Restriction map for
pFN21AB5017
(Circular) (Six-base) MAPSORT of: pFN21AB5017.seq Check: 5900 from: 1 to: 5719
pFN21AB5017 5719 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3529 278
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3062 1937
Size: 1125 4594
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2562 2977 3782 5166 5434 1632
Size: 930 415 805 1384 268 1917
Fragments arranged by size:
1917 1384 930 805 415 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 2865 4618 5595 1203
Size: 1662 1753 977 1327
Fragments arranged by size:
1753 1662 1327 977
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 4448 4448
Size: 5719
AleI CACnn'nnGTG
Cuts at: 1166 2018 1166
Size: 852 4867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2119 2822 1184
Size: 935 703 4081
Fragments arranged by size:
4081 935 703
AlwNI CAG_nnn'CTG
Cuts at: 2776 3462 5034 2776
Size: 686 1572 3461
Fragments arranged by size:
3461 1572 686
ApaI G_GGCC'C
Cuts at: 2517 2569 2517
Size: 52 5667
ApaLI G'TGCA_C
Cuts at: 3776 4932 3776
Size: 1156 4563
ApoI r'AATT_y
Cuts at: 1493 3028 3254 3406 5515 1493
Size: 1535 226 152 2109 1697
Fragments arranged by size:
2109 1697 1535 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 2318 2568 2810 3044 1870
Size: 73 375 250 242 234 4545
Fragments arranged by size:
4545 375 250 242 234 73
BamHI G'GATC_C
Cuts at: 3049 3416 3049
Size: 367 5352
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2129 2384 2741 3040 4495
Size: 325 217 627 342 255 357 299 1455
Cuts at: 4495 618
Size: 1842
Fragments arranged by size:
1842 1455 627 357 342 325 299 255
217
BanII G_rGCy'C
Cuts at: 1391 2246 2436 2517 2569 2723 3038 1391
Size: 855 190 81 52 154 315 4072
Fragments arranged by size:
4072 855 315 190 154 81 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4748
BbvCI CC'TCA_GC
Cuts at: 2207 2207
Size: 5719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3910 3944 1903
Size: 34 1973 34 3678
Fragments arranged by size:
3678 1973 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3611 4821 3611
Size: 1210 4509
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2946 4330 136
Size: 107 122 71 631 605 1274 1384 1525
Fragments arranged by size:
1525 1384 1274 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5714 5714
Size: 5719
BlpI GC'TnA_GC
Cuts at: 3136 3136
Size: 5719
Bme1580I G_kGCm'C
Cuts at: 1790 2132 2517 2557 2569 3780 4936 1790
Size: 342 385 40 12 1211 1156 2573
Fragments arranged by size:
2573 1211 1156 385 342 40 12
BmgBI CAC'GTC
Cuts at: 1318 2676 1318
Size: 1358 4361
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2085 2622 3450
Size: 416 53 83 399 24 668 537 828
Cuts at: 3450 4408 442
Size: 958 1753
Fragments arranged by size:
1753 958 828 668 537 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2323 2582 2749 2914 4379 1239
Size: 1084 259 167 165 1465 2579
Fragments arranged by size:
2579 1465 1084 259 167 165
Bpu10I CC'TnA_GC
Cuts at: 2207 2921 2207
Size: 714 5005
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2062 2201 2959 3844 4709 5007 2062
Size: 139 758 885 865 298 2774
Fragments arranged by size:
2774 885 865 758 298 139
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2259 4382 882
Size: 617 760 2123 2219
Fragments arranged by size:
2219 2123 760 617
BsaAI yAC'GTr
Cuts at: 493 1204 3510 493
Size: 711 2306 2702
Fragments arranged by size:
2702 2306 711
BsaBI GATnn'nnATC
Cuts at: 5713 5713
Size: 5719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2980 3526 3908 4543
Size: 53 83 186 936 1447 546 382 635
Cuts at: 4543 275
Size: 1451
Fragments arranged by size:
1451 1447 936 635 546 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4152 4550 4824 4971 5382 4152
Size: 398 274 147 411 4489
Fragments arranged by size:
4489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5494
Fragments arranged by size:
5494 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 1186
Size: 1009 4710
BseYI C'CCAG_C
Cuts at: 2177 2618 2882 4922 5418 2177
Size: 441 264 2040 496 2478
Fragments arranged by size:
2478 2040 496 441 264
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2115 1163
Size: 573 379 4767
Fragments arranged by size:
4767 573 379
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3169 3930 4958 1540
Size: 275 186 1168 761 1028 2301
Fragments arranged by size:
2301 1168 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2089 2246 2436 3780 3865 4936 2089
Size: 157 190 1344 85 1071 2872
Fragments arranged by size:
2872 1344 1071 190 157 85
BsmI GAATG_Cn'
Cuts at: 3230 3323 3230
Size: 93 5626
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2132 2246 2436 2517 2557 2569
Size: 399 299 43 114 190 81 40 12
Cuts at: 2569 2723 3038 3780 3865 4936 1391
Size: 154 315 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 315 299 190 154
114 85 81 43 40 12
BspEI T'CCGG_A
Cuts at: 5382 5382
Size: 5719
BspHI T'CATG_A
Cuts at: 3608 5338 3608
Size: 1730 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3074 844
Size: 2230 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2237 2271 2293 3606 1394
Size: 429 414 34 22 1313 3507
Fragments arranged by size:
3507 1313 429 414 34 22
BsrDI GCAATG_nn'
Cuts at: 65 1248 4214 4388 65
Size: 1183 2966 174 1396
Fragments arranged by size:
2966 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4363 4550 1700
Size: 110 2553 187 2869
Fragments arranged by size:
2869 2553 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 2018 2429 3779 4791 2018
Size: 411 1350 1012 2946
Fragments arranged by size:
2946 1350 1012 411
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5719
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5719
BstYI r'GATC_y
Cuts at: 1972 3049 3416 3801 3818 5270 5356 5368 5714
Size: 1077 367 385 17 1452 86 12 346
Cuts at: 5714 1972
Size: 1977
Fragments arranged by size:
1977 1452 1077 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2889 2889
Size: 5719
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5167
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4980
Fragments arranged by size:
4980 683 56
BtsI GCAGTG_nn'
Cuts at: 2130 3235 4029 4049 2130
Size: 1105 794 20 3800
Fragments arranged by size:
3800 1105 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4726 809
Size: 507 3410 1802
Fragments arranged by size:
3410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2326 2559 3166
Size: 54 1425 50 275 18 496 233 607
Cuts at: 3166 4055 5684 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 607 496 275 233 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3166 1537
Size: 275 1354 4090
Fragments arranged by size:
4090 1354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2538 2843 3649 1353
Size: 1185 305 806 3423
Fragments arranged by size:
3423 1185 806 305
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4306 4680 4826 387
Size: 1064 138 2717 374 146 1280
Fragments arranged by size:
2717 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2244 2434 2244
Size: 190 5529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2323 2562 2582 2749 2914 2977 3782
Size: 393 691 239 20 167 165 63 805
Cuts at: 3782 4379 5166 5434 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 805 787 691 597 393 268 239
167 165 63 20
EcoO109I rG'GnC_Cy
Cuts at: 2514 2565 2572 2514
Size: 51 7 5661
Fragments arranged by size:
5661 51 7
EcoRI G'AATT_C
Cuts at: 3028 3406 3028
Size: 378 5341
FspI TGC'GCA
Cuts at: 4225 4225
Size: 5719
HaeII r_GCGC'y
Cuts at: 1678 2551 2706 4866 1678
Size: 873 155 2160 2531
Fragments arranged by size:
2531 2160 873 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2885 2917 2991 3023 4360
Size: 32 135 32 1313 32 74 32 1337
Cuts at: 4360 4392 4434 4466 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1337 1313 135 74 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2335 3063 3309 669
Size: 988 281 397 728 246 3079
Fragments arranged by size:
3079 988 728 397 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5719
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4833 5017 1305
Size: 3528 184 2007
Fragments arranged by size:
3528 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3897
Fragments arranged by size:
3897 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2018 3677 4036 4195 518
Size: 648 852 1659 359 159 2042
Fragments arranged by size:
2042 1659 852 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2520 2582 2636 2648 3135 3812 4960 5205
Size: 914 62 54 12 487 677 1148 245
Cuts at: 5205 1606
Size: 2120
Fragments arranged by size:
2120 1148 914 677 487 245 62 54
12
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5167
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NruI TCG'CGA
Cuts at: 2248 2248
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 2869 3077 4622 1132
Size: 97 1640 208 1545 2229
Fragments arranged by size:
2229 1640 1545 208 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5719
PciI A'CATG_T
Cuts at: 2865 4618 2865
Size: 1753 3966
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2776 1108
Size: 1668 4051
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2572 2572
Size: 5719
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PspOMI G'GGCC_C
Cuts at: 2513 2565 2513
Size: 52 5667
PstI C_TGCA'G
Cuts at: 830 3071 830
Size: 2241 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
SacI G_AGCT'C
Cuts at: 2246 2436 2246
Size: 190 5529
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SapI GCTCTTCn'nnn_
Cuts at: 2538 2538
Size: 5719
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 3967 5679 3967
Size: 1712 4007
SfcI C'TryA_G
Cuts at: 826 1045 2080 3067 4202 4883 5074 826
Size: 219 1035 987 1135 681 191 1471
Fragments arranged by size:
1471 1135 1035 987 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 2320 2570 2812 3046 1872
Size: 448 250 242 234 4545
Fragments arranged by size:
4545 448 250 242 234
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2041 2216 2974 3823 4724 4986
Size: 197 926 98 175 758 849 901 262
Cuts at: 4986 820
Size: 1553
Fragments arranged by size:
1553 926 901 849 758 262 197 175
98
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 3077 1132
Size: 1945 3774
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5719
StyI C'CwwG_G
Cuts at: 513 1065 2964 3158 513
Size: 552 1899 194 3074
Fragments arranged by size:
3074 1899 552 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 3746 3763 3916 4101 4546 325
Size: 1255 741 1425 17 153 185 445 1498
Fragments arranged by size:
1498 1425 1255 741 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2460 3965 5677
Size: 274 80 33 51 1443 483 1505 1712
Cuts at: 5677 96
Size: 138
Fragments arranged by size:
1712 1505 1443 483 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2409 2441 5679 5711 2409
Size: 32 3238 32 2417
Fragments arranged by size:
3238 2417 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2659 2773 2659
Size: 114 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5719
XmaI C'CCGG_G
Cuts at: 1870 2318 2568 2810 3044 1870
Size: 448 250 242 234 4545
Fragments arranged by size:
4545 448 250 242 234
XmnI GAAnn'nnTTC
Cuts at: 3848 3848
Size: 5719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3527 276
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI