Restriction map for
pFN21AB5105
(Circular) (Six-base) MAPSORT of: pFN21AB5105.seq Check: 7506 from: 1 to: 6556
pFN21AB5105 6556 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4366 278
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3899 1937
Size: 1962 4594
Acc65I G'GTAC_C
Cuts at: 3877 3877
Size: 6556
AclI AA'CG_TT
Cuts at: 1563 4683 5056 1563
Size: 3120 373 3063
Fragments arranged by size:
3120 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2880 4619 6003 6271 1632
Size: 1248 1739 1384 268 1917
Fragments arranged by size:
1917 1739 1384 1248 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6359
AflIII A'CryG_T
Cuts at: 1203 2373 5455 6432 1203
Size: 1170 3082 977 1327
Fragments arranged by size:
3082 1327 1170 977
AgeI A'CCGG_T
Cuts at: 5387 5387
Size: 6556
AhdI GACnn_n'nnGTC
Cuts at: 5285 5285
Size: 6556
AleI CACnn'nnGTG
Cuts at: 1166 2618 1166
Size: 1452 5104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3392 1184
Size: 2208 4348
AlwNI CAG_nnn'CTG
Cuts at: 2084 3597 4299 5871 2084
Size: 1513 702 1572 2769
Fragments arranged by size:
2769 1572 1513 702
ApaLI G'TGCA_C
Cuts at: 4613 5769 4613
Size: 1156 5400
ApoI r'AATT_y
Cuts at: 1493 2588 3447 3758 3865 4091 4243 6352 1493
Size: 1095 859 311 107 226 152 2109 1697
Fragments arranged by size:
2109 1697 1095 859 311 226 152 107
AseI AT'TA_AT
Cuts at: 160 1032 5110 160
Size: 872 4078 1606
Fragments arranged by size:
4078 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
AvaI C'yCGr_G
Cuts at: 1870 1943 3881 1870
Size: 73 1938 4545
Fragments arranged by size:
4545 1938 73
BamHI G'GATC_C
Cuts at: 3766 3886 4253 3766
Size: 120 367 6069
Fragments arranged by size:
6069 367 120
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3140 3877 5332 618
Size: 325 217 627 1353 737 1455 1842
Fragments arranged by size:
1842 1455 1353 737 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2886 3875 1391
Size: 1495 989 4072
Fragments arranged by size:
4072 1495 989
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3471 3828 928
Size: 971 1572 357 3656
Fragments arranged by size:
3656 1572 971 357
BbvCI CC'TCA_GC
Cuts at: 3069 3069
Size: 6556
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4747 4781 1903
Size: 34 2810 34 3678
Fragments arranged by size:
3678 2810 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4448 5658 4448
Size: 1210 5346
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6556
BfrBI ATG'CAT
Cuts at: 3836 3836
Size: 6556
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3149 5167 136
Size: 107 122 71 631 605 1477 2018 1525
Fragments arranged by size:
2018 1525 1477 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6551 6551
Size: 6556
BlpI GC'TnA_GC
Cuts at: 3973 3973
Size: 6556
Bme1580I G_kGCm'C
Cuts at: 1790 3145 4617 5773 1790
Size: 1355 1472 1156 2573
Fragments arranged by size:
2573 1472 1355 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6556
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4287 5245 442
Size: 416 53 83 399 24 2870 958 1753
Fragments arranged by size:
2870 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6556
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3752
Size: 6556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2772 3551 5216 1239
Size: 1533 779 1665 2579
Fragments arranged by size:
2579 1665 1533 779
Bpu10I CC'TnA_GC
Cuts at: 3069 3441 3069
Size: 372 6184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4681 5546 5844 4681
Size: 865 298 5393
Fragments arranged by size:
5393 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2080 5219 882
Size: 617 581 3139 2219
Fragments arranged by size:
3139 2219 617 581
BsaAI yAC'GTr
Cuts at: 493 1204 4347 493
Size: 711 3143 2702
Fragments arranged by size:
3143 2702 711
BsaBI GATnn'nnATC
Cuts at: 6550 6550
Size: 6556
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4363 4745 5380 275
Size: 53 83 186 936 2830 382 635 1451
Fragments arranged by size:
2830 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4989 5387 5661 5808 6219 4989
Size: 398 274 147 411 5326
Fragments arranged by size:
5326 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2223 2253 2358 2388 1178
Size: 30 165 30 820 30 105 30 5346
Fragments arranged by size:
5346 820 165 105 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2379 1186
Size: 1193 5363
BseYI C'CCAG_C
Cuts at: 5759 6255 5759
Size: 496 6060
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2133 1163
Size: 573 397 5586
Fragments arranged by size:
5586 573 397
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4006 4767 5795 1540
Size: 275 186 2005 761 1028 2301
Fragments arranged by size:
2301 2005 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2886 3723 4617 4702 5773 2886
Size: 837 894 85 1071 3669
Fragments arranged by size:
3669 1071 894 837 85
BsmI GAATG_Cn'
Cuts at: 3836 3838 4067 4160 3836
Size: 2 229 93 6232
Fragments arranged by size:
6232 229 93 2
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2886 3145 3723 3875 4617 4702 5773
Size: 399 1096 259 578 152 742 85 1071
Cuts at: 5773 1391
Size: 2174
Fragments arranged by size:
2174 1096 1071 742 578 399 259 152
85
BspEI T'CCGG_A
Cuts at: 6219 6219
Size: 6556
BspHI T'CATG_A
Cuts at: 4445 6175 4445
Size: 1730 4826
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3911 844
Size: 3067 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4443 1394
Size: 429 2620 3507
Fragments arranged by size:
3507 2620 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2538 5051 5225 65
Size: 1183 1290 2513 174 1396
Fragments arranged by size:
2513 1396 1290 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5200 5387 1700
Size: 110 3390 187 2869
Fragments arranged by size:
3390 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3481 96
Size: 3385 3171
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6556
BssSI C'ACGA_G
Cuts at: 4616 5628 4616
Size: 1012 5544
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6556
BstBI TT'CG_AA
Cuts at: 5360 5360
Size: 6556
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6556
BstYI r'GATC_y
Cuts at: 1972 2233 3766 3886 4253 4638 4655 6107 6193
Size: 261 1533 120 367 385 17 1452 86
Cuts at: 6193 6205 6551 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1533 1452 385 367 346 261 120
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2616 513
Size: 552 939 612 4453
Fragments arranged by size:
4453 939 612 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5817
Fragments arranged by size:
5817 683 56
BtsI GCAGTG_nn'
Cuts at: 3675 4072 4866 4886 3675
Size: 397 794 20 5345
Fragments arranged by size:
5345 794 397 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6556
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6521
DraI TTT'AAA
Cuts at: 3860 3860
Size: 6556
DraIII CAC_nnn'GTG
Cuts at: 3245 3245
Size: 6556
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5563 809
Size: 507 4247 1802
Fragments arranged by size:
4247 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2692 4003 4892
Size: 54 1425 50 275 18 862 1311 889
Cuts at: 4892 6521 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1311 889 862 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4003 1537
Size: 275 2191 4090
Fragments arranged by size:
4090 2191 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2918 4486 1353
Size: 1565 1568 3423
Fragments arranged by size:
3423 1568 1565
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2466 5143 5517 5663 387
Size: 1064 138 877 2677 374 146 1280
Fragments arranged by size:
2677 1280 1064 877 374 146 138
EcoICRI GAG'CTC
Cuts at: 2884 2884
Size: 6556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2772 2880 3551 4619 5216 6003 6271
Size: 393 1140 108 671 1068 597 787 268
Cuts at: 6271 1239
Size: 1524
Fragments arranged by size:
1524 1140 1068 787 671 597 393 268
108
EcoO109I rG'GnC_Cy
Cuts at: 2081 3073 2081
Size: 992 5564
EcoRI G'AATT_C
Cuts at: 3865 4243 3865
Size: 378 6178
EcoRV GAT'ATC
Cuts at: 2329 2395 2781 3125 2329
Size: 66 386 344 5760
Fragments arranged by size:
5760 386 344 66
FspI TGC'GCA
Cuts at: 5062 5062
Size: 6556
HaeII r_GCGC'y
Cuts at: 1678 5703 1678
Size: 4025 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3041 3073 5197 5229 5271
Size: 32 135 32 1469 32 2124 32 42
Cuts at: 5271 5303 1373
Size: 32 2626
Fragments arranged by size:
2626 2124 1469 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2688 3900 4146 669
Size: 988 281 750 1212 246 3079
Fragments arranged by size:
3079 1212 988 750 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6556
HpaI GTT'AAC
Cuts at: 4146 4146
Size: 6556
KpnI G_GTAC'C
Cuts at: 3881 3881
Size: 6556
MfeI C'AATT_G
Cuts at: 3349 4155 3349
Size: 806 5750
MluI A'CGCG_T
Cuts at: 6432 6432
Size: 6556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2137 2209 2304 3654 5670 5854 1305
Size: 832 72 95 1350 2016 184 2007
Fragments arranged by size:
2016 2007 1350 832 184 95 72
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2694 10
Size: 54 1425 343 862 3872
Fragments arranged by size:
3872 1425 862 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2618 4514 4873 5032 518
Size: 648 1452 1896 359 159 2042
Fragments arranged by size:
2042 1896 1452 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3972 4649 5797 6042 1606
Size: 2366 677 1148 245 2120
Fragments arranged by size:
2366 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2616 513
Size: 552 939 612 4453
Fragments arranged by size:
4453 939 612 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6556
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6556
NotI GC'GGCC_GC
Cuts at: 4003 4003
Size: 6556
NsiI A_TGCA'T
Cuts at: 3838 3838
Size: 6556
NspI r_CATG'y
Cuts at: 1132 1229 3829 3914 5459 1132
Size: 97 2600 85 1545 2229
Fragments arranged by size:
2600 2229 1545 97 85
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6556
PciI A'CATG_T
Cuts at: 5455 5455
Size: 6556
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3245 1108
Size: 2137 4419
PfoI T'CCnGG_A
Cuts at: 2868 2868
Size: 6556
PmeI GTTT'AAAC
Cuts at: 3860 3860
Size: 6556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4625 4657 6138 6170 4625
Size: 32 1481 32 5011
Fragments arranged by size:
5011 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2081 2081
Size: 6556
PshAI GACnn'nnGTC
Cuts at: 1613 2320 1613
Size: 707 5849
PsiI TTA'TAA
Cuts at: 4126 4126
Size: 6556
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2628 2660 2628
Size: 32 6524
PstI C_TGCA'G
Cuts at: 830 3115 3456 3908 830
Size: 2285 341 452 3478
Fragments arranged by size:
3478 2285 452 341
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6556
SacI G_AGCT'C
Cuts at: 2886 2886
Size: 6556
SalI G'TCGA_C
Cuts at: 1936 3898 1936
Size: 1962 4594
SbfI CC_TGCA'GG
Cuts at: 3908 3908
Size: 6556
ScaI AGT'ACT
Cuts at: 3011 4804 6516 3011
Size: 1793 1712 3051
Fragments arranged by size:
3051 1793 1712
SexAI A'CCwGG_T
Cuts at: 3241 3241
Size: 6556
SfcI C'TryA_G
Cuts at: 826 1045 2265 3111 3364 3452 3904 5039 5720
Size: 219 1220 846 253 88 452 1135 681
Cuts at: 5720 5911 826
Size: 191 1471
Fragments arranged by size:
1471 1220 1135 846 681 452 253 219
191 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6556
SmaI CCC'GGG
Cuts at: 1872 3883 1872
Size: 2011 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4660 5561 5823 820
Size: 197 926 2717 901 262 1553
Fragments arranged by size:
2717 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4347 493
Size: 3854 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6556
SphI G_CATG'C
Cuts at: 1132 3914 1132
Size: 2782 3774
SspI AAT'ATT
Cuts at: 5 52 4480 5
Size: 47 4428 2081
Fragments arranged by size:
4428 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6556
StyI C'CwwG_G
Cuts at: 513 1065 2004 2616 3384 3995 513
Size: 552 939 612 768 611 3074
Fragments arranged by size:
3074 939 768 612 611 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4583 4600 4753 4938 5383 325
Size: 1255 3003 17 153 185 445 1498
Fragments arranged by size:
3003 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2239 3009 3481
Size: 274 80 33 51 1443 262 770 472
Cuts at: 3481 4802 6514 96
Size: 1321 1712 138
Fragments arranged by size:
1712 1443 1321 770 472 274 262 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2358 2390 2760 2792 6516 6548 2358
Size: 32 370 32 3724 32 2366
Fragments arranged by size:
3724 2366 370 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6556
XbaI T'CTAG_A
Cuts at: 3059 3892 3059
Size: 833 5723
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2125 2125
Size: 6556
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6556
XmaI C'CCGG_G
Cuts at: 1870 3881 1870
Size: 2011 4545
XmnI GAAnn'nnTTC
Cuts at: 3499 3836 4685 3499
Size: 337 849 5370
Fragments arranged by size:
5370 849 337
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4364 276
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PvuII RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI