Restriction map for
pFN21AB5083
(Circular) (Six-base) MAPSORT of: pFN21AB5083.seq Check: 5909 from: 1 to: 6760
pFN21AB5083 6760 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4570 278
Size: 53 83 186 936 3034 2468
Fragments arranged by size:
3034 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2226 2325 2816 2894 4103 1937
Size: 289 99 491 78 1209 4594
Fragments arranged by size:
4594 1209 491 289 99 78
Acc65I G'GTAC_C
Cuts at: 2729 3555 4081 2729
Size: 826 526 5408
Fragments arranged by size:
5408 826 526
AclI AA'CG_TT
Cuts at: 1563 4059 4887 5260 1563
Size: 2496 828 373 3063
Fragments arranged by size:
3063 2496 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2772 3797 4823 6207 6475 1632
Size: 1140 1025 1026 1384 268 1917
Fragments arranged by size:
1917 1384 1140 1026 1025 268
AfeI AGC'GCT
Cuts at: 2246 2246
Size: 6760
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6563
AflIII A'CryG_T
Cuts at: 1203 5659 6636 1203
Size: 4456 977 1327
Fragments arranged by size:
4456 1327 977
AgeI A'CCGG_T
Cuts at: 5591 5591
Size: 6760
AhdI GACnn_n'nnGTC
Cuts at: 3031 5489 3031
Size: 2458 4302
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6760
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2952 1184
Size: 1768 4992
AlwNI CAG_nnn'CTG
Cuts at: 2068 3110 4503 6075 2068
Size: 1042 1393 1572 2753
Fragments arranged by size:
2753 1572 1393 1042
ApaI G_GGCC'C
Cuts at: 2800 2800
Size: 6760
ApaLI G'TGCA_C
Cuts at: 4817 5973 4817
Size: 1156 5604
ApoI r'AATT_y
Cuts at: 1493 2409 3088 3874 4069 4295 4447 6556 1493
Size: 916 679 786 195 226 152 2109 1697
Fragments arranged by size:
2109 1697 916 786 679 226 195 152
AseI AT'TA_AT
Cuts at: 160 1032 2643 5314 160
Size: 872 1611 2671 1606
Fragments arranged by size:
2671 1611 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6760
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 2197 4085 1870
Size: 73 177 77 1888 4545
Fragments arranged by size:
4545 1888 177 77 73
BamHI G'GATC_C
Cuts at: 4090 4457 4090
Size: 367 6393
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2729 3555 3860 4081 5536
Size: 325 217 627 942 826 305 221 1455
Cuts at: 5536 618
Size: 1842
Fragments arranged by size:
1842 1455 942 826 627 325 305 221
217
BanII G_rGCy'C
Cuts at: 1391 2800 3264 4079 1391
Size: 1409 464 815 4072
Fragments arranged by size:
4072 1409 815 464
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3027 3189 3402 3799 928
Size: 971 1128 162 213 397 3889
Fragments arranged by size:
3889 1128 971 397 213 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4951 4985 1903
Size: 34 3014 34 3678
Fragments arranged by size:
3678 3014 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4652 5862 4652
Size: 1210 5550
BclI T'GATC_A
Cuts at: 1548 3886 1548
Size: 2338 4422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5371 136
Size: 107 122 71 631 605 3699 1525
Fragments arranged by size:
3699 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2208 2617 3766 6755 2208
Size: 409 1149 2989 2213
Fragments arranged by size:
2989 2213 1149 409
BlpI GC'TnA_GC
Cuts at: 3494 4177 3494
Size: 683 6077
Bme1580I G_kGCm'C
Cuts at: 1790 2160 2800 4821 5977 1790
Size: 370 640 2021 1156 2573
Fragments arranged by size:
2573 2021 1156 640 370
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6760
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2247 2967 4491
Size: 416 53 83 399 24 830 720 1524
Cuts at: 4491 5449 442
Size: 958 1753
Fragments arranged by size:
1753 1524 958 830 720 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3248 5420 1239
Size: 2009 2172 2579
Fragments arranged by size:
2579 2172 2009
Bpu10I CC'TnA_GC
Cuts at: 3197 3690 3197
Size: 493 6267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4885 5750 6048 4885
Size: 865 298 5597
Fragments arranged by size:
5597 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5423 882
Size: 617 3924 2219
Fragments arranged by size:
3924 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4551 493
Size: 711 3347 2702
Fragments arranged by size:
3347 2702 711
BsaBI GATnn'nnATC
Cuts at: 6754 6754
Size: 6760
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4567 4949 5584 275
Size: 53 83 186 936 3034 382 635 1451
Fragments arranged by size:
3034 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3022 5193 5591 5865 6012 6423 3022
Size: 2171 398 274 147 411 3359
Fragments arranged by size:
3359 2171 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3670 3700 1178
Size: 30 165 30 2267 30 4238
Fragments arranged by size:
4238 2267 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3272 3758 1186
Size: 2086 486 4188
Fragments arranged by size:
4188 2086 486
BseYI C'CCAG_C
Cuts at: 2068 2266 3079 3236 3673 5963 6459 2068
Size: 198 813 157 437 2290 496 2369
Fragments arranged by size:
2369 2290 813 496 437 198 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2453 1163
Size: 573 717 5470
Fragments arranged by size:
5470 717 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2108 4210 4971 5999 1540
Size: 275 186 107 2102 761 1028 2301
Fragments arranged by size:
2301 2102 1028 761 275 186 107
BsiHKAI G_wGCw'C
Cuts at: 3264 3500 3604 4821 4906 5977 3264
Size: 236 104 1217 85 1071 4047
Fragments arranged by size:
4047 1217 1071 236 104 85
BsmI GAATG_Cn'
Cuts at: 3392 3477 4271 4364 3392
Size: 85 794 93 5788
Fragments arranged by size:
5788 794 93 85
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2800 3264 3500 3604 4079 4821
Size: 399 370 640 464 236 104 475 742
Cuts at: 4821 4906 5977 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 640 475 464 399 370
236 104 85
BspEI T'CCGG_A
Cuts at: 3022 6423 3022
Size: 3401 3359
BspHI T'CATG_A
Cuts at: 2593 4649 6379 2593
Size: 2056 1730 2974
Fragments arranged by size:
2974 2056 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4115 844
Size: 3271 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4647 1394
Size: 429 2824 3507
Fragments arranged by size:
3507 2824 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2909 5255 5429 65
Size: 1183 1661 2346 174 1396
Fragments arranged by size:
2346 1661 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5404 5591 1700
Size: 110 3594 187 2869
Fragments arranged by size:
3594 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6760
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6760
BssSI C'ACGA_G
Cuts at: 4820 5832 4820
Size: 1012 5748
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2079 2431 3110 3387 3683 794
Size: 1285 352 679 277 296 3871
Fragments arranged by size:
3871 1285 679 352 296 277
BstBI TT'CG_AA
Cuts at: 3790 5564 3790
Size: 1774 4986
BstEII G'GTnAC_C
Cuts at: 1184 3514 1184
Size: 2330 4430
BstYI r'GATC_y
Cuts at: 1972 2208 2617 3766 4090 4457 4842 4859 6311
Size: 236 409 1149 324 367 385 17 1452
Cuts at: 6311 6397 6409 6755 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1149 409 385 367 346 324
236 86 17 12
BstZ17I GTA'TAC
Cuts at: 2817 2895 2817
Size: 78 6682
Bsu36I CC'TnA_GG
Cuts at: 2561 2561
Size: 6760
BtgI C'CryG_G
Cuts at: 513 1065 2004 2652 3405 513
Size: 552 939 648 753 3868
Fragments arranged by size:
3868 939 753 648 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6021
Fragments arranged by size:
6021 683 56
BtsI GCAGTG_nn'
Cuts at: 3388 4276 5070 5090 3388
Size: 888 794 20 5058
Fragments arranged by size:
5058 888 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6760
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3265 3300 529
Size: 35 2701 35 3989
Fragments arranged by size:
3989 2701 35 35
DraI TTT'AAA
Cuts at: 4064 4064
Size: 6760
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3575 5767 809
Size: 507 2259 2192 1802
Fragments arranged by size:
2259 2192 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2105 3655 4207
Size: 54 1425 50 275 18 275 1550 552
Cuts at: 4207 5096 6725 8
Size: 889 1629 43
Fragments arranged by size:
1629 1550 1425 889 552 275 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2105 4207 1537
Size: 275 293 2102 4090
Fragments arranged by size:
4090 2102 293 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4690 1353
Size: 3337 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5347 5721 5867 387
Size: 1064 138 3758 374 146 1280
Fragments arranged by size:
3758 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3262 3262
Size: 6760
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2772 3248 3797 4823 5420 6207 6475
Size: 393 1140 476 549 1026 597 787 268
Cuts at: 6475 1239
Size: 1524
Fragments arranged by size:
1524 1140 1026 787 597 549 476 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2797 2835 3835 2797
Size: 38 1000 5722
Fragments arranged by size:
5722 1000 38
EcoRI G'AATT_C
Cuts at: 2409 4069 4447 2409
Size: 1660 378 4722
Fragments arranged by size:
4722 1660 378
EcoRV GAT'ATC
Cuts at: 3957 3957
Size: 6760
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2296 3109 3944 2296
Size: 813 835 5112
Fragments arranged by size:
5112 835 813
FspI TGC'GCA
Cuts at: 5266 5266
Size: 6760
HaeII r_GCGC'y
Cuts at: 1678 2069 2248 5907 1678
Size: 391 179 3659 2531
Fragments arranged by size:
3659 2531 391 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2544 2576 3017 3049 3786
Size: 32 135 32 972 32 441 32 737
Cuts at: 3786 3818 3941 3973 5401 5433 5475 5507 1373
Size: 32 123 32 1428 32 42 32 2626
Fragments arranged by size:
2626 1428 972 737 441 135 123 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2227 2535 4104 4350 669
Size: 988 281 289 308 1569 246 3079
Fragments arranged by size:
3079 1569 988 308 289 281 246
HindIII A'AGCT_T
Cuts at: 748 3194 748
Size: 2446 4314
HpaI GTT'AAC
Cuts at: 4350 4350
Size: 6760
KpnI G_GTAC'C
Cuts at: 2733 3559 4085 2733
Size: 826 526 5408
Fragments arranged by size:
5408 826 526
MfeI C'AATT_G
Cuts at: 4359 4359
Size: 6760
MluI A'CGCG_T
Cuts at: 6636 6636
Size: 6760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5874 6058 1305
Size: 4569 184 2007
Fragments arranged by size:
4569 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3657 10
Size: 54 1425 343 1825 3113
Fragments arranged by size:
3113 1825 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2051 2424 2778 3508 4718 5077 5236
Size: 648 885 373 354 730 1210 359 159
Cuts at: 5236 518
Size: 2042
Fragments arranged by size:
2042 1210 885 730 648 373 359 354
159
MspA1I CmG'CkG
Cuts at: 1606 3107 3369 3493 4176 4853 6001 6246 1606
Size: 1501 262 124 683 677 1148 245 2120
Fragments arranged by size:
2120 1501 1148 683 677 262 245 124
NcoI C'CATG_G
Cuts at: 513 1065 2004 2652 3405 513
Size: 552 939 648 753 3868
Fragments arranged by size:
3868 939 753 648 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6760
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6760
NotI GC'GGCC_GC
Cuts at: 2105 4207 2105
Size: 2102 4658
NspI r_CATG'y
Cuts at: 1132 1229 2429 3463 4042 4118 5663 1132
Size: 97 1200 1034 579 76 1545 2229
Fragments arranged by size:
2229 1545 1200 1034 579 97 76
PasI CC'CwG_GG
Cuts at: 1348 2800 1348
Size: 1452 5308
PciI A'CATG_T
Cuts at: 5659 5659
Size: 6760
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6760
PmeI GTTT'AAAC
Cuts at: 4064 4064
Size: 6760
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4829 4861 6342 6374 4829
Size: 32 1481 32 5215
Fragments arranged by size:
5215 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2676 1613
Size: 1063 5697
PsiI TTA'TAA
Cuts at: 4330 4330
Size: 6760
PspOMI G'GGCC_C
Cuts at: 2796 2796
Size: 6760
PstI C_TGCA'G
Cuts at: 830 2492 4112 830
Size: 1662 1620 3478
Fragments arranged by size:
3478 1662 1620
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6760
PvuII CAG'CTG
Cuts at: 3107 3369 3493 3107
Size: 262 124 6374
Fragments arranged by size:
6374 262 124
SacI G_AGCT'C
Cuts at: 3264 3264
Size: 6760
SalI G'TCGA_C
Cuts at: 1936 2225 4102 1936
Size: 289 1877 4594
Fragments arranged by size:
4594 1877 289
SbfI CC_TGCA'GG
Cuts at: 4112 4112
Size: 6760
ScaI AGT'ACT
Cuts at: 5008 6720 5008
Size: 1712 5048
SfcI C'TryA_G
Cuts at: 826 1045 2488 3370 3907 4108 5243 5924 6115
Size: 219 1443 882 537 201 1135 681 191
Cuts at: 6115 826
Size: 1471
Fragments arranged by size:
1471 1443 1135 882 681 537 219 201
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6760
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6760
SmaI CCC'GGG
Cuts at: 1872 4087 1872
Size: 2215 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4864 5765 6027 820
Size: 197 926 2921 901 262 1553
Fragments arranged by size:
2921 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4551 493
Size: 4058 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6760
SphI G_CATG'C
Cuts at: 1132 4118 1132
Size: 2986 3774
SspI AAT'ATT
Cuts at: 5 52 4684 5
Size: 47 4632 2081
Fragments arranged by size:
4632 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6760
StyI C'CwwG_G
Cuts at: 513 1065 2004 2652 2700 3405 4199 513
Size: 552 939 648 48 705 794 3074
Fragments arranged by size:
3074 939 794 705 648 552 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2666 4787 4804 4957 5142 5587 325
Size: 1255 1086 2121 17 153 185 445 1498
Fragments arranged by size:
2121 1498 1255 1086 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5006 6718 96
Size: 274 80 33 51 1443 3029 1712 138
Fragments arranged by size:
3029 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6720 6752 6720
Size: 32 6728
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6760
XbaI T'CTAG_A
Cuts at: 4096 4096
Size: 6760
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6760
XmaI C'CCGG_G
Cuts at: 1870 4085 1870
Size: 2215 4545
XmnI GAAnn'nnTTC
Cuts at: 3927 4889 3927
Size: 962 5798
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4568 276
Size: 53 83 186 936 3034 2468
Fragments arranged by size:
3034 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI DraIII EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI