Restriction map for
pFN21AB4992
(Circular) (Six-base) MAPSORT of: pFN21AB4992.seq Check: 3321 from: 1 to: 5548
pFN21AB4992 5548 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2489 3358 278
Size: 53 83 186 936 953 869 2468
Fragments arranged by size:
2468 953 936 869 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2891 1937
Size: 954 4594
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 3675 4048 1563
Size: 2112 373 3063
Fragments arranged by size:
3063 2112 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2668 3611 4995 5263 1632
Size: 1036 943 1384 268 1917
Fragments arranged by size:
1917 1384 1036 943 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 4447 5424 1203
Size: 3244 977 1327
Fragments arranged by size:
3244 1327 977
AgeI A'CCGG_T
Cuts at: 4379 4379
Size: 5548
AhdI GACnn_n'nnGTC
Cuts at: 4277 4277
Size: 5548
AleI CACnn'nnGTG
Cuts at: 1166 2030 1166
Size: 864 4684
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2152 1184
Size: 968 4580
AlwNI CAG_nnn'CTG
Cuts at: 3291 4863 3291
Size: 1572 3976
ApaLI G'TGCA_C
Cuts at: 2171 3605 4761 2171
Size: 1434 1156 2958
Fragments arranged by size:
2958 1434 1156
ApoI r'AATT_y
Cuts at: 1493 2303 2342 2607 2857 3083 3235 5344 1493
Size: 810 39 265 250 226 152 2109 1697
Fragments arranged by size:
2109 1697 810 265 250 226 152 39
AseI AT'TA_AT
Cuts at: 160 1032 4102 160
Size: 872 3070 1606
Fragments arranged by size:
3070 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2873 1870
Size: 73 930 4545
Fragments arranged by size:
4545 930 73
BamHI G'GATC_C
Cuts at: 2878 3245 2878
Size: 367 5181
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2869 4324 618
Size: 325 217 627 1082 1455 1842
Fragments arranged by size:
1842 1455 1082 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2709 2867 1391
Size: 1318 158 4072
Fragments arranged by size:
4072 1318 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2838 928
Size: 971 939 3638
Fragments arranged by size:
3638 971 939
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3739 3773 1903
Size: 34 1802 34 3678
Fragments arranged by size:
3678 1802 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3440 4650 3440
Size: 1210 4338
BclI T'GATC_A
Cuts at: 1548 2431 1548
Size: 883 4665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4159 136
Size: 107 122 71 631 605 2487 1525
Fragments arranged by size:
2487 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2436 5543 2436
Size: 3107 2441
BlpI GC'TnA_GC
Cuts at: 2528 2965 2528
Size: 437 5111
Bme1580I G_kGCm'C
Cuts at: 1790 2175 3609 4765 1790
Size: 385 1434 1156 2573
Fragments arranged by size:
2573 1434 1156 385
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5548
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3279 4237 442
Size: 416 53 83 399 24 1862 958 1753
Fragments arranged by size:
1862 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2681 4208 1239
Size: 1442 1527 2579
Fragments arranged by size:
2579 1527 1442
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2197 3673 4538 4836 2197
Size: 1476 865 298 2909
Fragments arranged by size:
2909 1476 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4211 882
Size: 617 2712 2219
Fragments arranged by size:
2712 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3339 493
Size: 711 2135 2702
Fragments arranged by size:
2702 2135 711
BsaBI GATnn'nnATC
Cuts at: 5542 5542
Size: 5548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2486 3355 3737 4372
Size: 53 83 186 936 953 869 382 635
Cuts at: 4372 275
Size: 1451
Fragments arranged by size:
1451 953 936 869 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2556 3981 4379 4653 4800 5211 2556
Size: 1425 398 274 147 411 2893
Fragments arranged by size:
2893 1425 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5323
Fragments arranged by size:
5323 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5548
BseYI C'CCAG_C
Cuts at: 4751 5247 4751
Size: 496 5052
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2072 1163
Size: 573 336 4639
Fragments arranged by size:
4639 573 336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2998 3759 4787 1540
Size: 275 186 997 761 1028 2301
Fragments arranged by size:
2301 1028 997 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2175 2709 3609 3694 4765 2175
Size: 534 900 85 1071 2958
Fragments arranged by size:
2958 1071 900 534 85
BsmI GAATG_Cn'
Cuts at: 2550 3059 3152 2550
Size: 509 93 4946
Fragments arranged by size:
4946 509 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2709 2867 3609 3694 4765 1391
Size: 399 385 534 158 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 534 399 385 158 85
BspEI T'CCGG_A
Cuts at: 2556 5211 2556
Size: 2655 2893
BspHI T'CATG_A
Cuts at: 3437 5167 3437
Size: 1730 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2903 844
Size: 2059 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3435 1394
Size: 429 1612 3507
Fragments arranged by size:
3507 1612 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2539 4043 4217 65
Size: 1183 1291 1504 174 1396
Fragments arranged by size:
1504 1396 1291 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2523 4192 4379 1700
Size: 110 713 1669 187 2869
Fragments arranged by size:
2869 1669 713 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5548
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5548
BssSI C'ACGA_G
Cuts at: 3608 4620 3608
Size: 1012 4536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5548
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2436 2878 3245 3630 3647 5099 5185 5197
Size: 464 442 367 385 17 1452 86 12
Cuts at: 5197 5543 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 464 442 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2799 2799
Size: 5548
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4057
Fragments arranged by size:
4057 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4809
Fragments arranged by size:
4809 683 56
BtsI GCAGTG_nn'
Cuts at: 3064 3858 3878 3064
Size: 794 20 4734
Fragments arranged by size:
4734 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5513
DraI TTT'AAA
Cuts at: 2852 2852
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2521 2587
Size: 54 1425 50 275 18 177 514 66
Cuts at: 2587 2995 3884 5513 8
Size: 408 889 1629 43
Fragments arranged by size:
1629 1425 889 514 408 275 177 66
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2995 1537
Size: 275 1183 4090
Fragments arranged by size:
4090 1183 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2162 2811 3478 1353
Size: 809 649 667 3423
Fragments arranged by size:
3423 809 667 649
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4135 4509 4655 387
Size: 1064 138 2546 374 146 1280
Fragments arranged by size:
2546 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2707 2707
Size: 5548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2668 2681 3611 4208 4995 5263 1239
Size: 393 1036 13 930 597 787 268 1524
Fragments arranged by size:
1524 1036 930 787 597 393 268 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2424 2424
Size: 5548
EcoRI G'AATT_C
Cuts at: 2857 3235 2857
Size: 378 5170
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2362 2362
Size: 5548
FspI TGC'GCA
Cuts at: 4054 4054
Size: 5548
HaeII r_GCGC'y
Cuts at: 1678 2414 4695 1678
Size: 736 2281 2531
Fragments arranged by size:
2531 2281 736
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373
Size: 32 135 32 2617 32 42 32 2626
Fragments arranged by size:
2626 2617 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2077 2266 2892 3138 669
Size: 988 281 139 189 626 246 3079
Fragments arranged by size:
3079 988 626 281 246 189 139
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5548
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 5548
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4662 4846 1305
Size: 3357 184 2007
Fragments arranged by size:
3357 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3726
Fragments arranged by size:
3726 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2030 2150 2727 3506 3865 4024 518
Size: 648 864 120 577 779 359 159 2042
Fragments arranged by size:
2042 864 779 648 577 359 159 120
MspA1I CmG'CkG
Cuts at: 1606 2629 2964 3641 4789 5034 1606
Size: 1023 335 677 1148 245 2120
Fragments arranged by size:
2120 1148 1023 677 335 245
NaeI GCC'GGC
Cuts at: 2525 2525
Size: 5548
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4057
Fragments arranged by size:
4057 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5548
NgoMIV G'CCGG_C
Cuts at: 2523 2523
Size: 5548
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2995 2995
Size: 5548
NspI r_CATG'y
Cuts at: 1132 1229 2155 2512 2906 4451 1132
Size: 97 926 357 394 1545 2229
Fragments arranged by size:
2229 1545 926 394 357 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5548
PciI A'CATG_T
Cuts at: 4447 4447
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2146 2629 1108
Size: 1038 483 4027
Fragments arranged by size:
4027 1038 483
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 5130 5162 3617
Size: 32 1481 32 4003
Fragments arranged by size:
4003 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5548
PsiI TTA'TAA
Cuts at: 3118 3118
Size: 5548
PstI C_TGCA'G
Cuts at: 830 2900 830
Size: 2070 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5548
PvuII CAG'CTG
Cuts at: 2629 2629
Size: 5548
SacI G_AGCT'C
Cuts at: 2709 2709
Size: 5548
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SapI GCTCTTCn'nnn_
Cuts at: 2162 2811 2162
Size: 649 4899
SbfI CC_TGCA'GG
Cuts at: 2900 2900
Size: 5548
ScaI AGT'ACT
Cuts at: 3796 5508 3796
Size: 1712 3836
SfcI C'TryA_G
Cuts at: 826 1045 2280 2896 4031 4712 4903 826
Size: 219 1235 616 1135 681 191 1471
Fragments arranged by size:
1471 1235 1135 681 616 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5548
SmaI CCC'GGG
Cuts at: 1872 2875 1872
Size: 1003 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2176 3652 4553 4815 820
Size: 197 926 233 1476 901 262 1553
Fragments arranged by size:
1553 1476 926 901 262 233 197
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2906 1132
Size: 1774 3774
SspI AAT'ATT
Cuts at: 5 52 3472 5
Size: 47 3420 2081
Fragments arranged by size:
3420 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5548
StyI C'CwwG_G
Cuts at: 513 1065 2004 2987 513
Size: 552 939 983 3074
Fragments arranged by size:
3074 983 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2249 3575 3592 3745 3930 4375 325
Size: 1255 669 1326 17 153 185 445 1498
Fragments arranged by size:
1498 1326 1255 669 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2019 3794 5506
Size: 274 80 33 51 1443 42 1775 1712
Cuts at: 5506 96
Size: 138
Fragments arranged by size:
1775 1712 1443 274 138 80 51 42
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2458 2490 5508 5540 2458
Size: 32 3018 32 2466
Fragments arranged by size:
3018 2466 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5548
XbaI T'CTAG_A
Cuts at: 2884 2884
Size: 5548
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5548
XmaI C'CCGG_G
Cuts at: 1870 2873 1870
Size: 1003 4545
XmnI GAAnn'nnTTC
Cuts at: 3677 3677
Size: 5548
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2487 3356 276
Size: 53 83 186 936 953 869 2468
Fragments arranged by size:
2468 953 936 869 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoO109I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI