Restriction map for
pFN21AB5048
(Circular) (Six-base) MAPSORT of: pFN21AB5048.seq Check: 5821 from: 1 to: 6877
pFN21AB5048 6877 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2152 2234 3939 2152
Size: 82 1705 5090
Fragments arranged by size:
5090 1705 82
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2673 4687 278
Size: 53 83 186 936 1137 2014 2468
Fragments arranged by size:
2468 2014 1137 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2721 4220 1937
Size: 784 1499 4594
Fragments arranged by size:
4594 1499 784
Acc65I G'GTAC_C
Cuts at: 4198 4198
Size: 6877
AclI AA'CG_TT
Cuts at: 1563 5004 5377 1563
Size: 3441 373 3063
Fragments arranged by size:
3441 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2218 2503 2720 3161 4940 6324 6592 1632
Size: 586 285 217 441 1779 1384 268 1917
Fragments arranged by size:
1917 1779 1384 586 441 285 268 217
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6680
AflIII A'CryG_T
Cuts at: 1203 2706 5776 6753 1203
Size: 1503 3070 977 1327
Fragments arranged by size:
3070 1503 1327 977
AgeI A'CCGG_T
Cuts at: 5708 5708
Size: 6877
AhdI GACnn_n'nnGTC
Cuts at: 5606 5606
Size: 6877
AleI CACnn'nnGTG
Cuts at: 1166 2268 1166
Size: 1102 5775
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2700 1184
Size: 1516 5361
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3675 3707 4037 4069 3675
Size: 32 330 32 6483
Fragments arranged by size:
6483 330 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2806 3082 3263 3358 4620 6192 2806
Size: 276 181 95 1262 1572 3491
Fragments arranged by size:
3491 1572 1262 276 181 95
ApaI G_GGCC'C
Cuts at: 3041 3310 3630 3041
Size: 269 320 6288
Fragments arranged by size:
6288 320 269
ApaLI G'TGCA_C
Cuts at: 3596 4934 6090 3596
Size: 1338 1156 4383
Fragments arranged by size:
4383 1338 1156
ApoI r'AATT_y
Cuts at: 1493 4186 4412 4564 6673 1493
Size: 2693 226 152 2109 1697
Fragments arranged by size:
2693 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5431 160
Size: 872 4399 1606
Fragments arranged by size:
4399 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6877
AvaI C'yCGr_G
Cuts at: 1870 1943 2909 2928 3759 4164 4202 1870
Size: 73 966 19 831 405 38 4545
Fragments arranged by size:
4545 966 831 405 73 38 19
BamHI G'GATC_C
Cuts at: 3683 4207 4574 3683
Size: 524 367 5986
Fragments arranged by size:
5986 524 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2840 4198 5653 618
Size: 325 217 627 1053 1358 1455 1842
Fragments arranged by size:
1842 1455 1358 1053 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2963 3041 3257 3310 3530 3630 4196 1391
Size: 1572 78 216 53 220 100 566 4072
Fragments arranged by size:
4072 1572 566 220 216 100 78 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 2925 3561 928
Size: 971 162 864 636 4244
Fragments arranged by size:
4244 971 864 636 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2579 2613 3587 3621 4006 4040 5068
Size: 34 642 34 974 34 385 34 1028
Cuts at: 5068 5102 1903
Size: 34 3678
Fragments arranged by size:
3678 1028 974 642 385 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4769 5979 4769
Size: 1210 5667
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6877
BfrBI ATG'CAT
Cuts at: 2677 2677
Size: 6877
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5488 136
Size: 107 122 71 631 605 3816 1525
Fragments arranged by size:
3816 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4101 6872 4101
Size: 2771 4106
BlpI GC'TnA_GC
Cuts at: 4294 4294
Size: 6877
Bme1580I G_kGCm'C
Cuts at: 1790 2843 3041 3310 3600 3630 4938 6094 1790
Size: 1053 198 269 290 30 1308 1156 2573
Fragments arranged by size:
2573 1308 1156 1053 290 269 198 30
BmgBI CAC'GTC
Cuts at: 1318 2377 1318
Size: 1059 5818
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2108 3532 4608
Size: 416 53 83 399 24 691 1424 1076
Cuts at: 4608 5566 442
Size: 958 1753
Fragments arranged by size:
1753 1424 1076 958 691 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6877
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 4084 4153 4190 2443
Size: 1641 69 37 5130
Fragments arranged by size:
5130 1641 69 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2874 3514 3989 4064 5537 1239
Size: 851 784 640 475 75 1473 2579
Fragments arranged by size:
2579 1473 851 784 640 475 75
Bpu10I CC'TnA_GC
Cuts at: 3256 3347 3359 3256
Size: 91 12 6774
Fragments arranged by size:
6774 91 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5002 5867 6165 5002
Size: 865 298 5714
Fragments arranged by size:
5714 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3440 5540 882
Size: 617 1941 2100 2219
Fragments arranged by size:
2219 2100 1941 617
BsaAI yAC'GTr
Cuts at: 493 1204 2709 4668 493
Size: 711 1505 1959 2702
Fragments arranged by size:
2702 1959 1505 711
BsaBI GATnn'nnATC
Cuts at: 6871 6871
Size: 6877
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2670 4684 5066 5701
Size: 53 83 186 936 1137 2014 382 635
Cuts at: 5701 275
Size: 1451
Fragments arranged by size:
2014 1451 1137 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5310 5708 5982 6129 6540 5310
Size: 398 274 147 411 5647
Fragments arranged by size:
5647 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2945 2975 3512 3542 4037
Size: 30 165 30 1542 30 537 30 495
Cuts at: 4037 4067 1178
Size: 30 3988
Fragments arranged by size:
3988 1542 537 495 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3422 4019 4112 1186
Size: 2236 597 93 3951
Fragments arranged by size:
3951 2236 597 93
BseYI C'CCAG_C
Cuts at: 2741 2799 3470 6080 6576 2741
Size: 58 671 2610 496 3042
Fragments arranged by size:
3042 2610 671 496 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3917 1163
Size: 573 2181 4123
Fragments arranged by size:
4123 2181 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4327 5088 6116 1540
Size: 275 186 2326 761 1028 2301
Fragments arranged by size:
2326 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 3600 4938 5023 6094 2076
Size: 1524 1338 85 1071 2859
Fragments arranged by size:
2859 1524 1338 1071 85
BsmI GAATG_Cn'
Cuts at: 3151 4388 4481 3151
Size: 1237 93 5547
Fragments arranged by size:
5547 1237 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2843 2963 3041 3257 3310 3530
Size: 399 286 767 120 78 216 53 220
Cuts at: 3530 3600 3630 4196 4938 5023 6094 1391
Size: 70 30 566 742 85 1071 2174
Fragments arranged by size:
2174 1071 767 742 566 399 286 220
216 120 85 78 70 53 30
BspEI T'CCGG_A
Cuts at: 6540 6540
Size: 6877
BspHI T'CATG_A
Cuts at: 3237 4766 6496 3237
Size: 1529 1730 3618
Fragments arranged by size:
3618 1730 1529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2152 2234 2913 3939 4232 844
Size: 1308 82 679 1026 293 3489
Fragments arranged by size:
3489 1308 1026 679 293 82
BsrBI CCG'CTC
Cuts at: 1394 1823 3963 4764 1394
Size: 429 2140 801 3507
Fragments arranged by size:
3507 2140 801 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2752 3132 3168 5372 5546 65
Size: 1183 1504 380 36 2204 174 1396
Fragments arranged by size:
2204 1504 1396 1183 380 174 36
BsrFI r'CCGG_y
Cuts at: 1700 1810 3806 5521 5708 1700
Size: 110 1996 1715 187 2869
Fragments arranged by size:
2869 1996 1715 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6877
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6877
BssSI C'ACGA_G
Cuts at: 4937 5949 4937
Size: 1012 5865
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6877
BstBI TT'CG_AA
Cuts at: 5681 5681
Size: 6877
BstEII G'GTnAC_C
Cuts at: 1184 2386 1184
Size: 1202 5675
BstXI CCAn_nnnn'nTGG
Cuts at: 2612 2971 3830 4042 2612
Size: 359 859 212 5447
Fragments arranged by size:
5447 859 359 212
BstYI r'GATC_y
Cuts at: 1972 2637 3078 3213 3683 4101 4207 4574 4959
Size: 665 441 135 470 418 106 367 385
Cuts at: 4959 4976 6428 6514 6526 6872 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 665 470 441 418 385 367
346 135 106 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2336 2336
Size: 6877
BtgI C'CryG_G
Cuts at: 513 1065 2004 2964 513
Size: 552 939 960 4426
Fragments arranged by size:
4426 960 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2224 2509 3925 487
Size: 683 56 998 285 1416 3439
Fragments arranged by size:
3439 1416 998 683 285 56
BtsI GCAGTG_nn'
Cuts at: 3877 4393 5187 5207 3877
Size: 516 794 20 5547
Fragments arranged by size:
5547 794 516 20
ClaI AT'CG_AT
Cuts at: 1554 3393 1554
Size: 1839 5038
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6842
DraI TTT'AAA
Cuts at: 4181 4181
Size: 6877
DraIII CAC_nnn'GTG
Cuts at: 3751 4062 4114 3751
Size: 311 52 6514
Fragments arranged by size:
6514 311 52
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5884 809
Size: 507 4568 1802
Fragments arranged by size:
4568 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2329 2733 2895
Size: 54 1425 50 275 18 499 404 162
Cuts at: 2895 3030 3733 3808 3897 4324 5213 6842 8
Size: 135 703 75 89 427 889 1629 43
Fragments arranged by size:
1629 1425 889 703 499 427 404 275
162 135 89 75 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4324 1537
Size: 275 2512 4090
Fragments arranged by size:
4090 2512 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3183 4807 1353
Size: 1830 1624 3423
Fragments arranged by size:
3423 1830 1624
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3944 5464 5838 5984 387
Size: 1064 138 2355 1520 374 146 1280
Fragments arranged by size:
2355 1520 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2218 2503 2720 2874 3161 3514
Size: 393 458 128 285 217 154 287 353
Cuts at: 3514 3989 4064 4940 5537 6324 6592 1239
Size: 475 75 876 597 787 268 1524
Fragments arranged by size:
1524 876 787 597 475 458 393 353
287 285 268 217 154 128 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2417 3350 2417
Size: 933 5944
EcoO109I rG'GnC_Cy
Cuts at: 2501 2902 3037 3038 3306 3307 3355 4132 4142
Size: 401 135 1 268 1 48 777 10
Cuts at: 4142 2501
Size: 5236
Fragments arranged by size:
5236 777 401 268 135 48 10 1
1
EcoRI G'AATT_C
Cuts at: 4186 4564 4186
Size: 378 6499
FseI GG_CCGG'CC
Cuts at: 3810 3810
Size: 6877
FspI TGC'GCA
Cuts at: 5383 5383
Size: 6877
HaeII r_GCGC'y
Cuts at: 1678 2884 6024 1678
Size: 1206 3140 2531
Fragments arranged by size:
3140 2531 1206
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 2622 2654 3157
Size: 32 135 32 692 32 326 32 503
Cuts at: 3157 3189 3512 3544 3644 3676 5518 5550 5592
Size: 32 323 32 100 32 1842 32 42
Cuts at: 5592 5624 1373
Size: 32 2626
Fragments arranged by size:
2626 1842 692 503 326 323 135 100
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2125 2344 2650 3091 4221 4467
Size: 988 281 187 219 306 441 1130 246
Cuts at: 4467 669
Size: 3079
Fragments arranged by size:
3079 1130 988 441 306 281 246 219
187
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6877
HpaI GTT'AAC
Cuts at: 4467 4467
Size: 6877
KpnI G_GTAC'C
Cuts at: 4202 4202
Size: 6877
MfeI C'AATT_G
Cuts at: 4476 4476
Size: 6877
MluI A'CGCG_T
Cuts at: 6753 6753
Size: 6877
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5991 6175 1305
Size: 4686 184 2007
Fragments arranged by size:
4686 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2735 2897 10
Size: 54 1425 343 903 162 3990
Fragments arranged by size:
3990 1425 903 343 162 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2268 2610 2682 2858 3828 4835 5194
Size: 648 1102 342 72 176 970 1007 359
Cuts at: 5194 5353 518
Size: 159 2042
Fragments arranged by size:
2042 1102 1007 970 648 359 342 176
159 72
MspA1I CmG'CkG
Cuts at: 1606 2399 4293 4970 6118 6363 1606
Size: 793 1894 677 1148 245 2120
Fragments arranged by size:
2120 1894 1148 793 677 245
NaeI GCC'GGC
Cuts at: 3808 3808
Size: 6877
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5386
Fragments arranged by size:
5386 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6877
NgoMIV G'CCGG_C
Cuts at: 3806 3806
Size: 6877
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6877
NotI GC'GGCC_GC
Cuts at: 4324 4324
Size: 6877
NsiI A_TGCA'T
Cuts at: 2679 2679
Size: 6877
NspI r_CATG'y
Cuts at: 1132 1229 3389 4235 5780 1132
Size: 97 2160 846 1545 2229
Fragments arranged by size:
2229 2160 1545 846 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6877
PciI A'CATG_T
Cuts at: 5776 5776
Size: 6877
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2806 3522 1108
Size: 1698 716 4463
Fragments arranged by size:
4463 1698 716
PmeI GTTT'AAAC
Cuts at: 4181 4181
Size: 6877
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 4946 4978 6459 6491 4037
Size: 32 877 32 1481 32 4423
Fragments arranged by size:
4423 1481 877 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2902 4132 2902
Size: 1230 5647
PshAI GACnn'nnGTC
Cuts at: 1613 3839 1613
Size: 2226 4651
PsiI TTA'TAA
Cuts at: 4447 4447
Size: 6877
PspOMI G'GGCC_C
Cuts at: 3037 3306 3626 3037
Size: 269 320 6288
Fragments arranged by size:
6288 320 269
PstI C_TGCA'G
Cuts at: 830 3046 3921 4229 830
Size: 2216 875 308 3478
Fragments arranged by size:
3478 2216 875 308
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6877
SalI G'TCGA_C
Cuts at: 1936 4219 1936
Size: 2283 4594
SbfI CC_TGCA'GG
Cuts at: 4229 4229
Size: 6877
ScaI AGT'ACT
Cuts at: 5125 6837 5125
Size: 1712 5165
SfcI C'TryA_G
Cuts at: 826 1045 3042 3917 4225 5360 6041 6232 826
Size: 219 1997 875 308 1135 681 191 1471
Fragments arranged by size:
1997 1471 1135 875 681 308 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6877
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6877
SmaI CCC'GGG
Cuts at: 1872 3761 4204 1872
Size: 1889 443 4545
Fragments arranged by size:
4545 1889 443
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4981 5882 6144 820
Size: 197 926 3038 901 262 1553
Fragments arranged by size:
3038 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4668 493
Size: 4175 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6877
SphI G_CATG'C
Cuts at: 1132 4235 1132
Size: 3103 3774
SspI AAT'ATT
Cuts at: 5 52 4801 5
Size: 47 4749 2081
Fragments arranged by size:
4749 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6877
StyI C'CwwG_G
Cuts at: 513 1065 2004 2898 3330 3516 3622 3633 4316
Size: 552 939 894 432 186 106 11 683
Cuts at: 4316 513
Size: 3074
Fragments arranged by size:
3074 939 894 683 552 432 186 106
11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 2978 3076 3464 4904 4921 5074
Size: 1255 609 789 98 388 1440 17 153
Cuts at: 5074 5259 5704 325
Size: 185 445 1498
Fragments arranged by size:
1498 1440 1255 789 609 445 388 185
153 98 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3192 3813 5123
Size: 274 80 33 51 1443 1215 621 1310
Cuts at: 5123 6835 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1310 1215 621 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2945 2977 3414 3446 6837 6869 2945
Size: 32 437 32 3391 32 2953
Fragments arranged by size:
3391 2953 437 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3265 1316
Size: 1949 4928
XbaI T'CTAG_A
Cuts at: 4213 4213
Size: 6877
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6877
XmaI C'CCGG_G
Cuts at: 1870 3759 4202 1870
Size: 1889 443 4545
Fragments arranged by size:
4545 1889 443
XmnI GAAnn'nnTTC
Cuts at: 2922 5006 2922
Size: 2084 4793
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2671 4685 276
Size: 53 83 186 936 1137 2014 2468
Fragments arranged by size:
2468 2014 1137 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FalI FspAI KasI NarI NruI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI