Restriction map for
pFN21AB5089
(Circular) (Six-base) MAPSORT of: pFN21AB5089.seq Check: 544 from: 1 to: 8407
pFN21AB5089 8407 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2482 3419 5004 2482
Size: 937 1585 5885
Fragments arranged by size:
5885 1585 937
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6217 278
Size: 53 83 186 936 4681 2468
Fragments arranged by size:
4681 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5750 1937
Size: 3813 4594
Acc65I G'GTAC_C
Cuts at: 4095 5728 4095
Size: 1633 6774
AclI AA'CG_TT
Cuts at: 1563 6534 6907 1563
Size: 4971 373 3063
Fragments arranged by size:
4971 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2504 2552 6470 7854 8122 1632
Size: 872 48 3918 1384 268 1917
Fragments arranged by size:
3918 1917 1384 872 268 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8210
AflIII A'CryG_T
Cuts at: 1203 3096 4254 7306 8283 1203
Size: 1893 1158 3052 977 1327
Fragments arranged by size:
3052 1893 1327 1158 977
AgeI A'CCGG_T
Cuts at: 7238 7238
Size: 8407
AhdI GACnn_n'nnGTC
Cuts at: 2112 2875 4690 7136 2112
Size: 763 1815 2446 3383
Fragments arranged by size:
3383 2446 1815 763
AleI CACnn'nnGTG
Cuts at: 1166 2057 3161 3962 1166
Size: 891 1104 801 5611
Fragments arranged by size:
5611 1104 891 801
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8407
AlwNI CAG_nnn'CTG
Cuts at: 2860 2971 3379 4216 4702 5128 5197 5476 6150
Size: 111 408 837 486 426 69 279 674
Cuts at: 6150 7722 2860
Size: 1572 3545
Fragments arranged by size:
3545 1572 837 674 486 426 408 279
111 69
ApaI G_GGCC'C
Cuts at: 2174 4624 2174
Size: 2450 5957
ApaLI G'TGCA_C
Cuts at: 6464 7620 6464
Size: 1156 7251
ApoI r'AATT_y
Cuts at: 1493 2591 4415 5716 5942 6094 8203 1493
Size: 1098 1824 1301 226 152 2109 1697
Fragments arranged by size:
2109 1824 1697 1301 1098 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6961 160
Size: 872 5929 1606
Fragments arranged by size:
5929 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8407
AvaI C'yCGr_G
Cuts at: 1870 1943 2773 5645 5732 1870
Size: 73 830 2872 87 4545
Fragments arranged by size:
4545 2872 830 87 73
AvrII C'CTAG_G
Cuts at: 5038 5038
Size: 8407
BamHI G'GATC_C
Cuts at: 2511 4431 5737 6104 2511
Size: 1920 1306 367 4814
Fragments arranged by size:
4814 1920 1306 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 2705 3163 3414 3956
Size: 325 217 627 225 693 458 251 542
Cuts at: 3956 4008 4095 4425 5016 5033 5658 5728 7183
Size: 52 87 330 591 17 625 70 1455
Cuts at: 7183 618
Size: 1842
Fragments arranged by size:
1842 1455 693 627 625 591 542 458
330 325 251 225 217 87 70 52
17
BanII G_rGCy'C
Cuts at: 1391 2174 2847 3497 3513 3902 4344 4624 4875
Size: 783 673 650 16 389 442 280 251
Cuts at: 4875 5502 5726 1391
Size: 627 224 4072
Fragments arranged by size:
4072 783 673 650 627 442 389 280
251 224 16
BbeI G_GCGC'C
Cuts at: 2016 2709 2016
Size: 693 7714
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7436
BbvCI CC'TCA_GC
Cuts at: 4799 5007 4799
Size: 208 8199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3479 3513 3740 3774 6598 6632 1903
Size: 34 1542 34 227 34 2824 34 3678
Fragments arranged by size:
3678 2824 1542 227 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3401 4454 6299 7509 3401
Size: 1053 1845 1210 4299
Fragments arranged by size:
4299 1845 1210 1053
BclI T'GATC_A
Cuts at: 1548 4581 1548
Size: 3033 5374
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2806 5519 5580
Size: 107 122 71 631 605 1134 2713 61
Cuts at: 5580 5596 7018 136
Size: 16 1422 1525
Fragments arranged by size:
2713 1525 1422 1134 631 605 122 107
71 61 16
BglII A'GATC_T
Cuts at: 8402 8402
Size: 8407
BlpI GC'TnA_GC
Cuts at: 3935 5824 3935
Size: 1889 6518
Bme1580I G_kGCm'C
Cuts at: 1790 2174 3740 3959 4428 4624 5038 5661 6468
Size: 384 1566 219 469 196 414 623 807
Cuts at: 6468 7624 1790
Size: 1156 2573
Fragments arranged by size:
2573 1566 1156 807 623 469 414 384
219 196
BmgBI CAC'GTC
Cuts at: 1318 3097 3178 4600 4677 1318
Size: 1779 81 1422 77 5048
Fragments arranged by size:
5048 1779 1422 81 77
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4684 6138 7096
Size: 416 53 83 399 24 3267 1454 958
Cuts at: 7096 442
Size: 1753
Fragments arranged by size:
3267 1753 1454 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8407
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2125 3532 4674 5211 2125
Size: 1407 1142 537 5321
Fragments arranged by size:
5321 1407 1142 537
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2095 2321 2717 2753 2882 3203 3485 3620
Size: 856 226 396 36 129 321 282 135
Cuts at: 3620 5153 5441 7067 1239
Size: 1533 288 1626 2579
Fragments arranged by size:
2579 1626 1533 856 396 321 288 282
226 135 129 36
Bpu10I CC'TnA_GC
Cuts at: 3485 3560 4659 4799 4995 5007 5207 3485
Size: 75 1099 140 196 12 200 6685
Fragments arranged by size:
6685 1099 200 196 140 75 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3605 4088 6532 7397 7695 3605
Size: 483 2444 865 298 4317
Fragments arranged by size:
4317 2444 865 483 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2057 2099 2862 3537 3863 4713 4914
Size: 617 558 42 763 675 326 850 201
Cuts at: 4914 7070 882
Size: 2156 2219
Fragments arranged by size:
2219 2156 850 763 675 617 558 326
201 42
BsaAI yAC'GTr
Cuts at: 493 1204 4243 6198 493
Size: 711 3039 1955 2702
Fragments arranged by size:
3039 2702 1955 711
BsaBI GATnn'nnATC
Cuts at: 8401 8401
Size: 8407
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2013 2025 2706 6214
Size: 53 83 186 936 480 12 681 3508
Cuts at: 6214 6596 7231 275
Size: 382 635 1451
Fragments arranged by size:
3508 1451 936 681 635 480 382 186
83 53 12
BsaWI w'CCGG_w
Cuts at: 2598 4428 4457 4902 6840 7238 7512 7659 8070
Size: 1830 29 445 1938 398 274 147 411
Cuts at: 8070 2598
Size: 2935
Fragments arranged by size:
2935 1938 1830 445 411 398 274 147
29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3278 3308 1178
Size: 30 165 30 1875 30 6277
Fragments arranged by size:
6277 1875 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2318 2417 2927 2983 3134 3505 3587
Size: 931 201 99 510 56 151 371 82
Cuts at: 3587 3692 3710 3782 3961 4418 5438 5510 5705
Size: 105 18 72 179 457 1020 72 195
Cuts at: 5705 1186
Size: 3888
Fragments arranged by size:
3888 1020 931 510 457 371 201 195
179 151 105 99 82 72 72 56
18
BseYI C'CCAG_C
Cuts at: 2016 2964 3029 3365 3422 3526 3536 3593 3987
Size: 948 65 336 57 104 10 57 394
Cuts at: 3987 4702 4733 5166 5459 5514 5578 5599 7610
Size: 715 31 433 293 55 64 21 2011
Cuts at: 7610 8106 2016
Size: 496 2317
Fragments arranged by size:
2317 2011 948 715 496 433 394 336
293 104 65 64 57 57 55 31
21 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2393 5141 1163
Size: 573 657 2748 4429
Fragments arranged by size:
4429 2748 657 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2675 3113 4127 5857 6618 7646
Size: 275 186 674 438 1014 1730 761 1028
Cuts at: 7646 1540
Size: 2301
Fragments arranged by size:
2301 1730 1028 1014 761 674 438 275
186
BsiHKAI G_wGCw'C
Cuts at: 2954 3497 4875 6468 6553 7624 2954
Size: 543 1378 1593 85 1071 3737
Fragments arranged by size:
3737 1593 1378 1071 543 85
BsmI GAATG_Cn'
Cuts at: 3763 5918 6011 3763
Size: 2155 93 6159
Fragments arranged by size:
6159 2155 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3300 4667 3300
Size: 1367 7040
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 2847 2954 3497 3513 3740 3902
Size: 399 384 673 107 543 16 227 162
Cuts at: 3902 3959 4344 4428 4624 4875 5038 5502 5661
Size: 57 385 84 196 251 163 464 159
Cuts at: 5661 5726 6468 6553 7624 1391
Size: 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 673 543 464 399 385
384 251 227 196 163 162 159 107
85 84 65 57 16
BspEI T'CCGG_A
Cuts at: 4457 4902 8070 4457
Size: 445 3168 4794
Fragments arranged by size:
4794 3168 445
BspHI T'CATG_A
Cuts at: 6296 8026 6296
Size: 1730 6677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2482 3419 3667 4107 4932 5004 5461 5762
Size: 1638 937 248 440 825 72 457 301
Cuts at: 5762 844
Size: 3489
Fragments arranged by size:
3489 1638 937 825 457 440 301 248
72
BsrBI CCG'CTC
Cuts at: 1394 1823 2647 3294 4455 6294 1394
Size: 429 824 647 1161 1839 3507
Fragments arranged by size:
3507 1839 1161 824 647 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3189 3571 4389 6902 7076 65
Size: 1183 1941 382 818 2513 174 1396
Fragments arranged by size:
2513 1941 1396 1183 818 382 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2229 4510 4758 4908 5019 7051 7238
Size: 110 419 2281 248 150 111 2032 187
Cuts at: 7238 1700
Size: 2869
Fragments arranged by size:
2869 2281 2032 419 248 187 150 111
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8407
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8407
BssSI C'ACGA_G
Cuts at: 6467 7479 6467
Size: 1012 7395
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5128 5476 794
Size: 4334 348 3725
Fragments arranged by size:
4334 3725 348
BstBI TT'CG_AA
Cuts at: 7211 7211
Size: 8407
BstEII G'GTnAC_C
Cuts at: 1184 2125 1184
Size: 941 7466
BstXI CCAn_nnnn'nTGG
Cuts at: 2059 3880 4741 4864 5509 2059
Size: 1821 861 123 645 4957
Fragments arranged by size:
4957 1821 861 645 123
BstYI r'GATC_y
Cuts at: 1972 2259 2511 2688 2944 4431 5292 5562 5737
Size: 287 252 177 256 1487 861 270 175
Cuts at: 5737 6104 6489 6506 7958 8044 8056 8402 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1487 1452 861 385 367 346 287
270 256 252 177 175 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3117 3117
Size: 8407
BtgI C'CryG_G
Cuts at: 513 1065 2004 2055 2880 3139 3879 3903 513
Size: 552 939 51 825 259 740 24 5017
Fragments arranged by size:
5017 939 825 740 552 259 51 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4064 4813 487
Size: 683 56 2838 749 4081
Fragments arranged by size:
4081 2838 749 683 56
BtsI GCAGTG_nn'
Cuts at: 3012 5923 6717 6737 3012
Size: 2911 794 20 4682
Fragments arranged by size:
4682 2911 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8372
DraI TTT'AAA
Cuts at: 5711 5711
Size: 8407
DraIII CAC_nnn'GTG
Cuts at: 2975 2975
Size: 8407
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2323 7414 809
Size: 507 1007 5091 1802
Fragments arranged by size:
5091 1802 1007 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2367 2672 3110
Size: 54 1425 50 275 18 537 305 438
Cuts at: 3110 3501 4124 4176 4632 4666 5027 5854 6743
Size: 391 623 52 456 34 361 827 889
Cuts at: 6743 8372 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 827 623 537 456 438
391 361 305 275 54 52 50 43
34 18
EagI C'GGCC_G
Cuts at: 1537 1812 2672 3110 4124 5854 1537
Size: 275 860 438 1014 1730 4090
Fragments arranged by size:
4090 1730 1014 860 438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3687 3998 4332 5487 6337 1353
Size: 2334 311 334 1155 850 3423
Fragments arranged by size:
3423 2334 1155 850 334 311
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5375 5555 6994 7368 7514 387
Size: 1064 138 3786 180 1439 374 146 1280
Fragments arranged by size:
3786 1439 1280 1064 374 180 146 138
EcoICRI GAG'CTC
Cuts at: 3495 4873 3495
Size: 1378 7029
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2321 2504 2552 2717 2753 2882
Size: 393 463 226 183 48 165 36 129
Cuts at: 2882 3203 3485 3620 5153 5441 6470 7067 7854
Size: 321 282 135 1533 288 1029 597 787
Cuts at: 7854 8122 1239
Size: 268 1524
Fragments arranged by size:
1533 1524 1029 787 597 463 393 321
288 282 268 226 183 165 135 129
48 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2246 4844 4994 5189 2246
Size: 2598 150 195 5464
Fragments arranged by size:
5464 2598 195 150
EcoO109I rG'GnC_Cy
Cuts at: 2171 2251 2285 3033 3376 3628 3641 4576 4620
Size: 80 34 748 343 252 13 935 44
Cuts at: 4620 5473 2171
Size: 853 5105
Fragments arranged by size:
5105 935 853 748 343 252 80 44
34 13
EcoRI G'AATT_C
Cuts at: 5716 6094 5716
Size: 378 8029
FspI TGC'GCA
Cuts at: 2465 4937 6913 2465
Size: 2472 1976 3959
Fragments arranged by size:
3959 2472 1976
HaeII r_GCGC'y
Cuts at: 1678 2016 2709 3447 5311 5673 7554 1678
Size: 338 693 738 1864 362 1881 2531
Fragments arranged by size:
2531 1881 1864 738 693 362 338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2098 2130 2861 2893 3758
Size: 32 135 32 526 32 731 32 865
Cuts at: 3758 3790 3842 3874 4676 4708 5365 5397 7048
Size: 32 52 32 802 32 657 32 1651
Cuts at: 7048 7080 7122 7154 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1651 865 802 731 657 526 135
52 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3787 5404 5751 5997 669
Size: 988 281 1849 1617 347 246 3079
Fragments arranged by size:
3079 1849 1617 988 347 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8407
HpaI GTT'AAC
Cuts at: 5997 5997
Size: 8407
KasI G'GCGC_C
Cuts at: 2012 2705 2012
Size: 693 7714
KpnI G_GTAC'C
Cuts at: 4099 5732 4099
Size: 1633 6774
MfeI C'AATT_G
Cuts at: 6006 6006
Size: 8407
MluI A'CGCG_T
Cuts at: 8283 8283
Size: 8407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2022 2096 2489 3999 4245 7521 7705 1305
Size: 717 74 393 1510 246 3276 184 2007
Fragments arranged by size:
3276 2007 1510 717 393 246 184 74
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4178 4668 10
Size: 54 1425 343 2346 490 3749
Fragments arranged by size:
3749 2346 1425 490 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2183 2369 2957 3067 3161 3962
Size: 648 891 126 186 588 110 94 801
Cuts at: 3962 4241 6365 6724 6883 518
Size: 279 2124 359 159 2042
Fragments arranged by size:
2124 2042 891 801 648 588 359 279
186 159 126 110 94
MspA1I CmG'CkG
Cuts at: 1606 3025 3081 3343 3526 3549 4092 4501 4630
Size: 1419 56 262 183 23 543 409 129
Cuts at: 4630 4702 4864 4951 5051 5170 5203 5440 5485
Size: 72 162 87 100 119 33 237 45
Cuts at: 5485 5518 5542 5655 5823 6500 7648 7893 1606
Size: 33 24 113 168 677 1148 245 2120
Fragments arranged by size:
2120 1419 1148 677 543 409 262 245
237 183 168 162 129 119 113 100
87 72 56 45 33 33 24 23
NaeI GCC'GGC
Cuts at: 4512 4760 4910 5021 4512
Size: 248 150 111 7898
Fragments arranged by size:
7898 248 150 111
NarI GG'CG_CC
Cuts at: 2013 2706 2013
Size: 693 7714
NcoI C'CATG_G
Cuts at: 513 1065 2004 2880 3879 3903 513
Size: 552 939 876 999 24 5017
Fragments arranged by size:
5017 999 939 876 552 24
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8407
NgoMIV G'CCGG_C
Cuts at: 4510 4758 4908 5019 4510
Size: 248 150 111 7898
Fragments arranged by size:
7898 248 150 111
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8407
NotI GC'GGCC_GC
Cuts at: 5854 5854
Size: 8407
NspI r_CATG'y
Cuts at: 1132 1229 3985 4258 5765 7310 1132
Size: 97 2756 273 1507 1545 2229
Fragments arranged by size:
2756 2229 1545 1507 273 97
PasI CC'CwG_GG
Cuts at: 1348 4808 1348
Size: 3460 4947
PciI A'CATG_T
Cuts at: 4254 7306 4254
Size: 3052 5355
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2394 1108
Size: 1286 7121
PfoI T'CCnGG_A
Cuts at: 2262 2447 5295 2262
Size: 185 2848 5374
Fragments arranged by size:
5374 2848 185
PmeI GTTT'AAAC
Cuts at: 5711 5711
Size: 8407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6476 6508 7989 8021 6476
Size: 32 1481 32 6862
Fragments arranged by size:
6862 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2251 2285 3033 3376 5473 2251
Size: 34 748 343 2097 5185
Fragments arranged by size:
5185 2097 748 343 34
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8407
PsiI TTA'TAA
Cuts at: 5977 5977
Size: 8407
PspOMI G'GGCC_C
Cuts at: 2170 4620 2170
Size: 2450 5957
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5087 5119 5087
Size: 32 8375
PstI C_TGCA'G
Cuts at: 830 2040 2493 5202 5289 5759 830
Size: 1210 453 2709 87 470 3478
Fragments arranged by size:
3478 2709 1210 470 453 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8407
PvuII CAG'CTG
Cuts at: 3025 3526 3549 4630 4702 5485 5518 3025
Size: 501 23 1081 72 783 33 5914
Fragments arranged by size:
5914 1081 783 501 72 33 23
SacI G_AGCT'C
Cuts at: 3497 4875 3497
Size: 1378 7029
SalI G'TCGA_C
Cuts at: 1936 5749 1936
Size: 3813 4594
SanDI GG'GwC_CC
Cuts at: 2285 2285
Size: 8407
SapI GCTCTTCn'nnn_
Cuts at: 3998 4332 3998
Size: 334 8073
SbfI CC_TGCA'GG
Cuts at: 5759 5759
Size: 8407
ScaI AGT'ACT
Cuts at: 3287 6655 8367 3287
Size: 3368 1712 3327
Fragments arranged by size:
3368 3327 1712
SexAI A'CCwGG_T
Cuts at: 2121 2121
Size: 8407
SfcI C'TryA_G
Cuts at: 826 1045 2036 2489 3726 5198 5285 5755 6890
Size: 219 991 453 1237 1472 87 470 1135
Cuts at: 6890 7571 7762 826
Size: 681 191 1471
Fragments arranged by size:
1472 1471 1237 1135 991 681 470 453
219 191 87
SfiI GGCCn_nnn'nGGCC
Cuts at: 5519 5519
Size: 8407
SfoI GGC'GCC
Cuts at: 2014 2707 2014
Size: 693 7714
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8407
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8407
SmaI CCC'GGG
Cuts at: 1872 5647 5734 1872
Size: 3775 87 4545
Fragments arranged by size:
4545 3775 87
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3584 4103 6511 7412 7674 820
Size: 197 926 1641 519 2408 901 262 1553
Fragments arranged by size:
2408 1641 1553 926 901 519 262 197
SnaBI TAC'GTA
Cuts at: 493 6198 493
Size: 5705 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8407
SphI G_CATG'C
Cuts at: 1132 5765 1132
Size: 4633 3774
SspI AAT'ATT
Cuts at: 5 52 6331 5
Size: 47 6279 2081
Fragments arranged by size:
6279 2081 47
StuI AGG'CCT
Cuts at: 1517 3558 1517
Size: 2041 6366
StyI C'CwwG_G
Cuts at: 513 1065 2004 2247 2388 2631 2709 2880 3873
Size: 552 939 243 141 243 78 171 993
Cuts at: 3873 3879 3903 3951 4356 5038 5846 513
Size: 6 24 48 405 682 808 3074
Fragments arranged by size:
3074 993 939 808 682 552 405 243
243 171 141 78 48 24 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3608 4703 6434 6451 6604 6789 7234
Size: 1255 2028 1095 1731 17 153 185 445
Cuts at: 7234 325
Size: 1498
Fragments arranged by size:
2028 1731 1498 1255 1095 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2160 3285 4077
Size: 274 80 33 51 1443 183 1125 792
Cuts at: 4077 6653 8365 96
Size: 2576 1712 138
Fragments arranged by size:
2576 1712 1443 1125 792 274 183 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8367 8399 8367
Size: 32 8375
Tth111I GACn'n_nGTC
Cuts at: 1316 3037 1316
Size: 1721 6686
XbaI T'CTAG_A
Cuts at: 5743 5743
Size: 8407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2119 2182 3901 3910 5416 2119
Size: 63 1719 9 1506 5110
Fragments arranged by size:
5110 1719 1506 63 9
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8407
XmaI C'CCGG_G
Cuts at: 1870 5645 5732 1870
Size: 3775 87 4545
Fragments arranged by size:
4545 3775 87
XmnI GAAnn'nnTTC
Cuts at: 2456 2537 4316 6536 2456
Size: 81 1779 2220 4327
Fragments arranged by size:
4327 2220 1779 81
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6215 276
Size: 53 83 186 936 4681 2468
Fragments arranged by size:
4681 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
RsrII SacII SrfI SwaI