN21AB5122 6247 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4057 278
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2173 2230 2647 2862 3291 3590 1937
Size: 236 57 417 215 429 299 4594
Fragments arranged by size:
4594 429 417 299 236 215 57
Acc65I G'GTAC_C
Cuts at: 2213 3568 2213
Size: 1355 4892
AclI AA'CG_TT
Cuts at: 1563 4374 4747 1563
Size: 2811 373 3063
Fragments arranged by size:
3063 2811 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2535 4310 5694 5962 1632
Size: 903 1775 1384 268 1917
Fragments arranged by size:
1917 1775 1384 903 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6050
AflIII A'CryG_T
Cuts at: 1203 5146 6123 1203
Size: 3943 977 1327
Fragments arranged by size:
3943 1327 977
AgeI A'CCGG_T
Cuts at: 5078 5078
Size: 6247
AhdI GACnn_n'nnGTC
Cuts at: 4976 4976
Size: 6247
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3441 1184
Size: 2257 3990
AlwNI CAG_nnn'CTG
Cuts at: 2333 2336 2567 3990 5562 2333
Size: 3 231 1423 1572 3018
Fragments arranged by size:
3018 1572 1423 231 3
ApaLI G'TGCA_C
Cuts at: 4304 5460 4304
Size: 1156 5091
ApoI r'AATT_y
Cuts at: 1493 2839 3183 3556 3782 3934 6043 1493
Size: 1346 344 373 226 152 2109 1697
Fragments arranged by size:
2109 1697 1346 373 344 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2202 4801 160
Size: 872 1170 2599 1606
Fragments arranged by size:
2599 1606 1170 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
AvaI C'yCGr_G
Cuts at: 1870 1943 3572 1870
Size: 73 1629 4545
Fragments arranged by size:
4545 1629 73
BamHI G'GATC_C
Cuts at: 3577 3944 3577
Size: 367 5880
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2126 2213 3568 5023 618
Size: 325 217 627 339 87 1355 1455 1842
Fragments arranged by size:
1842 1455 1355 627 339 325 217 87
BanII G_rGCy'C
Cuts at: 1391 3566 1391
Size: 2175 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3285 3319 4438 4472 1903
Size: 34 1348 34 1119 34 3678
Fragments arranged by size:
3678 1348 1119 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4139 5349 4139
Size: 1210 5037
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6247
BfrBI ATG'CAT
Cuts at: 3306 3306
Size: 6247
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4858 136
Size: 107 122 71 631 605 3186 1525
Fragments arranged by size:
3186 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6242 6242
Size: 6247
BlpI GC'TnA_GC
Cuts at: 3664 3664
Size: 6247
Bme1580I G_kGCm'C
Cuts at: 1790 2131 4308 5464 1790
Size: 341 2177 1156 2573
Fragments arranged by size:
2573 2177 1156 341
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6247
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3978 4936 442
Size: 416 53 83 399 24 2561 958 1753
Fragments arranged by size:
2561 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2784 1056
Size: 1728 4519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2589 2877 4907 1239
Size: 1350 288 2030 2579
Fragments arranged by size:
2579 2030 1350 288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4372 5237 5535 4372
Size: 865 298 5084
Fragments arranged by size:
5084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4910 882
Size: 617 3411 2219
Fragments arranged by size:
3411 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4038 493
Size: 711 2834 2702
Fragments arranged by size:
2834 2702 711
BsaBI GATnn'nnATC
Cuts at: 2156 3305 6241 2156
Size: 1149 2936 2162
Fragments arranged by size:
2936 2162 1149
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4054 4436 5071 275
Size: 53 83 186 936 2521 382 635 1451
Fragments arranged by size:
2521 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4680 5078 5352 5499 5910 4680
Size: 398 274 147 411 5017
Fragments arranged by size:
5017 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3480 3510 1178
Size: 30 165 30 2077 30 3915
Fragments arranged by size:
3915 2077 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 1186
Size: 1234 5013
BseYI C'CCAG_C
Cuts at: 5450 5946 5450
Size: 496 5751
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5674
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3697 4458 5486 1540
Size: 275 186 1696 761 1028 2301
Fragments arranged by size:
2301 1696 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2419 3430 4308 4393 5464 2419
Size: 1011 878 85 1071 3202
Fragments arranged by size:
3202 1071 1011 878 85
BsmI GAATG_Cn'
Cuts at: 2994 3484 3758 3851 2994
Size: 490 274 93 5390
Fragments arranged by size:
5390 490 274 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2131 2419 3430 3566 4308 4393 5464
Size: 399 341 288 1011 136 742 85 1071
Cuts at: 5464 1391
Size: 2174
Fragments arranged by size:
2174 1071 1011 742 399 341 288 136
85
BspEI T'CCGG_A
Cuts at: 5910 5910
Size: 6247
BspHI T'CATG_A
Cuts at: 4136 5866 4136
Size: 1730 4517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3357 3602 844
Size: 2513 245 3489
Fragments arranged by size:
3489 2513 245
BsrBI CCG'CTC
Cuts at: 1394 1823 4134 1394
Size: 429 2311 3507
Fragments arranged by size:
3507 2311 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4742 4916 65
Size: 1183 3494 174 1396
Fragments arranged by size:
3494 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4891 5078 1700
Size: 110 3081 187 2869
Fragments arranged by size:
3081 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3339 3400 96
Size: 3243 61 2943
Fragments arranged by size:
3243 2943 61
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6247
BssSI C'ACGA_G
Cuts at: 4307 5319 4307
Size: 1012 5235
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2333 2336 794
Size: 1539 3 4705
Fragments arranged by size:
4705 1539 3
BstBI TT'CG_AA
Cuts at: 5051 5051
Size: 6247
BstEII G'GTnAC_C
Cuts at: 1184 2771 1184
Size: 1587 4660
BstYI r'GATC_y
Cuts at: 1972 2369 3577 3944 4329 4346 5798 5884 5896
Size: 397 1208 367 385 17 1452 86 12
Cuts at: 5896 6242 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1208 397 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2648 2648
Size: 6247
Bsu36I CC'TnA_GG
Cuts at: 2788 2788
Size: 6247
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5695
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5508
Fragments arranged by size:
5508 683 56
BtsI GCAGTG_nn'
Cuts at: 2023 3763 4557 4577 2023
Size: 1740 794 20 3693
Fragments arranged by size:
3693 1740 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6212
DraI TTT'AAA
Cuts at: 3123 3551 3123
Size: 428 5819
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3152 5254 809
Size: 507 1836 2102 1802
Fragments arranged by size:
2102 1836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3694 4583 6212
Size: 54 1425 50 275 18 1864 889 1629
Cuts at: 6212 8
Size: 43
Fragments arranged by size:
1864 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3694 1537
Size: 275 1882 4090
Fragments arranged by size:
4090 1882 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4177 1353
Size: 2824 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4834 5208 5354 387
Size: 1064 138 3245 374 146 1280
Fragments arranged by size:
3245 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2535 2589 2877 4310 4907 5694 5962
Size: 393 903 54 288 1433 597 787 268
Cuts at: 5962 1239
Size: 1524
Fragments arranged by size:
1524 1433 903 787 597 393 288 268
54
EcoO109I rG'GnC_Cy
Cuts at: 2804 3076 2804
Size: 272 5975
EcoRI G'AATT_C
Cuts at: 3556 3934 3556
Size: 378 5869
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2735 3402 2735
Size: 667 5580
FspI TGC'GCA
Cuts at: 4753 4753
Size: 6247
HaeII r_GCGC'y
Cuts at: 1678 5394 1678
Size: 3716 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2231 2263 2675 2707 3211
Size: 32 135 32 659 32 412 32 504
Cuts at: 3211 3243 3412 3444 4888 4920 4962 4994 1373
Size: 32 169 32 1444 32 42 32 2626
Fragments arranged by size:
2626 1444 659 504 412 169 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2029 2231 3591 3837 669
Size: 988 281 91 202 1360 246 3079
Fragments arranged by size:
3079 1360 988 281 246 202 91
HindIII A'AGCT_T
Cuts at: 748 2295 2309 748
Size: 1547 14 4686
Fragments arranged by size:
4686 1547 14
HpaI GTT'AAC
Cuts at: 3837 3837
Size: 6247
KpnI G_GTAC'C
Cuts at: 2217 3572 2217
Size: 1355 4892
MfeI C'AATT_G
Cuts at: 3148 3846 3148
Size: 698 5549
MluI A'CGCG_T
Cuts at: 6123 6123
Size: 6247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3230 3478 5361 5545 1305
Size: 1925 248 1883 184 2007
Fragments arranged by size:
2007 1925 1883 248 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4425
Fragments arranged by size:
4425 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2821 2895 3051 4205 4564 4723 518
Size: 648 1655 74 156 1154 359 159 2042
Fragments arranged by size:
2042 1655 1154 648 359 159 156 74
MspA1I CmG'CkG
Cuts at: 1606 2144 2333 2342 2351 3663 4340 5488 5733
Size: 538 189 9 9 1312 677 1148 245
Cuts at: 5733 1606
Size: 2120
Fragments arranged by size:
2120 1312 1148 677 538 245 189 9
9
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5695
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6247
NheI G'CTAG_C
Cuts at: 1052 2780 1052
Size: 1728 4519
NotI GC'GGCC_GC
Cuts at: 3694 3694
Size: 6247
NsiI A_TGCA'T
Cuts at: 3308 3308
Size: 6247
NspI r_CATG'y
Cuts at: 1132 1229 3605 5150 1132
Size: 97 2376 1545 2229
Fragments arranged by size:
2376 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6247
PciI A'CATG_T
Cuts at: 5146 5146
Size: 6247
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2757 1108
Size: 1649 4598
PmeI GTTT'AAAC
Cuts at: 3551 3551
Size: 6247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4316 4348 5829 5861 4316
Size: 32 1481 32 4702
Fragments arranged by size:
4702 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3076 3076
Size: 6247
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6247
PsiI TTA'TAA
Cuts at: 3817 3817
Size: 6247
PstI C_TGCA'G
Cuts at: 830 2341 2356 3599 830
Size: 1511 15 1243 3478
Fragments arranged by size:
3478 1511 1243 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6247
PvuII CAG'CTG
Cuts at: 2333 2342 2351 2333
Size: 9 9 6229
Fragments arranged by size:
6229 9 9
SalI G'TCGA_C
Cuts at: 1936 2229 3589 1936
Size: 293 1360 4594
Fragments arranged by size:
4594 1360 293
SanDI GG'GwC_CC
Cuts at: 3076 3076
Size: 6247
SbfI CC_TGCA'GG
Cuts at: 3599 3599
Size: 6247
ScaI AGT'ACT
Cuts at: 2634 4495 6207 2634
Size: 1861 1712 2674
Fragments arranged by size:
2674 1861 1712
SfcI C'TryA_G
Cuts at: 826 1045 2174 2337 2352 2424 3442 3595 4730
Size: 219 1129 163 15 72 1018 153 1135
Cuts at: 4730 5411 5602 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1129 1018 681 219 191 163
153 72 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6247
SmaI CCC'GGG
Cuts at: 1872 3574 1872
Size: 1702 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4351 5252 5514 820
Size: 197 926 2408 901 262 1553
Fragments arranged by size:
2408 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4038 493
Size: 3545 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6247
SphI G_CATG'C
Cuts at: 1132 3605 1132
Size: 2473 3774
SspI AAT'ATT
Cuts at: 5 52 3462 4171 5
Size: 47 3410 709 2081
Fragments arranged by size:
3410 2081 709 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6247
StyI C'CwwG_G
Cuts at: 513 1065 3040 3686 513
Size: 552 1975 646 3074
Fragments arranged by size:
3074 1975 646 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3309 4274 4291 4444 4629 5074 325
Size: 1255 1729 965 17 153 185 445 1498
Fragments arranged by size:
1729 1498 1255 965 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2278 2632 2981
Size: 274 80 33 51 1443 301 354 349
Cuts at: 2981 3339 3400 4493 6205 96
Size: 358 61 1093 1712 138
Fragments arranged by size:
1712 1443 1093 358 354 349 301 274
138 80 61 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2584 2616 6207 6239 2584
Size: 32 3591 32 2592
Fragments arranged by size:
3591 2592 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6247
XbaI T'CTAG_A
Cuts at: 2695 3583 2695
Size: 888 5359
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6247
XmaI C'CCGG_G
Cuts at: 1870 3572 1870
Size: 1702 4545
XmnI GAAnn'nnTTC
Cuts at: 4376 4376
Size: 6247
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4055 276
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI
XcmI