Restriction map for
pFN21AB5011
(Circular) (Six-base) MAPSORT of: pFN21AB5011.seq Check: 6257 from: 1 to: 5956
pFN21AB5011 5956 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2917 2917
Size: 5956
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 1937
Size: 1362 4594
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4019 5403 5671 1632
Size: 2387 1384 268 1917
Fragments arranged by size:
2387 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 3253 4855 5832 1203
Size: 2050 1602 977 1327
Fragments arranged by size:
2050 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AlwNI CAG_nnn'CTG
Cuts at: 2185 2368 2779 3205 3699 5271 2185
Size: 183 411 426 494 1572 2870
Fragments arranged by size:
2870 1572 494 426 411 183
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 3265 3491 3643 5752 1493
Size: 1772 226 152 2109 1697
Fragments arranged by size:
2109 1772 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 3281 1870
Size: 73 1338 4545
Fragments arranged by size:
4545 1338 73
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3277 4732 618
Size: 325 217 627 1490 1455 1842
Fragments arranged by size:
1842 1490 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2239 3275 1391
Size: 848 1036 4072
Fragments arranged by size:
4072 1036 848
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2589 2676 3237 928
Size: 971 690 87 561 3647
Fragments arranged by size:
3647 971 690 561 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4147 4181 1903
Size: 34 2210 34 3678
Fragments arranged by size:
3678 2210 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2903 4567 136
Size: 107 122 71 631 605 1231 1664 1525
Fragments arranged by size:
1664 1525 1231 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 2111 2745 3373 2111
Size: 634 628 4694
Fragments arranged by size:
4694 634 628
Bme1580I G_kGCm'C
Cuts at: 1790 4017 5173 1790
Size: 2227 1156 2573
Fragments arranged by size:
2573 2227 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3687 4645 442
Size: 416 53 83 399 24 2270 958 1753
Fragments arranged by size:
2270 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2390 3267 4616 1239
Size: 1151 877 1349 2579
Fragments arranged by size:
2579 1349 1151 877
Bpu10I CC'TnA_GC
Cuts at: 3191 3191
Size: 5956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 2685 4081 4946 5244 2414
Size: 271 1396 865 298 3126
Fragments arranged by size:
3126 1396 865 298 271
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4619 882
Size: 617 3120 2219
Fragments arranged by size:
3120 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3763 4145 4780 275
Size: 53 83 186 936 2230 382 635 1451
Fragments arranged by size:
2230 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2061 3102 4389 4787 5061 5208 5619 2061
Size: 1041 1287 398 274 147 411 2398
Fragments arranged by size:
2398 1287 1041 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3240 3270 1178
Size: 30 165 30 1837 30 3864
Fragments arranged by size:
3864 1837 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5956
BseYI C'CCAG_C
Cuts at: 2531 5159 5655 2531
Size: 2628 496 2832
Fragments arranged by size:
2832 2628 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2789 2792 1163
Size: 573 1053 3 4327
Fragments arranged by size:
4327 1053 573 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2239 4017 4102 5173 2239
Size: 1778 85 1071 3022
Fragments arranged by size:
3022 1778 1071 85
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2239 3275 4017 4102 5173 1391
Size: 399 449 1036 742 85 1071 2174
Fragments arranged by size:
2174 1071 1036 742 449 399 85
BspEI T'CCGG_A
Cuts at: 2061 3102 5619 2061
Size: 1041 2517 2398
Fragments arranged by size:
2517 2398 1041
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2706 2917 3311 844
Size: 1862 211 394 3489
Fragments arranged by size:
3489 1862 394 211
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2892 4451 4625 65
Size: 1183 1644 1559 174 1396
Fragments arranged by size:
1644 1559 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4600 4787 1700
Size: 110 2790 187 2869
Fragments arranged by size:
2869 2790 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 4016 5028 4016
Size: 1012 4944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 2272 2623 2272
Size: 351 5605
BstYI r'GATC_y
Cuts at: 1972 3286 3653 4038 4055 5507 5593 5605 5951
Size: 1314 367 385 17 1452 86 12 346
Cuts at: 5951 1972
Size: 1977
Fragments arranged by size:
1977 1452 1314 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4465
Fragments arranged by size:
4465 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 2214 2780 2801 2949 2973 3472 4266 4286 2214
Size: 566 21 148 24 499 794 20 3884
Fragments arranged by size:
3884 794 566 499 148 24 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2026 2766 2889
Size: 54 1425 50 275 18 196 740 123
Cuts at: 2889 3403 4292 5921 8
Size: 514 889 1629 43
Fragments arranged by size:
1629 1425 889 740 514 275 196 123
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 2680 3027 3886 1353
Size: 678 649 347 859 3423
Fragments arranged by size:
3423 859 678 649 347
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4543 4917 5063 387
Size: 1064 138 2954 374 146 1280
Fragments arranged by size:
2954 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2237 2237
Size: 5956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2390 3267 4019 4616 5403 5671 1239
Size: 393 758 877 752 597 787 268 1524
Fragments arranged by size:
1524 877 787 758 752 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2284 2284
Size: 5956
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FspI TGC'GCA
Cuts at: 2201 4462 2201
Size: 2261 3695
HaeII r_GCGC'y
Cuts at: 1678 2186 5103 1678
Size: 508 2917 2531
Fragments arranged by size:
2917 2531 508
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 3097 3129 4597
Size: 32 135 32 518 32 975 32 1468
Cuts at: 4597 4629 4671 4703 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1468 975 518 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2521 5070 5254 1305
Size: 1216 2549 184 2007
Fragments arranged by size:
2549 2007 1216 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2028 2891 10
Size: 54 1425 343 196 863 3075
Fragments arranged by size:
3075 1425 863 343 196 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2270 2462 2993 3914 4273 4432 518
Size: 648 1104 192 531 921 359 159 2042
Fragments arranged by size:
2042 1104 921 648 531 359 192 159
MspA1I CmG'CkG
Cuts at: 1606 2171 2290 3002 3130 3372 4049 5197 5442
Size: 565 119 712 128 242 677 1148 245
Cuts at: 5442 1606
Size: 2120
Fragments arranged by size:
2120 1148 712 677 565 245 242 128
119
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4465
Fragments arranged by size:
4465 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 2489 3314 4859 1132
Size: 97 1260 825 1545 2229
Fragments arranged by size:
2229 1545 1260 825 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2185 2368 1108
Size: 1077 183 4696
Fragments arranged by size:
4696 1077 183
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2284 2284
Size: 5956
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 2792 2916 3526 2792
Size: 124 610 5222
Fragments arranged by size:
5222 610 124
PstI C_TGCA'G
Cuts at: 830 2640 3308 830
Size: 1810 668 3478
Fragments arranged by size:
3478 1810 668
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
PvuII CAG'CTG
Cuts at: 2171 3002 3130 2171
Size: 831 128 4997
Fragments arranged by size:
4997 831 128
SacI G_AGCT'C
Cuts at: 2239 2239
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 2516 4204 5916 2516
Size: 1688 1712 2556
Fragments arranged by size:
2556 1712 1688
SexAI A'CCwGG_T
Cuts at: 2049 2049
Size: 5956
SfcI C'TryA_G
Cuts at: 826 1045 2636 3304 4439 5120 5311 826
Size: 219 1591 668 1135 681 191 1471
Fragments arranged by size:
1591 1471 1135 681 668 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2393 2700 4060 4961 5223 820
Size: 197 926 450 307 1360 901 262 1553
Fragments arranged by size:
1553 1360 926 901 450 307 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 2758 3880 5
Size: 47 2706 1122 2081
Fragments arranged by size:
2706 2081 1122 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5956
StyI C'CwwG_G
Cuts at: 513 1065 2004 2023 3395 513
Size: 552 939 19 1372 3074
Fragments arranged by size:
3074 1372 939 552 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3983 4000 4153 4338 4783 325
Size: 1255 2403 17 153 185 445 1498
Fragments arranged by size:
2403 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2514 4202 5914
Size: 274 80 33 51 1443 537 1688 1712
Cuts at: 5914 96
Size: 138
Fragments arranged by size:
1712 1688 1443 537 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5916 5948 5916
Size: 32 5924
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2186 2528 2899 2186
Size: 342 371 5243
Fragments arranged by size:
5243 371 342
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI