Restriction map for
pFN21AB5066
(Circular) (Six-base) MAPSORT of: pFN21AB5066.seq Check: 1718 from: 1 to: 6430
pFN21AB5066 6430 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2193 3122 2193
Size: 929 5501
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4240 278
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2606 3773 1937
Size: 669 1167 4594
Fragments arranged by size:
4594 1167 669
Acc65I G'GTAC_C
Cuts at: 3751 3751
Size: 6430
AclI AA'CG_TT
Cuts at: 1563 4557 4930 1563
Size: 2994 373 3063
Fragments arranged by size:
3063 2994 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2051 2238 2367 2869 3205 4493 5877 6145
Size: 419 187 129 502 336 1288 1384 268
Cuts at: 6145 1632
Size: 1917
Fragments arranged by size:
1917 1384 1288 502 419 336 268 187
129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 5329 6306 1203
Size: 4126 977 1327
Fragments arranged by size:
4126 1327 977
AgeI A'CCGG_T
Cuts at: 5261 5261
Size: 6430
AhdI GACnn_n'nnGTC
Cuts at: 2178 5159 2178
Size: 2981 3449
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6430
AlwNI CAG_nnn'CTG
Cuts at: 2446 2703 3295 4173 5745 2446
Size: 257 592 878 1572 3131
Fragments arranged by size:
3131 1572 878 592 257
ApaLI G'TGCA_C
Cuts at: 2825 3662 4487 5643 2825
Size: 837 825 1156 3612
Fragments arranged by size:
3612 1156 837 825
ApoI r'AATT_y
Cuts at: 1493 2483 3739 3965 4117 6226 1493
Size: 990 1256 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 990 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 3520 3755 1870
Size: 73 69 1508 235 4545
Fragments arranged by size:
4545 1508 235 73 69
BamHI G'GATC_C
Cuts at: 2145 3760 4127 2145
Size: 1615 367 4448
Fragments arranged by size:
4448 1615 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3456 3751 5206 618
Size: 325 217 627 1669 295 1455 1842
Fragments arranged by size:
1842 1669 1455 627 325 295 217
BanII G_rGCy'C
Cuts at: 1391 3443 3575 3616 3749 1391
Size: 2052 132 41 133 4072
Fragments arranged by size:
4072 2052 133 132 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5459
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4621 4655 1903
Size: 34 2684 34 3678
Fragments arranged by size:
3678 2684 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4322 5532 4322
Size: 1210 5220
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3493 5041 136
Size: 107 122 71 631 605 1821 1548 1525
Fragments arranged by size:
1821 1548 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2583 6425 2583
Size: 3842 2588
BlpI GC'TnA_GC
Cuts at: 3847 3847
Size: 6430
Bme1580I G_kGCm'C
Cuts at: 1790 2829 3666 4491 5647 1790
Size: 1039 837 825 1156 2573
Fragments arranged by size:
2573 1156 1039 837 825
BmgBI CAC'GTC
Cuts at: 1318 2161 1318
Size: 843 5587
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3021 3105 4161
Size: 416 53 83 399 24 1604 84 1056
Cuts at: 4161 5119 442
Size: 958 1753
Fragments arranged by size:
1753 1604 1056 958 416 399 84 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2255 2629 2949 3547 5090 1239
Size: 950 66 374 320 598 1543 2579
Fragments arranged by size:
2579 1543 950 598 374 320 66
Bpu10I CC'TnA_GC
Cuts at: 2202 2202
Size: 6430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2438 2477 4555 5420 5718 2438
Size: 39 2078 865 298 3150
Fragments arranged by size:
3150 2078 865 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2165 5093 882
Size: 617 666 2928 2219
Fragments arranged by size:
2928 2219 666 617
BsaAI yAC'GTr
Cuts at: 493 1204 4221 493
Size: 711 3017 2702
Fragments arranged by size:
3017 2702 711
BsaBI GATnn'nnATC
Cuts at: 6424 6424
Size: 6430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3311 4237 4619 5254
Size: 53 83 186 936 1778 926 382 635
Cuts at: 5254 275
Size: 1451
Fragments arranged by size:
1778 1451 936 926 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4863 5261 5535 5682 6093 4863
Size: 398 274 147 411 5200
Fragments arranged by size:
5200 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2174 2204 2380 2410 2588
Size: 30 165 30 771 30 176 30 178
Cuts at: 2588 2618 3047 3077 3545 3575 1178
Size: 30 429 30 468 30 4033
Fragments arranged by size:
4033 771 468 429 178 176 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2366 2887 3055 3197 3494 1186
Size: 1180 521 168 142 297 4122
Fragments arranged by size:
4122 1180 521 297 168 142
BseYI C'CCAG_C
Cuts at: 2229 3243 5633 6129 2229
Size: 1014 2390 496 2530
Fragments arranged by size:
2530 2390 1014 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2780 3146 1163
Size: 573 1044 366 4447
Fragments arranged by size:
4447 1044 573 366
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3880 4641 5669 1540
Size: 275 186 1879 761 1028 2301
Fragments arranged by size:
2301 1879 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2829 3443 3575 3666 4491 4576 5647 2829
Size: 614 132 91 825 85 1071 3612
Fragments arranged by size:
3612 1071 825 614 132 91 85
BsmI GAATG_Cn'
Cuts at: 3941 4034 3941
Size: 93 6337
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2829 3443 3575 3616 3666 3749 4491
Size: 399 1039 614 132 41 50 83 742
Cuts at: 4491 4576 5647 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1039 742 614 399 132 85
83 50 41
BspEI T'CCGG_A
Cuts at: 6093 6093
Size: 6430
BspHI T'CATG_A
Cuts at: 4319 6049 4319
Size: 1730 4700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2193 3122 3785 844
Size: 1349 929 663 3489
Fragments arranged by size:
3489 1349 929 663
BsrBI CCG'CTC
Cuts at: 1394 1823 4317 1394
Size: 429 2494 3507
Fragments arranged by size:
3507 2494 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4925 5099 65
Size: 1183 3677 174 1396
Fragments arranged by size:
3677 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2053 3501 5074 5261 1700
Size: 110 243 1448 1573 187 2869
Fragments arranged by size:
2869 1573 1448 243 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6430
BssSI C'ACGA_G
Cuts at: 4490 5502 4490
Size: 1012 5418
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6430
BstBI TT'CG_AA
Cuts at: 5234 5234
Size: 6430
BstEII G'GTnAC_C
Cuts at: 1184 2180 1184
Size: 996 5434
BstYI r'GATC_y
Cuts at: 1972 2145 2583 3760 4127 4512 4529 5981 6067
Size: 173 438 1177 367 385 17 1452 86
Cuts at: 6067 6079 6425 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1177 438 385 367 346 173
86 17 12
BstZ17I GTA'TAC
Cuts at: 2607 2607
Size: 6430
BtgI C'CryG_G
Cuts at: 513 1065 2004 3490 513
Size: 552 939 1486 3453
Fragments arranged by size:
3453 1486 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5691
Fragments arranged by size:
5691 683 56
BtsI GCAGTG_nn'
Cuts at: 3124 3205 3946 4740 4760 3124
Size: 81 741 794 20 4794
Fragments arranged by size:
4794 794 741 81 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2854 2889 529
Size: 35 2290 35 4070
Fragments arranged by size:
4070 2290 35 35
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5437 809
Size: 507 4121 1802
Fragments arranged by size:
4121 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3877 4766 6395
Size: 54 1425 50 275 18 2047 889 1629
Cuts at: 6395 8
Size: 43
Fragments arranged by size:
2047 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3877 1537
Size: 275 2065 4090
Fragments arranged by size:
4090 2065 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2328 2343 3675 4360 1353
Size: 975 15 1332 685 3423
Fragments arranged by size:
3423 1332 975 685 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3597 5017 5391 5537 387
Size: 1064 138 2008 1420 374 146 1280
Fragments arranged by size:
2008 1420 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3441 3573 3441
Size: 132 6298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2189 2238 2255 2367 2629 2869
Size: 393 419 138 49 17 112 262 240
Cuts at: 2869 2949 3205 3547 4493 5090 5877 6145 1239
Size: 80 256 342 946 597 787 268 1524
Fragments arranged by size:
1524 946 787 597 419 393 342 268
262 256 240 138 112 80 49 17
EcoNI CCTnn'n_nnAGG
Cuts at: 3523 3523
Size: 6430
EcoO109I rG'GnC_Cy
Cuts at: 2198 2338 3048 3189 2198
Size: 140 710 141 5439
Fragments arranged by size:
5439 710 141 140
EcoRI G'AATT_C
Cuts at: 3739 4117 3739
Size: 378 6052
FspI TGC'GCA
Cuts at: 4936 4936
Size: 6430
HaeII r_GCGC'y
Cuts at: 1678 5577 1678
Size: 3899 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2164 2196 2291 2323 2342
Size: 32 135 32 592 32 95 32 19
Cuts at: 2342 2374 2380 2412 2428 2460 3301 3333 5071
Size: 32 6 32 16 32 841 32 1738
Cuts at: 5071 5103 5145 5177 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1738 841 592 135 95 42 32
32 32 32 32 32 32 32 32
32 19 16 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3774 4020 669
Size: 988 281 1836 246 3079
Fragments arranged by size:
3079 1836 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6430
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KpnI G_GTAC'C
Cuts at: 3755 3755
Size: 6430
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 6306 6306
Size: 6430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2460 5544 5728 1305
Size: 1155 3084 184 2007
Fragments arranged by size:
3084 2007 1155 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4608
Fragments arranged by size:
4608 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4388 4747 4906 518
Size: 648 3222 359 159 2042
Fragments arranged by size:
3222 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2128 2233 3253 3846 4523 5671 5916 1606
Size: 522 105 1020 593 677 1148 245 2120
Fragments arranged by size:
2120 1148 1020 677 593 522 245 105
NaeI GCC'GGC
Cuts at: 2055 3503 2055
Size: 1448 4982
NcoI C'CATG_G
Cuts at: 513 1065 2004 3490 513
Size: 552 939 1486 3453
Fragments arranged by size:
3453 1486 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6430
NgoMIV G'CCGG_C
Cuts at: 2053 3501 2053
Size: 1448 4982
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 3877 3877
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 3788 5333 1132
Size: 97 2559 1545 2229
Fragments arranged by size:
2559 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2278 3070 3346 1348
Size: 930 792 276 4432
Fragments arranged by size:
4432 930 792 276
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2446 1108
Size: 1338 5092
PfoI T'CCnGG_A
Cuts at: 2085 2085
Size: 6430
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 6012 6044 4499
Size: 32 1481 32 4885
Fragments arranged by size:
4885 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2198 2198
Size: 6430
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 4000 4000
Size: 6430
PspXI vC'TCGA_Gb
Cuts at: 3520 3520
Size: 6430
PstI C_TGCA'G
Cuts at: 830 3119 3580 3782 830
Size: 2289 461 202 3478
Fragments arranged by size:
3478 2289 461 202
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
PvuII CAG'CTG
Cuts at: 2128 2233 2128
Size: 105 6325
SacI G_AGCT'C
Cuts at: 3443 3575 3443
Size: 132 6298
SalI G'TCGA_C
Cuts at: 1936 3772 1936
Size: 1836 4594
SapI GCTCTTCn'nnn_
Cuts at: 3675 3675
Size: 6430
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 4678 6390 4678
Size: 1712 4718
SexAI A'CCwGG_T
Cuts at: 3537 3537
Size: 6430
SfcI C'TryA_G
Cuts at: 826 1045 3115 3576 3778 4913 5594 5785 826
Size: 219 2070 461 202 1135 681 191 1471
Fragments arranged by size:
2070 1471 1135 681 461 219 202 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 2053 1700
Size: 353 6077
SmaI CCC'GGG
Cuts at: 1872 3757 1872
Size: 1885 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2453 2492 3520 4534 5435 5697
Size: 197 926 510 39 1028 1014 901 262
Cuts at: 5697 820
Size: 1553
Fragments arranged by size:
1553 1028 1014 926 901 510 262 197
39
SnaBI TAC'GTA
Cuts at: 493 4221 493
Size: 3728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 3788 1132
Size: 2656 3774
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6430
StyI C'CwwG_G
Cuts at: 513 1065 2004 3490 3869 513
Size: 552 939 1486 379 3074
Fragments arranged by size:
3074 1486 939 552 379
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2245 2261 2291 3683 4457 4474 4627
Size: 1255 665 16 30 1392 774 17 153
Cuts at: 4627 4812 5257 325
Size: 185 445 1498
Fragments arranged by size:
1498 1392 1255 774 665 445 185 153
30 17 16
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2780 2978 4676
Size: 274 80 33 51 1443 803 198 1698
Cuts at: 4676 6388 96
Size: 1712 138
Fragments arranged by size:
1712 1698 1443 803 274 198 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2174 2206 2699 2731 3218 3250 3545 3577 6390
Size: 32 493 32 487 32 295 32 2813
Cuts at: 6390 6422 2174
Size: 32 2182
Fragments arranged by size:
2813 2182 493 487 295 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6430
XbaI T'CTAG_A
Cuts at: 2588 3766 2588
Size: 1178 5252
XhoI C'TCGA_G
Cuts at: 1943 3520 1943
Size: 1577 4853
XmaI C'CCGG_G
Cuts at: 1870 3755 1870
Size: 1885 4545
XmnI GAAnn'nnTTC
Cuts at: 2882 2966 3134 3218 3428 4559 2882
Size: 84 168 84 210 1131 4753
Fragments arranged by size:
4753 1131 210 168 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4238 276
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI XcmI