(Circular) (Six-base) MAPSORT of: pFN21AB5105.seq Check: 7506 from: 1 to: 6556 pFN21AB5105 6556 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4366 278 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3899 1937 Size: 1962 4594 Acc65I G'GTAC_C Cuts at: 3877 3877 Size: 6556 AclI AA'CG_TT Cuts at: 1563 4683 5056 1563 Size: 3120 373 3063 Fragments arranged by size: 3120 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2880 4619 6003 6271 1632 Size: 1248 1739 1384 268 1917 Fragments arranged by size: 1917 1739 1384 1248 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6359 AflIII A'CryG_T Cuts at: 1203 2373 5455 6432 1203 Size: 1170 3082 977 1327 Fragments arranged by size: 3082 1327 1170 977 AgeI A'CCGG_T Cuts at: 5387 5387 Size: 6556 AhdI GACnn_n'nnGTC Cuts at: 5285 5285 Size: 6556 AleI CACnn'nnGTG Cuts at: 1166 2618 1166 Size: 1452 5104 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3392 1184 Size: 2208 4348 AlwNI CAG_nnn'CTG Cuts at: 2084 3597 4299 5871 2084 Size: 1513 702 1572 2769 Fragments arranged by size: 2769 1572 1513 702 ApaLI G'TGCA_C Cuts at: 4613 5769 4613 Size: 1156 5400 ApoI r'AATT_y Cuts at: 1493 2588 3447 3758 3865 4091 4243 6352 1493 Size: 1095 859 311 107 226 152 2109 1697 Fragments arranged by size: 2109 1697 1095 859 311 226 152 107 AseI AT'TA_AT Cuts at: 160 1032 5110 160 Size: 872 4078 1606 Fragments arranged by size: 4078 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 AvaI C'yCGr_G Cuts at: 1870 1943 3881 1870 Size: 73 1938 4545 Fragments arranged by size: 4545 1938 73 BamHI G'GATC_C Cuts at: 3766 3886 4253 3766 Size: 120 367 6069 Fragments arranged by size: 6069 367 120 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3140 3877 5332 618 Size: 325 217 627 1353 737 1455 1842 Fragments arranged by size: 1842 1455 1353 737 627 325 217 BanII G_rGCy'C Cuts at: 1391 2886 3875 1391 Size: 1495 989 4072 Fragments arranged by size: 4072 1495 989 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3471 3828 928 Size: 971 1572 357 3656 Fragments arranged by size: 3656 1572 971 357 BbvCI CC'TCA_GC Cuts at: 3069 3069 Size: 6556 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4747 4781 1903 Size: 34 2810 34 3678 Fragments arranged by size: 3678 2810 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4448 5658 4448 Size: 1210 5346 BclI T'GATC_A Cuts at: 1548 1548 Size: 6556 BfrBI ATG'CAT Cuts at: 3836 3836 Size: 6556 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3149 5167 136 Size: 107 122 71 631 605 1477 2018 1525 Fragments arranged by size: 2018 1525 1477 631 605 122 107 71 BglII A'GATC_T Cuts at: 6551 6551 Size: 6556 BlpI GC'TnA_GC Cuts at: 3973 3973 Size: 6556 Bme1580I G_kGCm'C Cuts at: 1790 3145 4617 5773 1790 Size: 1355 1472 1156 2573 Fragments arranged by size: 2573 1472 1355 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6556 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4287 5245 442 Size: 416 53 83 399 24 2870 958 1753 Fragments arranged by size: 2870 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6556 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3752 Size: 6556 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2772 3551 5216 1239 Size: 1533 779 1665 2579 Fragments arranged by size: 2579 1665 1533 779 Bpu10I CC'TnA_GC Cuts at: 3069 3441 3069 Size: 372 6184 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4681 5546 5844 4681 Size: 865 298 5393 Fragments arranged by size: 5393 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2080 5219 882 Size: 617 581 3139 2219 Fragments arranged by size: 3139 2219 617 581 BsaAI yAC'GTr Cuts at: 493 1204 4347 493 Size: 711 3143 2702 Fragments arranged by size: 3143 2702 711 BsaBI GATnn'nnATC Cuts at: 6550 6550 Size: 6556 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4363 4745 5380 275 Size: 53 83 186 936 2830 382 635 1451 Fragments arranged by size: 2830 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4989 5387 5661 5808 6219 4989 Size: 398 274 147 411 5326 Fragments arranged by size: 5326 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2223 2253 2358 2388 1178 Size: 30 165 30 820 30 105 30 5346 Fragments arranged by size: 5346 820 165 105 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2379 1186 Size: 1193 5363 BseYI C'CCAG_C Cuts at: 5759 6255 5759 Size: 496 6060 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2133 1163 Size: 573 397 5586 Fragments arranged by size: 5586 573 397 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4006 4767 5795 1540 Size: 275 186 2005 761 1028 2301 Fragments arranged by size: 2301 2005 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2886 3723 4617 4702 5773 2886 Size: 837 894 85 1071 3669 Fragments arranged by size: 3669 1071 894 837 85 BsmI GAATG_Cn' Cuts at: 3836 3838 4067 4160 3836 Size: 2 229 93 6232 Fragments arranged by size: 6232 229 93 2 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2886 3145 3723 3875 4617 4702 5773 Size: 399 1096 259 578 152 742 85 1071 Cuts at: 5773 1391 Size: 2174 Fragments arranged by size: 2174 1096 1071 742 578 399 259 152 85 BspEI T'CCGG_A Cuts at: 6219 6219 Size: 6556 BspHI T'CATG_A Cuts at: 4445 6175 4445 Size: 1730 4826 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3911 844 Size: 3067 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4443 1394 Size: 429 2620 3507 Fragments arranged by size: 3507 2620 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2538 5051 5225 65 Size: 1183 1290 2513 174 1396 Fragments arranged by size: 2513 1396 1290 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5200 5387 1700 Size: 110 3390 187 2869 Fragments arranged by size: 3390 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3481 96 Size: 3385 3171 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6556 BssSI C'ACGA_G Cuts at: 4616 5628 4616 Size: 1012 5544 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6556 BstBI TT'CG_AA Cuts at: 5360 5360 Size: 6556 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6556 BstYI r'GATC_y Cuts at: 1972 2233 3766 3886 4253 4638 4655 6107 6193 Size: 261 1533 120 367 385 17 1452 86 Cuts at: 6193 6205 6551 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1533 1452 385 367 346 261 120 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2616 513 Size: 552 939 612 4453 Fragments arranged by size: 4453 939 612 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5817 Fragments arranged by size: 5817 683 56 BtsI GCAGTG_nn' Cuts at: 3675 4072 4866 4886 3675 Size: 397 794 20 5345 Fragments arranged by size: 5345 794 397 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6556 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6521 DraI TTT'AAA Cuts at: 3860 3860 Size: 6556 DraIII CAC_nnn'GTG Cuts at: 3245 3245 Size: 6556 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5563 809 Size: 507 4247 1802 Fragments arranged by size: 4247 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2692 4003 4892 Size: 54 1425 50 275 18 862 1311 889 Cuts at: 4892 6521 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1311 889 862 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4003 1537 Size: 275 2191 4090 Fragments arranged by size: 4090 2191 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2918 4486 1353 Size: 1565 1568 3423 Fragments arranged by size: 3423 1568 1565 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2466 5143 5517 5663 387 Size: 1064 138 877 2677 374 146 1280 Fragments arranged by size: 2677 1280 1064 877 374 146 138 EcoICRI GAG'CTC Cuts at: 2884 2884 Size: 6556 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2772 2880 3551 4619 5216 6003 6271 Size: 393 1140 108 671 1068 597 787 268 Cuts at: 6271 1239 Size: 1524 Fragments arranged by size: 1524 1140 1068 787 671 597 393 268 108 EcoO109I rG'GnC_Cy Cuts at: 2081 3073 2081 Size: 992 5564 EcoRI G'AATT_C Cuts at: 3865 4243 3865 Size: 378 6178 EcoRV GAT'ATC Cuts at: 2329 2395 2781 3125 2329 Size: 66 386 344 5760 Fragments arranged by size: 5760 386 344 66 FspI TGC'GCA Cuts at: 5062 5062 Size: 6556 HaeII r_GCGC'y Cuts at: 1678 5703 1678 Size: 4025 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3041 3073 5197 5229 5271 Size: 32 135 32 1469 32 2124 32 42 Cuts at: 5271 5303 1373 Size: 32 2626 Fragments arranged by size: 2626 2124 1469 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2688 3900 4146 669 Size: 988 281 750 1212 246 3079 Fragments arranged by size: 3079 1212 988 750 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6556 HpaI GTT'AAC Cuts at: 4146 4146 Size: 6556 KpnI G_GTAC'C Cuts at: 3881 3881 Size: 6556 MfeI C'AATT_G Cuts at: 3349 4155 3349 Size: 806 5750 MluI A'CGCG_T Cuts at: 6432 6432 Size: 6556 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2137 2209 2304 3654 5670 5854 1305 Size: 832 72 95 1350 2016 184 2007 Fragments arranged by size: 2016 2007 1350 832 184 95 72 MscI TGG'CCA Cuts at: 10 64 1489 1832 2694 10 Size: 54 1425 343 862 3872 Fragments arranged by size: 3872 1425 862 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2618 4514 4873 5032 518 Size: 648 1452 1896 359 159 2042 Fragments arranged by size: 2042 1896 1452 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3972 4649 5797 6042 1606 Size: 2366 677 1148 245 2120 Fragments arranged by size: 2366 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2616 513 Size: 552 939 612 4453 Fragments arranged by size: 4453 939 612 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6556 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6556 NotI GC'GGCC_GC Cuts at: 4003 4003 Size: 6556 NsiI A_TGCA'T Cuts at: 3838 3838 Size: 6556 NspI r_CATG'y Cuts at: 1132 1229 3829 3914 5459 1132 Size: 97 2600 85 1545 2229 Fragments arranged by size: 2600 2229 1545 97 85 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6556 PciI A'CATG_T Cuts at: 5455 5455 Size: 6556 PflMI CCAn_nnn'nTGG Cuts at: 1108 3245 1108 Size: 2137 4419 PfoI T'CCnGG_A Cuts at: 2868 2868 Size: 6556 PmeI GTTT'AAAC Cuts at: 3860 3860 Size: 6556 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4625 4657 6138 6170 4625 Size: 32 1481 32 5011 Fragments arranged by size: 5011 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2081 2081 Size: 6556 PshAI GACnn'nnGTC Cuts at: 1613 2320 1613 Size: 707 5849 PsiI TTA'TAA Cuts at: 4126 4126 Size: 6556 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2628 2660 2628 Size: 32 6524 PstI C_TGCA'G Cuts at: 830 3115 3456 3908 830 Size: 2285 341 452 3478 Fragments arranged by size: 3478 2285 452 341 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6556 SacI G_AGCT'C Cuts at: 2886 2886 Size: 6556 SalI G'TCGA_C Cuts at: 1936 3898 1936 Size: 1962 4594 SbfI CC_TGCA'GG Cuts at: 3908 3908 Size: 6556 ScaI AGT'ACT Cuts at: 3011 4804 6516 3011 Size: 1793 1712 3051 Fragments arranged by size: 3051 1793 1712 SexAI A'CCwGG_T Cuts at: 3241 3241 Size: 6556 SfcI C'TryA_G Cuts at: 826 1045 2265 3111 3364 3452 3904 5039 5720 Size: 219 1220 846 253 88 452 1135 681 Cuts at: 5720 5911 826 Size: 191 1471 Fragments arranged by size: 1471 1220 1135 846 681 452 253 219 191 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6556 SmaI CCC'GGG Cuts at: 1872 3883 1872 Size: 2011 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4660 5561 5823 820 Size: 197 926 2717 901 262 1553 Fragments arranged by size: 2717 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4347 493 Size: 3854 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6556 SphI G_CATG'C Cuts at: 1132 3914 1132 Size: 2782 3774 SspI AAT'ATT Cuts at: 5 52 4480 5 Size: 47 4428 2081 Fragments arranged by size: 4428 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6556 StyI C'CwwG_G Cuts at: 513 1065 2004 2616 3384 3995 513 Size: 552 939 612 768 611 3074 Fragments arranged by size: 3074 939 768 612 611 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4583 4600 4753 4938 5383 325 Size: 1255 3003 17 153 185 445 1498 Fragments arranged by size: 3003 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2239 3009 3481 Size: 274 80 33 51 1443 262 770 472 Cuts at: 3481 4802 6514 96 Size: 1321 1712 138 Fragments arranged by size: 1712 1443 1321 770 472 274 262 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2358 2390 2760 2792 6516 6548 2358 Size: 32 370 32 3724 32 2366 Fragments arranged by size: 3724 2366 370 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6556 XbaI T'CTAG_A Cuts at: 3059 3892 3059 Size: 833 5723 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2125 2125 Size: 6556 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6556 XmaI C'CCGG_G Cuts at: 1870 3881 1870 Size: 2011 4545 XmnI GAAnn'nnTTC Cuts at: 3499 3836 4685 3499 Size: 337 849 5370 Fragments arranged by size: 5370 849 337 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4364 276 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI