Restriction map for
pFN21AB5103
(Circular) (Six-base) MAPSORT of: pFN21AB5103.seq Check: 5895 from: 1 to: 6805
pFN21AB5103 6805 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4615 278
Size: 53 83 186 936 3079 2468
Fragments arranged by size:
3079 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3492 4148 1937
Size: 1555 656 4594
Fragments arranged by size:
4594 1555 656
Acc65I G'GTAC_C
Cuts at: 4126 4126
Size: 6805
AclI AA'CG_TT
Cuts at: 1563 4932 5305 1563
Size: 3369 373 3063
Fragments arranged by size:
3369 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 2793 3804 4868 6252 6520 1632
Size: 398 763 1011 1064 1384 268 1917
Fragments arranged by size:
1917 1384 1064 1011 763 398 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6608
AflIII A'CryG_T
Cuts at: 1203 3204 3531 5704 6681 1203
Size: 2001 327 2173 977 1327
Fragments arranged by size:
2173 2001 1327 977 327
AgeI A'CCGG_T
Cuts at: 5636 5636
Size: 6805
AhdI GACnn_n'nnGTC
Cuts at: 5534 5534
Size: 6805
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6805
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6805
AlwNI CAG_nnn'CTG
Cuts at: 3814 3837 4548 6120 3814
Size: 23 711 1572 4499
Fragments arranged by size:
4499 1572 711 23
ApaLI G'TGCA_C
Cuts at: 2340 4862 6018 2340
Size: 2522 1156 3127
Fragments arranged by size:
3127 2522 1156
ApoI r'AATT_y
Cuts at: 1493 2444 2781 3396 3449 3553 4114 4340 4492
Size: 951 337 615 53 104 561 226 152
Cuts at: 4492 6601 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 951 615 561 337 226 152
104 53
AseI AT'TA_AT
Cuts at: 160 1032 5359 160
Size: 872 4327 1606
Fragments arranged by size:
4327 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6805
AvaI C'yCGr_G
Cuts at: 1870 1943 2257 4130 1870
Size: 73 314 1873 4545
Fragments arranged by size:
4545 1873 314 73
BamHI G'GATC_C
Cuts at: 4135 4502 4135
Size: 367 6438
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2118 2268 2696 4126 5581
Size: 325 217 627 331 150 428 1430 1455
Cuts at: 5581 618
Size: 1842
Fragments arranged by size:
1842 1455 1430 627 428 331 325 217
150
BanII G_rGCy'C
Cuts at: 1391 3291 4124 1391
Size: 1900 833 4072
Fragments arranged by size:
4072 1900 833
BbeI G_GCGC'C
Cuts at: 2272 2272
Size: 6805
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5834
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3260 3294 4996 5030 1903
Size: 34 50 34 1239 34 1702 34 3678
Fragments arranged by size:
3678 1702 1239 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2992 4697 5907 2992
Size: 1705 1210 3890
Fragments arranged by size:
3890 1705 1210
BclI T'GATC_A
Cuts at: 1548 4051 1548
Size: 2503 4302
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3090 5416 136
Size: 107 122 71 631 605 1418 2326 1525
Fragments arranged by size:
2326 1525 1418 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6800 6800
Size: 6805
BlpI GC'TnA_GC
Cuts at: 2858 4222 2858
Size: 1364 5441
Bme1580I G_kGCm'C
Cuts at: 1790 2344 2701 4866 6022 1790
Size: 554 357 2165 1156 2573
Fragments arranged by size:
2573 2165 1156 554 357
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6805
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4536 5494 442
Size: 416 53 83 399 24 3119 958 1753
Fragments arranged by size:
3119 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6805
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2294 2345 3551 3814 5465 1239
Size: 962 93 51 1206 263 1651 2579
Fragments arranged by size:
2579 1651 1206 962 263 93 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4930 5795 6093 4930
Size: 865 298 5642
Fragments arranged by size:
5642 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2397 5468 882
Size: 617 898 3071 2219
Fragments arranged by size:
3071 2219 898 617
BsaAI yAC'GTr
Cuts at: 493 1204 3532 4596 493
Size: 711 2328 1064 2702
Fragments arranged by size:
2702 2328 1064 711
BsaBI GATnn'nnATC
Cuts at: 3321 6799 3321
Size: 3478 3327
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2269 4612 4994 5629
Size: 53 83 186 936 736 2343 382 635
Cuts at: 5629 275
Size: 1451
Fragments arranged by size:
2343 1451 936 736 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3647 5238 5636 5910 6057 6468 3647
Size: 1591 398 274 147 411 3984
Fragments arranged by size:
3984 1591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6580
Fragments arranged by size:
6580 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2125 2853 1186
Size: 939 728 5138
Fragments arranged by size:
5138 939 728
BseYI C'CCAG_C
Cuts at: 2175 3888 6008 6504 2175
Size: 1713 2120 496 2476
Fragments arranged by size:
2476 2120 1713 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3789 3838 1163
Size: 573 2053 49 4130
Fragments arranged by size:
4130 2053 573 49
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4255 5016 6044 1540
Size: 275 186 2254 761 1028 2301
Fragments arranged by size:
2301 2254 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2344 3830 4866 4951 6022 2344
Size: 1486 1036 85 1071 3127
Fragments arranged by size:
3127 1486 1071 1036 85
BsmI GAATG_Cn'
Cuts at: 2867 3788 4316 4409 2867
Size: 921 528 93 5263
Fragments arranged by size:
5263 921 528 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2732 2732
Size: 6805
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2344 2701 3291 3830 4124 4866 4951
Size: 399 554 357 590 539 294 742 85
Cuts at: 4951 6022 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 590 554 539 399 357
294 85
BspEI T'CCGG_A
Cuts at: 6468 6468
Size: 6805
BspHI T'CATG_A
Cuts at: 2947 4694 6424 2947
Size: 1747 1730 3328
Fragments arranged by size:
3328 1747 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3811 4031 4160 844
Size: 2967 220 129 3489
Fragments arranged by size:
3489 2967 220 129
BsrBI CCG'CTC
Cuts at: 1394 1823 2100 4692 1394
Size: 429 277 2592 3507
Fragments arranged by size:
3507 2592 429 277
BsrDI GCAATG_nn'
Cuts at: 65 1248 5300 5474 65
Size: 1183 4052 174 1396
Fragments arranged by size:
4052 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5449 5636 1700
Size: 110 3639 187 2869
Fragments arranged by size:
3639 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3099 96
Size: 3003 3802
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6805
BssSI C'ACGA_G
Cuts at: 4865 5877 4865
Size: 1012 5793
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6805
BstBI TT'CG_AA
Cuts at: 5609 5609
Size: 6805
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6805
BstYI r'GATC_y
Cuts at: 1972 3754 4084 4135 4502 4887 4904 6356 6442
Size: 1782 330 51 367 385 17 1452 86
Cuts at: 6442 6454 6800 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1782 1452 385 367 346 330 86
51 17 12
BstZ17I GTA'TAC
Cuts at: 3493 3493
Size: 6805
Bsu36I CC'TnA_GG
Cuts at: 3777 3777
Size: 6805
BtgI C'CryG_G
Cuts at: 513 1065 2043 3792 3861 513
Size: 552 978 1749 69 3457
Fragments arranged by size:
3457 1749 978 552 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6066
Fragments arranged by size:
6066 683 56
BtsI GCAGTG_nn'
Cuts at: 3596 4321 5115 5135 3596
Size: 725 794 20 5266
Fragments arranged by size:
5266 794 725 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6805
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2912 2947 529
Size: 35 2348 35 4387
Fragments arranged by size:
4387 2348 35 35
DraI TTT'AAA
Cuts at: 4109 4109
Size: 6805
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5812 809
Size: 507 4496 1802
Fragments arranged by size:
4496 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 4252 5141
Size: 54 1425 50 275 18 210 2212 889
Cuts at: 5141 6770 8
Size: 1629 43
Fragments arranged by size:
2212 1629 1425 889 275 210 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4252 1537
Size: 275 2440 4090
Fragments arranged by size:
4090 2440 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2616 2735 4735 1353
Size: 1263 119 2000 3423
Fragments arranged by size:
3423 2000 1263 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5392 5766 5912 387
Size: 1064 138 3803 374 146 1280
Fragments arranged by size:
3803 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2201 2294 2345 2793 3551 3804
Size: 393 398 171 93 51 448 758 253
Cuts at: 3804 3814 4868 5465 6252 6520 1239
Size: 10 1054 597 787 268 1524
Fragments arranged by size:
1524 1054 787 758 597 448 398 393
268 253 171 93 51 10
EcoO109I rG'GnC_Cy
Cuts at: 3627 3627
Size: 6805
EcoRI G'AATT_C
Cuts at: 2444 3396 3449 4114 4492 2444
Size: 952 53 665 378 4757
Fragments arranged by size:
4757 952 665 378 53
EcoRV GAT'ATC
Cuts at: 3986 3986
Size: 6805
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4004 4004
Size: 6805
FspI TGC'GCA
Cuts at: 5311 5311
Size: 6805
HaeII r_GCGC'y
Cuts at: 1678 2146 2272 5952 1678
Size: 468 126 3680 2531
Fragments arranged by size:
3680 2531 468 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3060 3092 3709 3741 4055
Size: 32 135 32 1488 32 617 32 314
Cuts at: 4055 4087 5446 5478 5520 5552 1373
Size: 32 1359 32 42 32 2626
Fragments arranged by size:
2626 1488 1359 617 314 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4149 4395 669
Size: 988 281 2211 246 3079
Fragments arranged by size:
3079 2211 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6805
HpaI GTT'AAC
Cuts at: 4395 4395
Size: 6805
KasI G'GCGC_C
Cuts at: 2268 2268
Size: 6805
KpnI G_GTAC'C
Cuts at: 4130 4130
Size: 6805
MfeI C'AATT_G
Cuts at: 4404 4404
Size: 6805
MluI A'CGCG_T
Cuts at: 6681 6681
Size: 6805
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5919 6103 1305
Size: 4614 184 2007
Fragments arranged by size:
4614 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4983
Fragments arranged by size:
4983 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3203 3942 4064 4763 5122 5281 518
Size: 648 2037 739 122 699 359 159 2042
Fragments arranged by size:
2042 2037 739 699 648 359 159 122
MspA1I CmG'CkG
Cuts at: 1606 2179 4221 4898 6046 6291 1606
Size: 573 2042 677 1148 245 2120
Fragments arranged by size:
2120 2042 1148 677 573 245
NarI GG'CG_CC
Cuts at: 2269 2269
Size: 6805
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6253
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6805
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6805
NotI GC'GGCC_GC
Cuts at: 4252 4252
Size: 6805
NspI r_CATG'y
Cuts at: 1132 1229 3208 3933 4163 5708 1132
Size: 97 1979 725 230 1545 2229
Fragments arranged by size:
2229 1979 1545 725 230 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6805
PciI A'CATG_T
Cuts at: 3204 5704 3204
Size: 2500 4305
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6805
PmeI GTTT'AAAC
Cuts at: 4109 4109
Size: 6805
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4874 4906 6387 6419 4874
Size: 32 1481 32 5260
Fragments arranged by size:
5260 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3627 3627
Size: 6805
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6805
PsiI TTA'TAA
Cuts at: 4375 4375
Size: 6805
PstI C_TGCA'G
Cuts at: 830 3881 3908 4157 830
Size: 3051 27 249 3478
Fragments arranged by size:
3478 3051 249 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6805
PvuII CAG'CTG
Cuts at: 2179 2179
Size: 6805
SalI G'TCGA_C
Cuts at: 1936 4147 1936
Size: 2211 4594
SbfI CC_TGCA'GG
Cuts at: 4157 4157
Size: 6805
ScaI AGT'ACT
Cuts at: 2412 5053 6765 2412
Size: 2641 1712 2452
Fragments arranged by size:
2641 2452 1712
SfcI C'TryA_G
Cuts at: 826 1045 3401 3631 3877 3904 4153 5288 5969
Size: 219 2356 230 246 27 249 1135 681
Cuts at: 5969 6160 826
Size: 191 1471
Fragments arranged by size:
2356 1471 1135 681 249 246 230 219
191 27
SfoI GGC'GCC
Cuts at: 2270 2270
Size: 6805
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6805
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6805
SmaI CCC'GGG
Cuts at: 1872 2259 4132 1872
Size: 387 1873 4545
Fragments arranged by size:
4545 1873 387
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4909 5810 6072 820
Size: 197 926 2966 901 262 1553
Fragments arranged by size:
2966 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4596 493
Size: 4103 2702
SpeI A'CTAG_T
Cuts at: 152 3620 152
Size: 3468 3337
SphI G_CATG'C
Cuts at: 1132 4163 1132
Size: 3031 3774
SspI AAT'ATT
Cuts at: 5 52 4729 5
Size: 47 4677 2081
Fragments arranged by size:
4677 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6805
StyI C'CwwG_G
Cuts at: 513 1065 3230 4244 513
Size: 552 2165 1014 3074
Fragments arranged by size:
3074 2165 1014 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3973 4832 4849 5002 5187 5632 325
Size: 1255 2393 859 17 153 185 445 1498
Fragments arranged by size:
2393 1498 1255 859 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2410 3099 5051
Size: 274 80 33 51 1443 433 689 1952
Cuts at: 5051 6763 96
Size: 1712 138
Fragments arranged by size:
1952 1712 1443 689 433 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3516 3548 6765 6797 3516
Size: 32 3217 32 3524
Fragments arranged by size:
3524 3217 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6805
XbaI T'CTAG_A
Cuts at: 4141 4141
Size: 6805
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3728 3728
Size: 6805
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6805
XmaI C'CCGG_G
Cuts at: 1870 2257 4130 1870
Size: 387 1873 4545
Fragments arranged by size:
4545 1873 387
XmnI GAAnn'nnTTC
Cuts at: 4934 4934
Size: 6805
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4613 276
Size: 53 83 186 936 3079 2468
Fragments arranged by size:
3079 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI DraIII EcoICRI EcoNI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI