Restriction map for
pFN21AB5098
(Circular) (Six-base) MAPSORT of: pFN21AB5098.seq Check: 4635 from: 1 to: 8179
pFN21AB5098 8179 bp
With 182 enzymes: SgfI *
March 28, 2012 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4702 4702
Size: 8179
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5989 278
Size: 53 83 186 936 4453 2468
Fragments arranged by size:
4453 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3639 5522 1937
Size: 1702 1883 4594
Fragments arranged by size:
4594 1883 1702
Acc65I G'GTAC_C
Cuts at: 5500 5500
Size: 8179
AclI AA'CG_TT
Cuts at: 1563 6306 6679 1563
Size: 4743 373 3063
Fragments arranged by size:
4743 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3041 3421 4570 6242 7626 7894 1632
Size: 1409 380 1149 1672 1384 268 1917
Fragments arranged by size:
1917 1672 1409 1384 1149 380 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7982
AflIII A'CryG_T
Cuts at: 1203 3958 7078 8055 1203
Size: 2755 3120 977 1327
Fragments arranged by size:
3120 2755 1327 977
AgeI A'CCGG_T
Cuts at: 3266 7010 3266
Size: 3744 4435
AhdI GACnn_n'nnGTC
Cuts at: 6908 6908
Size: 8179
AleI CACnn'nnGTG
Cuts at: 1166 3521 5267 1166
Size: 2355 1746 4078
Fragments arranged by size:
4078 2355 1746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3285 3905 3989 1184
Size: 2101 620 84 5374
Fragments arranged by size:
5374 2101 620 84
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3839 3871 3839
Size: 32 8147
AlwNI CAG_nnn'CTG
Cuts at: 3928 4357 5922 7494 3928
Size: 429 1565 1572 4613
Fragments arranged by size:
4613 1572 1565 429
ApaLI G'TGCA_C
Cuts at: 4715 6236 7392 4715
Size: 1521 1156 5502
Fragments arranged by size:
5502 1521 1156
ApoI r'AATT_y
Cuts at: 1493 5488 5714 5866 7975 1493
Size: 3995 226 152 2109 1697
Fragments arranged by size:
3995 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4794 6733 160
Size: 872 3762 1939 1606
Fragments arranged by size:
3762 1939 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8179
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 2151 2183 2576 2801 3851 4380
Size: 73 174 34 32 393 225 1050 529
Cuts at: 4380 4846 4888 5300 5324 5372 5504 1870
Size: 466 42 412 24 48 132 4545
Fragments arranged by size:
4545 1050 529 466 412 393 225 174
132 73 48 42 34 32 24
AvrII C'CTAG_G
Cuts at: 5134 5134
Size: 8179
BamHI G'GATC_C
Cuts at: 3873 5509 5876 3873
Size: 1636 367 6176
Fragments arranged by size:
6176 1636 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2023 2073 2317 2602 2757
Size: 325 217 627 236 50 244 285 155
Cuts at: 2757 3269 3693 3997 5112 5500 6955 618
Size: 512 424 304 1115 388 1455 1842
Fragments arranged by size:
1842 1455 1115 627 512 424 388 325
304 285 244 236 217 155 50
BanII G_rGCy'C
Cuts at: 1391 2151 2190 2823 3105 3537 3785 3852 4046
Size: 760 39 633 282 432 248 67 194
Cuts at: 4046 4491 4617 5205 5301 5325 5498 1391
Size: 445 126 588 96 24 173 4072
Fragments arranged by size:
4072 760 633 588 445 432 282 248
194 173 126 96 67 39 24
BbeI G_GCGC'C
Cuts at: 2027 2077 2606 2761 5116 2027
Size: 50 529 155 2355 5090
Fragments arranged by size:
5090 2355 529 155 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4814 928
Size: 971 2915 4293
Fragments arranged by size:
4293 2915 971
BbvCI CC'TCA_GC
Cuts at: 4140 4140
Size: 8179
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3299 3333 4519 4553 6370 6404 1903
Size: 34 1362 34 1186 34 1817 34 3678
Fragments arranged by size:
3678 1817 1362 1186 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4234 4321 6071 7281 4234
Size: 87 1750 1210 5132
Fragments arranged by size:
5132 1750 1210 87
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8179
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 6790 136
Size: 107 122 71 631 605 337 4781 1525
Fragments arranged by size:
4781 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3798 3813 8174 3798
Size: 15 4361 3803
Fragments arranged by size:
4361 3803 15
BlpI GC'TnA_GC
Cuts at: 2033 2988 5596 2033
Size: 955 2608 4616
Fragments arranged by size:
4616 2608 955
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2322 3241 3749 4002 4719 5098 6240
Size: 276 256 919 508 253 717 379 1142
Cuts at: 6240 7396 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1142 919 717 508 379 276
256 253
BmgBI CAC'GTC
Cuts at: 1318 2164 1318
Size: 846 7333
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3322 3851 4069
Size: 416 53 83 399 24 1905 529 218
Cuts at: 4069 5332 5910 6868 442
Size: 1263 578 958 1753
Fragments arranged by size:
1905 1753 1263 958 578 529 416 399
218 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2088 2561 3379 3629 4407 4631 6839 1239
Size: 849 473 818 250 778 224 2208 2579
Fragments arranged by size:
2579 2208 849 818 778 473 250 224
Bpu10I CC'TnA_GC
Cuts at: 2145 2670 4140 5186 2145
Size: 525 1470 1046 5138
Fragments arranged by size:
5138 1470 1046 525
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3779 6304 7169 7467 3779
Size: 2525 865 298 4491
Fragments arranged by size:
4491 2525 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2633 2678 3053 4408 5249 5327 5351
Size: 617 1134 45 375 1355 841 78 24
Cuts at: 5351 5444 6842 882
Size: 93 1398 2219
Fragments arranged by size:
2219 1398 1355 1134 841 617 375 93
78 45 24
BsaAI yAC'GTr
Cuts at: 493 1204 2923 3959 5970 493
Size: 711 1719 1036 2011 2702
Fragments arranged by size:
2702 2011 1719 1036 711
BsaBI GATnn'nnATC
Cuts at: 8173 8173
Size: 8179
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2024 2074 2603 2758
Size: 53 83 186 936 491 50 529 155
Cuts at: 2758 3137 3372 5053 5068 5113 5986 6368 7003
Size: 379 235 1681 15 45 873 382 635
Cuts at: 7003 275
Size: 1451
Fragments arranged by size:
1681 1451 936 873 635 529 491 382
379 235 186 155 83 53 50 45
15
BsaWI w'CCGG_w
Cuts at: 2263 3266 4440 6612 7010 7284 7431 7842 2263
Size: 1003 1174 2172 398 274 147 411 2600
Fragments arranged by size:
2600 2172 1174 1003 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3116 3146 3839 3869 4388
Size: 30 165 30 1713 30 693 30 519
Cuts at: 4388 4418 5137 5167 5288 5312 5318 5336 5342
Size: 30 719 30 121 24 6 18 6
Cuts at: 5342 5366 1178
Size: 24 3991
Fragments arranged by size:
3991 1713 719 693 519 165 121 30
30 30 30 30 30 24 24 18
6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2594 2651 2693 2696 2809 2843 2870
Size: 937 471 57 42 3 113 34 27
Cuts at: 2870 2955 3029 3071 3131 3140 3143 3202 3755
Size: 85 74 42 60 9 3 59 553
Cuts at: 3755 4097 4157 5402 5405 5408 5411 5420 5423
Size: 342 60 1245 3 3 3 9 3
Cuts at: 5423 1186
Size: 3942
Fragments arranged by size:
3942 1245 937 553 471 342 113 85
74 60 60 59 57 42 42 34
27 9 9 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 2564 2845 3616 3869 3892 4066 4276 5235 5361
Size: 281 771 253 23 174 210 959 126
Cuts at: 5361 7382 7878 2564
Size: 2021 496 2865
Fragments arranged by size:
2865 2021 959 771 496 281 253 210
174 126 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3995 1163
Size: 573 2259 5347
Fragments arranged by size:
5347 2259 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 2250 3250 5629 6390 7418
Size: 275 186 12 237 1000 2379 761 1028
Cuts at: 7418 1540
Size: 2301
Fragments arranged by size:
2379 2301 1028 1000 761 275 237 186
12
BsiHKAI G_wGCw'C
Cuts at: 2664 3537 3626 3785 4131 4617 4719 5192 6240
Size: 873 89 159 346 486 102 473 1048
Cuts at: 6240 6325 7396 2664
Size: 85 1071 3447
Fragments arranged by size:
3447 1071 1048 873 486 473 346 159
102 89 85
BsmI GAATG_Cn'
Cuts at: 5429 5690 5783 5429
Size: 261 93 7825
Fragments arranged by size:
7825 261 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2151 2190 2322 2664 2823 3105
Size: 399 276 85 39 132 342 159 282
Cuts at: 3105 3241 3537 3626 3749 3785 3852 4002 4046
Size: 136 296 89 123 36 67 150 44
Cuts at: 4046 4131 4491 4617 4719 5098 5192 5205 5301
Size: 85 360 126 102 379 94 13 96
Cuts at: 5301 5325 5498 6240 6325 7396 1391
Size: 24 173 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 360 342 296
282 276 173 159 150 136 132 126
123 102 96 94 89 85 85 85
67 44 39 36 24 13
BspEI T'CCGG_A
Cuts at: 2263 7842 2263
Size: 5579 2600
BspHI T'CATG_A
Cuts at: 3723 4648 6068 7798 3723
Size: 925 1420 1730 4104
Fragments arranged by size:
4104 1730 1420 925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4602 4702 4910 5534 844
Size: 3758 100 208 624 3489
Fragments arranged by size:
3758 3489 624 208 100
BsrBI CCG'CTC
Cuts at: 1394 1823 2806 4303 6066 1394
Size: 429 983 1497 1763 3507
Fragments arranged by size:
3507 1763 1497 983 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3448 4245 4761 4912 6674 6848 65
Size: 1183 2200 797 516 151 1762 174 1396
Fragments arranged by size:
2200 1762 1396 1183 797 516 174 151
BsrFI r'CCGG_y
Cuts at: 1700 1810 3249 3266 3544 4523 5001 6823 7010
Size: 110 1439 17 278 979 478 1822 187
Cuts at: 7010 1700
Size: 2869
Fragments arranged by size:
2869 1822 1439 979 478 278 187 110
17
BsrGI T'GTAC_A
Cuts at: 96 3465 96
Size: 3369 4810
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8179
BssSI C'ACGA_G
Cuts at: 3786 6239 7251 3786
Size: 2453 1012 4714
Fragments arranged by size:
4714 2453 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2086 3597 794
Size: 1292 1511 5376
Fragments arranged by size:
5376 1511 1292
BstBI TT'CG_AA
Cuts at: 6983 6983
Size: 8179
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8179
BstXI CCAn_nnnn'nTGG
Cuts at: 3172 3582 4609 3172
Size: 410 1027 6742
Fragments arranged by size:
6742 1027 410
BstYI r'GATC_y
Cuts at: 1972 3201 3798 3813 3873 4114 5509 5876 6261
Size: 1229 597 15 60 241 1395 367 385
Cuts at: 6261 6278 7730 7816 7828 8174 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1395 1229 597 385 367 346
241 86 60 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 2412 2826 2412
Size: 414 7765
BtgI C'CryG_G
Cuts at: 513 1065 2004 2195 3226 3807 3877 4218 4227
Size: 552 939 191 1031 581 70 341 9
Cuts at: 4227 4368 4495 4720 513
Size: 141 127 225 3972
Fragments arranged by size:
3972 1031 939 581 552 341 225 191
141 127 70 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3960 5010 487
Size: 683 56 2734 1050 3656
Fragments arranged by size:
3656 2734 1050 683 56
BtsI GCAGTG_nn'
Cuts at: 2549 3081 3864 5695 6489 6509 2549
Size: 532 783 1831 794 20 4219
Fragments arranged by size:
4219 1831 794 783 532 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8179
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8144
DraI TTT'AAA
Cuts at: 5483 5483
Size: 8179
DraIII CAC_nnn'GTG
Cuts at: 3997 4804 4935 5149 3997
Size: 807 131 214 7027
Fragments arranged by size:
7027 807 214 131
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7186 809
Size: 507 5870 1802
Fragments arranged by size:
5870 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2237 2247
Size: 54 1425 50 275 18 180 227 10
Cuts at: 2247 2546 2813 3247 3669 4359 4986 5422 5626
Size: 299 267 434 422 690 627 436 204
Cuts at: 5626 6515 8144 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 690 627 436 434 422
299 275 267 227 204 180 54 50
43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2010 2247 3247 5626 1537
Size: 275 198 237 1000 2379 4090
Fragments arranged by size:
4090 2379 1000 275 237 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2501 3000 5379 5397 6109 1353
Size: 1148 499 2379 18 712 3423
Fragments arranged by size:
3423 2379 1148 712 499 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2958 6766 7140 7286 387
Size: 1064 138 1369 3808 374 146 1280
Fragments arranged by size:
3808 1369 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3535 3783 4615 3535
Size: 248 832 7099
Fragments arranged by size:
7099 832 248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 2561 3041 3379 3421 3629 4407
Size: 393 456 473 480 338 42 208 778
Cuts at: 4407 4570 4631 6242 6839 7626 7894 1239
Size: 163 61 1611 597 787 268 1524
Fragments arranged by size:
1611 1524 787 778 597 480 473 456
393 338 268 208 163 61 42
EcoNI CCTnn'n_nnAGG
Cuts at: 3974 3974
Size: 8179
EcoO109I rG'GnC_Cy
Cuts at: 2170 2309 2340 2511 2836 3324 4156 4168 5138
Size: 139 31 171 325 488 832 12 970
Cuts at: 5138 5209 2170
Size: 71 5140
Fragments arranged by size:
5140 970 832 488 325 171 139 71
31 12
EcoRI G'AATT_C
Cuts at: 5488 5866 5488
Size: 378 7801
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2347 4285 2347
Size: 1938 6241
FspI TGC'GCA
Cuts at: 3965 6685 3965
Size: 2720 5459
HaeII r_GCGC'y
Cuts at: 1678 2027 2077 2606 2761 2995 4067 4694 4896
Size: 349 50 529 155 234 1072 627 202
Cuts at: 4896 5116 7326 1678
Size: 220 2210 2531
Fragments arranged by size:
2531 2210 1072 627 529 349 234 220
202 155 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2854 2886 4106 4138 4388
Size: 32 135 32 1282 32 1220 32 250
Cuts at: 4388 4420 6820 6852 6894 6926 1373
Size: 32 2400 32 42 32 2626
Fragments arranged by size:
2626 2400 1282 1220 250 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4543 4912 5523 5769 669
Size: 988 281 2605 369 611 246 3079
Fragments arranged by size:
3079 2605 988 611 369 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8179
HpaI GTT'AAC
Cuts at: 5769 5769
Size: 8179
KasI G'GCGC_C
Cuts at: 2023 2073 2602 2757 5112 2023
Size: 50 529 155 2355 5090
Fragments arranged by size:
5090 2355 529 155 50
KpnI G_GTAC'C
Cuts at: 5504 5504
Size: 8179
MfeI C'AATT_G
Cuts at: 5778 5778
Size: 8179
MluI A'CGCG_T
Cuts at: 8055 8055
Size: 8179
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3102 4983 5226 5322 7293 7477 1305
Size: 1797 1881 243 96 1971 184 2007
Fragments arranged by size:
2007 1971 1881 1797 243 184 96
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2548 2815 3671 4988 5424
Size: 54 1425 343 716 267 856 1317 436
Cuts at: 5424 10
Size: 2765
Fragments arranged by size:
2765 1425 1317 856 716 436 343 267
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3170 3521 3728 4653 4703 4781 4988
Size: 648 2004 351 207 925 50 78 207
Cuts at: 4988 5267 6137 6496 6655 518
Size: 279 870 359 159 2042
Fragments arranged by size:
2042 2004 925 870 648 359 351 279
207 207 159 78 50
MspA1I CmG'CkG
Cuts at: 1606 2037 2197 2896 4354 4497 4722 5231 5365
Size: 431 160 699 1458 143 225 509 134
Cuts at: 5365 5595 6272 7420 7665 1606
Size: 230 677 1148 245 2120
Fragments arranged by size:
2120 1458 1148 699 677 509 431 245
230 225 160 143 134
NaeI GCC'GGC
Cuts at: 3251 3251
Size: 8179
NarI GG'CG_CC
Cuts at: 2024 2074 2603 2758 5113 2024
Size: 50 529 155 2355 5090
Fragments arranged by size:
5090 2355 529 155 50
NcoI C'CATG_G
Cuts at: 513 1065 2004 3807 3877 4368 513
Size: 552 939 1803 70 491 4324
Fragments arranged by size:
4324 1803 939 552 491 70
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8179
NgoMIV G'CCGG_C
Cuts at: 3249 3249
Size: 8179
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8179
NotI GC'GGCC_GC
Cuts at: 5626 5626
Size: 8179
NspI r_CATG'y
Cuts at: 1132 1229 3262 3604 3715 5537 7082 1132
Size: 97 2033 342 111 1822 1545 2229
Fragments arranged by size:
2229 2033 1822 1545 342 111 97
PasI CC'CwG_GG
Cuts at: 1348 3382 3827 5205 1348
Size: 2034 445 1378 4322
Fragments arranged by size:
4322 2034 1378 445
PciI A'CATG_T
Cuts at: 7078 7078
Size: 8179
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3232 1108
Size: 2124 6055
PfoI T'CCnGG_A
Cuts at: 2629 4110 4974 2629
Size: 1481 864 5834
Fragments arranged by size:
5834 1481 864
PmeI GTTT'AAAC
Cuts at: 5483 5483
Size: 8179
PmlI CAC'GTG
Cuts at: 3959 3959
Size: 8179
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 6248 6280 7761 7793 3839
Size: 32 2377 32 1481 32 4225
Fragments arranged by size:
4225 2377 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2340 3324 4156 4168 2340
Size: 984 832 12 6351
Fragments arranged by size:
6351 984 832 12
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8179
PsiI TTA'TAA
Cuts at: 5749 5749
Size: 8179
PspXI vC'TCGA_Gb
Cuts at: 2117 2117
Size: 8179
PstI C_TGCA'G
Cuts at: 830 5061 5531 830
Size: 4231 470 3478
Fragments arranged by size:
4231 3478 470
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8179
PvuII CAG'CTG
Cuts at: 2037 4354 5365 2037
Size: 2317 1011 4851
Fragments arranged by size:
4851 2317 1011
RsrII CG'GwC_CG
Cuts at: 4566 4566
Size: 8179
SacI G_AGCT'C
Cuts at: 3537 3785 4617 3537
Size: 248 832 7099
Fragments arranged by size:
7099 832 248
SacII CC_GC'GG
Cuts at: 2198 4498 4723 2198
Size: 2300 225 5654
Fragments arranged by size:
5654 2300 225
SalI G'TCGA_C
Cuts at: 1936 5521 1936
Size: 3585 4594
SanDI GG'GwC_CC
Cuts at: 2340 4156 4168 2340
Size: 1816 12 6351
Fragments arranged by size:
6351 1816 12
SbfI CC_TGCA'GG
Cuts at: 5531 5531
Size: 8179
ScaI AGT'ACT
Cuts at: 6427 8139 6427
Size: 1712 6467
SexAI A'CCwGG_T
Cuts at: 3993 3993
Size: 8179
SfcI C'TryA_G
Cuts at: 826 1045 3640 5057 5527 6662 7343 7534 826
Size: 219 2595 1417 470 1135 681 191 1471
Fragments arranged by size:
2595 1471 1417 1135 681 470 219 191
SfoI GGC'GCC
Cuts at: 2025 2075 2604 2759 5114 2025
Size: 50 529 155 2355 5090
Fragments arranged by size:
5090 2355 529 155 50
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8179
SgrAI Cr'CCGG_yG
Cuts at: 1700 4523 1700
Size: 2823 5356
SmaI CCC'GGG
Cuts at: 1872 2153 4382 4848 5506 1872
Size: 281 2229 466 658 4545
Fragments arranged by size:
4545 2229 658 466 281
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2117 3794 6283 7184 7446 820
Size: 197 926 174 1677 2489 901 262 1553
Fragments arranged by size:
2489 1677 1553 926 901 262 197 174
SnaBI TAC'GTA
Cuts at: 493 5970 493
Size: 5477 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8179
SphI G_CATG'C
Cuts at: 1132 3604 5537 1132
Size: 2472 1933 3774
Fragments arranged by size:
3774 2472 1933
SspI AAT'ATT
Cuts at: 5 52 6103 5
Size: 47 6051 2081
Fragments arranged by size:
6051 2081 47
StuI AGG'CCT
Cuts at: 1517 3792 1517
Size: 2275 5904
StyI C'CwwG_G
Cuts at: 513 1065 2004 2313 2322 2331 2379 2397 2430
Size: 552 939 309 9 9 48 18 33
Cuts at: 2430 2445 2478 3051 3807 3877 4368 5134 5313
Size: 15 33 573 756 70 491 766 179
Cuts at: 5313 5618 513
Size: 305 3074
Fragments arranged by size:
3074 939 766 756 573 552 491 309
305 179 70 48 33 33 18 15
9 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2108 2392 2458 2491 2702 3077 3395
Size: 1255 528 284 66 33 211 375 318
Cuts at: 3395 3448 4499 5303 6206 6223 6376 6561 7006
Size: 53 1051 804 903 17 153 185 445
Cuts at: 7006 325
Size: 1498
Fragments arranged by size:
1498 1255 1051 903 804 528 445 375
318 284 211 185 153 66 53 33
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2748 3465 6425
Size: 274 80 33 51 1443 771 717 2960
Cuts at: 6425 8137 96
Size: 1712 138
Fragments arranged by size:
2960 1712 1443 771 717 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3116 3148 3943 3975 5137 5169 8139 8171 3116
Size: 32 795 32 1162 32 2970 32 3124
Fragments arranged by size:
3124 2970 1162 795 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4400 1316
Size: 3084 5095
XbaI T'CTAG_A
Cuts at: 5515 5515
Size: 8179
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2329 3427 3679 2329
Size: 1098 252 6829
Fragments arranged by size:
6829 1098 252
XhoI C'TCGA_G
Cuts at: 1943 2117 1943
Size: 174 8005
XmaI C'CCGG_G
Cuts at: 1870 2151 4380 4846 5504 1870
Size: 281 2229 466 658 4545
Fragments arranged by size:
4545 2229 658 466 281
XmnI GAAnn'nnTTC
Cuts at: 4268 6308 4268
Size: 2040 6139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5987 276
Size: 53 83 186 936 4453 2468
Fragments arranged by size:
4453 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspOMI
PsrI SapI SfiI SrfI SwaI