Restriction map for
pFN21AB5120
(Circular) (Six-base) MAPSORT of: pFN21AB5120.seq Check: 286 from: 1 to: 5965
pFN21AB5120 5965 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3082 3082
Size: 5965
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3775 278
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2421 2972 3308 1937
Size: 484 551 336 4594
Fragments arranged by size:
4594 551 484 336
Acc65I G'GTAC_C
Cuts at: 3286 3286
Size: 5965
AclI AA'CG_TT
Cuts at: 1563 4092 4465 1563
Size: 2529 373 3063
Fragments arranged by size:
3063 2529 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2049 4028 5412 5680 1632
Size: 417 1979 1384 268 1917
Fragments arranged by size:
1979 1917 1384 417 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5768
AflIII A'CryG_T
Cuts at: 1203 4864 5841 1203
Size: 3661 977 1327
Fragments arranged by size:
3661 1327 977
AgeI A'CCGG_T
Cuts at: 4796 4796
Size: 5965
AhdI GACnn_n'nnGTC
Cuts at: 4694 4694
Size: 5965
AleI CACnn'nnGTG
Cuts at: 1166 2183 1166
Size: 1017 4948
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2069 1184
Size: 885 5080
AlwNI CAG_nnn'CTG
Cuts at: 3708 5280 3708
Size: 1572 4393
ApaLI G'TGCA_C
Cuts at: 3009 4022 5178 3009
Size: 1013 1156 3796
Fragments arranged by size:
3796 1156 1013
ApoI r'AATT_y
Cuts at: 1493 2873 2879 3274 3500 3652 5761 1493
Size: 1380 6 395 226 152 2109 1697
Fragments arranged by size:
2109 1697 1380 395 226 152 6
AseI AT'TA_AT
Cuts at: 160 1032 4519 160
Size: 872 3487 1606
Fragments arranged by size:
3487 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
AvaI C'yCGr_G
Cuts at: 1870 1943 3290 1870
Size: 73 1347 4545
Fragments arranged by size:
4545 1347 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2571 2604 2571
Size: 33 5932
BamHI G'GATC_C
Cuts at: 2572 3295 3662 2572
Size: 723 367 4875
Fragments arranged by size:
4875 723 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2674 3286 4741 618
Size: 325 217 627 887 612 1455 1842
Fragments arranged by size:
1842 1455 887 627 612 325 217
BanII G_rGCy'C
Cuts at: 1391 2298 3284 1391
Size: 907 986 4072
Fragments arranged by size:
4072 986 907
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2908 928
Size: 971 1009 3985
Fragments arranged by size:
3985 1009 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4156 4190 1903
Size: 34 2219 34 3678
Fragments arranged by size:
3678 2219 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3857 5067 3857
Size: 1210 4755
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5965
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4576 136
Size: 107 122 71 631 605 2904 1525
Fragments arranged by size:
2904 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5960 5960
Size: 5965
BlpI GC'TnA_GC
Cuts at: 2748 3382 2748
Size: 634 5331
Bme1580I G_kGCm'C
Cuts at: 1790 2677 3013 4026 5182 1790
Size: 887 336 1013 1156 2573
Fragments arranged by size:
2573 1156 1013 887 336
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5965
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3696 4654 442
Size: 416 53 83 399 24 2279 958 1753
Fragments arranged by size:
2279 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2282 2341 2987 3231 4625 1239
Size: 1043 59 646 244 1394 2579
Fragments arranged by size:
2579 1394 1043 646 244 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3081 4090 4955 5253 3081
Size: 1009 865 298 3793
Fragments arranged by size:
3793 1009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3207 4628 882
Size: 617 1708 1421 2219
Fragments arranged by size:
2219 1708 1421 617
BsaAI yAC'GTr
Cuts at: 493 1204 3756 493
Size: 711 2552 2702
Fragments arranged by size:
2702 2552 711
BsaBI GATnn'nnATC
Cuts at: 5959 5959
Size: 5965
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3772 4154 4789 275
Size: 53 83 186 936 2239 382 635 1451
Fragments arranged by size:
2239 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2410 2935 4398 4796 5070 5217 5628 2410
Size: 525 1463 398 274 147 411 2747
Fragments arranged by size:
2747 1463 525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2232 2262 2445 2475 1178
Size: 30 165 30 829 30 183 30 4668
Fragments arranged by size:
4668 829 183 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2646 3132 1186
Size: 1460 486 4019
Fragments arranged by size:
4019 1460 486
BseYI C'CCAG_C
Cuts at: 5168 5664 5168
Size: 496 5469
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2075 3060 1163
Size: 573 339 985 4068
Fragments arranged by size:
4068 985 573 339
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3415 4176 5204 1540
Size: 275 186 1414 761 1028 2301
Fragments arranged by size:
2301 1414 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2298 3013 4026 4111 5182 2298
Size: 715 1013 85 1071 3081
Fragments arranged by size:
3081 1071 1013 715 85
BsmI GAATG_Cn'
Cuts at: 3476 3569 3476
Size: 93 5872
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 2677 3013 3284 4026 4111 5182
Size: 399 508 379 336 271 742 85 1071
Cuts at: 5182 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 508 399 379 336 271
85
BspEI T'CCGG_A
Cuts at: 2410 5628 2410
Size: 3218 2747
BspHI T'CATG_A
Cuts at: 2304 3854 5584 2304
Size: 1550 1730 2685
Fragments arranged by size:
2685 1730 1550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3082 3320 844
Size: 2238 238 3489
Fragments arranged by size:
3489 2238 238
BsrBI CCG'CTC
Cuts at: 1394 1823 3852 1394
Size: 429 2029 3507
Fragments arranged by size:
3507 2029 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4460 4634 65
Size: 1183 3212 174 1396
Fragments arranged by size:
3212 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2050 4609 4796 1700
Size: 110 240 2559 187 2869
Fragments arranged by size:
2869 2559 240 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5965
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5965
BssSI C'ACGA_G
Cuts at: 4025 5037 4025
Size: 1012 4953
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5965
BstBI TT'CG_AA
Cuts at: 4769 4769
Size: 5965
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5965
BstXI CCAn_nnnn'nTGG
Cuts at: 2528 2528
Size: 5965
BstYI r'GATC_y
Cuts at: 1972 2572 3295 3662 4047 4064 5516 5602 5614
Size: 600 723 367 385 17 1452 86 12
Cuts at: 5614 5960 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 723 600 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2422 2973 2422
Size: 551 5414
BtgI C'CryG_G
Cuts at: 513 1065 3217 513
Size: 552 2152 3261
Fragments arranged by size:
3261 2152 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5226
Fragments arranged by size:
5226 683 56
BtsI GCAGTG_nn'
Cuts at: 3481 4275 4295 3481
Size: 794 20 5151
Fragments arranged by size:
5151 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2014 2049 2106 2141 529
Size: 35 1450 35 57 35 4353
Fragments arranged by size:
4353 1450 57 35 35 35
DraI TTT'AAA
Cuts at: 2878 3269 2878
Size: 391 5574
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2442 3044 4972 809
Size: 507 1126 602 1928 1802
Fragments arranged by size:
1928 1802 1126 602 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3412 4301 5930
Size: 54 1425 50 275 18 1582 889 1629
Cuts at: 5930 8
Size: 43
Fragments arranged by size:
1629 1582 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3412 1537
Size: 275 1600 4090
Fragments arranged by size:
4090 1600 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3086 3895 1353
Size: 1733 809 3423
Fragments arranged by size:
3423 1733 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4552 4926 5072 387
Size: 1064 138 2963 374 146 1280
Fragments arranged by size:
2963 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2296 2296
Size: 5965
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2282 2341 2987 3231 4028 4625
Size: 393 417 233 59 646 244 797 597
Cuts at: 4625 5412 5680 1239
Size: 787 268 1524
Fragments arranged by size:
1524 797 787 646 597 417 393 268
244 233 59
EcoO109I rG'GnC_Cy
Cuts at: 2818 3037 2818
Size: 219 5746
EcoRI G'AATT_C
Cuts at: 3274 3652 3274
Size: 378 5587
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2243 2243
Size: 5965
FspI TGC'GCA
Cuts at: 4471 4471
Size: 5965
HaeII r_GCGC'y
Cuts at: 1678 5112 1678
Size: 3434 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2232 2264 2693 2711 2725
Size: 32 135 32 660 32 429 18 14
Cuts at: 2725 2743 4606 4638 4680 4712 1373
Size: 18 1863 32 42 32 2626
Fragments arranged by size:
2626 1863 660 429 135 42 32 32
32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3309 3555 669
Size: 988 281 1371 246 3079
Fragments arranged by size:
3079 1371 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3003 748
Size: 2255 3710
HpaI GTT'AAC
Cuts at: 3555 3555
Size: 5965
KpnI G_GTAC'C
Cuts at: 3290 3290
Size: 5965
MfeI C'AATT_G
Cuts at: 2943 3564 2943
Size: 621 5344
MluI A'CGCG_T
Cuts at: 5841 5841
Size: 5965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2550 2855 2944 3279 5079 5263 1305
Size: 1245 305 89 335 1800 184 2007
Fragments arranged by size:
2007 1800 1245 335 305 184 89
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4143
Fragments arranged by size:
4143 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2183 3923 4282 4441 518
Size: 648 1017 1740 359 159 2042
Fragments arranged by size:
2042 1740 1017 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2497 2623 3164 3381 4058 5206 5451 1606
Size: 891 126 541 217 677 1148 245 2120
Fragments arranged by size:
2120 1148 891 677 541 245 217 126
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5413
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5965
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5965
NotI GC'GGCC_GC
Cuts at: 3412 3412
Size: 5965
NspI r_CATG'y
Cuts at: 1132 1229 3323 4868 1132
Size: 97 2094 1545 2229
Fragments arranged by size:
2229 2094 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5965
PciI A'CATG_T
Cuts at: 4864 4864
Size: 5965
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5965
PmeI GTTT'AAAC
Cuts at: 3269 3269
Size: 5965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4034 4066 5547 5579 4034
Size: 32 1481 32 4420
Fragments arranged by size:
4420 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2818 3037 2818
Size: 219 5746
PshAI GACnn'nnGTC
Cuts at: 1613 2912 1613
Size: 1299 4666
PsiI TTA'TAA
Cuts at: 3535 3535
Size: 5965
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2483 2515 2849 2881 2483
Size: 32 334 32 5567
Fragments arranged by size:
5567 334 32 32
PstI C_TGCA'G
Cuts at: 830 3317 830
Size: 2487 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5965
PvuII CAG'CTG
Cuts at: 2497 2623 3164 2497
Size: 126 541 5298
Fragments arranged by size:
5298 541 126
SacI G_AGCT'C
Cuts at: 2298 2298
Size: 5965
SalI G'TCGA_C
Cuts at: 1936 3307 1936
Size: 1371 4594
SanDI GG'GwC_CC
Cuts at: 3037 3037
Size: 5965
SbfI CC_TGCA'GG
Cuts at: 3317 3317
Size: 5965
ScaI AGT'ACT
Cuts at: 4213 5925 4213
Size: 1712 4253
SfcI C'TryA_G
Cuts at: 826 1045 2440 2668 3313 4448 5129 5320 826
Size: 219 1395 228 645 1135 681 191 1471
Fragments arranged by size:
1471 1395 1135 681 645 228 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5965
SmaI CCC'GGG
Cuts at: 1872 3292 1872
Size: 1420 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3096 4069 4970 5232 820
Size: 197 926 1153 973 901 262 1553
Fragments arranged by size:
1553 1153 973 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3756 493
Size: 3263 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5965
SphI G_CATG'C
Cuts at: 1132 3323 1132
Size: 2191 3774
SspI AAT'ATT
Cuts at: 5 52 2772 3889 5
Size: 47 2720 1117 2081
Fragments arranged by size:
2720 2081 1117 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5965
StyI C'CwwG_G
Cuts at: 513 1065 2903 3404 513
Size: 552 1838 501 3074
Fragments arranged by size:
3074 1838 552 501
SwaI ATTT'AAAT
Cuts at: 2878 2878
Size: 5965
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3992 4009 4162 4347 4792 325
Size: 1255 2412 17 153 185 445 1498
Fragments arranged by size:
2412 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4211 5923 96
Size: 274 80 33 51 1443 2234 1712 138
Fragments arranged by size:
2234 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5925 5957 5925
Size: 32 5933
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5965
XbaI T'CTAG_A
Cuts at: 3301 3301
Size: 5965
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5965
XmaI C'CCGG_G
Cuts at: 1870 3290 1870
Size: 1420 4545
XmnI GAAnn'nnTTC
Cuts at: 4094 4094
Size: 5965
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3773 276
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI RsrII SacII SapI
SexAI SfiI SfoI SrfI XcmI