Restriction map for
pFN21AB4995
(Circular) (Six-base) MAPSORT of: pFN21AB4995.seq Check: 2605 from: 1 to: 6727
pFN21AB4995 6727 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2644 2644
Size: 6727
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4537 278
Size: 53 83 186 936 3001 2468
Fragments arranged by size:
3001 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2055 4070 1937
Size: 118 2015 4594
Fragments arranged by size:
4594 2015 118
Acc65I G'GTAC_C
Cuts at: 4048 4048
Size: 6727
AclI AA'CG_TT
Cuts at: 1563 4854 5227 1563
Size: 3291 373 3063
Fragments arranged by size:
3291 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4790 6174 6442 1632
Size: 3158 1384 268 1917
Fragments arranged by size:
3158 1917 1384 268
AfeI AGC'GCT
Cuts at: 2348 2348
Size: 6727
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6530
AflIII A'CryG_T
Cuts at: 1203 4024 5626 6603 1203
Size: 2821 1602 977 1327
Fragments arranged by size:
2821 1602 1327 977
AgeI A'CCGG_T
Cuts at: 3357 5558 3357
Size: 2201 4526
AhdI GACnn_n'nnGTC
Cuts at: 5456 5456
Size: 6727
AleI CACnn'nnGTG
Cuts at: 1166 3333 1166
Size: 2167 4560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6727
AlwNI CAG_nnn'CTG
Cuts at: 2125 2401 3032 3495 3704 4470 6042 2125
Size: 276 631 463 209 766 1572 2810
Fragments arranged by size:
2810 1572 766 631 463 276 209
ApaI G_GGCC'C
Cuts at: 3399 3399
Size: 6727
ApaLI G'TGCA_C
Cuts at: 2153 4784 5940 2153
Size: 2631 1156 2940
Fragments arranged by size:
2940 2631 1156
ApoI r'AATT_y
Cuts at: 1493 4036 4262 4414 6523 1493
Size: 2543 226 152 2109 1697
Fragments arranged by size:
2543 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5281 160
Size: 872 4249 1606
Fragments arranged by size:
4249 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6727
AvaI C'yCGr_G
Cuts at: 1870 1943 2791 2962 3103 3163 3345 4052 1870
Size: 73 848 171 141 60 182 707 4545
Fragments arranged by size:
4545 848 707 182 171 141 73 60
BamHI G'GATC_C
Cuts at: 4057 4424 4057
Size: 367 6360
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3465 4048 5503 618
Size: 325 217 627 1678 583 1455 1842
Fragments arranged by size:
1842 1678 1455 627 583 325 217
BanII G_rGCy'C
Cuts at: 1391 2192 3344 3399 3599 4046 1391
Size: 801 1152 55 200 447 4072
Fragments arranged by size:
4072 1152 801 447 200 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3846 928
Size: 971 1947 3809
Fragments arranged by size:
3809 1947 971
BbvCI CC'TCA_GC
Cuts at: 2840 3503 2840
Size: 663 6064
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2000 2034 2035 2069 2234 2268 4918
Size: 34 63 34 1 34 165 34 2650
Cuts at: 4918 4952 1903
Size: 34 3678
Fragments arranged by size:
3678 2650 165 63 34 34 34 34
34 1
BciVI GTATCCnnnnn_n'
Cuts at: 4619 5829 4619
Size: 1210 5517
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6727
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3379 5338 136
Size: 107 122 71 631 605 1707 1959 1525
Fragments arranged by size:
1959 1707 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2409 2662 6722 2409
Size: 253 4060 2414
Fragments arranged by size:
4060 2414 253
BlpI GC'TnA_GC
Cuts at: 4144 4144
Size: 6727
Bme1580I G_kGCm'C
Cuts at: 1790 2157 2257 3399 4788 5944 1790
Size: 367 100 1142 1389 1156 2573
Fragments arranged by size:
2573 1389 1156 1142 367 100
BmgBI CAC'GTC
Cuts at: 1318 2620 2632 1318
Size: 1302 12 5413
Fragments arranged by size:
5413 1302 12
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2113 4458 5416
Size: 416 53 83 399 24 696 2345 958
Cuts at: 5416 442
Size: 1753
Fragments arranged by size:
2345 1753 958 696 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6727
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2944 2956 2602
Size: 342 12 6373
Fragments arranged by size:
6373 342 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 3157 3169 3181 5387 1239
Size: 1184 734 12 12 2206 2579
Fragments arranged by size:
2579 2206 1184 734 12 12
Bpu10I CC'TnA_GC
Cuts at: 2840 3503 2840
Size: 663 6064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2789 3976 4852 5717 6015 2789
Size: 1187 876 865 298 3501
Fragments arranged by size:
3501 1187 876 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2437 5390 882
Size: 617 938 2953 2219
Fragments arranged by size:
2953 2219 938 617
BsaAI yAC'GTr
Cuts at: 493 1204 4518 493
Size: 711 3314 2702
Fragments arranged by size:
3314 2702 711
BsaBI GATnn'nnATC
Cuts at: 6721 6721
Size: 6727
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2797 2809 3722 4534
Size: 53 83 186 936 1264 12 913 812
Cuts at: 4534 4916 5551 275
Size: 382 635 1451
Fragments arranged by size:
1451 1264 936 913 812 635 382 186
83 53 12
BsaWI w'CCGG_w
Cuts at: 2721 3357 5160 5558 5832 5979 6390 2721
Size: 636 1803 398 274 147 411 3058
Fragments arranged by size:
3058 1803 636 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2546 2576 3236 3266 3347
Size: 30 165 30 1143 30 660 30 81
Cuts at: 3347 3377 1178
Size: 30 4528
Fragments arranged by size:
4528 1143 660 165 81 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 2567 2570 2573 2588 2591 2750 2786
Size: 1219 162 3 3 15 3 159 36
Cuts at: 2786 2789 2987 3029 3124 3127 3130 3133 3136
Size: 3 198 42 95 3 3 3 3
Cuts at: 3136 3139 3142 1186
Size: 3 3 4771
Fragments arranged by size:
4771 1219 198 162 159 95 42 36
15 3 3 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2113 2447 2864 3084 3551 3557 5930 6426 2113
Size: 334 417 220 467 6 2373 496 2414
Fragments arranged by size:
2414 2373 496 467 417 334 220 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2384 1163
Size: 573 648 5506
Fragments arranged by size:
5506 648 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2571 3241 3454 3639 4177 4938
Size: 275 186 570 670 213 185 538 761
Cuts at: 4938 5966 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 670 570 538 275 213
186 185
BsiHKAI G_wGCw'C
Cuts at: 2157 4788 4873 5944 2157
Size: 2631 85 1071 2940
Fragments arranged by size:
2940 2631 1071 85
BsmI GAATG_Cn'
Cuts at: 4238 4331 4238
Size: 93 6634
BsmBI CGTCTCn'nnnn_
Cuts at: 2074 3342 2074
Size: 1268 5459
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2192 2257 3344 3399 3599 4046
Size: 399 367 35 65 1087 55 200 447
Cuts at: 4046 4788 4873 5944 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1087 1071 742 447 399 367 200
85 65 55 35
BspEI T'CCGG_A
Cuts at: 2721 6390 2721
Size: 3669 3058
BspHI T'CATG_A
Cuts at: 4616 6346 4616
Size: 1730 4997
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2119 2644 3688 4082 844
Size: 1275 525 1044 394 3489
Fragments arranged by size:
3489 1275 1044 525 394
BsrBI CCG'CTC
Cuts at: 1394 1823 2032 2077 2802 2940 2946 2952 3132
Size: 429 209 45 725 138 6 6 180
Cuts at: 3132 3183 3207 3252 3264 3276 3282 3288 3369
Size: 51 24 45 12 12 6 6 81
Cuts at: 3369 3420 3826 3835 3883 3942 4614 1394
Size: 51 406 9 48 59 672 3507
Fragments arranged by size:
3507 725 672 429 406 209 180 138
81 59 51 51 48 45 45 24
12 12 9 6 6 6 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 5222 5396 65
Size: 1183 3974 174 1396
Fragments arranged by size:
3974 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2378 3321 3357 3634 3930 5371 5558
Size: 110 568 943 36 277 296 1441 187
Cuts at: 5558 1700
Size: 2869
Fragments arranged by size:
2869 1441 943 568 296 277 187 110
36
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6727
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6727
BssSI C'ACGA_G
Cuts at: 2012 4787 5799 2012
Size: 2775 1012 2940
Fragments arranged by size:
2940 2775 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3032 794
Size: 2238 4489
BstBI TT'CG_AA
Cuts at: 5531 5531
Size: 6727
BstEII G'GTnAC_C
Cuts at: 1184 2910 1184
Size: 1726 5001
BstYI r'GATC_y
Cuts at: 1972 2409 2662 3867 4057 4424 4809 4826 6278
Size: 437 253 1205 190 367 385 17 1452
Cuts at: 6278 6364 6376 6722 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1205 437 385 367 346 253
190 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3461 3726 3461
Size: 265 6462
BtgI C'CryG_G
Cuts at: 513 1065 3214 3316 3855 513
Size: 552 2149 102 539 3385
Fragments arranged by size:
3385 2149 552 539 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3024 487
Size: 683 56 1798 4190
Fragments arranged by size:
4190 1798 683 56
BtsI GCAGTG_nn'
Cuts at: 2402 4243 5037 5057 2402
Size: 1841 794 20 4072
Fragments arranged by size:
4072 1841 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6727
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6692
DraI TTT'AAA
Cuts at: 4031 4031
Size: 6727
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5734 809
Size: 507 4418 1802
Fragments arranged by size:
4418 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 2178 2520
Size: 54 1425 50 275 18 307 41 342
Cuts at: 2520 2568 2694 3238 3319 3451 3636 4174 5063
Size: 48 126 544 81 132 185 538 889
Cuts at: 5063 6692 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 544 538 342 307 275
185 132 126 81 54 50 48 43
41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2568 3238 3451 3636 4174 1537
Size: 275 756 670 213 185 538 4090
Fragments arranged by size:
4090 756 670 538 275 213 185
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 3160 3903 3982 4657 1353
Size: 707 1100 743 79 675 3423
Fragments arranged by size:
3423 1100 743 707 675 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2087 3794 5314 5688 5834 387
Size: 1064 138 498 1707 1520 374 146 1280
Fragments arranged by size:
1707 1520 1280 1064 498 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 3157 3169 3181 4790 5387 6174
Size: 393 791 734 12 12 1609 597 787
Cuts at: 6174 6442 1239
Size: 268 1524
Fragments arranged by size:
1609 1524 791 787 734 597 393 268
12 12
EcoO109I rG'GnC_Cy
Cuts at: 2110 3168 3396 3457 3996 2110
Size: 1058 228 61 539 4841
Fragments arranged by size:
4841 1058 539 228 61
EcoRI G'AATT_C
Cuts at: 4036 4414 4036
Size: 378 6349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3863 3863
Size: 6727
FseI GG_CCGG'CC
Cuts at: 3638 3638
Size: 6727
FspI TGC'GCA
Cuts at: 5233 5233
Size: 6727
HaeII r_GCGC'y
Cuts at: 1678 2350 3123 3298 3761 3991 5874 1678
Size: 672 773 175 463 230 1883 2531
Fragments arranged by size:
2531 1883 773 672 463 230 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2232 2264 2546 2578 5368
Size: 32 135 32 660 32 282 32 2790
Cuts at: 5368 5400 5442 5474 1373
Size: 32 42 32 2626
Fragments arranged by size:
2790 2626 660 282 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2056 4071 4317 669
Size: 988 281 118 2015 246 3079
Fragments arranged by size:
3079 2015 988 281 246 118
HindIII A'AGCT_T
Cuts at: 748 2036 748
Size: 1288 5439
HpaI GTT'AAC
Cuts at: 4317 4317
Size: 6727
KpnI G_GTAC'C
Cuts at: 4052 4052
Size: 6727
MfeI C'AATT_G
Cuts at: 4326 4326
Size: 6727
MluI A'CGCG_T
Cuts at: 6603 6603
Size: 6727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 5841 6025 1305
Size: 996 3540 184 2007
Fragments arranged by size:
3540 2007 996 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4905
Fragments arranged by size:
4905 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2618 3333 4685 5044 5203 518
Size: 648 1452 715 1352 359 159 2042
Fragments arranged by size:
2042 1452 1352 715 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2183 2567 2815 3032 3495 3686 4143 4820
Size: 577 384 248 217 463 191 457 677
Cuts at: 4820 5968 6213 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 577 463 457 384 248
245 217 191
NaeI GCC'GGC
Cuts at: 2380 3323 3636 3932 2380
Size: 943 313 296 5175
Fragments arranged by size:
5175 943 313 296
NcoI C'CATG_G
Cuts at: 513 1065 3855 513
Size: 552 2790 3385
Fragments arranged by size:
3385 2790 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6727
NgoMIV G'CCGG_C
Cuts at: 2378 3321 3634 3930 2378
Size: 943 313 296 5175
Fragments arranged by size:
5175 943 313 296
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6727
NotI GC'GGCC_GC
Cuts at: 4174 4174
Size: 6727
NspI r_CATG'y
Cuts at: 1132 1229 2759 4028 4085 5630 1132
Size: 97 1530 1269 57 1545 2229
Fragments arranged by size:
2229 1545 1530 1269 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6727
PciI A'CATG_T
Cuts at: 4024 5626 4024
Size: 1602 5125
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6727
PmeI GTTT'AAAC
Cuts at: 4031 4031
Size: 6727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4796 4828 6309 6341 4796
Size: 32 1481 32 5182
Fragments arranged by size:
5182 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2110 3168 3996 2110
Size: 1058 828 4841
Fragments arranged by size:
4841 1058 828
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6727
PsiI TTA'TAA
Cuts at: 4297 4297
Size: 6727
PspOMI G'GGCC_C
Cuts at: 3395 3395
Size: 6727
PspXI vC'TCGA_Gb
Cuts at: 3163 3163
Size: 6727
PstI C_TGCA'G
Cuts at: 830 2130 2170 4079 830
Size: 1300 40 1909 3478
Fragments arranged by size:
3478 1909 1300 40
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6727
PvuII CAG'CTG
Cuts at: 3495 3495
Size: 6727
SalI G'TCGA_C
Cuts at: 1936 2054 4069 1936
Size: 118 2015 4594
Fragments arranged by size:
4594 2015 118
SapI GCTCTTCn'nnn_
Cuts at: 3982 3982
Size: 6727
SbfI CC_TGCA'GG
Cuts at: 2130 4079 2130
Size: 1949 4778
ScaI AGT'ACT
Cuts at: 4975 6687 4975
Size: 1712 5015
SfcI C'TryA_G
Cuts at: 826 1045 2126 2166 2350 3439 4075 5210 5891
Size: 219 1081 40 184 1089 636 1135 681
Cuts at: 5891 6082 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1089 1081 681 636 219 191
184 40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6727
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6727
SmaI CCC'GGG
Cuts at: 1872 2964 3105 4054 1872
Size: 1092 141 949 4545
Fragments arranged by size:
4545 1092 949 141
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2768 3163 3991 4831 5732 5994
Size: 197 926 825 395 828 840 901 262
Cuts at: 5994 820
Size: 1553
Fragments arranged by size:
1553 926 901 840 828 825 395 262
197
SnaBI TAC'GTA
Cuts at: 493 4518 493
Size: 4025 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6727
SphI G_CATG'C
Cuts at: 1132 2759 4085 1132
Size: 1627 1326 3774
Fragments arranged by size:
3774 1627 1326
SspI AAT'ATT
Cuts at: 5 52 4651 5
Size: 47 4599 2081
Fragments arranged by size:
4599 2081 47
StuI AGG'CCT
Cuts at: 1517 2358 2436 1517
Size: 841 78 5808
Fragments arranged by size:
5808 841 78
StyI C'CwwG_G
Cuts at: 513 1065 2760 3855 4166 513
Size: 552 1695 1095 311 3074
Fragments arranged by size:
3074 1695 1095 552 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2253 2878 3689 4754 4771 4924 5109
Size: 1255 673 625 811 1065 17 153 185
Cuts at: 5109 5554 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1065 811 673 625 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2787 3924 4973
Size: 274 80 33 51 1443 810 1137 1049
Cuts at: 4973 6685 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1137 1049 810 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3236 3268 3347 3379 6687 6719 3236
Size: 32 79 32 3308 32 3244
Fragments arranged by size:
3308 3244 79 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2708 1316
Size: 1392 5335
XbaI T'CTAG_A
Cuts at: 4063 4063
Size: 6727
XhoI C'TCGA_G
Cuts at: 1943 3163 1943
Size: 1220 5507
XmaI C'CCGG_G
Cuts at: 1870 2962 3103 4052 1870
Size: 1092 141 949 4545
Fragments arranged by size:
4545 1092 949 141
XmnI GAAnn'nnTTC
Cuts at: 4856 4856
Size: 6727
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4535 276
Size: 53 83 186 936 3001 2468
Fragments arranged by size:
3001 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI
BstZ17I DraIII EcoICRI EcoNI EcoRV FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI