Restriction map for
pFN21AB5090
(Circular) (Six-base) MAPSORT of: pFN21AB5090.seq Check: 8890 from: 1 to: 7012
pFN21AB5090 7012 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4822 278
Size: 53 83 186 936 3286 2468
Fragments arranged by size:
3286 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4355 1937
Size: 2418 4594
Acc65I G'GTAC_C
Cuts at: 2043 4333 2043
Size: 2290 4722
AclI AA'CG_TT
Cuts at: 1563 5139 5512 1563
Size: 3576 373 3063
Fragments arranged by size:
3576 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2258 2432 2813 4110 4139 5075 6459 6727
Size: 626 174 381 1297 29 936 1384 268
Cuts at: 6727 1632
Size: 1917
Fragments arranged by size:
1917 1384 1297 936 626 381 268 174
29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6815
AflIII A'CryG_T
Cuts at: 1203 3945 5911 6888 1203
Size: 2742 1966 977 1327
Fragments arranged by size:
2742 1966 1327 977
AgeI A'CCGG_T
Cuts at: 5843 5843
Size: 7012
AhdI GACnn_n'nnGTC
Cuts at: 5741 5741
Size: 7012
AleI CACnn'nnGTG
Cuts at: 1166 2168 3179 3872 1166
Size: 1002 1011 693 4306
Fragments arranged by size:
4306 1011 1002 693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2766 3011 3839 1184
Size: 1582 245 828 4357
Fragments arranged by size:
4357 1582 828 245
AlwNI CAG_nnn'CTG
Cuts at: 2104 2366 2434 2611 3937 4106 4755 6327 2104
Size: 262 68 177 1326 169 649 1572 2789
Fragments arranged by size:
2789 1572 1326 649 262 177 169 68
ApaLI G'TGCA_C
Cuts at: 5069 6225 5069
Size: 1156 5856
ApoI r'AATT_y
Cuts at: 1493 3065 3152 3224 3647 4321 4547 4699 6808
Size: 1572 87 72 423 674 226 152 2109
Cuts at: 6808 1493
Size: 1697
Fragments arranged by size:
2109 1697 1572 674 423 226 152 87
72
AseI AT'TA_AT
Cuts at: 160 1032 5566 160
Size: 872 4534 1606
Fragments arranged by size:
4534 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7012
AvaI C'yCGr_G
Cuts at: 1870 1943 4337 1870
Size: 73 2394 4545
Fragments arranged by size:
4545 2394 73
BamHI G'GATC_C
Cuts at: 3143 4342 4709 3143
Size: 1199 367 5446
Fragments arranged by size:
5446 1199 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2043 2325 3815 4333 5788
Size: 325 217 627 256 282 1490 518 1455
Cuts at: 5788 618
Size: 1842
Fragments arranged by size:
1842 1490 1455 627 518 325 282 256
217
BanII G_rGCy'C
Cuts at: 1391 4331 1391
Size: 2940 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4127 928
Size: 971 2228 3813
Fragments arranged by size:
3813 2228 971
BbvCI CC'TCA_GC
Cuts at: 4240 4240
Size: 7012
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5203 5237 1903
Size: 34 3266 34 3678
Fragments arranged by size:
3678 3266 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4904 6114 4904
Size: 1210 5802
BclI T'GATC_A
Cuts at: 1548 3318 1548
Size: 1770 5242
BfrBI ATG'CAT
Cuts at: 2998 2998
Size: 7012
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2369 5623 136
Size: 107 122 71 631 605 697 3254 1525
Fragments arranged by size:
3254 1525 697 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2563 7007 2563
Size: 4444 2568
BlpI GC'TnA_GC
Cuts at: 4429 4429
Size: 7012
Bme1580I G_kGCm'C
Cuts at: 1790 3170 5073 6229 1790
Size: 1380 1903 1156 2573
Fragments arranged by size:
2573 1903 1380 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7012
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4743 5701 442
Size: 416 53 83 399 24 3326 958 1753
Fragments arranged by size:
3326 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7012
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3391 4303 3391
Size: 912 6100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2456 2492 2876 2939 3795 5672 1239
Size: 830 387 36 384 63 856 1877 2579
Fragments arranged by size:
2579 1877 856 830 387 384 63 36
Bpu10I CC'TnA_GC
Cuts at: 3527 4240 3527
Size: 713 6299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2309 2525 3338 3817 5137 6002 6300 2309
Size: 216 813 479 1320 865 298 3021
Fragments arranged by size:
3021 1320 865 813 479 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3323 3362 5675 882
Size: 617 1824 39 2313 2219
Fragments arranged by size:
2313 2219 1824 617 39
BsaAI yAC'GTr
Cuts at: 493 1204 2695 4803 493
Size: 711 1491 2108 2702
Fragments arranged by size:
2702 2108 1491 711
BsaBI GATnn'nnATC
Cuts at: 7006 7006
Size: 7012
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3030 3428 3858 4819
Size: 53 83 186 936 1497 398 430 961
Cuts at: 4819 5201 5836 275
Size: 382 635 1451
Fragments arranged by size:
1497 1451 961 936 635 430 398 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2457 5445 5843 6117 6264 6675 2457
Size: 2988 398 274 147 411 2794
Fragments arranged by size:
2988 2794 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3793 3823 1178
Size: 30 165 30 2390 30 4367
Fragments arranged by size:
4367 2390 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2732 2873 2936 3320 3532 4058 1186
Size: 1546 141 63 384 212 526 4140
Fragments arranged by size:
4140 1546 526 384 212 141 63
BseYI C'CCAG_C
Cuts at: 2373 6215 6711 2373
Size: 3842 496 2674
Fragments arranged by size:
3842 2674 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2489 2717 3296 1163
Size: 573 753 228 579 4879
Fragments arranged by size:
4879 753 579 573 228
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2912 4462 5223 6251 1540
Size: 275 186 911 1550 761 1028 2301
Fragments arranged by size:
2301 1550 1028 911 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3803 5073 5158 6229 3803
Size: 1270 85 1071 4586
Fragments arranged by size:
4586 1270 1071 85
BsmI GAATG_Cn'
Cuts at: 4049 4523 4616 4049
Size: 474 93 6445
Fragments arranged by size:
6445 474 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3170 3803 4331 5073 5158 6229 1391
Size: 399 1380 633 528 742 85 1071 2174
Fragments arranged by size:
2174 1380 1071 742 633 528 399 85
BspEI T'CCGG_A
Cuts at: 2457 6675 2457
Size: 4218 2794
BspHI T'CATG_A
Cuts at: 4901 6631 4901
Size: 1730 5282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2271 4160 4179 4367 844
Size: 1427 1889 19 188 3489
Fragments arranged by size:
3489 1889 1427 188 19
BsrBI CCG'CTC
Cuts at: 1394 1823 2977 3781 3787 4899 1394
Size: 429 1154 804 6 1112 3507
Fragments arranged by size:
3507 1154 1112 804 429 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 5507 5681 65
Size: 1183 4259 174 1396
Fragments arranged by size:
4259 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2907 3006 3026 5656 5843 1700
Size: 110 1097 99 20 2630 187 2869
Fragments arranged by size:
2869 2630 1097 187 110 99 20
BsrGI T'GTAC_A
Cuts at: 96 2899 96
Size: 2803 4209
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7012
BssSI C'ACGA_G
Cuts at: 5072 6084 5072
Size: 1012 6000
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2366 2431 2602 794
Size: 1572 65 171 5204
Fragments arranged by size:
5204 1572 171 65
BstBI TT'CG_AA
Cuts at: 5816 5816
Size: 7012
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7012
BstXI CCAn_nnnn'nTGG
Cuts at: 3423 4000 3423
Size: 577 6435
BstYI r'GATC_y
Cuts at: 1972 2473 2563 3143 3903 4342 4709 5094 5111
Size: 501 90 580 760 439 367 385 17
Cuts at: 5111 6563 6649 6661 7007 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 760 580 501 439 385 367
346 90 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3242 3242
Size: 7012
BtgI C'CryG_G
Cuts at: 513 1065 2004 2409 3912 4308 513
Size: 552 939 405 1503 396 3217
Fragments arranged by size:
3217 1503 939 552 405 396
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2699 487
Size: 683 56 1473 4800
Fragments arranged by size:
4800 1473 683 56
BtsI GCAGTG_nn'
Cuts at: 2364 2625 4528 5322 5342 2364
Size: 261 1903 794 20 4034
Fragments arranged by size:
4034 1903 794 261 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7012
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6977
DraI TTT'AAA
Cuts at: 4316 4316
Size: 7012
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6019 809
Size: 507 4703 1802
Fragments arranged by size:
4703 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2909 3516
Size: 54 1425 50 275 18 312 767 607
Cuts at: 3516 4067 4459 5348 6977 8
Size: 551 392 889 1629 43
Fragments arranged by size:
1629 1425 889 767 607 551 392 312
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2909 4459 1537
Size: 275 1097 1550 4090
Fragments arranged by size:
4090 1550 1097 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2456 2729 4942 1353
Size: 1103 273 2213 3423
Fragments arranged by size:
3423 2213 1103 273
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5599 5973 6119 387
Size: 1064 138 4010 374 146 1280
Fragments arranged by size:
4010 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2258 2432 2456 2492 2813 2876
Size: 393 437 189 174 24 36 321 63
Cuts at: 2876 2939 3795 4110 4139 5075 5672 6459 6727
Size: 63 856 315 29 936 597 787 268
Cuts at: 6727 1239
Size: 1524
Fragments arranged by size:
1524 936 856 787 597 437 393 321
315 268 189 174 63 63 36 29
24
EcoO109I rG'GnC_Cy
Cuts at: 2890 3523 3605 2890
Size: 633 82 6297
Fragments arranged by size:
6297 633 82
EcoRI G'AATT_C
Cuts at: 3224 4321 4699 3224
Size: 1097 378 5537
Fragments arranged by size:
5537 1097 378
FspI TGC'GCA
Cuts at: 5518 5518
Size: 7012
HaeII r_GCGC'y
Cuts at: 1678 4107 6159 1678
Size: 2429 2052 2531
Fragments arranged by size:
2531 2429 2052
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2107 2139 2269 2301 3101
Size: 32 135 32 535 32 130 32 800
Cuts at: 3101 3133 3445 3477 3552 3584 5653 5685 5727
Size: 32 312 32 75 32 2069 32 42
Cuts at: 5727 5759 1373
Size: 32 2626
Fragments arranged by size:
2626 2069 800 535 312 135 130 75
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2840 4284 4356 4602 669
Size: 988 281 902 1444 72 246 3079
Fragments arranged by size:
3079 1444 988 902 281 246 72
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7012
HpaI GTT'AAC
Cuts at: 4602 4602
Size: 7012
KpnI G_GTAC'C
Cuts at: 2047 4337 2047
Size: 2290 4722
MfeI C'AATT_G
Cuts at: 3277 3495 4611 3277
Size: 218 1116 5678
Fragments arranged by size:
5678 1116 218
MluI A'CGCG_T
Cuts at: 6888 6888
Size: 7012
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3225 3423 6126 6310 1305
Size: 1920 198 2703 184 2007
Fragments arranged by size:
2703 2007 1920 198 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5190
Fragments arranged by size:
5190 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 2993 3179 3872 4970 5329 5488
Size: 648 1002 825 186 693 1098 359 159
Cuts at: 5488 518
Size: 2042
Fragments arranged by size:
2042 1098 1002 825 693 648 359 186
159
MspA1I CmG'CkG
Cuts at: 1606 2611 3577 3891 3937 4428 5105 6253 6498
Size: 1005 966 314 46 491 677 1148 245
Cuts at: 6498 1606
Size: 2120
Fragments arranged by size:
2120 1148 1005 966 677 491 314 245
46
NcoI C'CATG_G
Cuts at: 513 1065 2004 4308 513
Size: 552 939 2304 3217
Fragments arranged by size:
3217 2304 939 552
NdeI CA'TA_TG
Cuts at: 387 2137 387
Size: 1750 5262
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7012
NotI GC'GGCC_GC
Cuts at: 4459 4459
Size: 7012
NsiI A_TGCA'T
Cuts at: 3000 3000
Size: 7012
NspI r_CATG'y
Cuts at: 1132 1229 2162 2560 2992 3451 3949 4370 5915
Size: 97 933 398 432 459 498 421 1545
Cuts at: 5915 1132
Size: 2229
Fragments arranged by size:
2229 1545 933 498 459 432 421 398
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7012
PciI A'CATG_T
Cuts at: 3945 5911 3945
Size: 1966 5046
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3438 3913 1108
Size: 2330 475 4207
Fragments arranged by size:
4207 2330 475
PfoI T'CCnGG_A
Cuts at: 2508 3146 2508
Size: 638 6374
PmeI GTTT'AAAC
Cuts at: 4316 4316
Size: 7012
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2607 2639 5081 5113 6594 6626 2607
Size: 32 2442 32 1481 32 2993
Fragments arranged by size:
2993 2442 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2890 3523 3605 2890
Size: 633 82 6297
Fragments arranged by size:
6297 633 82
PshAI GACnn'nnGTC
Cuts at: 1613 3029 1613
Size: 1416 5596
PsiI TTA'TAA
Cuts at: 4582 4582
Size: 7012
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3737 3769 3737
Size: 32 6980
PstI C_TGCA'G
Cuts at: 830 2362 3223 4157 4364 830
Size: 1532 861 934 207 3478
Fragments arranged by size:
3478 1532 934 861 207
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7012
PvuII CAG'CTG
Cuts at: 2611 3577 3937 2611
Size: 966 360 5686
Fragments arranged by size:
5686 966 360
SalI G'TCGA_C
Cuts at: 1936 4354 1936
Size: 2418 4594
SanDI GG'GwC_CC
Cuts at: 2890 2890
Size: 7012
SbfI CC_TGCA'GG
Cuts at: 4364 4364
Size: 7012
ScaI AGT'ACT
Cuts at: 5260 6972 5260
Size: 1712 5300
SexAI A'CCwGG_T
Cuts at: 3607 3607
Size: 7012
SfcI C'TryA_G
Cuts at: 826 1045 2024 2068 2351 2358 3219 4153 4360
Size: 219 979 44 283 7 861 934 207
Cuts at: 4360 5495 6176 6367 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 979 934 861 681 283 219
207 191 44 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7012
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7012
SmaI CCC'GGG
Cuts at: 1872 4339 1872
Size: 2467 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2288 2540 3353 3796 5116 6017
Size: 197 926 345 252 813 443 1320 901
Cuts at: 6017 6279 820
Size: 262 1553
Fragments arranged by size:
1553 1320 926 901 813 443 345 262
252 197
SnaBI TAC'GTA
Cuts at: 493 4803 493
Size: 4310 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7012
SphI G_CATG'C
Cuts at: 1132 2992 4370 1132
Size: 1860 1378 3774
Fragments arranged by size:
3774 1860 1378
SspI AAT'ATT
Cuts at: 5 52 4936 5
Size: 47 4884 2081
Fragments arranged by size:
4884 2081 47
StuI AGG'CCT
Cuts at: 1517 3351 1517
Size: 1834 5178
StyI C'CwwG_G
Cuts at: 513 1065 2004 2111 3346 4038 4308 4451 513
Size: 552 939 107 1235 692 270 143 3074
Fragments arranged by size:
3074 1235 939 692 552 270 143 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3347 3862 5039 5056 5209 5394 5839
Size: 1255 1767 515 1177 17 153 185 445
Cuts at: 5839 325
Size: 1498
Fragments arranged by size:
1767 1498 1255 1177 515 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2899 3726 5258
Size: 274 80 33 51 1443 922 827 1532
Cuts at: 5258 6970 96
Size: 1712 138
Fragments arranged by size:
1712 1532 1443 922 827 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3597 3629 3793 3825 6972 7004 3597
Size: 32 164 32 3147 32 3605
Fragments arranged by size:
3605 3147 164 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7012
XbaI T'CTAG_A
Cuts at: 2476 4348 2476
Size: 1872 5140
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2407 3501 2407
Size: 1094 5918
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7012
XmaI C'CCGG_G
Cuts at: 1870 4337 1870
Size: 2467 4545
XmnI GAAnn'nnTTC
Cuts at: 5141 5141
Size: 7012
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4820 276
Size: 53 83 186 936 3286 2468
Fragments arranged by size:
3286 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI RsrII SacI SacII SapI SfiI
SfoI SrfI SwaI