Restriction map for
pFN21AB5034
(Circular) (Six-base) MAPSORT of: pFN21AB5034.seq Check: 9122 from: 1 to: 5653
pFN21AB5034 5653 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2365 2365
Size: 5653
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2037 2996 1937
Size: 100 959 4594
Fragments arranged by size:
4594 959 100
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 2525 3716 5100 5368 1632
Size: 503 390 1191 1384 268 1917
Fragments arranged by size:
1917 1384 1191 503 390 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 4552 5529 1203
Size: 3349 977 1327
Fragments arranged by size:
3349 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2744 2776 2744
Size: 32 5621
AlwNI CAG_nnn'CTG
Cuts at: 2503 2847 3396 4968 2503
Size: 344 549 1572 3188
Fragments arranged by size:
3188 1572 549 344
ApaI G_GGCC'C
Cuts at: 2068 2068
Size: 5653
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2962 3188 3340 5449 1493
Size: 1469 226 152 2109 1697
Fragments arranged by size:
2109 1697 1469 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2246 2488 2978 1870
Size: 73 303 242 490 4545
Fragments arranged by size:
4545 490 303 242 73
BamHI G'GATC_C
Cuts at: 2415 2983 3350 2415
Size: 568 367 4718
Fragments arranged by size:
4718 568 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2828 2974 4429 618
Size: 325 217 627 1041 146 1455 1842
Fragments arranged by size:
1842 1455 1041 627 325 217 146
BanII G_rGCy'C
Cuts at: 1391 2068 2112 2972 1391
Size: 677 44 860 4072
Fragments arranged by size:
4072 860 677 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2651 928
Size: 971 752 3930
Fragments arranged by size:
3930 971 752
BbvCI CC'TCA_GC
Cuts at: 2876 2876
Size: 5653
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BfrBI ATG'CAT
Cuts at: 2462 2462
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2219 2678 4264
Size: 107 122 71 631 605 547 459 1586
Cuts at: 4264 136
Size: 1525
Fragments arranged by size:
1586 1525 631 605 547 459 122 107
71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2068 2387 2833 3714 4870 1790
Size: 278 319 446 881 1156 2573
Fragments arranged by size:
2573 1156 881 446 319 278
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2152 2374 2551
Size: 416 53 83 399 24 735 222 177
Cuts at: 2551 3384 4342 442
Size: 833 958 1753
Fragments arranged by size:
1753 958 833 735 416 399 222 177
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2855 2876 4313 1239
Size: 1616 21 1437 2579
Fragments arranged by size:
2579 1616 1437 21
Bpu10I CC'TnA_GC
Cuts at: 2876 2876
Size: 5653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3778 4643 4941 3778
Size: 865 298 4490
Fragments arranged by size:
4490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2738 4316 882
Size: 617 1239 1578 2219
Fragments arranged by size:
2219 1578 1239 617
BsaAI yAC'GTr
Cuts at: 493 1204 2407 3444 493
Size: 711 1203 1037 2702
Fragments arranged by size:
2702 1203 1037 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2316 4086 4484 4758 4905 5316 2316
Size: 1770 398 274 147 411 2653
Fragments arranged by size:
2653 1770 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2517 2547 2744 2774 1178
Size: 30 165 30 1114 30 197 30 4057
Fragments arranged by size:
4057 1114 197 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2264 2384 2681 1186
Size: 1078 120 297 4158
Fragments arranged by size:
4158 1078 297 120
BseYI C'CCAG_C
Cuts at: 2517 2537 2589 2885 4856 5352 2517
Size: 20 52 296 1971 496 2818
Fragments arranged by size:
2818 1971 496 296 52 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 2399 1163
Size: 573 561 102 4417
Fragments arranged by size:
4417 573 561 102
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2143 3103 3864 4892 1540
Size: 275 186 142 960 761 1028 2301
Fragments arranged by size:
2301 1028 960 761 275 186 142
BsiHKAI G_wGCw'C
Cuts at: 2112 2527 3714 3799 4870 2112
Size: 415 1187 85 1071 2895
Fragments arranged by size:
2895 1187 1071 415 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2112 2387 2527 2833 2972 3714
Size: 399 278 44 275 140 306 139 742
Cuts at: 3714 3799 4870 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 306 278 275 140
139 85 44
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2365 3008 844
Size: 1521 643 3489
Fragments arranged by size:
3489 1521 643
BsrBI CCG'CTC
Cuts at: 1394 1823 2413 3540 1394
Size: 429 590 1127 3507
Fragments arranged by size:
3507 1127 590 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 4322 65
Size: 1183 2900 174 1396
Fragments arranged by size:
2900 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2723 4297 4484 1700
Size: 110 913 1574 187 2869
Fragments arranged by size:
2869 1574 913 187 110
BsrGI T'GTAC_A
Cuts at: 96 2767 96
Size: 2671 2982
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstXI CCAn_nnnn'nTGG
Cuts at: 2341 2341
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2415 2482 2983 3350 3735 3752 5204 5290
Size: 443 67 501 367 385 17 1452 86
Cuts at: 5290 5302 5648 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 501 443 385 367 346 86
67 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2217 2304 513
Size: 552 1152 87 3862
Fragments arranged by size:
3862 1152 552 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 2387 3169 3963 3983 2387
Size: 782 794 20 4057
Fragments arranged by size:
4057 794 782 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2736 2904
Size: 54 1425 50 275 18 381 525 168
Cuts at: 2904 3100 3989 5618 8
Size: 196 889 1629 43
Fragments arranged by size:
1629 1425 889 525 381 275 196 168
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3583 1353
Size: 2230 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2110 2110
Size: 5653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2525 2855 2876 3716 4313 5100
Size: 393 503 390 330 21 840 597 787
Cuts at: 5100 5368 1239
Size: 268 1524
Fragments arranged by size:
1524 840 787 597 503 393 390 330
268 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2072 2249 2072
Size: 177 5476
EcoO109I rG'GnC_Cy
Cuts at: 2064 2065 2553 2064
Size: 1 488 5164
Fragments arranged by size:
5164 488 1
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 5653
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 2330 4800 1678
Size: 652 2470 2531
Fragments arranged by size:
2531 2470 652
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4294 4326 4368 4400 1373
Size: 32 135 32 2722 32 42 32 2626
Fragments arranged by size:
2722 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4767 4951 1305
Size: 3462 184 2007
Fragments arranged by size:
3462 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2213 2738 10
Size: 54 1425 343 381 525 2925
Fragments arranged by size:
2925 1425 525 381 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3611 3970 4129 518
Size: 648 2445 359 159 2042
Fragments arranged by size:
2445 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2847 2909 3069 3746 4894 5139 1606
Size: 406 835 62 160 677 1148 245 2120
Fragments arranged by size:
2120 1148 835 677 406 245 160 62
NaeI GCC'GGC
Cuts at: 2725 2725
Size: 5653
NcoI C'CATG_G
Cuts at: 513 1065 2304 513
Size: 552 1239 3862
Fragments arranged by size:
3862 1239 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NgoMIV G'CCGG_C
Cuts at: 2723 2723
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NsiI A_TGCA'T
Cuts at: 2464 2464
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 2698 2791 3011 4556 1132
Size: 97 1469 93 220 1545 2229
Fragments arranged by size:
2229 1545 1469 220 97 93
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5653
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2744 2776 3722 3754 5235 5267 2744
Size: 32 946 32 1481 32 3130
Fragments arranged by size:
3130 1481 946 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2553 2553
Size: 5653
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PspOMI G'GGCC_C
Cuts at: 2064 2064
Size: 5653
PspXI vC'TCGA_Gb
Cuts at: 2246 2246
Size: 5653
PstI C_TGCA'G
Cuts at: 830 3005 830
Size: 2175 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2847 2847
Size: 5653
SacI G_AGCT'C
Cuts at: 2112 2112
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SanDI GG'GwC_CC
Cuts at: 2553 2553
Size: 5653
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 3001 4136 4817 5008 826
Size: 219 1956 1135 681 191 1471
Fragments arranged by size:
1956 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2246 3757 4658 4920 820
Size: 197 926 303 1511 901 262 1553
Fragments arranged by size:
1553 1511 926 901 303 262 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2127 2304 2334 2388 2748 3092 513
Size: 552 1062 177 30 54 360 344 3074
Fragments arranged by size:
3074 1062 552 360 344 177 54 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2171 3680 3697 3850 4035 4480 325
Size: 1255 591 1509 17 153 185 445 1498
Fragments arranged by size:
1509 1498 1255 591 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2295 2767 3899
Size: 274 80 33 51 1443 318 472 1132
Cuts at: 3899 5611 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1132 472 318 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2517 2549 5613 5645 2517
Size: 32 3064 32 2525
Fragments arranged by size:
3064 2525 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2746 2746
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 2246 1943
Size: 303 5350
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI
NruI PacI PfoI PmlI PsrI RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI