Restriction map for
pFN21AB4991
(Circular) (Six-base) MAPSORT of: pFN21AB4991.seq Check: 309 from: 1 to: 5638
pFN21AB4991 5638 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2351 2981 1937
Size: 414 630 4594
Fragments arranged by size:
4594 630 414
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 2541 3701 5085 5353 1632
Size: 725 184 1160 1384 268 1917
Fragments arranged by size:
1917 1384 1160 725 268 184
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 4537 5514 1203
Size: 3334 977 1327
Fragments arranged by size:
3334 1327 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2120 1184
Size: 936 4702
AlwNI CAG_nnn'CTG
Cuts at: 3381 4953 3381
Size: 1572 4066
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2315 2947 3173 3325 5434 1493
Size: 822 632 226 152 2109 1697
Fragments arranged by size:
2109 1697 822 632 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2080 2963 1870
Size: 73 137 883 4545
Fragments arranged by size:
4545 883 137 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2460 2959 4414 618
Size: 325 217 627 673 499 1455 1842
Fragments arranged by size:
1842 1455 673 627 499 325 217
BanII G_rGCy'C
Cuts at: 1391 2674 2797 2957 1391
Size: 1283 123 160 4072
Fragments arranged by size:
4072 1283 160 123
BbeI G_GCGC'C
Cuts at: 2464 2464
Size: 5638
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3829 3863 1903
Size: 34 50 34 1808 34 3678
Fragments arranged by size:
3678 1808 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 2706 1548
Size: 1158 4480
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2801 4249 136
Size: 107 122 71 631 605 1129 1448 1525
Fragments arranged by size:
1525 1448 1129 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 3699 4855 1790
Size: 1909 1156 2573
Fragments arranged by size:
2573 1909 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2757 3369 4327
Size: 416 53 83 399 24 1340 612 958
Cuts at: 4327 442
Size: 1753
Fragments arranged by size:
1753 1340 958 612 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4298 1239
Size: 3059 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2500 2772 3763 4628 4926 2500
Size: 272 991 865 298 3212
Fragments arranged by size:
3212 991 865 298 272
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4301 882
Size: 617 2802 2219
Fragments arranged by size:
2802 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2371 3429 493
Size: 711 1167 1058 2702
Fragments arranged by size:
2702 1167 1058 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2461 3445 3827 4462
Size: 53 83 186 936 928 984 382 635
Cuts at: 4462 275
Size: 1451
Fragments arranged by size:
1451 984 936 928 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2322 2877 4071 4469 4743 4890 5301 2322
Size: 555 1194 398 274 147 411 2659
Fragments arranged by size:
2659 1194 555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2784 2814 1178
Size: 30 165 30 1381 30 4002
Fragments arranged by size:
4002 1381 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2098 2101 2682 1186
Size: 912 3 581 4142
Fragments arranged by size:
4142 912 581 3
BseYI C'CCAG_C
Cuts at: 4841 5337 4841
Size: 496 5142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5065
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 2351 3088 3849 4877 1540
Size: 275 186 33 317 737 761 1028 2301
Fragments arranged by size:
2301 1028 761 737 317 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 2268 3699 3784 4855 2268
Size: 1431 85 1071 3051
Fragments arranged by size:
3051 1431 1071 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2268 2674 2797 2957 3699 3784 4855
Size: 399 478 406 123 160 742 85 1071
Cuts at: 4855 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 478 406 399 160 123
85
BspEI T'CCGG_A
Cuts at: 2877 5301 2877
Size: 2424 3214
BspHI T'CATG_A
Cuts at: 2709 3527 5257 2709
Size: 818 1730 3090
Fragments arranged by size:
3090 1730 818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2652 2993 844
Size: 1808 341 3489
Fragments arranged by size:
3489 1808 341
BsrBI CCG'CTC
Cuts at: 1394 1823 2091 3525 1394
Size: 429 268 1434 3507
Fragments arranged by size:
3507 1434 429 268
BsrDI GCAATG_nn'
Cuts at: 65 1248 2215 2286 2439 4133 4307 65
Size: 1183 967 71 153 1694 174 1396
Fragments arranged by size:
1694 1396 1183 967 174 153 71
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 2616 96
Size: 2520 3118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2783 794
Size: 1989 3649
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2325 2968 3335 3720 3737 5189 5275 5287
Size: 353 643 367 385 17 1452 86 12
Cuts at: 5287 5633 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 643 385 367 353 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2274 513
Size: 552 1209 3877
Fragments arranged by size:
3877 1209 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 3154 3948 3968 3154
Size: 794 20 4824
Fragments arranged by size:
4824 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2564 4645 809
Size: 507 1248 2081 1802
Fragments arranged by size:
2081 1802 1248 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2596 3085 3974
Size: 54 1425 50 275 18 766 489 889
Cuts at: 3974 5603 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 766 489 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2132 2141 2840 3568 1353
Size: 779 9 699 728 3423
Fragments arranged by size:
3423 779 728 699 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 2541 3701 4298 5085 5353 1239
Size: 393 725 184 1160 597 787 268 1524
Fragments arranged by size:
1524 1160 787 725 597 393 268 184
EcoRI G'AATT_C
Cuts at: 2315 2947 3325 2315
Size: 632 378 4628
Fragments arranged by size:
4628 632 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 5638
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 2069 2464 4785 1678
Size: 391 395 2321 2531
Fragments arranged by size:
2531 2321 395 391
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4279 4311 4353 4385 1373
Size: 32 135 32 2707 32 42 32 2626
Fragments arranged by size:
2707 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2352 2982 3228 669
Size: 988 281 414 630 246 3079
Fragments arranged by size:
3079 988 630 414 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KasI G'GCGC_C
Cuts at: 2460 2460
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2598 10
Size: 54 1425 343 766 3050
Fragments arranged by size:
3050 1425 766 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2763 3596 3955 4114 518
Size: 648 1597 833 359 159 2042
Fragments arranged by size:
2042 1597 833 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3054 3731 4879 5124 1606
Size: 1448 677 1148 245 2120
Fragments arranged by size:
2120 1448 1148 677 245
NarI GG'CG_CC
Cuts at: 2461 2461
Size: 5638
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5086
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NruI TCG'CGA
Cuts at: 2300 2300
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2996 4541 1132
Size: 97 1767 1545 2229
Fragments arranged by size:
2229 1767 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5638
PfoI T'CCnGG_A
Cuts at: 2037 2568 2037
Size: 531 5107
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2649 2697 2990 830
Size: 1819 48 293 3478
Fragments arranged by size:
3478 1819 293 48
PvuI CG_AT'CG
Cuts at: 2001 2034 2001
Size: 33 5605
SalI G'TCGA_C
Cuts at: 1936 2350 2980 1936
Size: 414 630 4594
Fragments arranged by size:
4594 630 414
SbfI CC_TGCA'GG
Cuts at: 2649 2990 2649
Size: 341 5297
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SfcI C'TryA_G
Cuts at: 826 1045 2539 2645 2693 2986 4121 4802 4993
Size: 219 1494 106 48 293 1135 681 191
Cuts at: 4993 826
Size: 1471
Fragments arranged by size:
1494 1471 1135 681 293 219 191 106
48
SfoI GGC'GCC
Cuts at: 2462 2462
Size: 5638
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2479 2787 3742 4643 4905 820
Size: 197 926 536 308 955 901 262 1553
Fragments arranged by size:
1553 955 926 901 536 308 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 3077 513
Size: 552 2012 3074
Fragments arranged by size:
3074 2012 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3665 3682 3835 4020 4465 325
Size: 1255 2085 17 153 185 445 1498
Fragments arranged by size:
2085 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2616 3884 5596
Size: 274 80 33 51 1443 639 1268 1712
Cuts at: 5596 96
Size: 138
Fragments arranged by size:
1712 1443 1268 639 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2784 2816 5598 5630 2784
Size: 32 2782 32 2792
Fragments arranged by size:
2792 2782 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2770 2770
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 2585 3767 2585
Size: 1182 4456
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI
NgoMIV NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI