Restriction map for
pFN21AB5005
(Circular) (Six-base) MAPSORT of: pFN21AB5005.seq Check: 3583 from: 1 to: 5959
pFN21AB5005 5959 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2143 2894 4022 5406 5674 1632
Size: 511 751 1128 1384 268 1917
Fragments arranged by size:
1917 1384 1128 751 511 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2528 1184
Size: 1344 4615
AlwNI CAG_nnn'CTG
Cuts at: 2093 3702 5274 2093
Size: 1609 1572 2778
Fragments arranged by size:
2778 1609 1572
ApaLI G'TGCA_C
Cuts at: 2324 4016 5172 2324
Size: 1692 1156 3111
Fragments arranged by size:
3111 1692 1156
ApoI r'AATT_y
Cuts at: 1493 2642 3268 3494 3646 5755 1493
Size: 1149 626 226 152 2109 1697
Fragments arranged by size:
2109 1697 1149 626 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2561 2837 3284 1870
Size: 73 618 276 447 4545
Fragments arranged by size:
4545 618 447 276 73
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2594 3216 3280 4735 618
Size: 325 217 627 807 622 64 1455 1842
Fragments arranged by size:
1842 1455 807 627 622 325 217 64
BanII G_rGCy'C
Cuts at: 1391 2117 2244 3278 1391
Size: 726 127 1034 4072
Fragments arranged by size:
4072 1034 726 127
BbeI G_GCGC'C
Cuts at: 2598 3220 2598
Size: 622 5337
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4150 4184 1903
Size: 34 50 34 2129 34 3678
Fragments arranged by size:
3678 2129 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2153 4570 136
Size: 107 122 71 631 605 481 2417 1525
Fragments arranged by size:
2417 1525 631 605 481 122 107 71
BglII A'GATC_T
Cuts at: 2877 5954 2877
Size: 3077 2882
BlpI GC'TnA_GC
Cuts at: 2294 3376 2294
Size: 1082 4877
Bme1580I G_kGCm'C
Cuts at: 1790 2328 4020 5176 1790
Size: 538 1692 1156 2573
Fragments arranged by size:
2573 1692 1156 538
BmgBI CAC'GTC
Cuts at: 1318 2578 1318
Size: 1260 4699
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2641 3690 4648
Size: 416 53 83 399 24 1224 1049 958
Cuts at: 4648 442
Size: 1753
Fragments arranged by size:
1753 1224 1049 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2782 4619 1239
Size: 824 719 1837 2579
Fragments arranged by size:
2579 1837 824 719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4084 4949 5247 4084
Size: 865 298 4796
Fragments arranged by size:
4796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4622 882
Size: 617 3123 2219
Fragments arranged by size:
3123 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2827 3750 493
Size: 711 1623 923 2702
Fragments arranged by size:
2702 1623 923 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2273 2595 3217 3766
Size: 53 83 186 936 740 322 622 549
Cuts at: 3766 4148 4783 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 740 635 622 549 382 322
186 83 53
BsaWI w'CCGG_w
Cuts at: 2718 4392 4790 5064 5211 5622 2718
Size: 1674 398 274 147 411 3055
Fragments arranged by size:
3055 1674 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2780 2810 1178
Size: 30 165 30 1377 30 4327
Fragments arranged by size:
4327 1377 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 3140 1186
Size: 1147 807 4005
Fragments arranged by size:
4005 1147 807
BseYI C'CCAG_C
Cuts at: 2236 2251 2392 2414 2969 2974 5162 5658 2236
Size: 15 141 22 555 5 2188 496 2537
Fragments arranged by size:
2537 2188 555 496 141 22 15 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5386
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2328 2478 4020 4105 5176 2328
Size: 150 1542 85 1071 3111
Fragments arranged by size:
3111 1542 1071 150 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
BsmBI CGTCTCn'nnnn_
Cuts at: 3243 3243
Size: 5959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2244 2328 2478 3278 4020 4105
Size: 399 327 127 84 150 800 742 85
Cuts at: 4105 5176 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 800 742 399 327 150 127
85 84
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 2706 3848 5578 2706
Size: 1142 1730 3087
Fragments arranged by size:
3087 1730 1142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2083 2961 3314 844
Size: 1239 878 353 3489
Fragments arranged by size:
3489 1239 878 353
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4454 4628 65
Size: 1183 3206 174 1396
Fragments arranged by size:
3206 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2147 2330 4603 4790 1700
Size: 110 337 183 2273 187 2869
Fragments arranged by size:
2869 2273 337 187 183 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 2372 2508 1925
Size: 447 136 5376
Fragments arranged by size:
5376 447 136
BssSI C'ACGA_G
Cuts at: 2243 4019 5031 2243
Size: 1776 1012 3171
Fragments arranged by size:
3171 1776 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 2566 2941 1184
Size: 1382 375 4202
Fragments arranged by size:
4202 1382 375
BstXI CCAn_nnnn'nTGG
Cuts at: 2578 2932 2578
Size: 354 5605
BstYI r'GATC_y
Cuts at: 1972 2877 3289 3656 4041 4058 5510 5596 5608
Size: 905 412 367 385 17 1452 86 12
Cuts at: 5608 5954 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 905 412 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3231 3231
Size: 5959
BtgI C'CryG_G
Cuts at: 513 1065 2175 2217 2268 2367 2668 2697 513
Size: 552 1110 42 51 99 301 29 3775
Fragments arranged by size:
3775 1110 552 301 99 51 42 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 2097 2199 2295 3475 4269 4289 2097
Size: 102 96 1180 794 20 3767
Fragments arranged by size:
3767 1180 794 102 96 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3055 3090 529
Size: 35 2491 35 3398
Fragments arranged by size:
3398 2491 35 35
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2272 4966 809
Size: 507 956 2694 1802
Fragments arranged by size:
2694 1802 956 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2124 2178
Size: 54 1425 50 275 18 225 69 54
Cuts at: 2178 3048 3406 4295 5924 8
Size: 870 358 889 1629 43
Fragments arranged by size:
1629 1425 889 870 358 275 225 69
54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3889 1353
Size: 2536 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2143 2782 2894 4022 4619 5406
Size: 393 431 80 639 112 1128 597 787
Cuts at: 5406 5674 1239
Size: 268 1524
Fragments arranged by size:
1524 1128 787 639 597 431 393 268
112 80
EcoNI CCTnn'n_nnAGG
Cuts at: 2042 2984 2042
Size: 942 5017
EcoO109I rG'GnC_Cy
Cuts at: 3037 3037
Size: 5959
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2506 3028 2506
Size: 522 5437
FspI TGC'GCA
Cuts at: 2447 3134 4465 2447
Size: 687 1331 3941
Fragments arranged by size:
3941 1331 687
HaeII r_GCGC'y
Cuts at: 1678 2031 2148 2301 2598 2845 3220 5106 1678
Size: 353 117 153 297 247 375 1886 2531
Fragments arranged by size:
2531 1886 375 353 297 247 153 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4600 4632 4674 4706 1373
Size: 32 135 32 3028 32 42 32 2626
Fragments arranged by size:
3028 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2608 3303 3549 669
Size: 988 281 670 695 246 3079
Fragments arranged by size:
3079 988 695 670 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KasI G'GCGC_C
Cuts at: 2594 3216 2594
Size: 622 5337
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3060 5073 5257 1305
Size: 1755 2013 184 2007
Fragments arranged by size:
2013 2007 1755 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2180 3050 10
Size: 54 1425 343 348 870 2919
Fragments arranged by size:
2919 1425 870 348 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 2634 2711 2813 3917 4276 4435
Size: 648 1041 427 77 102 1104 359 159
Cuts at: 4435 518
Size: 2042
Fragments arranged by size:
2042 1104 1041 648 427 359 159 102
77
MspA1I CmG'CkG
Cuts at: 1606 2308 2872 3375 4052 5200 5445 1606
Size: 702 564 503 677 1148 245 2120
Fragments arranged by size:
2120 1148 702 677 564 503 245
NaeI GCC'GGC
Cuts at: 2149 2332 2149
Size: 183 5776
NarI GG'CG_CC
Cuts at: 2595 3217 2595
Size: 622 5337
NcoI C'CATG_G
Cuts at: 513 1065 2175 513
Size: 552 1110 4297
Fragments arranged by size:
4297 1110 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NgoMIV G'CCGG_C
Cuts at: 2147 2330 2147
Size: 183 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3025 3317 4862 1132
Size: 97 1796 292 1545 2229
Fragments arranged by size:
2229 1796 1545 292 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2176 2194 2656 2827 1108
Size: 1068 18 462 171 4240
Fragments arranged by size:
4240 1068 462 171 18
PfoI T'CCnGG_A
Cuts at: 2040 2040
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PmlI CAC'GTG
Cuts at: 2827 2827
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3037 3037
Size: 5959
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2080 2517 2958 3311 830
Size: 1250 437 441 353 3478
Fragments arranged by size:
3478 1250 441 437 353
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
PvuII CAG'CTG
Cuts at: 2308 2872 2308
Size: 564 5395
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2076 2513 2954 3307 4442 5123 5314
Size: 219 1031 437 441 353 1135 681 191
Cuts at: 5314 826
Size: 1471
Fragments arranged by size:
1471 1135 1031 681 441 437 353 219
191
SfoI GGC'GCC
Cuts at: 2596 3218 2596
Size: 622 5337
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2837 4063 4964 5226 820
Size: 197 926 894 1226 901 262 1553
Fragments arranged by size:
1553 1226 926 901 894 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 2727 1517
Size: 1210 4749
StyI C'CwwG_G
Cuts at: 513 1065 2118 2175 2181 2283 2571 2925 3398
Size: 552 1053 57 6 102 288 354 473
Cuts at: 3398 513
Size: 3074
Fragments arranged by size:
3074 1053 552 473 354 288 102 57
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2676 2690 3203 3986 4003 4156 4341
Size: 1255 1096 14 513 783 17 153 185
Cuts at: 4341 4786 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1096 783 513 445 185 153
17 14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4205 5917 96
Size: 274 80 33 51 1443 2228 1712 138
Fragments arranged by size:
2228 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2780 2812 5919 5951 2780
Size: 32 3107 32 2788
Fragments arranged by size:
3107 2788 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2686 1316
Size: 1370 4589
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2932 2932
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 2837 1943
Size: 894 5065
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoRV
FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI