Restriction map for
pFN21AB5026
(Circular) (Six-base) MAPSORT of: pFN21AB5026.seq Check: 3381 from: 1 to: 6049
pFN21AB5026 6049 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3859 278
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2544 3392 1937
Size: 607 848 4594
Fragments arranged by size:
4594 848 607
Acc65I G'GTAC_C
Cuts at: 3370 3370
Size: 6049
AclI AA'CG_TT
Cuts at: 1563 4176 4549 1563
Size: 2613 373 3063
Fragments arranged by size:
3063 2613 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2110 2184 2384 2603 3255 4112 5496 5764
Size: 478 74 200 219 652 857 1384 268
Cuts at: 5764 1632
Size: 1917
Fragments arranged by size:
1917 1384 857 652 478 268 219 200
74
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5852
AflIII A'CryG_T
Cuts at: 1203 2776 4948 5925 1203
Size: 1573 2172 977 1327
Fragments arranged by size:
2172 1573 1327 977
AgeI A'CCGG_T
Cuts at: 4880 4880
Size: 6049
AhdI GACnn_n'nnGTC
Cuts at: 4778 4778
Size: 6049
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6049
AlwNI CAG_nnn'CTG
Cuts at: 2053 2158 3118 3233 3792 5364 2053
Size: 105 960 115 559 1572 2738
Fragments arranged by size:
2738 1572 960 559 115 105
ApaI G_GGCC'C
Cuts at: 3345 3345
Size: 6049
ApaLI G'TGCA_C
Cuts at: 2102 4106 5262 2102
Size: 2004 1156 2889
Fragments arranged by size:
2889 2004 1156
ApoI r'AATT_y
Cuts at: 1493 2646 2729 2932 3006 3358 3584 3736 5845
Size: 1153 83 203 74 352 226 152 2109
Cuts at: 5845 1493
Size: 1697
Fragments arranged by size:
2109 1697 1153 352 226 203 152 83
74
AseI AT'TA_AT
Cuts at: 160 1032 4603 160
Size: 872 3571 1606
Fragments arranged by size:
3571 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
AvaI C'yCGr_G
Cuts at: 1870 1943 2376 2563 3374 1870
Size: 73 433 187 811 4545
Fragments arranged by size:
4545 811 433 187 73
BamHI G'GATC_C
Cuts at: 3379 3746 3379
Size: 367 5682
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2974 3370 4825 618
Size: 325 217 627 1187 396 1455 1842
Fragments arranged by size:
1842 1455 1187 627 396 325 217
BanII G_rGCy'C
Cuts at: 1391 3345 3368 1391
Size: 1954 23 4072
Fragments arranged by size:
4072 1954 23
BbeI G_GCGC'C
Cuts at: 2978 2978
Size: 6049
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2439 928
Size: 971 540 4538
Fragments arranged by size:
4538 971 540
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4240 4274 1903
Size: 34 50 34 2219 34 3678
Fragments arranged by size:
3678 2219 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3941 5151 3941
Size: 1210 4839
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6049
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4660 136
Size: 107 122 71 631 605 2988 1525
Fragments arranged by size:
2988 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6044 6044
Size: 6049
BlpI GC'TnA_GC
Cuts at: 3466 3466
Size: 6049
Bme1580I G_kGCm'C
Cuts at: 1790 2106 3345 4110 5266 1790
Size: 316 1239 765 1156 2573
Fragments arranged by size:
2573 1239 1156 765 316
BmgBI CAC'GTC
Cuts at: 1318 2854 1318
Size: 1536 4513
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3780 4738 442
Size: 416 53 83 399 24 2363 958 1753
Fragments arranged by size:
2363 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2018 1056
Size: 962 5087
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3043 3095 3338 4709 1239
Size: 1804 52 243 1371 2579
Fragments arranged by size:
2579 1804 1371 243 52
Bpu10I CC'TnA_GC
Cuts at: 3028 3028
Size: 6049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4174 5039 5337 4174
Size: 865 298 4886
Fragments arranged by size:
4886 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4712 882
Size: 617 3213 2219
Fragments arranged by size:
3213 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3840 493
Size: 711 2636 2702
Fragments arranged by size:
2702 2636 711
BsaBI GATnn'nnATC
Cuts at: 2205 6043 2205
Size: 3838 2211
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2975 3856 4238 4873
Size: 53 83 186 936 1442 881 382 635
Cuts at: 4873 275
Size: 1451
Fragments arranged by size:
1451 1442 936 881 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4482 4880 5154 5301 5712 4482
Size: 398 274 147 411 4819
Fragments arranged by size:
4819 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2526 2556 1178
Size: 30 165 30 1123 30 4671
Fragments arranged by size:
4671 1123 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3335 1186
Size: 2149 3900
BseYI C'CCAG_C
Cuts at: 2404 5252 5748 2404
Size: 2848 496 2705
Fragments arranged by size:
2848 2705 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3284 1163
Size: 573 1548 3928
Fragments arranged by size:
3928 1548 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3499 4260 5288 1540
Size: 275 186 1498 761 1028 2301
Fragments arranged by size:
2301 1498 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 4110 4195 5266 2106
Size: 2004 85 1071 2889
Fragments arranged by size:
2889 2004 1071 85
BsmI GAATG_Cn'
Cuts at: 3560 3653 3560
Size: 93 5956
BsmBI CGTCTCn'nnnn_
Cuts at: 2844 2844
Size: 6049
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 3345 3368 4110 4195 5266 1391
Size: 399 316 1239 23 742 85 1071 2174
Fragments arranged by size:
2174 1239 1071 742 399 316 85 23
BspEI T'CCGG_A
Cuts at: 5712 5712
Size: 6049
BspHI T'CATG_A
Cuts at: 3938 5668 3938
Size: 1730 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3404 844
Size: 2560 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3936 1394
Size: 429 2113 3507
Fragments arranged by size:
3507 2113 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2250 4544 4718 65
Size: 1183 1002 2294 174 1396
Fragments arranged by size:
2294 1396 1183 1002 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4693 4880 1700
Size: 110 2883 187 2869
Fragments arranged by size:
2883 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2723 3181 96
Size: 2627 458 2964
Fragments arranged by size:
2964 2627 458
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6049
BssSI C'ACGA_G
Cuts at: 4109 5121 4109
Size: 1012 5037
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6049
BstBI TT'CG_AA
Cuts at: 4853 4853
Size: 6049
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6049
BstXI CCAn_nnnn'nTGG
Cuts at: 2272 2272
Size: 6049
BstYI r'GATC_y
Cuts at: 1972 2070 2744 3095 3379 3746 4131 4148 5600
Size: 98 674 351 284 367 385 17 1452
Cuts at: 5600 5686 5698 6044 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 674 385 367 351 346 284
98 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2037 3288 513
Size: 552 972 1251 3274
Fragments arranged by size:
3274 1251 972 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5310
Fragments arranged by size:
5310 683 56
BtsI GCAGTG_nn'
Cuts at: 3128 3565 4359 4379 3128
Size: 437 794 20 4798
Fragments arranged by size:
4798 794 437 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6014
DraI TTT'AAA
Cuts at: 2835 3353 2835
Size: 518 5531
DraIII CAC_nnn'GTG
Cuts at: 3198 3198
Size: 6049
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5056 809
Size: 507 3740 1802
Fragments arranged by size:
3740 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2479 3496 4385
Size: 54 1425 50 275 18 649 1017 889
Cuts at: 4385 6014 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1017 889 649 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3496 1537
Size: 275 1684 4090
Fragments arranged by size:
4090 1684 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2129 3979 1353
Size: 776 1850 3423
Fragments arranged by size:
3423 1850 776
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3343 4636 5010 5156 387
Size: 1064 138 1754 1293 374 146 1280
Fragments arranged by size:
1754 1293 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2184 2384 2603 3043 3095 3255
Size: 393 478 74 200 219 440 52 160
Cuts at: 3255 3338 4112 4709 5496 5764 1239
Size: 83 774 597 787 268 1524
Fragments arranged by size:
1524 787 774 597 478 440 393 268
219 200 160 83 74 52
EcoO109I rG'GnC_Cy
Cuts at: 3341 3342 3341
Size: 1 6048
EcoRI G'AATT_C
Cuts at: 2729 2932 3006 3358 3736 2729
Size: 203 74 352 378 5042
Fragments arranged by size:
5042 378 352 203 74
EcoRV GAT'ATC
Cuts at: 2507 2507
Size: 6049
FspI TGC'GCA
Cuts at: 4555 4555
Size: 6049
HaeII r_GCGC'y
Cuts at: 1678 2978 3317 5196 1678
Size: 1300 339 1879 2531
Fragments arranged by size:
2531 1879 1300 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2526 2558 2729 2761 4690
Size: 32 135 32 954 32 171 32 1929
Cuts at: 4690 4722 4764 4796 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1929 954 171 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3393 3639 669
Size: 988 281 1455 246 3079
Fragments arranged by size:
3079 1455 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6049
HpaI GTT'AAC
Cuts at: 3639 3639
Size: 6049
KasI G'GCGC_C
Cuts at: 2974 2974
Size: 6049
KpnI G_GTAC'C
Cuts at: 3374 3374
Size: 6049
MfeI C'AATT_G
Cuts at: 3648 3648
Size: 6049
MluI A'CGCG_T
Cuts at: 5925 5925
Size: 6049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2935 3139 5163 5347 1305
Size: 1630 204 2024 184 2007
Fragments arranged by size:
2024 2007 1630 204 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2481 10
Size: 54 1425 343 649 3578
Fragments arranged by size:
3578 1425 649 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2270 3015 3302 4007 4366 4525 518
Size: 648 1104 745 287 705 359 159 2042
Fragments arranged by size:
2042 1104 745 705 648 359 287 159
MspA1I CmG'CkG
Cuts at: 1606 2926 3465 4142 5290 5535 1606
Size: 1320 539 677 1148 245 2120
Fragments arranged by size:
2120 1320 1148 677 539 245
NarI GG'CG_CC
Cuts at: 2975 2975
Size: 6049
NcoI C'CATG_G
Cuts at: 513 1065 2037 513
Size: 552 972 4525
Fragments arranged by size:
4525 972 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6049
NheI G'CTAG_C
Cuts at: 1052 2014 1052
Size: 962 5087
NotI GC'GGCC_GC
Cuts at: 3496 3496
Size: 6049
NspI r_CATG'y
Cuts at: 1132 1229 2780 3407 4952 1132
Size: 97 1551 627 1545 2229
Fragments arranged by size:
2229 1551 1545 627 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6049
PciI A'CATG_T
Cuts at: 2776 4948 2776
Size: 2172 3877
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2053 2081 1108
Size: 902 43 28 5076
Fragments arranged by size:
5076 902 43 28
PmeI GTTT'AAAC
Cuts at: 3353 3353
Size: 6049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 5631 5663 4118
Size: 32 1481 32 4504
Fragments arranged by size:
4504 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6049
PsiI TTA'TAA
Cuts at: 2655 3619 2655
Size: 964 5085
PspOMI G'GGCC_C
Cuts at: 3341 3341
Size: 6049
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2337 2369 2484 2516 2337
Size: 32 115 32 5870
Fragments arranged by size:
5870 115 32 32
PstI C_TGCA'G
Cuts at: 830 2313 3123 3401 830
Size: 1483 810 278 3478
Fragments arranged by size:
3478 1483 810 278
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6049
PvuII CAG'CTG
Cuts at: 2926 2926
Size: 6049
SalI G'TCGA_C
Cuts at: 1936 3391 1936
Size: 1455 4594
SbfI CC_TGCA'GG
Cuts at: 3401 3401
Size: 6049
ScaI AGT'ACT
Cuts at: 4297 6009 4297
Size: 1712 4337
SexAI A'CCwGG_T
Cuts at: 2046 2046
Size: 6049
SfcI C'TryA_G
Cuts at: 826 1045 2175 2309 2937 3119 3397 4532 5213
Size: 219 1130 134 628 182 278 1135 681
Cuts at: 5213 5404 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1130 681 628 278 219 191
182 134
SfoI GGC'GCC
Cuts at: 2976 2976
Size: 6049
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6049
SmaI CCC'GGG
Cuts at: 1872 3376 1872
Size: 1504 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4153 5054 5316 820
Size: 197 926 2210 901 262 1553
Fragments arranged by size:
2210 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3840 493
Size: 3347 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6049
SphI G_CATG'C
Cuts at: 1132 3407 1132
Size: 2275 3774
SspI AAT'ATT
Cuts at: 5 52 2577 3973 5
Size: 47 2525 1396 2081
Fragments arranged by size:
2525 2081 1396 47
StuI AGG'CCT
Cuts at: 1517 2814 1517
Size: 1297 4752
StyI C'CwwG_G
Cuts at: 513 1065 2020 2037 2782 3488 513
Size: 552 955 17 745 706 3074
Fragments arranged by size:
3074 955 745 706 552 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3292 4076 4093 4246 4431 4876 325
Size: 1255 1712 784 17 153 185 445 1498
Fragments arranged by size:
1712 1498 1255 784 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2723 3181 4295
Size: 274 80 33 51 1443 746 458 1114
Cuts at: 4295 6007 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1114 746 458 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6009 6041 6009
Size: 32 6017
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6049
XbaI T'CTAG_A
Cuts at: 3385 3385
Size: 6049
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6049
XmaI C'CCGG_G
Cuts at: 1870 3374 1870
Size: 1504 4545
XmnI GAAnn'nnTTC
Cuts at: 3010 4178 3010
Size: 1168 4881
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3857 276
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI RsrII SacI SacII SanDI SapI SfiI
SrfI SwaI XcmI