Restriction map for
pFN21AB5014
(Circular) (Six-base) MAPSORT of: pFN21AB5014.seq Check: 8191 from: 1 to: 5956
pFN21AB5014 5956 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2067 3299 1937
Size: 130 1232 4594
Fragments arranged by size:
4594 1232 130
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4019 5403 5671 1632
Size: 2387 1384 268 1917
Fragments arranged by size:
2387 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3104 820
Size: 197 2087 3672
Fragments arranged by size:
3672 2087 197
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2483 1184
Size: 1299 4657
AlwNI CAG_nnn'CTG
Cuts at: 2086 2275 3699 5271 2086
Size: 189 1424 1572 2771
Fragments arranged by size:
2771 1572 1424 189
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 2147 3265 3491 3643 5752 1493
Size: 654 1118 226 152 2109 1697
Fragments arranged by size:
2109 1697 1118 654 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 2720 2753 2894 3281 1870
Size: 73 777 33 141 387 4545
Fragments arranged by size:
4545 777 387 141 73 33
BamHI G'GATC_C
Cuts at: 2632 2871 3286 3653 2632
Size: 239 415 367 4935
Fragments arranged by size:
4935 415 367 239
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2423 2975 3277 4732 618
Size: 325 217 627 636 552 302 1455 1842
Fragments arranged by size:
1842 1455 636 627 552 325 302 217
BanII G_rGCy'C
Cuts at: 1391 3275 1391
Size: 1884 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2601 2661 3126 928
Size: 971 702 60 465 3758
Fragments arranged by size:
3758 971 702 465 60
BbvCI CC'TCA_GC
Cuts at: 3021 3021
Size: 5956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4147 4181 1903
Size: 34 50 34 2126 34 3678
Fragments arranged by size:
3678 2126 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 2472 1548
Size: 924 5032
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2040 4567 136
Size: 107 122 71 631 605 368 2527 1525
Fragments arranged by size:
2527 1525 631 605 368 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 2867 4017 5173 1790
Size: 1077 1150 1156 2573
Fragments arranged by size:
2573 1156 1150 1077
BmgBI CAC'GTC
Cuts at: 1318 3042 1318
Size: 1724 4232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2668 2719 2770
Size: 416 53 83 399 24 1251 51 51
Cuts at: 2770 2789 2907 3687 4645 442
Size: 19 118 780 958 1753
Fragments arranged by size:
1753 1251 958 780 416 399 118 83
53 51 51 24 19
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4616 1239
Size: 3377 2579
Bpu10I CC'TnA_GC
Cuts at: 3021 3021
Size: 5956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 4946 5244 4081
Size: 865 298 4793
Fragments arranged by size:
4793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4619 882
Size: 617 3120 2219
Fragments arranged by size:
3120 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3763 4145 4780 275
Size: 53 83 186 936 2230 382 635 1451
Fragments arranged by size:
2230 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2650 4389 4787 5061 5208 5619 2650
Size: 1739 398 274 147 411 2987
Fragments arranged by size:
2987 1739 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5731
Fragments arranged by size:
5731 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2198 3038 3107 3125 3152 3155 3158
Size: 841 171 840 69 18 27 3 3
Cuts at: 3158 3203 3212 3233 1186
Size: 45 9 21 3909
Fragments arranged by size:
3909 841 840 171 69 45 27 21
18 9 3 3
BseYI C'CCAG_C
Cuts at: 2944 5159 5655 2944
Size: 2215 496 3245
Fragments arranged by size:
3245 2215 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2303 1163
Size: 573 567 4816
Fragments arranged by size:
4816 573 567
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4017 4102 5173 4017
Size: 85 1071 4800
Fragments arranged by size:
4800 1071 85
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
BsmBI CGTCTCn'nnnn_
Cuts at: 2088 3032 2088
Size: 944 5012
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2867 3275 4017 4102 5173 1391
Size: 399 1077 408 742 85 1071 2174
Fragments arranged by size:
2174 1077 1071 742 408 399 85
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2953 3311 844
Size: 2109 358 3489
Fragments arranged by size:
3489 2109 358
BsrBI CCG'CTC
Cuts at: 1394 1823 2668 3843 1394
Size: 429 845 1175 3507
Fragments arranged by size:
3507 1175 845 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4451 4625 65
Size: 1183 3203 174 1396
Fragments arranged by size:
3203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4600 4787 1700
Size: 110 2790 187 2869
Fragments arranged by size:
2869 2790 187 110
BsrGI T'GTAC_A
Cuts at: 96 2517 96
Size: 2421 3535
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 4016 5028 4016
Size: 1012 4944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstYI r'GATC_y
Cuts at: 1972 2632 2871 3286 3653 4038 4055 5507 5593
Size: 660 239 415 367 385 17 1452 86
Cuts at: 5593 5605 5951 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 660 415 385 367 346 239
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2703 2703
Size: 5956
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5404
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 3472 4266 4286 3472
Size: 794 20 5142
Fragments arranged by size:
5142 794 20
ClaI AT'CG_AT
Cuts at: 1554 2679 1554
Size: 1125 4831
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2238 2534
Size: 54 1425 50 275 18 216 192 296
Cuts at: 2534 3235 3403 4292 5921 8
Size: 701 168 889 1629 43
Fragments arranged by size:
1629 1425 889 701 296 275 216 192
168 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2763 3886 1353
Size: 1410 1123 3423
Fragments arranged by size:
3423 1410 1123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4543 4917 5063 387
Size: 1064 138 2954 374 146 1280
Fragments arranged by size:
2954 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4019 4616 5403 5671 1239
Size: 393 2387 597 787 268 1524
Fragments arranged by size:
2387 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2960 2960
Size: 5956
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 5103 1678
Size: 3425 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2617 2649 2668 2700 4597
Size: 32 135 32 1045 32 19 32 1897
Cuts at: 4597 4629 4671 4703 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1897 1045 135 42 32 32 32
32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4134
Fragments arranged by size:
4134 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3914 4273 4432 518
Size: 648 2748 359 159 2042
Fragments arranged by size:
2748 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2112 3372 4049 5197 5442 1606
Size: 417 89 1260 677 1148 245 2120
Fragments arranged by size:
2120 1260 1148 677 417 245 89
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5404
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 2371 3314 4859 1132
Size: 97 1142 943 1545 2229
Fragments arranged by size:
2229 1545 1142 943 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2275 1108
Size: 1167 4789
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2798 2830 2798
Size: 32 5924
PstI C_TGCA'G
Cuts at: 830 3308 830
Size: 2478 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SfcI C'TryA_G
Cuts at: 826 1045 3304 4439 5120 5311 826
Size: 219 2259 1135 681 191 1471
Fragments arranged by size:
2259 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2040 2040
Size: 5956
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3104 4060 4961 5223 820
Size: 197 926 1161 956 901 262 1553
Fragments arranged by size:
1553 1161 956 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5956
StyI C'CwwG_G
Cuts at: 513 1065 2589 3243 3395 513
Size: 552 1524 654 152 3074
Fragments arranged by size:
3074 1524 654 552 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2632 2892 3983 4000 4153 4338 4783
Size: 1255 1052 260 1091 17 153 185 445
Cuts at: 4783 325
Size: 1498
Fragments arranged by size:
1498 1255 1091 1052 445 260 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2517 2814 4202
Size: 274 80 33 51 1443 540 297 1388
Cuts at: 4202 5914 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1388 540 297 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2768 2800 5916 5948 2768
Size: 32 3116 32 2776
Fragments arranged by size:
3116 2776 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3250 3250
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI
PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SapI SexAI SfoI SrfI
SwaI