Restriction map for
pFN21AB5061
(Circular) (Six-base) MAPSORT of: pFN21AB5061.seq Check: 3230 from: 1 to: 6121
pFN21AB5061 6121 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3268 3464 1937
Size: 1331 196 4594
Fragments arranged by size:
4594 1331 196
Acc65I G'GTAC_C
Cuts at: 3442 3442
Size: 6121
AclI AA'CG_TT
Cuts at: 1563 4248 4621 1563
Size: 2685 373 3063
Fragments arranged by size:
3063 2685 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4184 5568 5836 1632
Size: 2552 1384 268 1917
Fragments arranged by size:
2552 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 5020 5997 1203
Size: 3817 977 1327
Fragments arranged by size:
3817 1327 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 4850 4850
Size: 6121
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6121
AlwNI CAG_nnn'CTG
Cuts at: 2801 3205 3864 5436 2801
Size: 404 659 1572 3486
Fragments arranged by size:
3486 1572 659 404
ApaLI G'TGCA_C
Cuts at: 4178 5334 4178
Size: 1156 4965
ApoI r'AATT_y
Cuts at: 1493 2009 2382 3430 3656 3808 5917 1493
Size: 516 373 1048 226 152 2109 1697
Fragments arranged by size:
2109 1697 1048 516 373 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4675 160
Size: 872 3643 1606
Fragments arranged by size:
3643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 3446 1870
Size: 73 1503 4545
Fragments arranged by size:
4545 1503 73
AvrII C'CTAG_G
Cuts at: 3413 3413
Size: 6121
BamHI G'GATC_C
Cuts at: 3240 3334 3451 3818 3240
Size: 94 117 367 5543
Fragments arranged by size:
5543 367 117 94
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2900 3029 3442 4897 618
Size: 325 217 627 1113 129 413 1455 1842
Fragments arranged by size:
1842 1455 1113 627 413 325 217 129
BanII G_rGCy'C
Cuts at: 1391 3440 1391
Size: 2049 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4312 4346 1903
Size: 34 2375 34 3678
Fragments arranged by size:
3678 2375 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4013 5223 4013
Size: 1210 4911
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4732 136
Size: 107 122 71 631 605 3060 1525
Fragments arranged by size:
3060 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2959 6116 2959
Size: 3157 2964
BlpI GC'TnA_GC
Cuts at: 3538 3538
Size: 6121
Bme1580I G_kGCm'C
Cuts at: 1790 2685 4182 5338 1790
Size: 895 1497 1156 2573
Fragments arranged by size:
2573 1497 1156 895
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6121
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2350 2841 3126
Size: 416 53 83 399 24 933 491 285
Cuts at: 3126 3852 4810 442
Size: 726 958 1753
Fragments arranged by size:
1753 958 933 726 491 416 399 285
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 2339 2393 2415 3270 3279 4781 1239
Size: 1085 15 54 22 855 9 1502 2579
Fragments arranged by size:
2579 1502 1085 855 54 22 15 9
Bpu10I CC'TnA_GC
Cuts at: 3206 3206
Size: 6121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2530 4246 5111 5409 2530
Size: 1716 865 298 3242
Fragments arranged by size:
3242 1716 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4784 882
Size: 617 3285 2219
Fragments arranged by size:
3285 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3912 493
Size: 711 2708 2702
Fragments arranged by size:
2708 2702 711
BsaBI GATnn'nnATC
Cuts at: 2396 6115 2396
Size: 3719 2402
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3928 4310 4945 275
Size: 53 83 186 936 2395 382 635 1451
Fragments arranged by size:
2395 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4554 4952 5226 5373 5784 4554
Size: 398 274 147 411 4891
Fragments arranged by size:
4891 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5896
Fragments arranged by size:
5896 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2418 3176 1186
Size: 1232 758 4131
Fragments arranged by size:
4131 1232 758
BseYI C'CCAG_C
Cuts at: 2883 5324 5820 2883
Size: 2441 496 3184
Fragments arranged by size:
3184 2441 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5548
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3571 4332 5360 1540
Size: 275 186 1570 761 1028 2301
Fragments arranged by size:
2301 1570 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2060 3000 4182 4267 5338 2060
Size: 940 1182 85 1071 2843
Fragments arranged by size:
2843 1182 1071 940 85
BsmI GAATG_Cn'
Cuts at: 3632 3725 3632
Size: 93 6028
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2685 3000 3440 4182 4267 5338
Size: 399 270 625 315 440 742 85 1071
Cuts at: 5338 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 625 440 399 315 270
85
BspEI T'CCGG_A
Cuts at: 5784 5784
Size: 6121
BspHI T'CATG_A
Cuts at: 4010 5740 4010
Size: 1730 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2295 3476 844
Size: 1451 1181 3489
Fragments arranged by size:
3489 1451 1181
BsrBI CCG'CTC
Cuts at: 1394 1823 4008 1394
Size: 429 2185 3507
Fragments arranged by size:
3507 2185 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4616 4790 65
Size: 1183 3368 174 1396
Fragments arranged by size:
3368 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4765 4952 1700
Size: 110 2955 187 2869
Fragments arranged by size:
2955 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 4181 5193 4181
Size: 1012 5109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6121
BstBI TT'CG_AA
Cuts at: 4925 4925
Size: 6121
BstEII G'GTnAC_C
Cuts at: 1184 2461 1184
Size: 1277 4844
BstYI r'GATC_y
Cuts at: 1972 2189 2959 3240 3334 3451 3818 4203 4220
Size: 217 770 281 94 117 367 385 17
Cuts at: 4220 5672 5758 5770 6116 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 770 385 367 346 281 217
117 94 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2622 2904 3211 3310 513
Size: 552 1557 282 307 99 3324
Fragments arranged by size:
3324 1557 552 307 282 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5382
Fragments arranged by size:
5382 683 56
BtsI GCAGTG_nn'
Cuts at: 3142 3637 4431 4451 3142
Size: 495 794 20 4812
Fragments arranged by size:
4812 794 495 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 3425 3425
Size: 6121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3568 4457 6086
Size: 54 1425 50 275 18 1738 889 1629
Cuts at: 6086 8
Size: 43
Fragments arranged by size:
1738 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4051 1353
Size: 2698 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4708 5082 5228 387
Size: 1064 138 3119 374 146 1280
Fragments arranged by size:
3119 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2339 2393 2415 3270 3279 4184
Size: 393 692 15 54 22 855 9 905
Cuts at: 4184 4781 5568 5836 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 905 855 787 692 597 393 268
54 22 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2433 2652 2433
Size: 219 5902
EcoO109I rG'GnC_Cy
Cuts at: 2490 3202 2490
Size: 712 5409
EcoRI G'AATT_C
Cuts at: 2382 3430 3808 2382
Size: 1048 378 4695
Fragments arranged by size:
4695 1048 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2757 3310 2757
Size: 553 5568
FspI TGC'GCA
Cuts at: 4627 4627
Size: 6121
HaeII r_GCGC'y
Cuts at: 1678 5268 1678
Size: 3590 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2827 2859 4762 4794 4836
Size: 32 135 32 1255 32 1903 32 42
Cuts at: 4836 4868 1373
Size: 32 2626
Fragments arranged by size:
2626 1903 1255 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3465 3711 669
Size: 988 281 1527 246 3079
Fragments arranged by size:
3079 1527 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6121
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KpnI G_GTAC'C
Cuts at: 3446 3446
Size: 6121
MfeI C'AATT_G
Cuts at: 3720 3720
Size: 6121
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2206 2717 3093 5235 5419 1305
Size: 901 511 376 2142 184 2007
Fragments arranged by size:
2142 2007 901 511 376 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4299
Fragments arranged by size:
4299 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4079 4438 4597 518
Size: 648 2913 359 159 2042
Fragments arranged by size:
2913 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2130 2363 2883 3537 4214 5362 5607 1606
Size: 524 233 520 654 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 654 524 520 245 233
NcoI C'CATG_G
Cuts at: 513 1065 2904 3211 513
Size: 552 1839 307 3423
Fragments arranged by size:
3423 1839 552 307
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 3479 5024 1132
Size: 97 2250 1545 2229
Fragments arranged by size:
2250 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6121
PciI A'CATG_T
Cuts at: 5020 5020
Size: 6121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6121
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 5703 5735 4190
Size: 32 1481 32 4576
Fragments arranged by size:
4576 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3202 3202
Size: 6121
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6121
PsiI TTA'TAA
Cuts at: 2523 3691 2523
Size: 1168 4953
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2841 2873 2841
Size: 32 6089
PstI C_TGCA'G
Cuts at: 830 2292 2484 3010 3183 3473 830
Size: 1462 192 526 173 290 3478
Fragments arranged by size:
3478 1462 526 290 192 173
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
PvuII CAG'CTG
Cuts at: 2130 2363 2883 2130
Size: 233 520 5368
Fragments arranged by size:
5368 520 233
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SfcI C'TryA_G
Cuts at: 826 1045 2288 2480 2641 2817 3006 3073 3179
Size: 219 1243 192 161 176 189 67 106
Cuts at: 3179 3469 4604 5285 5476 826
Size: 290 1135 681 191 1471
Fragments arranged by size:
1471 1243 1135 681 290 219 192 191
189 176 161 106 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 3448 1872
Size: 1576 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2545 4225 5126 5388 820
Size: 197 926 602 1680 901 262 1553
Fragments arranged by size:
1680 1553 926 901 602 262 197
SnaBI TAC'GTA
Cuts at: 493 3912 493
Size: 3419 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 3479 1132
Size: 2347 3774
SspI AAT'ATT
Cuts at: 5 52 3064 4045 5
Size: 47 3012 981 2081
Fragments arranged by size:
3012 2081 981 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6121
StyI C'CwwG_G
Cuts at: 513 1065 2589 2904 3211 3413 3560 513
Size: 552 1524 315 307 202 147 3074
Fragments arranged by size:
3074 1524 552 315 307 202 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4148 4165 4318 4503 4948 325
Size: 1255 2568 17 153 185 445 1498
Fragments arranged by size:
2568 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2857 4367 6079
Size: 274 80 33 51 1443 880 1510 1712
Cuts at: 6079 96
Size: 138
Fragments arranged by size:
1712 1510 1443 880 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2033 2065 6081 6113 2033
Size: 32 4016 32 2041
Fragments arranged by size:
4016 2041 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6121
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3076 3154 3328 3076
Size: 78 174 5869
Fragments arranged by size:
5869 174 78
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 3446 1870
Size: 1576 4545
XmnI GAAnn'nnTTC
Cuts at: 2740 4250 2740
Size: 1510 4611
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI