Restriction map for
pFN21AB5072
(Circular) (Six-base) MAPSORT of: pFN21AB5072.seq Check: 1297 from: 1 to: 6553
pFN21AB5072 6553 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4363 278
Size: 53 83 186 936 2827 2468
Fragments arranged by size:
2827 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2955 3896 1937
Size: 1018 941 4594
Fragments arranged by size:
4594 1018 941
Acc65I G'GTAC_C
Cuts at: 3874 3874
Size: 6553
AclI AA'CG_TT
Cuts at: 1563 4680 5053 1563
Size: 3117 373 3063
Fragments arranged by size:
3117 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2340 2810 3633 4616 6000 6268 1632
Size: 708 470 823 983 1384 268 1917
Fragments arranged by size:
1917 1384 983 823 708 470 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6356
AflIII A'CryG_T
Cuts at: 1203 5452 6429 1203
Size: 4249 977 1327
Fragments arranged by size:
4249 1327 977
AgeI A'CCGG_T
Cuts at: 5384 5384
Size: 6553
AhdI GACnn_n'nnGTC
Cuts at: 5282 5282
Size: 6553
AleI CACnn'nnGTG
Cuts at: 1166 3407 1166
Size: 2241 4312
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2366 1184
Size: 1182 5371
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2147 2179 2147
Size: 32 6521
AlwNI CAG_nnn'CTG
Cuts at: 2226 4296 5868 2226
Size: 2070 1572 2911
Fragments arranged by size:
2911 2070 1572
ApaLI G'TGCA_C
Cuts at: 4610 5766 4610
Size: 1156 5397
ApoI r'AATT_y
Cuts at: 1493 3104 3521 3862 4088 4240 6349 1493
Size: 1611 417 341 226 152 2109 1697
Fragments arranged by size:
2109 1697 1611 417 341 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5107 160
Size: 872 4075 1606
Fragments arranged by size:
4075 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
AvaI C'yCGr_G
Cuts at: 1870 1943 2369 2449 2649 2927 3147 3878 1870
Size: 73 426 80 200 278 220 731 4545
Fragments arranged by size:
4545 731 426 278 220 200 80 73
BamHI G'GATC_C
Cuts at: 3883 4250 3883
Size: 367 6186
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2557 2783 3874 5329 618
Size: 325 217 627 770 226 1091 1455 1842
Fragments arranged by size:
1842 1455 1091 770 627 325 226 217
BanII G_rGCy'C
Cuts at: 1391 3148 3872 1391
Size: 1757 724 4072
Fragments arranged by size:
4072 1757 724
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2991 3180 928
Size: 971 1092 189 4301
Fragments arranged by size:
4301 1092 971 189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4744 4778 1903
Size: 34 2807 34 3678
Fragments arranged by size:
3678 2807 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2050 4445 5655 2050
Size: 2395 1210 2948
Fragments arranged by size:
2948 2395 1210
BclI T'GATC_A
Cuts at: 1548 3478 1548
Size: 1930 4623
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2036 5164 136
Size: 107 122 71 631 605 364 3128 1525
Fragments arranged by size:
3128 1525 631 605 364 122 107 71
BglII A'GATC_T
Cuts at: 6548 6548
Size: 6553
BlpI GC'TnA_GC
Cuts at: 2018 3970 2018
Size: 1952 4601
Bme1580I G_kGCm'C
Cuts at: 1790 2355 2562 4614 5770 1790
Size: 565 207 2052 1156 2573
Fragments arranged by size:
2573 2052 1156 565 207
BmgBI CAC'GTC
Cuts at: 1318 3784 1318
Size: 2466 4087
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4284 5242 442
Size: 416 53 83 399 24 2867 958 1753
Fragments arranged by size:
2867 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2376 2659 5213 1239
Size: 1137 283 2554 2579
Fragments arranged by size:
2579 2554 1137 283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4678 5543 5841 4678
Size: 865 298 5390
Fragments arranged by size:
5390 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3734 5216 882
Size: 617 2235 1482 2219
Fragments arranged by size:
2235 2219 1482 617
BsaAI yAC'GTr
Cuts at: 493 1204 4344 493
Size: 711 3140 2702
Fragments arranged by size:
3140 2702 711
BsaBI GATnn'nnATC
Cuts at: 6547 6547
Size: 6553
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2988 4360 4742 5377
Size: 53 83 186 936 1455 1372 382 635
Cuts at: 5377 275
Size: 1451
Fragments arranged by size:
1455 1451 1372 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4986 5384 5658 5805 6216 4986
Size: 398 274 147 411 5323
Fragments arranged by size:
5323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3135 3165 1178
Size: 30 165 30 1732 30 4566
Fragments arranged by size:
4566 1732 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2379 1186
Size: 1193 5360
BseYI C'CCAG_C
Cuts at: 2410 2430 2516 5756 6252 2410
Size: 20 86 3240 496 2711
Fragments arranged by size:
3240 2711 496 86 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5980
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4003 4764 5792 1540
Size: 275 186 2002 761 1028 2301
Fragments arranged by size:
2301 2002 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2608 4614 4699 5770 2608
Size: 2006 85 1071 3391
Fragments arranged by size:
3391 2006 1071 85
BsmI GAATG_Cn'
Cuts at: 3700 4064 4157 3700
Size: 364 93 6096
Fragments arranged by size:
6096 364 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2355 2562 2608 3148 3872 4614 4699
Size: 399 565 207 46 540 724 742 85
Cuts at: 4699 5770 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 724 565 540 399 207
85 46
BspEI T'CCGG_A
Cuts at: 6216 6216
Size: 6553
BspHI T'CATG_A
Cuts at: 2139 3825 4442 6172 2139
Size: 1686 617 1730 2520
Fragments arranged by size:
2520 1730 1686 617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2501 3908 844
Size: 1657 1407 3489
Fragments arranged by size:
3489 1657 1407
BsrBI CCG'CTC
Cuts at: 1394 1823 3300 4440 1394
Size: 429 1477 1140 3507
Fragments arranged by size:
3507 1477 1140 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2031 2562 3201 5048 5222 65
Size: 1183 783 531 639 1847 174 1396
Fragments arranged by size:
1847 1396 1183 783 639 531 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5197 5384 1700
Size: 110 3387 187 2869
Fragments arranged by size:
3387 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6553
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6553
BssSI C'ACGA_G
Cuts at: 2198 2607 4613 5625 2198
Size: 409 2006 1012 3126
Fragments arranged by size:
3126 2006 1012 409
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6553
BstBI TT'CG_AA
Cuts at: 5357 5357
Size: 6553
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6553
BstXI CCAn_nnnn'nTGG
Cuts at: 3778 3778
Size: 6553
BstYI r'GATC_y
Cuts at: 1972 2628 2967 3883 4250 4635 4652 6104 6190
Size: 656 339 916 367 385 17 1452 86
Cuts at: 6190 6202 6548 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 916 656 385 367 346 339
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3792 513
Size: 552 939 1788 3274
Fragments arranged by size:
3274 1788 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5814
Fragments arranged by size:
5814 683 56
BtsI GCAGTG_nn'
Cuts at: 4069 4863 4883 4069
Size: 794 20 5739
Fragments arranged by size:
5739 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6553
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6518
DraI TTT'AAA
Cuts at: 3857 3857
Size: 6553
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5560 809
Size: 507 4244 1802
Fragments arranged by size:
4244 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2011 2037 3399
Size: 54 1425 50 275 18 181 26 1362
Cuts at: 3399 3811 4000 4889 6518 8
Size: 412 189 889 1629 43
Fragments arranged by size:
1629 1425 1362 889 412 275 189 181
54 50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 4000 1537
Size: 275 2188 4090
Fragments arranged by size:
4090 2188 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4483 1353
Size: 3130 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2109 3318 5140 5514 5660 387
Size: 1064 138 520 1209 1822 374 146 1280
Fragments arranged by size:
1822 1280 1209 1064 520 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2340 2376 2659 2810 3633 4616 5213
Size: 393 708 36 283 151 823 983 597
Cuts at: 5213 6000 6268 1239
Size: 787 268 1524
Fragments arranged by size:
1524 983 823 787 708 597 393 283
268 151 36
EcoO109I rG'GnC_Cy
Cuts at: 2427 2513 2427
Size: 86 6467
EcoRI G'AATT_C
Cuts at: 3862 4240 3862
Size: 378 6175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2681 2966 3638 2681
Size: 285 672 5596
Fragments arranged by size:
5596 672 285
FspI TGC'GCA
Cuts at: 5059 5059
Size: 6553
HaeII r_GCGC'y
Cuts at: 1678 2399 5700 1678
Size: 721 3301 2531
Fragments arranged by size:
3301 2531 721
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2271 2303 2329 2361 2921
Size: 32 135 32 699 32 26 32 560
Cuts at: 2921 2953 3135 3159 3167 3191 3335 3367 3714
Size: 32 182 24 8 24 144 32 347
Cuts at: 3714 3746 5194 5226 5268 5300 1373
Size: 32 1448 32 42 32 2626
Fragments arranged by size:
2626 1448 699 560 347 182 144 135
42 32 32 32 32 32 32 32
32 32 26 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2686 3897 4143 669
Size: 988 281 748 1211 246 3079
Fragments arranged by size:
3079 1211 988 748 281 246
HindIII A'AGCT_T
Cuts at: 748 2663 3233 748
Size: 1915 570 4068
Fragments arranged by size:
4068 1915 570
HpaI GTT'AAC
Cuts at: 4143 4143
Size: 6553
KpnI G_GTAC'C
Cuts at: 3878 3878
Size: 6553
MfeI C'AATT_G
Cuts at: 2213 4152 2213
Size: 1939 4614
MluI A'CGCG_T
Cuts at: 6429 6429
Size: 6553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2308 3289 5667 5851 1305
Size: 1003 981 2378 184 2007
Fragments arranged by size:
2378 2007 1003 981 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3401 10
Size: 54 1425 343 1569 3162
Fragments arranged by size:
3162 1569 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3407 3776 4511 4870 5029 518
Size: 648 2241 369 735 359 159 2042
Fragments arranged by size:
2241 2042 735 648 369 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2318 2816 3501 3969 4646 5794 6039
Size: 411 301 498 685 468 677 1148 245
Cuts at: 6039 1606
Size: 2120
Fragments arranged by size:
2120 1148 685 677 498 468 411 301
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5062
Fragments arranged by size:
5062 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6553
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6553
NotI GC'GGCC_GC
Cuts at: 4000 4000
Size: 6553
NspI r_CATG'y
Cuts at: 1132 1229 3436 3911 5456 1132
Size: 97 2207 475 1545 2229
Fragments arranged by size:
2229 2207 1545 475 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6553
PciI A'CATG_T
Cuts at: 5452 5452
Size: 6553
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6553
PfoI T'CCnGG_A
Cuts at: 3059 3600 3059
Size: 541 6012
PmeI GTTT'AAAC
Cuts at: 3857 3857
Size: 6553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4622 4654 6135 6167 4622
Size: 32 1481 32 5008
Fragments arranged by size:
5008 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2513 2513
Size: 6553
PshAI GACnn'nnGTC
Cuts at: 1613 2858 1613
Size: 1245 5308
PsiI TTA'TAA
Cuts at: 4123 4123
Size: 6553
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3419 3451 3419
Size: 32 6521
PstI C_TGCA'G
Cuts at: 830 2512 3905 830
Size: 1682 1393 3478
Fragments arranged by size:
3478 1682 1393
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6553
PvuII CAG'CTG
Cuts at: 2017 2318 2816 2017
Size: 301 498 5754
Fragments arranged by size:
5754 498 301
SalI G'TCGA_C
Cuts at: 1936 3895 1936
Size: 1959 4594
SbfI CC_TGCA'GG
Cuts at: 3905 3905
Size: 6553
ScaI AGT'ACT
Cuts at: 4801 6513 4801
Size: 1712 4841
SfcI C'TryA_G
Cuts at: 826 1045 2508 2709 3799 3901 5036 5717 5908
Size: 219 1463 201 1090 102 1135 681 191
Cuts at: 5908 826
Size: 1471
Fragments arranged by size:
1471 1463 1135 1090 681 219 201 191
102
SfiI GGCCn_nnn'nGGCC
Cuts at: 2036 2036
Size: 6553
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6553
SmaI CCC'GGG
Cuts at: 1872 3880 1872
Size: 2008 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2927 4657 5558 5820 820
Size: 197 926 984 1730 901 262 1553
Fragments arranged by size:
1730 1553 984 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4344 493
Size: 3851 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6553
SphI G_CATG'C
Cuts at: 1132 3911 1132
Size: 2779 3774
SspI AAT'ATT
Cuts at: 5 52 3562 4477 5
Size: 47 3510 915 2081
Fragments arranged by size:
3510 2081 915 47
StuI AGG'CCT
Cuts at: 1517 3227 1517
Size: 1710 4843
StyI C'CwwG_G
Cuts at: 513 1065 2004 3367 3622 3992 513
Size: 552 939 1363 255 370 3074
Fragments arranged by size:
3074 1363 939 552 370 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3577 4580 4597 4750 4935 5380 325
Size: 1255 1997 1003 17 153 185 445 1498
Fragments arranged by size:
1997 1498 1255 1003 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4799 6511 96
Size: 274 80 33 51 1443 2822 1712 138
Fragments arranged by size:
2822 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2587 2619 3508 3540 6513 6545 2587
Size: 32 889 32 2973 32 2595
Fragments arranged by size:
2973 2595 889 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6553
XbaI T'CTAG_A
Cuts at: 3116 3889 3116
Size: 773 5780
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2438 2438
Size: 6553
XhoI C'TCGA_G
Cuts at: 1943 2927 1943
Size: 984 5569
XmaI C'CCGG_G
Cuts at: 1870 3878 1870
Size: 2008 4545
XmnI GAAnn'nnTTC
Cuts at: 2458 2666 4682 2458
Size: 208 2016 4329
Fragments arranged by size:
4329 2016 208
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4361 276
Size: 53 83 186 936 2827 2468
Fragments arranged by size:
2827 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfoI SrfI SwaI