Restriction map for
pFN21AB5024
(Circular) (Six-base) MAPSORT of: pFN21AB5024.seq Check: 8446 from: 1 to: 5989
pFN21AB5024 5989 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3274 3274
Size: 5989
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3799 278
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3332 1937
Size: 1395 4594
Acc65I G'GTAC_C
Cuts at: 3310 3310
Size: 5989
AclI AA'CG_TT
Cuts at: 1563 4116 4489 1563
Size: 2553 373 3063
Fragments arranged by size:
3063 2553 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2665 4052 5436 5704 1632
Size: 1033 1387 1384 268 1917
Fragments arranged by size:
1917 1387 1384 1033 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5792
AflIII A'CryG_T
Cuts at: 1203 2085 2472 4888 5865 1203
Size: 882 387 2416 977 1327
Fragments arranged by size:
2416 1327 977 882 387
AgeI A'CCGG_T
Cuts at: 4820 4820
Size: 5989
AhdI GACnn_n'nnGTC
Cuts at: 4718 4718
Size: 5989
AleI CACnn'nnGTG
Cuts at: 1166 3236 3278 1166
Size: 2070 42 3877
Fragments arranged by size:
3877 2070 42
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2553 1184
Size: 1369 4620
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2603 2635 2603
Size: 32 5957
AlwNI CAG_nnn'CTG
Cuts at: 2278 2323 2423 2951 3017 3732 5304 2278
Size: 45 100 528 66 715 1572 2963
Fragments arranged by size:
2963 1572 715 528 100 66 45
ApaI G_GGCC'C
Cuts at: 2031 2743 2031
Size: 712 5277
ApaLI G'TGCA_C
Cuts at: 4046 5202 4046
Size: 1156 4833
ApoI r'AATT_y
Cuts at: 1493 3298 3524 3676 5785 1493
Size: 1805 226 152 2109 1697
Fragments arranged by size:
2109 1805 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4543 160
Size: 872 3511 1606
Fragments arranged by size:
3511 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
AvaI C'yCGr_G
Cuts at: 1870 1943 2348 3314 1870
Size: 73 405 966 4545
Fragments arranged by size:
4545 966 405 73
BamHI G'GATC_C
Cuts at: 3319 3686 3319
Size: 367 5622
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3310 4765 618
Size: 325 217 627 1523 1455 1842
Fragments arranged by size:
1842 1523 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2031 2501 2743 2850 3308 1391
Size: 640 470 242 107 458 4072
Fragments arranged by size:
4072 640 470 458 242 107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5018
BbvCI CC'TCA_GC
Cuts at: 2453 2709 2453
Size: 256 5733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3194 3228 4180 4214 1903
Size: 34 1257 34 952 34 3678
Fragments arranged by size:
3678 1257 952 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3881 5091 3881
Size: 1210 4779
BclI T'GATC_A
Cuts at: 1548 3261 1548
Size: 1713 4276
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2439 2720 2816
Size: 107 122 71 631 605 767 281 96
Cuts at: 2816 3074 4600 136
Size: 258 1526 1525
Fragments arranged by size:
1526 1525 767 631 605 281 258 122
107 96 71
BglII A'GATC_T
Cuts at: 5984 5984
Size: 5989
BlpI GC'TnA_GC
Cuts at: 3018 3406 3018
Size: 388 5601
Bme1580I G_kGCm'C
Cuts at: 1790 2031 2743 4050 5206 1790
Size: 241 712 1307 1156 2573
Fragments arranged by size:
2573 1307 1156 712 241
BmgBI CAC'GTC
Cuts at: 1318 2473 1318
Size: 1155 4834
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2710 3720 4678
Size: 416 53 83 399 24 1293 1010 958
Cuts at: 4678 442
Size: 1753
Fragments arranged by size:
1753 1293 1010 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 4649 1239
Size: 1106 2304 2579
Fragments arranged by size:
2579 2304 1106
Bpu10I CC'TnA_GC
Cuts at: 2453 2709 2453
Size: 256 5733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3017 4114 4979 5277 3017
Size: 1097 865 298 3729
Fragments arranged by size:
3729 1097 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4652 882
Size: 617 3153 2219
Fragments arranged by size:
3153 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2086 3780 493
Size: 711 882 1694 2702
Fragments arranged by size:
2702 1694 882 711
BsaBI GATnn'nnATC
Cuts at: 5983 5983
Size: 5989
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3796 4178 4813 275
Size: 53 83 186 936 2263 382 635 1451
Fragments arranged by size:
2263 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4422 4820 5094 5241 5652 4422
Size: 398 274 147 411 4759
Fragments arranged by size:
4759 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5764
Fragments arranged by size:
5764 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2702 1186
Size: 1516 4473
BseYI C'CCAG_C
Cuts at: 2097 5192 5688 2097
Size: 3095 496 2398
Fragments arranged by size:
3095 2398 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2312 2549 2810 3302 1163
Size: 573 576 237 261 492 3850
Fragments arranged by size:
3850 576 573 492 261 237
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2190 3439 4200 5228 1540
Size: 275 186 189 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 275 189 186
BsiHKAI G_wGCw'C
Cuts at: 2501 2850 4050 4135 5206 2501
Size: 349 1200 85 1071 3284
Fragments arranged by size:
3284 1200 1071 349 85
BsiWI C'GTAC_G
Cuts at: 2834 2834
Size: 5989
BsmI GAATG_Cn'
Cuts at: 3500 3593 3500
Size: 93 5896
BsmBI CGTCTCn'nnnn_
Cuts at: 2177 2177
Size: 5989
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2501 2743 2850 3308 4050 4135
Size: 399 241 470 242 107 458 742 85
Cuts at: 4135 5206 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 470 458 399 242 241
107 85
BspEI T'CCGG_A
Cuts at: 5652 5652
Size: 5989
BspHI T'CATG_A
Cuts at: 2235 3878 5608 2235
Size: 1643 1730 2616
Fragments arranged by size:
2616 1730 1643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2917 3274 3344 844
Size: 2073 357 70 3489
Fragments arranged by size:
3489 2073 357 70
BsrBI CCG'CTC
Cuts at: 1394 1823 3022 3876 1394
Size: 429 1199 854 3507
Fragments arranged by size:
3507 1199 854 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4484 4658 65
Size: 1183 3236 174 1396
Fragments arranged by size:
3236 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2865 4633 4820 1700
Size: 110 1055 1768 187 2869
Fragments arranged by size:
2869 1768 1055 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5989
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5989
BssSI C'ACGA_G
Cuts at: 4049 5061 4049
Size: 1012 4977
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5989
BstBI TT'CG_AA
Cuts at: 4793 4793
Size: 5989
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5989
BstYI r'GATC_y
Cuts at: 1972 2448 2757 2826 2958 3319 3686 4071 4088
Size: 476 309 69 132 361 367 385 17
Cuts at: 4088 5540 5626 5638 5984 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 476 385 367 361 346 309
132 86 69 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2259 2523 2811 513
Size: 552 939 255 264 288 3691
Fragments arranged by size:
3691 939 552 288 264 255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2836 3014 487
Size: 683 56 1610 178 3462
Fragments arranged by size:
3462 1610 683 178 56
BtsI GCAGTG_nn'
Cuts at: 3505 4299 4319 3505
Size: 794 20 5175
Fragments arranged by size:
5175 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5954
DraI TTT'AAA
Cuts at: 3293 3293
Size: 5989
DraIII CAC_nnn'GTG
Cuts at: 2023 2023
Size: 5989
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4996 809
Size: 507 3680 1802
Fragments arranged by size:
3680 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2256 2431
Size: 54 1425 50 275 18 357 69 175
Cuts at: 2431 2553 3201 3436 4325 5954 8
Size: 122 648 235 889 1629 43
Fragments arranged by size:
1629 1425 889 648 357 275 235 175
122 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2187 3436 1537
Size: 275 375 1249 4090
Fragments arranged by size:
4090 1249 375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2763 3919 1353
Size: 1410 1156 3423
Fragments arranged by size:
3423 1410 1156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2993 4576 4950 5096 387
Size: 1064 138 1404 1583 374 146 1280
Fragments arranged by size:
1583 1404 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2499 2848 2499
Size: 349 5640
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2665 4052 4649 5436 5704 1239
Size: 393 713 320 1387 597 787 268 1524
Fragments arranged by size:
1524 1387 787 713 597 393 320 268
EcoO109I rG'GnC_Cy
Cuts at: 2027 2352 2739 2929 3091 2027
Size: 325 387 190 162 4925
Fragments arranged by size:
4925 387 325 190 162
EcoRI G'AATT_C
Cuts at: 3298 3676 3298
Size: 378 5611
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2674 2695 3094 2674
Size: 21 399 5569
Fragments arranged by size:
5569 399 21
FspI TGC'GCA
Cuts at: 4495 4495
Size: 5989
HaeII r_GCGC'y
Cuts at: 1678 2042 5136 1678
Size: 364 3094 2531
Fragments arranged by size:
3094 2531 364
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4630 4662 4704 4736 1373
Size: 32 135 32 3058 32 42 32 2626
Fragments arranged by size:
3058 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2695 3333 3579 669
Size: 988 281 757 638 246 3079
Fragments arranged by size:
3079 988 757 638 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5989
HpaI GTT'AAC
Cuts at: 2695 3579 2695
Size: 884 5105
KpnI G_GTAC'C
Cuts at: 3314 3314
Size: 5989
MfeI C'AATT_G
Cuts at: 2011 3588 2011
Size: 1577 4412
MluI A'CGCG_T
Cuts at: 5865 5865
Size: 5989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 2148 5103 5287 1305
Size: 789 54 2955 184 2007
Fragments arranged by size:
2955 2007 789 184 54
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4167
Fragments arranged by size:
4167 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2549 3236 3278 3947 4306 4465 518
Size: 648 1383 687 42 669 359 159 2042
Fragments arranged by size:
2042 1383 687 669 648 359 159 42
MspA1I CmG'CkG
Cuts at: 1606 2169 2708 3405 4082 5230 5475 1606
Size: 563 539 697 677 1148 245 2120
Fragments arranged by size:
2120 1148 697 677 563 539 245
NaeI GCC'GGC
Cuts at: 2867 2867
Size: 5989
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4498
Fragments arranged by size:
4498 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5989
NgoMIV G'CCGG_C
Cuts at: 2865 2865
Size: 5989
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5989
NotI GC'GGCC_GC
Cuts at: 3436 3436
Size: 5989
NspI r_CATG'y
Cuts at: 1132 1229 3227 3347 4892 1132
Size: 97 1998 120 1545 2229
Fragments arranged by size:
2229 1998 1545 120 97
PasI CC'CwG_GG
Cuts at: 1348 2503 1348
Size: 1155 4834
PciI A'CATG_T
Cuts at: 4888 4888
Size: 5989
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2023 1108
Size: 915 5074
PfoI T'CCnGG_A
Cuts at: 2580 2580
Size: 5989
PmeI GTTT'AAAC
Cuts at: 3293 3293
Size: 5989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 5571 5603 4058
Size: 32 1481 32 4444
Fragments arranged by size:
4444 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2352 2929 2352
Size: 577 5412
PshAI GACnn'nnGTC
Cuts at: 1613 2216 1613
Size: 603 5386
PsiI TTA'TAA
Cuts at: 3559 3559
Size: 5989
PspOMI G'GGCC_C
Cuts at: 2027 2739 2027
Size: 712 5277
PstI C_TGCA'G
Cuts at: 830 2319 2736 3341 830
Size: 1489 417 605 3478
Fragments arranged by size:
3478 1489 605 417
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5989
PvuII CAG'CTG
Cuts at: 2708 2708
Size: 5989
SacI G_AGCT'C
Cuts at: 2501 2850 2501
Size: 349 5640
SalI G'TCGA_C
Cuts at: 1936 3331 1936
Size: 1395 4594
SanDI GG'GwC_CC
Cuts at: 2352 2352
Size: 5989
SbfI CC_TGCA'GG
Cuts at: 3341 3341
Size: 5989
ScaI AGT'ACT
Cuts at: 4237 5949 4237
Size: 1712 4277
SfcI C'TryA_G
Cuts at: 826 1045 2315 2732 2856 3337 4472 5153 5344
Size: 219 1270 417 124 481 1135 681 191
Cuts at: 5344 826
Size: 1471
Fragments arranged by size:
1471 1270 1135 681 481 417 219 191
124
SfiI GGCCn_nnn'nGGCC
Cuts at: 2720 2720
Size: 5989
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5989
SmaI CCC'GGG
Cuts at: 1872 3316 1872
Size: 1444 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3032 4093 4994 5256 820
Size: 197 926 1089 1061 901 262 1553
Fragments arranged by size:
1553 1089 1061 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3780 493
Size: 3287 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5989
SphI G_CATG'C
Cuts at: 1132 3227 3347 1132
Size: 2095 120 3774
Fragments arranged by size:
3774 2095 120
SspI AAT'ATT
Cuts at: 5 52 3913 5
Size: 47 3861 2081
Fragments arranged by size:
3861 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5989
StyI C'CwwG_G
Cuts at: 513 1065 2004 3078 3132 3428 513
Size: 552 939 1074 54 296 3074
Fragments arranged by size:
3074 1074 939 552 296 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4016 4033 4186 4371 4816 325
Size: 1255 2436 17 153 185 445 1498
Fragments arranged by size:
2436 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4235 5947 96
Size: 274 80 33 51 1443 2258 1712 138
Fragments arranged by size:
2258 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2576 2608 2811 2843 5949 5981 2576
Size: 32 203 32 3106 32 2584
Fragments arranged by size:
3106 2584 203 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5989
XbaI T'CTAG_A
Cuts at: 3325 3325
Size: 5989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 3076 2038
Size: 1038 4951
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5989
XmaI C'CCGG_G
Cuts at: 1870 3314 1870
Size: 1444 4545
XmnI GAAnn'nnTTC
Cuts at: 4118 4118
Size: 5989
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3797 276
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BstXI
BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SapI SexAI SfoI SrfI SwaI