Restriction map for
pFN21AB5057
(Circular) (Six-base) MAPSORT of: pFN21AB5057.seq Check: 4217 from: 1 to: 6304
pFN21AB5057 6304 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2080 2080
Size: 6304
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4114 278
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3647 1937
Size: 1710 4594
Acc65I G'GTAC_C
Cuts at: 3625 3625
Size: 6304
AclI AA'CG_TT
Cuts at: 1563 4431 4804 1563
Size: 2868 373 3063
Fragments arranged by size:
3063 2868 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3113 3346 3603 4367 5751 6019 1632
Size: 1481 233 257 764 1384 268 1917
Fragments arranged by size:
1917 1481 1384 764 268 257 233
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6107
AflIII A'CryG_T
Cuts at: 1203 2784 5203 6180 1203
Size: 1581 2419 977 1327
Fragments arranged by size:
2419 1581 1327 977
AgeI A'CCGG_T
Cuts at: 5135 5135
Size: 6304
AhdI GACnn_n'nnGTC
Cuts at: 5033 5033
Size: 6304
AleI CACnn'nnGTG
Cuts at: 1166 2177 2240 2856 1166
Size: 1011 63 616 4614
Fragments arranged by size:
4614 1011 616 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3407 1184
Size: 2223 4081
AlwNI CAG_nnn'CTG
Cuts at: 2575 4047 5619 2575
Size: 1472 1572 3260
Fragments arranged by size:
3260 1572 1472
ApaI G_GGCC'C
Cuts at: 2749 2749
Size: 6304
ApaLI G'TGCA_C
Cuts at: 2618 4361 5517 2618
Size: 1743 1156 3405
Fragments arranged by size:
3405 1743 1156
ApoI r'AATT_y
Cuts at: 1493 2451 3613 3839 3991 6100 1493
Size: 958 1162 226 152 2109 1697
Fragments arranged by size:
2109 1697 1162 958 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4858 160
Size: 872 3826 1606
Fragments arranged by size:
3826 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
AvaI C'yCGr_G
Cuts at: 1870 1943 3629 1870
Size: 73 1686 4545
Fragments arranged by size:
4545 1686 73
BamHI G'GATC_C
Cuts at: 3634 4001 3634
Size: 367 5937
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2732 2858 3290 3625 5080
Size: 325 217 627 945 126 432 335 1455
Cuts at: 5080 618
Size: 1842
Fragments arranged by size:
1842 1455 945 627 432 335 325 217
126
BanII G_rGCy'C
Cuts at: 1391 2749 3348 3623 1391
Size: 1358 599 275 4072
Fragments arranged by size:
4072 1358 599 275
BbeI G_GCGC'C
Cuts at: 2736 3294 2736
Size: 558 5746
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2519 3352 928
Size: 971 620 833 3880
Fragments arranged by size:
3880 971 833 620
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2632 2666 4495 4529 1903
Size: 34 695 34 1829 34 3678
Fragments arranged by size:
3678 1829 695 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4196 5406 4196
Size: 1210 5094
BclI T'GATC_A
Cuts at: 1548 2343 1548
Size: 795 5509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4915 136
Size: 107 122 71 631 605 3243 1525
Fragments arranged by size:
3243 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2067 2589 6299 2067
Size: 522 3710 2072
Fragments arranged by size:
3710 2072 522
BlpI GC'TnA_GC
Cuts at: 3183 3721 3183
Size: 538 5766
Bme1580I G_kGCm'C
Cuts at: 1790 2622 2710 2749 4365 5521 1790
Size: 832 88 39 1616 1156 2573
Fragments arranged by size:
2573 1616 1156 832 88 39
BmgBI CAC'GTC
Cuts at: 1318 2785 3526 1318
Size: 1467 741 4096
Fragments arranged by size:
4096 1467 741
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2668 4035 4993
Size: 416 53 83 399 24 1251 1367 958
Cuts at: 4993 442
Size: 1753
Fragments arranged by size:
1753 1367 1251 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2279 2603 4964 1239
Size: 959 81 324 2361 2579
Fragments arranged by size:
2579 2361 959 324 81
Bpu10I CC'TnA_GC
Cuts at: 2111 3113 2111
Size: 1002 5302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4429 5294 5592 4429
Size: 865 298 5141
Fragments arranged by size:
5141 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2554 3509 4967 882
Size: 617 1055 955 1458 2219
Fragments arranged by size:
2219 1458 1055 955 617
BsaAI yAC'GTr
Cuts at: 493 1204 4095 493
Size: 711 2891 2702
Fragments arranged by size:
2891 2702 711
BsaBI GATnn'nnATC
Cuts at: 6298 6298
Size: 6304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2733 3291 4111 4493
Size: 53 83 186 936 1200 558 820 382
Cuts at: 4493 5128 275
Size: 635 1451
Fragments arranged by size:
1451 1200 936 820 635 558 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3408 4737 5135 5409 5556 5967 3408
Size: 1329 398 274 147 411 3745
Fragments arranged by size:
3745 1329 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2706 2736 1178
Size: 30 165 30 1303 30 4746
Fragments arranged by size:
4746 1303 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2600 2616 3529 1186
Size: 949 465 16 913 3961
Fragments arranged by size:
3961 949 913 465 16
BseYI C'CCAG_C
Cuts at: 3044 3101 3551 5507 6003 3044
Size: 57 450 1956 496 3345
Fragments arranged by size:
3345 1956 496 450 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2760 3222 1163
Size: 573 1024 462 4245
Fragments arranged by size:
4245 1024 573 462
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3754 4515 5543 1540
Size: 275 186 1753 761 1028 2301
Fragments arranged by size:
2301 1753 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 2622 4365 4450 5521 2034
Size: 588 1743 85 1071 2817
Fragments arranged by size:
2817 1743 1071 588 85
BsmI GAATG_Cn'
Cuts at: 2959 3103 3269 3815 3908 2959
Size: 144 166 546 93 5355
Fragments arranged by size:
5355 546 166 144 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2622 2710 2749 3348 3623 4365
Size: 399 244 588 88 39 599 275 742
Cuts at: 4365 4450 5521 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 599 588 399 275 244
88 85 39
BspEI T'CCGG_A
Cuts at: 3408 5967 3408
Size: 2559 3745
BspHI T'CATG_A
Cuts at: 4193 5923 4193
Size: 1730 4574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2080 3399 3659 844
Size: 1236 1319 260 3489
Fragments arranged by size:
3489 1319 1236 260
BsrBI CCG'CTC
Cuts at: 1394 1823 2407 4191 1394
Size: 429 584 1784 3507
Fragments arranged by size:
3507 1784 584 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3438 4799 4973 65
Size: 1183 2190 1361 174 1396
Fragments arranged by size:
2190 1396 1361 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4948 5135 1700
Size: 110 3138 187 2869
Fragments arranged by size:
3138 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6304
BssSI C'ACGA_G
Cuts at: 4364 5376 4364
Size: 1012 5292
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6304
BstBI TT'CG_AA
Cuts at: 5108 5108
Size: 6304
BstEII G'GTnAC_C
Cuts at: 1184 2179 1184
Size: 995 5309
BstYI r'GATC_y
Cuts at: 1972 2067 2254 2589 3634 4001 4386 4403 5855
Size: 95 187 335 1045 367 385 17 1452
Cuts at: 5855 5941 5953 6299 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1045 385 367 346 335 187
95 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2710 2804 3276 513
Size: 552 939 706 94 472 3541
Fragments arranged by size:
3541 939 706 552 472 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5565
Fragments arranged by size:
5565 683 56
BtsI GCAGTG_nn'
Cuts at: 3820 4614 4634 3820
Size: 794 20 5490
Fragments arranged by size:
5490 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6269
DraI TTT'AAA
Cuts at: 3608 3608
Size: 6304
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5311 809
Size: 507 3995 1802
Fragments arranged by size:
3995 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2355 2758 3273
Size: 54 1425 50 275 18 525 403 515
Cuts at: 3273 3751 4640 6269 8
Size: 478 889 1629 43
Fragments arranged by size:
1629 1425 889 525 515 478 403 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3751 1537
Size: 275 1939 4090
Fragments arranged by size:
4090 1939 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2790 4234 1353
Size: 1437 1444 3423
Fragments arranged by size:
3423 1444 1437
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3176 4891 5265 5411 387
Size: 1064 138 1587 1715 374 146 1280
Fragments arranged by size:
1715 1587 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2279 2603 3113 3346 3603 4367
Size: 393 566 81 324 510 233 257 764
Cuts at: 4367 4964 5751 6019 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 764 597 566 510 393 324
268 257 233 81
EcoNI CCTnn'n_nnAGG
Cuts at: 3143 3461 3143
Size: 318 5986
EcoO109I rG'GnC_Cy
Cuts at: 2746 2962 3048 3388 2746
Size: 216 86 340 5662
Fragments arranged by size:
5662 340 216 86
EcoRI G'AATT_C
Cuts at: 2451 3613 3991 2451
Size: 1162 378 4764
Fragments arranged by size:
4764 1162 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 6304
FspI TGC'GCA
Cuts at: 4810 4810
Size: 6304
HaeII r_GCGC'y
Cuts at: 1678 2736 3294 5451 1678
Size: 1058 558 2157 2531
Fragments arranged by size:
2531 2157 1058 558
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2581 2613 4945 4977 5019
Size: 32 135 32 1009 32 2332 32 42
Cuts at: 5019 5051 1373
Size: 32 2626
Fragments arranged by size:
2626 2332 1009 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3648 3894 669
Size: 988 281 1710 246 3079
Fragments arranged by size:
3079 1710 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6304
HpaI GTT'AAC
Cuts at: 3894 3894
Size: 6304
KasI G'GCGC_C
Cuts at: 2732 3290 2732
Size: 558 5746
KpnI G_GTAC'C
Cuts at: 3629 3629
Size: 6304
MfeI C'AATT_G
Cuts at: 3903 3903
Size: 6304
MluI A'CGCG_T
Cuts at: 6180 6180
Size: 6304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5418 5602 1305
Size: 4113 184 2007
Fragments arranged by size:
4113 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2357 2760 10
Size: 54 1425 343 525 403 3554
Fragments arranged by size:
3554 1425 525 403 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2240 2615 2832 2856 3068 4262
Size: 648 1011 63 375 217 24 212 1194
Cuts at: 4262 4621 4780 518
Size: 359 159 2042
Fragments arranged by size:
2042 1194 1011 648 375 359 217 212
159 63 24
MspA1I CmG'CkG
Cuts at: 1606 2186 2204 2575 2844 2886 3720 4397 5545
Size: 580 18 371 269 42 834 677 1148
Cuts at: 5545 5790 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 834 677 580 371 269 245
42 18
NarI GG'CG_CC
Cuts at: 2733 3291 2733
Size: 558 5746
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4813
Fragments arranged by size:
4813 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6304
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6304
NotI GC'GGCC_GC
Cuts at: 3751 3751
Size: 6304
NspI r_CATG'y
Cuts at: 1132 1229 3578 3662 5207 1132
Size: 97 2349 84 1545 2229
Fragments arranged by size:
2349 2229 1545 97 84
PasI CC'CwG_GG
Cuts at: 1348 2446 1348
Size: 1098 5206
PciI A'CATG_T
Cuts at: 5203 5203
Size: 6304
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2221 2997 3307 1108
Size: 1113 776 310 4105
Fragments arranged by size:
4105 1113 776 310
PmeI GTTT'AAAC
Cuts at: 3608 3608
Size: 6304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4373 4405 5886 5918 4373
Size: 32 1481 32 4759
Fragments arranged by size:
4759 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3048 3388 3048
Size: 340 5964
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6304
PsiI TTA'TAA
Cuts at: 3874 3874
Size: 6304
PspOMI G'GGCC_C
Cuts at: 2745 2745
Size: 6304
PstI C_TGCA'G
Cuts at: 830 2091 2142 2754 3342 3656 830
Size: 1261 51 612 588 314 3478
Fragments arranged by size:
3478 1261 612 588 314 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6304
PvuII CAG'CTG
Cuts at: 2204 2575 2844 2204
Size: 371 269 5664
Fragments arranged by size:
5664 371 269
SalI G'TCGA_C
Cuts at: 1936 3646 1936
Size: 1710 4594
SanDI GG'GwC_CC
Cuts at: 3048 3048
Size: 6304
SapI GCTCTTCn'nnn_
Cuts at: 2790 2790
Size: 6304
SbfI CC_TGCA'GG
Cuts at: 3656 3656
Size: 6304
ScaI AGT'ACT
Cuts at: 4552 6264 4552
Size: 1712 4592
SfcI C'TryA_G
Cuts at: 826 1045 2087 2138 2517 2750 3338 3652 4787
Size: 219 1042 51 379 233 588 314 1135
Cuts at: 4787 5468 5659 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1042 681 588 379 314 233
219 191 51
SfoI GGC'GCC
Cuts at: 2734 3292 2734
Size: 558 5746
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6304
SmaI CCC'GGG
Cuts at: 1872 3631 1872
Size: 1759 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4408 5309 5571 820
Size: 197 926 2465 901 262 1553
Fragments arranged by size:
2465 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4095 493
Size: 3602 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6304
SphI G_CATG'C
Cuts at: 1132 3662 1132
Size: 2530 3774
SspI AAT'ATT
Cuts at: 5 52 4228 5
Size: 47 4176 2081
Fragments arranged by size:
4176 2081 47
StuI AGG'CCT
Cuts at: 1517 2154 1517
Size: 637 5667
StyI C'CwwG_G
Cuts at: 513 1065 2004 2175 2817 2868 2996 3319 3743
Size: 552 939 171 642 51 128 323 424
Cuts at: 3743 513
Size: 3074
Fragments arranged by size:
3074 939 642 552 424 323 171 128
51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2846 4331 4348 4501 4686 5131 325
Size: 1255 1266 1485 17 153 185 445 1498
Fragments arranged by size:
1498 1485 1266 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3334 4550 6262
Size: 274 80 33 51 1443 1357 1216 1712
Cuts at: 6262 96
Size: 138
Fragments arranged by size:
1712 1443 1357 1216 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2706 2738 6264 6296 2706
Size: 32 3526 32 2714
Fragments arranged by size:
3526 2714 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6304
XbaI T'CTAG_A
Cuts at: 3640 3640
Size: 6304
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6304
XmaI C'CCGG_G
Cuts at: 1870 3629 1870
Size: 1759 4545
XmnI GAAnn'nnTTC
Cuts at: 2414 4433 2414
Size: 2019 4285
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4112 276
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI
SrfI SwaI XcmI