Restriction map for
pFN21AB5050
(Circular) (Six-base) MAPSORT of: pFN21AB5050.seq Check: 4533 from: 1 to: 6586
pFN21AB5050 6586 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4396 278
Size: 53 83 186 936 2860 2468
Fragments arranged by size:
2860 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3124 3364 3929 1937
Size: 1187 240 565 4594
Fragments arranged by size:
4594 1187 565 240
Acc65I G'GTAC_C
Cuts at: 3907 3907
Size: 6586
AclI AA'CG_TT
Cuts at: 1563 4713 5086 1563
Size: 3150 373 3063
Fragments arranged by size:
3150 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 2730 3861 4649 6033 6301 1632
Size: 593 505 1131 788 1384 268 1917
Fragments arranged by size:
1917 1384 1131 788 593 505 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6389
AflIII A'CryG_T
Cuts at: 1203 2684 3247 3369 3489 5485 6462 1203
Size: 1481 563 122 120 1996 977 1327
Fragments arranged by size:
1996 1481 1327 977 563 122 120
AgeI A'CCGG_T
Cuts at: 5417 5417
Size: 6586
AhdI GACnn_n'nnGTC
Cuts at: 5315 5315
Size: 6586
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6586
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3654 1184
Size: 2470 4116
AlwNI CAG_nnn'CTG
Cuts at: 2167 3113 3353 4329 5901 2167
Size: 946 240 976 1572 2852
Fragments arranged by size:
2852 1572 976 946 240
ApaLI G'TGCA_C
Cuts at: 4643 5799 4643
Size: 1156 5430
ApoI r'AATT_y
Cuts at: 1493 2700 3728 3895 4121 4273 6382 1493
Size: 1207 1028 167 226 152 2109 1697
Fragments arranged by size:
2109 1697 1207 1028 226 167 152
AseI AT'TA_AT
Cuts at: 160 1032 5140 160
Size: 872 4108 1606
Fragments arranged by size:
4108 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6586
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 3198 3911 1870
Size: 73 71 1184 713 4545
Fragments arranged by size:
4545 1184 713 73 71
BamHI G'GATC_C
Cuts at: 3343 3916 4283 3343
Size: 573 367 5646
Fragments arranged by size:
5646 573 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3501 3907 5362 618
Size: 325 217 627 1714 406 1455 1842
Fragments arranged by size:
1842 1714 1455 627 406 325 217
BanII G_rGCy'C
Cuts at: 1391 3905 1391
Size: 2514 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2718 928
Size: 971 819 4796
Fragments arranged by size:
4796 971 819
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4777 4811 1903
Size: 34 2840 34 3678
Fragments arranged by size:
3678 2840 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3428 4478 5688 3428
Size: 1050 1210 4326
Fragments arranged by size:
4326 1210 1050
BclI T'GATC_A
Cuts at: 1548 2905 2913 1548
Size: 1357 8 5221
Fragments arranged by size:
5221 1357 8
BfrBI ATG'CAT
Cuts at: 3166 3166
Size: 6586
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5197 136
Size: 107 122 71 631 605 3525 1525
Fragments arranged by size:
3525 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6581 6581
Size: 6586
BlpI GC'TnA_GC
Cuts at: 4003 4003
Size: 6586
Bme1580I G_kGCm'C
Cuts at: 1790 2195 3504 4647 5803 1790
Size: 405 1309 1143 1156 2573
Fragments arranged by size:
2573 1309 1156 1143 405
BmgBI CAC'GTC
Cuts at: 1318 2100 1318
Size: 782 5804
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2364 2581 4317
Size: 416 53 83 399 24 947 217 1736
Cuts at: 4317 5275 442
Size: 958 1753
Fragments arranged by size:
1753 1736 958 947 416 399 217 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6586
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2317 2888 3453 2317
Size: 571 565 5450
Fragments arranged by size:
5450 571 565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 2515 2716 2746 2878 3135 5246 1239
Size: 1211 65 201 30 132 257 2111 2579
Fragments arranged by size:
2579 2111 1211 257 201 132 65 30
Bpu10I CC'TnA_GC
Cuts at: 2105 2105
Size: 6586
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4711 5576 5874 4711
Size: 865 298 5423
Fragments arranged by size:
5423 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5249 882
Size: 617 3750 2219
Fragments arranged by size:
3750 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4377 493
Size: 711 3173 2702
Fragments arranged by size:
3173 2702 711
BsaBI GATnn'nnATC
Cuts at: 6580 6580
Size: 6586
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2431 4393 4775 5410
Size: 53 83 186 936 898 1962 382 635
Cuts at: 5410 275
Size: 1451
Fragments arranged by size:
1962 1451 936 898 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5019 5417 5691 5838 6249 5019
Size: 398 274 147 411 5356
Fragments arranged by size:
5356 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6361
Fragments arranged by size:
6361 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2880 1186
Size: 1694 4892
BseYI C'CCAG_C
Cuts at: 5789 6285 5789
Size: 496 6090
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3837 1163
Size: 573 2101 3912
Fragments arranged by size:
3912 2101 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4036 4797 5825 1540
Size: 275 186 2035 761 1028 2301
Fragments arranged by size:
2301 2035 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4647 4732 5803 4647
Size: 85 1071 5430
Fragments arranged by size:
5430 1071 85
BsmI GAATG_Cn'
Cuts at: 3109 3483 3562 4097 4190 3109
Size: 374 79 535 93 5505
Fragments arranged by size:
5505 535 374 93 79
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 3504 3905 4647 4732 5803 1391
Size: 399 405 1309 401 742 85 1071 2174
Fragments arranged by size:
2174 1309 1071 742 405 401 399 85
BspEI T'CCGG_A
Cuts at: 6249 6249
Size: 6586
BspHI T'CATG_A
Cuts at: 3172 4475 6205 3172
Size: 1303 1730 3553
Fragments arranged by size:
3553 1730 1303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2673 3941 844
Size: 1829 1268 3489
Fragments arranged by size:
3489 1829 1268
BsrBI CCG'CTC
Cuts at: 1394 1823 2301 4473 1394
Size: 429 478 2172 3507
Fragments arranged by size:
3507 2172 478 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2609 2762 3611 5081 5255 65
Size: 1183 1361 153 849 1470 174 1396
Fragments arranged by size:
1470 1396 1361 1183 849 174 153
BsrFI r'CCGG_y
Cuts at: 1700 1810 2068 5230 5417 1700
Size: 110 258 3162 187 2869
Fragments arranged by size:
3162 2869 258 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6586
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6586
BssSI C'ACGA_G
Cuts at: 4646 5658 4646
Size: 1012 5574
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6586
BstBI TT'CG_AA
Cuts at: 3736 5390 3736
Size: 1654 4932
BstEII G'GTnAC_C
Cuts at: 1184 2936 1184
Size: 1752 4834
BstXI CCAn_nnnn'nTGG
Cuts at: 3856 3856
Size: 6586
BstYI r'GATC_y
Cuts at: 1972 3074 3343 3434 3916 4283 4668 4685 6137
Size: 1102 269 91 482 367 385 17 1452
Cuts at: 6137 6223 6235 6581 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1102 482 385 367 346 269
91 86 17 12
BstZ17I GTA'TAC
Cuts at: 3125 3125
Size: 6586
Bsu36I CC'TnA_GG
Cuts at: 2883 2883
Size: 6586
BtgI C'CryG_G
Cuts at: 513 1065 2284 2813 513
Size: 552 1219 529 4286
Fragments arranged by size:
4286 1219 552 529
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5847
Fragments arranged by size:
5847 683 56
BtsI GCAGTG_nn'
Cuts at: 4102 4896 4916 4102
Size: 794 20 5772
Fragments arranged by size:
5772 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6586
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2341 2376 529
Size: 35 1777 35 4739
Fragments arranged by size:
4739 1777 35 35
DraI TTT'AAA
Cuts at: 2973 3890 2973
Size: 917 5669
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5593 809
Size: 507 4277 1802
Fragments arranged by size:
4277 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2413 4033 4922
Size: 54 1425 50 275 18 583 1620 889
Cuts at: 4922 6551 8
Size: 1629 43
Fragments arranged by size:
1629 1620 1425 889 583 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4033 1537
Size: 275 2221 4090
Fragments arranged by size:
4090 2221 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2739 4516 1353
Size: 1386 1777 3423
Fragments arranged by size:
3423 1777 1386
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3799 5173 5547 5693 387
Size: 1064 138 2210 1374 374 146 1280
Fragments arranged by size:
2210 1374 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2450 2515 2716 2730 2746 2878
Size: 393 593 225 65 201 14 16 132
Cuts at: 2878 3135 3861 4649 5246 6033 6301 1239
Size: 257 726 788 597 787 268 1524
Fragments arranged by size:
1524 788 787 726 597 593 393 268
257 225 201 132 65 16 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2145 2145
Size: 6586
EcoO109I rG'GnC_Cy
Cuts at: 2395 3620 2395
Size: 1225 5361
EcoRI G'AATT_C
Cuts at: 3895 4273 3895
Size: 378 6208
EcoRV GAT'ATC
Cuts at: 3586 3586
Size: 6586
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2735 2735
Size: 6586
FspI TGC'GCA
Cuts at: 5092 5092
Size: 6586
HaeII r_GCGC'y
Cuts at: 1678 5733 1678
Size: 4055 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3045 3077 5227 5259 5301
Size: 32 135 32 1473 32 2150 32 42
Cuts at: 5301 5333 1373
Size: 32 2626
Fragments arranged by size:
2626 2150 1473 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3495 3513 3930 4176 669
Size: 988 281 1557 18 417 246 3079
Fragments arranged by size:
3079 1557 988 417 281 246 18
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6586
HpaI GTT'AAC
Cuts at: 3495 3513 4176 3495
Size: 18 663 5905
Fragments arranged by size:
5905 663 18
KpnI G_GTAC'C
Cuts at: 3911 3911
Size: 6586
MfeI C'AATT_G
Cuts at: 4185 4185
Size: 6586
MluI A'CGCG_T
Cuts at: 6462 6462
Size: 6586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2259 3809 5700 5884 1305
Size: 954 1550 1891 184 2007
Fragments arranged by size:
2007 1891 1550 954 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2415 10
Size: 54 1425 343 583 4181
Fragments arranged by size:
4181 1425 583 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3027 3171 3636 4544 4903 5062 518
Size: 648 1861 144 465 908 359 159 2042
Fragments arranged by size:
2042 1861 908 648 465 359 159 144
MspA1I CmG'CkG
Cuts at: 1606 3279 4002 4679 5827 6072 1606
Size: 1673 723 677 1148 245 2120
Fragments arranged by size:
2120 1673 1148 723 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2284 2813 513
Size: 552 1219 529 4286
Fragments arranged by size:
4286 1219 552 529
NdeI CA'TA_TG
Cuts at: 387 2565 3319 387
Size: 2178 754 3654
Fragments arranged by size:
3654 2178 754
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6586
NotI GC'GGCC_GC
Cuts at: 4033 4033
Size: 6586
NsiI A_TGCA'T
Cuts at: 3168 3168
Size: 6586
NspI r_CATG'y
Cuts at: 1132 1229 2113 2688 2922 3251 3944 5489 1132
Size: 97 884 575 234 329 693 1545 2229
Fragments arranged by size:
2229 1545 884 693 575 329 234 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6586
PciI A'CATG_T
Cuts at: 2684 3247 5485 2684
Size: 563 2238 3785
Fragments arranged by size:
3785 2238 563
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6586
PfoI T'CCnGG_A
Cuts at: 2466 2649 2466
Size: 183 6403
PmeI GTTT'AAAC
Cuts at: 3890 3890
Size: 6586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4655 4687 6168 6200 4655
Size: 32 1481 32 5041
Fragments arranged by size:
5041 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2395 3620 2395
Size: 1225 5361
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6586
PsiI TTA'TAA
Cuts at: 4156 4156
Size: 6586
PstI C_TGCA'G
Cuts at: 830 3938 830
Size: 3108 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6586
SalI G'TCGA_C
Cuts at: 1936 3928 1936
Size: 1992 4594
SbfI CC_TGCA'GG
Cuts at: 3938 3938
Size: 6586
ScaI AGT'ACT
Cuts at: 4834 6546 4834
Size: 1712 4874
SexAI A'CCwGG_T
Cuts at: 2794 2794
Size: 6586
SfcI C'TryA_G
Cuts at: 826 1045 2457 3078 3567 3934 5069 5750 5941
Size: 219 1412 621 489 367 1135 681 191
Cuts at: 5941 826
Size: 1471
Fragments arranged by size:
1471 1412 1135 681 621 489 367 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6586
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6586
SmaI CCC'GGG
Cuts at: 1872 3913 1872
Size: 2041 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3198 4690 5591 5853 820
Size: 197 926 1255 1492 901 262 1553
Fragments arranged by size:
1553 1492 1255 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4377 493
Size: 3884 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6586
SphI G_CATG'C
Cuts at: 1132 3944 1132
Size: 2812 3774
SspI AAT'ATT
Cuts at: 5 52 3541 4510 5
Size: 47 3489 969 2081
Fragments arranged by size:
3489 2081 969 47
StuI AGG'CCT
Cuts at: 1517 2463 1517
Size: 946 5640
StyI C'CwwG_G
Cuts at: 513 1065 2284 2416 2813 4025 513
Size: 552 1219 132 397 1212 3074
Fragments arranged by size:
3074 1219 1212 552 397 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4613 4630 4783 4968 5413 325
Size: 1255 3033 17 153 185 445 1498
Fragments arranged by size:
3033 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4832 6544 96
Size: 274 80 33 51 1443 2855 1712 138
Fragments arranged by size:
2855 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6546 6578 6546
Size: 32 6554
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6586
XbaI T'CTAG_A
Cuts at: 3922 3922
Size: 6586
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3428 3428
Size: 6586
XhoI C'TCGA_G
Cuts at: 1943 3198 1943
Size: 1255 5331
XmaI C'CCGG_G
Cuts at: 1870 3911 1870
Size: 2041 4545
XmnI GAAnn'nnTTC
Cuts at: 3732 4715 3732
Size: 983 5603
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4394 276
Size: 53 83 186 936 2860 2468
Fragments arranged by size:
2860 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI