Restriction map for
pFN21AB5010
(Circular) (Six-base) MAPSORT of: pFN21AB5010.seq Check: 6480 from: 1 to: 6562
pFN21AB5010 6562 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3311 3418 3731 3311
Size: 107 313 6142
Fragments arranged by size:
6142 313 107
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4372 278
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3905 1937
Size: 1968 4594
Acc65I G'GTAC_C
Cuts at: 3883 3883
Size: 6562
AclI AA'CG_TT
Cuts at: 1563 4689 5062 1563
Size: 3126 373 3063
Fragments arranged by size:
3126 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2175 2529 3867 4625 6009 6277 1632
Size: 543 354 1338 758 1384 268 1917
Fragments arranged by size:
1917 1384 1338 758 543 354 268
AfeI AGC'GCT
Cuts at: 2054 3611 2054
Size: 1557 5005
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6365
AflIII A'CryG_T
Cuts at: 1203 5461 6438 1203
Size: 4258 977 1327
Fragments arranged by size:
4258 1327 977
AgeI A'CCGG_T
Cuts at: 5393 5393
Size: 6562
AhdI GACnn_n'nnGTC
Cuts at: 5291 5291
Size: 6562
AleI CACnn'nnGTG
Cuts at: 1166 2481 2985 1166
Size: 1315 504 4743
Fragments arranged by size:
4743 1315 504
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6562
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2406 2438 2406
Size: 32 6530
AlwNI CAG_nnn'CTG
Cuts at: 3286 4305 5877 3286
Size: 1019 1572 3971
Fragments arranged by size:
3971 1572 1019
ApaLI G'TGCA_C
Cuts at: 2390 2990 3074 3158 4619 5775 2390
Size: 600 84 84 1461 1156 3177
Fragments arranged by size:
3177 1461 1156 600 84 84
ApoI r'AATT_y
Cuts at: 1493 3871 4097 4249 6358 1493
Size: 2378 226 152 2109 1697
Fragments arranged by size:
2378 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5116 160
Size: 872 4084 1606
Fragments arranged by size:
4084 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 2294 2348 2827 2881 3163 3887
Size: 73 113 238 54 479 54 282 724
Cuts at: 3887 1870
Size: 4545
Fragments arranged by size:
4545 724 479 282 238 113 73 54
54
AvrII C'CTAG_G
Cuts at: 2246 2246
Size: 6562
BamHI G'GATC_C
Cuts at: 3892 4259 3892
Size: 367 6195
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2290 3883 5338 618
Size: 325 217 627 503 1593 1455 1842
Fragments arranged by size:
1842 1593 1455 627 503 325 217
BanII G_rGCy'C
Cuts at: 1391 2628 3627 3822 3881 1391
Size: 1237 999 195 59 4072
Fragments arranged by size:
4072 1237 999 195 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5591
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4753 4787 1903
Size: 34 2816 34 3678
Fragments arranged by size:
3678 2816 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4454 5664 4454
Size: 1210 5352
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6562
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2493 3341 5173
Size: 107 122 71 631 605 821 848 1832
Cuts at: 5173 136
Size: 1525
Fragments arranged by size:
1832 1525 848 821 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6557 6557
Size: 6562
BlpI GC'TnA_GC
Cuts at: 3979 3979
Size: 6562
Bme1580I G_kGCm'C
Cuts at: 1790 2348 2394 2852 2994 3078 3162 3273 3795
Size: 558 46 458 142 84 84 111 522
Cuts at: 3795 4623 5779 1790
Size: 828 1156 2573
Fragments arranged by size:
2573 1156 828 558 522 458 142 111
84 84 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6562
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3541 3640 4293
Size: 416 53 83 399 24 2124 99 653
Cuts at: 4293 5251 442
Size: 958 1753
Fragments arranged by size:
2124 1753 958 653 416 399 99 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6562
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3595 3595
Size: 6562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3354 3836 5222 1239
Size: 2115 482 1386 2579
Fragments arranged by size:
2579 2115 1386 482
Bpu10I CC'TnA_GC
Cuts at: 2196 2196
Size: 6562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2453 2612 3041 3769 4687 5552 5850 2453
Size: 159 429 728 918 865 298 3165
Fragments arranged by size:
3165 918 865 728 429 298 159
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3566 5225 882
Size: 617 2067 1659 2219
Fragments arranged by size:
2219 2067 1659 617
BsaAI yAC'GTr
Cuts at: 493 1204 2724 4353 493
Size: 711 1520 1629 2702
Fragments arranged by size:
2702 1629 1520 711
BsaBI GATnn'nnATC
Cuts at: 6556 6556
Size: 6562
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3044 4369 4751 5386
Size: 53 83 186 936 1511 1325 382 635
Cuts at: 5386 275
Size: 1451
Fragments arranged by size:
1511 1451 1325 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4995 5393 5667 5814 6225 4995
Size: 398 274 147 411 5332
Fragments arranged by size:
5332 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2210 2240 1178
Size: 30 165 30 807 30 5500
Fragments arranged by size:
5500 807 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3119 3539 1186
Size: 1933 420 4209
Fragments arranged by size:
4209 1933 420
BseYI C'CCAG_C
Cuts at: 2035 3358 3674 5765 6261 2035
Size: 1323 316 2091 496 2336
Fragments arranged by size:
2336 2091 1323 496 316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3261 3625 1163
Size: 573 1525 364 4100
Fragments arranged by size:
4100 1525 573 364
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2359 2863 3004 3008 3861 4012
Size: 275 186 358 504 141 4 853 151
Cuts at: 4012 4773 5801 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 853 761 504 358 275 186
151 141 4
BsiHKAI G_wGCw'C
Cuts at: 2394 2628 2994 3078 3162 3627 4623 4708 5779
Size: 234 366 84 84 465 996 85 1071
Cuts at: 5779 2394
Size: 3177
Fragments arranged by size:
3177 1071 996 465 366 234 85 84
84
BsmI GAATG_Cn'
Cuts at: 3464 3846 4073 4166 3464
Size: 382 227 93 5860
Fragments arranged by size:
5860 382 227 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2348 2394 2628 2852 2994 3078 3162
Size: 399 558 46 234 224 142 84 84
Cuts at: 3162 3273 3627 3795 3822 3881 4623 4708 5779
Size: 111 354 168 27 59 742 85 1071
Cuts at: 5779 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 558 399 354 234 224
168 142 111 85 84 84 59 46
27
BspEI T'CCGG_A
Cuts at: 6225 6225
Size: 6562
BspHI T'CATG_A
Cuts at: 4451 6181 4451
Size: 1730 4832
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3311 3418 3731 3917 844
Size: 2467 107 313 186 3489
Fragments arranged by size:
3489 2467 313 186 107
BsrBI CCG'CTC
Cuts at: 1394 1823 2500 2650 2709 3238 4449 1394
Size: 429 677 150 59 529 1211 3507
Fragments arranged by size:
3507 1211 677 529 429 150 59
BsrDI GCAATG_nn'
Cuts at: 65 1248 3029 5057 5231 65
Size: 1183 1781 2028 174 1396
Fragments arranged by size:
2028 1781 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2140 2562 2660 2962 2982 3003 3856
Size: 110 330 422 98 302 20 21 853
Cuts at: 3856 5206 5393 1700
Size: 1350 187 2869
Fragments arranged by size:
2869 1350 853 422 330 302 187 110
98 21 20
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6562
BssHII G'CGCG_C
Cuts at: 1925 2117 2454 2902 1925
Size: 192 337 448 5585
Fragments arranged by size:
5585 448 337 192
BssSI C'ACGA_G
Cuts at: 2813 3233 3605 4622 5634 2813
Size: 420 372 1017 1012 3741
Fragments arranged by size:
3741 1017 1012 420 372
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3648 794
Size: 2854 3708
BstBI TT'CG_AA
Cuts at: 5366 5366
Size: 6562
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6562
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2483 3470 3814 2011
Size: 472 987 344 4759
Fragments arranged by size:
4759 987 472 344
BstYI r'GATC_y
Cuts at: 1972 2472 3892 4259 4644 4661 6113 6199 6211
Size: 500 1420 367 385 17 1452 86 12
Cuts at: 6211 6557 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1420 500 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3224 3224
Size: 6562
BtgI C'CryG_G
Cuts at: 513 1065 2004 2235 2308 3261 3795 513
Size: 552 939 231 73 953 534 3280
Fragments arranged by size:
3280 953 939 552 534 231 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5823
Fragments arranged by size:
5823 683 56
BtsI GCAGTG_nn'
Cuts at: 2434 2929 3259 4078 4872 4892 2434
Size: 495 330 819 794 20 4104
Fragments arranged by size:
4104 819 794 495 330 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6562
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6527
DraI TTT'AAA
Cuts at: 3866 3866
Size: 6562
DraIII CAC_nnn'GTG
Cuts at: 3423 3648 3790 3423
Size: 225 142 6195
Fragments arranged by size:
6195 225 142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5569 809
Size: 507 4253 1802
Fragments arranged by size:
4253 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 2238 2356
Size: 54 1425 50 275 18 333 75 118
Cuts at: 2356 2485 2860 2973 3005 3082 3414 3858 4009
Size: 129 375 113 32 77 332 444 151
Cuts at: 4009 4898 6527 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 444 375 333 332 275
151 129 118 113 77 75 54 50
43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 2356 2860 3005 3858 4009 1537
Size: 275 544 504 145 853 151 4090
Fragments arranged by size:
4090 853 544 504 275 151 145
EarI CTCTTCn'nnn_
Cuts at: 1353 2151 2690 3843 4492 1353
Size: 798 539 1153 649 3423
Fragments arranged by size:
3423 1153 798 649 539
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2981 5149 5523 5669 387
Size: 1064 138 1392 2168 374 146 1280
Fragments arranged by size:
2168 1392 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2626 3625 2626
Size: 999 5563
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2175 2529 3354 3836 3867 4625 5222
Size: 393 543 354 825 482 31 758 597
Cuts at: 5222 6009 6277 1239
Size: 787 268 1524
Fragments arranged by size:
1524 825 787 758 597 543 482 393
354 268 31
EcoNI CCTnn'n_nnAGG
Cuts at: 3372 3372
Size: 6562
EcoO109I rG'GnC_Cy
Cuts at: 2250 2632 3052 3364 3801 2250
Size: 382 420 312 437 5011
Fragments arranged by size:
5011 437 420 382 312
EcoRI G'AATT_C
Cuts at: 3871 4249 3871
Size: 378 6184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3592 3763 3592
Size: 171 6391
FseI GG_CCGG'CC
Cuts at: 3860 3860
Size: 6562
FspI TGC'GCA
Cuts at: 2303 2433 3683 3767 5068 2303
Size: 130 1250 84 1301 3797
Fragments arranged by size:
3797 1301 1250 130 84
FspAI rTGC'GCAy
Cuts at: 2433 2433
Size: 6562
HaeII r_GCGC'y
Cuts at: 1678 2056 2671 3175 3305 3613 5709 1678
Size: 378 615 504 130 308 2096 2531
Fragments arranged by size:
2531 2096 615 504 378 308 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2210 2242 2875
Size: 32 135 32 443 32 163 32 633
Cuts at: 2875 2907 5203 5235 5277 5309 1373
Size: 32 2296 32 42 32 2626
Fragments arranged by size:
2626 2296 633 443 163 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3906 4152 669
Size: 988 281 1968 246 3079
Fragments arranged by size:
3079 1968 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6562
HpaI GTT'AAC
Cuts at: 4152 4152
Size: 6562
KpnI G_GTAC'C
Cuts at: 3887 3887
Size: 6562
MfeI C'AATT_G
Cuts at: 4161 4161
Size: 6562
MluI A'CGCG_T
Cuts at: 6438 6438
Size: 6562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2898 5676 5860 1305
Size: 1593 2778 184 2007
Fragments arranged by size:
2778 2007 1593 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2487 2975 3084 3416 10
Size: 54 1425 343 655 488 109 332 3156
Fragments arranged by size:
3156 1425 655 488 343 332 109 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2481 2985 3443 4520 4879 5038
Size: 648 843 472 504 458 1077 359 159
Cuts at: 5038 518
Size: 2042
Fragments arranged by size:
2042 1077 843 648 504 472 458 359
159
MspA1I CmG'CkG
Cuts at: 1606 2237 2427 2595 2763 3088 3099 3263 3978
Size: 631 190 168 168 325 11 164 715
Cuts at: 3978 4655 5803 6048 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 715 677 631 325 245 190
168 168 164 11
NaeI GCC'GGC
Cuts at: 2142 2964 3858 2142
Size: 822 894 4846
Fragments arranged by size:
4846 894 822
NcoI C'CATG_G
Cuts at: 513 1065 2004 3795 513
Size: 552 939 1791 3280
Fragments arranged by size:
3280 1791 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6562
NgoMIV G'CCGG_C
Cuts at: 2140 2962 3856 2140
Size: 822 894 4846
Fragments arranged by size:
4846 894 822
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6562
NotI GC'GGCC_GC
Cuts at: 2356 2860 4009 2356
Size: 504 1149 4909
Fragments arranged by size:
4909 1149 504
NspI r_CATG'y
Cuts at: 1132 1229 2869 3195 3455 3920 5465 1132
Size: 97 1640 326 260 465 1545 2229
Fragments arranged by size:
2229 1640 1545 465 326 260 97
PasI CC'CwG_GG
Cuts at: 1348 2109 1348
Size: 761 5801
PciI A'CATG_T
Cuts at: 5461 5461
Size: 6562
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3423 1108
Size: 2315 4247
PfoI T'CCnGG_A
Cuts at: 3477 3477
Size: 6562
PmeI GTTT'AAAC
Cuts at: 3866 3866
Size: 6562
PmlI CAC'GTG
Cuts at: 2724 2724
Size: 6562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4631 4663 6144 6176 4631
Size: 32 1481 32 5017
Fragments arranged by size:
5017 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2632 3052 3364 3801 2632
Size: 420 312 437 5393
Fragments arranged by size:
5393 437 420 312
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6562
PsiI TTA'TAA
Cuts at: 4132 4132
Size: 6562
PspXI vC'TCGA_Gb
Cuts at: 2881 2881
Size: 6562
PstI C_TGCA'G
Cuts at: 830 2023 3914 830
Size: 1193 1891 3478
Fragments arranged by size:
3478 1891 1193
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6562
PvuII CAG'CTG
Cuts at: 2595 2595
Size: 6562
SacI G_AGCT'C
Cuts at: 2628 3627 2628
Size: 999 5563
SacII CC_GC'GG
Cuts at: 2238 3264 2238
Size: 1026 5536
SalI G'TCGA_C
Cuts at: 1936 3904 1936
Size: 1968 4594
SanDI GG'GwC_CC
Cuts at: 3364 3801 3364
Size: 437 6125
SapI GCTCTTCn'nnn_
Cuts at: 2690 2690
Size: 6562
SbfI CC_TGCA'GG
Cuts at: 3914 3914
Size: 6562
ScaI AGT'ACT
Cuts at: 4810 6522 4810
Size: 1712 4850
SfcI C'TryA_G
Cuts at: 826 1045 2019 2590 3910 5045 5726 5917 826
Size: 219 974 571 1320 1135 681 191 1471
Fragments arranged by size:
1471 1320 1135 974 681 571 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
SgrAI Cr'CCGG_yG
Cuts at: 1700 2562 2660 2982 1700
Size: 862 98 322 5280
Fragments arranged by size:
5280 862 322 98
SmaI CCC'GGG
Cuts at: 1872 3889 1872
Size: 2017 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2468 2627 2881 3056 3748 4666
Size: 197 926 525 159 254 175 692 918
Cuts at: 4666 5567 5829 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 918 901 692 525 262 254
197 175 159
SnaBI TAC'GTA
Cuts at: 493 4353 493
Size: 3860 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6562
SphI G_CATG'C
Cuts at: 1132 3920 1132
Size: 2788 3774
SspI AAT'ATT
Cuts at: 5 52 4486 5
Size: 47 4434 2081
Fragments arranged by size:
4434 2081 47
StuI AGG'CCT
Cuts at: 1517 2279 2447 3743 1517
Size: 762 168 1296 4336
Fragments arranged by size:
4336 1296 762 168
StyI C'CwwG_G
Cuts at: 513 1065 2004 2246 2274 3495 3738 3795 4001
Size: 552 939 242 28 1221 243 57 206
Cuts at: 4001 513
Size: 3074
Fragments arranged by size:
3074 1221 939 552 243 242 206 57
28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3659 3797 4589 4606 4759 4944 5389
Size: 1255 2079 138 792 17 153 185 445
Cuts at: 5389 325
Size: 1498
Fragments arranged by size:
2079 1498 1255 792 445 185 153 138
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4808 6520 96
Size: 274 80 33 51 1443 2831 1712 138
Fragments arranged by size:
2831 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6522 6554 6522
Size: 32 6530
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6562
XbaI T'CTAG_A
Cuts at: 3898 3898
Size: 6562
XhoI C'TCGA_G
Cuts at: 1943 2881 1943
Size: 938 5624
XmaI C'CCGG_G
Cuts at: 1870 3887 1870
Size: 2017 4545
XmnI GAAnn'nnTTC
Cuts at: 2699 2840 4691 2699
Size: 141 1851 4570
Fragments arranged by size:
4570 1851 141
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4370 276
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI
BstZ17I EcoRV KasI NarI NruI NsiI PacI PspOMI
PsrI RsrII SexAI SfiI SfoI SrfI SwaI XcmI