Restriction map for
pFN21AB4988
(Circular) (Six-base) MAPSORT of: pFN21AB4988.seq Check: 3978 from: 1 to: 5464
pFN21AB4988 5464 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3274 278
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2807 1937
Size: 870 4594
Acc65I G'GTAC_C
Cuts at: 2785 2785
Size: 5464
AclI AA'CG_TT
Cuts at: 1563 3591 3964 1563
Size: 2028 373 3063
Fragments arranged by size:
3063 2028 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3527 4911 5179 1632
Size: 1895 1384 268 1917
Fragments arranged by size:
1917 1895 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5267
AflIII A'CryG_T
Cuts at: 1203 4363 5340 1203
Size: 3160 977 1327
Fragments arranged by size:
3160 1327 977
AgeI A'CCGG_T
Cuts at: 4295 4295
Size: 5464
AhdI GACnn_n'nnGTC
Cuts at: 4193 4193
Size: 5464
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5464
AlwNI CAG_nnn'CTG
Cuts at: 2677 3207 4779 2677
Size: 530 1572 3362
Fragments arranged by size:
3362 1572 530
ApaLI G'TGCA_C
Cuts at: 3521 4677 3521
Size: 1156 4308
ApoI r'AATT_y
Cuts at: 1493 2451 2773 2999 3151 5260 1493
Size: 958 322 226 152 2109 1697
Fragments arranged by size:
2109 1697 958 322 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4018 160
Size: 872 2986 1606
Fragments arranged by size:
2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
AvaI C'yCGr_G
Cuts at: 1870 1943 2789 1870
Size: 73 846 4545
Fragments arranged by size:
4545 846 73
BamHI G'GATC_C
Cuts at: 2794 3161 2794
Size: 367 5097
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2785 4240 618
Size: 325 217 627 998 1455 1842
Fragments arranged by size:
1842 1455 998 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2191 2739 2783 1391
Size: 800 548 44 4072
Fragments arranged by size:
4072 800 548 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 928
Size: 971 199 4294
Fragments arranged by size:
4294 971 199
BbvCI CC'TCA_GC
Cuts at: 2586 2586
Size: 5464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2564 2598 3655 3689 1903
Size: 34 627 34 1057 34 3678
Fragments arranged by size:
3678 1057 627 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3356 4566 3356
Size: 1210 4254
BclI T'GATC_A
Cuts at: 1548 2172 1548
Size: 624 4840
BfrBI ATG'CAT
Cuts at: 2635 2635
Size: 5464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2016 2654 4075
Size: 107 122 71 631 605 344 638 1421
Cuts at: 4075 136
Size: 1525
Fragments arranged by size:
1525 1421 638 631 605 344 122 107
71
BglII A'GATC_T
Cuts at: 5459 5459
Size: 5464
BlpI GC'TnA_GC
Cuts at: 2881 2881
Size: 5464
Bme1580I G_kGCm'C
Cuts at: 1790 3525 4681 1790
Size: 1735 1156 2573
Fragments arranged by size:
2573 1735 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5464
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2479 3195 4153
Size: 416 53 83 399 24 1062 716 958
Cuts at: 4153 442
Size: 1753
Fragments arranged by size:
1753 1062 958 716 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2409 1056
Size: 1353 4111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2532 2532
Size: 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4124 1239
Size: 2885 2579
Bpu10I CC'TnA_GC
Cuts at: 2586 2586
Size: 5464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3589 4454 4752 3589
Size: 865 298 4301
Fragments arranged by size:
4301 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4127 882
Size: 617 2628 2219
Fragments arranged by size:
2628 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3255 493
Size: 711 2051 2702
Fragments arranged by size:
2702 2051 711
BsaBI GATnn'nnATC
Cuts at: 5458 5458
Size: 5464
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3271 3653 4288 275
Size: 53 83 186 936 1738 382 635 1451
Fragments arranged by size:
1738 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3897 4295 4569 4716 5127 3897
Size: 398 274 147 411 4234
Fragments arranged by size:
4234 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2088 2118 1178
Size: 30 165 30 685 30 4524
Fragments arranged by size:
4524 685 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2017 2729 1186
Size: 831 712 3921
Fragments arranged by size:
3921 831 712
BseYI C'CCAG_C
Cuts at: 2677 4667 5163 2677
Size: 1990 496 2978
Fragments arranged by size:
2978 1990 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2728 1163
Size: 573 992 3899
Fragments arranged by size:
3899 992 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2914 3675 4703 1540
Size: 275 186 913 761 1028 2301
Fragments arranged by size:
2301 1028 913 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2739 3525 3610 4681 2739
Size: 786 85 1071 3522
Fragments arranged by size:
3522 1071 786 85
BsmI GAATG_Cn'
Cuts at: 2358 2975 3068 2358
Size: 617 93 4754
Fragments arranged by size:
4754 617 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2191 2739 2783 3525 3610 4681 1391
Size: 399 401 548 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 548 401 399 85 44
BspEI T'CCGG_A
Cuts at: 5127 5127
Size: 5464
BspHI T'CATG_A
Cuts at: 2628 3353 5083 2628
Size: 725 1730 3009
Fragments arranged by size:
3009 1730 725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2819 844
Size: 1975 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3351 1394
Size: 429 1528 3507
Fragments arranged by size:
3507 1528 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3959 4133 65
Size: 1183 2711 174 1396
Fragments arranged by size:
2711 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4108 4295 1700
Size: 110 2298 187 2869
Fragments arranged by size:
2869 2298 187 110
BsrGI T'GTAC_A
Cuts at: 96 2148 2425 96
Size: 2052 277 3135
Fragments arranged by size:
3135 2052 277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5464
BssSI C'ACGA_G
Cuts at: 3524 4536 3524
Size: 1012 4452
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2085 794
Size: 1291 4173
BstBI TT'CG_AA
Cuts at: 4268 4268
Size: 5464
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5464
BstYI r'GATC_y
Cuts at: 1972 2722 2794 3161 3546 3563 5015 5101 5113
Size: 750 72 367 385 17 1452 86 12
Cuts at: 5113 5459 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 750 385 367 346 86 72
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3973
Fragments arranged by size:
3973 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4725
Fragments arranged by size:
4725 683 56
BtsI GCAGTG_nn'
Cuts at: 2980 3774 3794 2980
Size: 794 20 4650
Fragments arranged by size:
4650 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5429
DraI TTT'AAA
Cuts at: 2768 2768
Size: 5464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4471 809
Size: 507 3155 1802
Fragments arranged by size:
3155 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2911 3800 5429
Size: 54 1425 50 275 18 1081 889 1629
Cuts at: 5429 8
Size: 43
Fragments arranged by size:
1629 1425 1081 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2911 1537
Size: 275 1099 4090
Fragments arranged by size:
4090 1099 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3394 1353
Size: 2041 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2766 4051 4425 4571 387
Size: 1064 138 1177 1285 374 146 1280
Fragments arranged by size:
1285 1280 1177 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2737 2737
Size: 5464
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3527 4124 4911 5179 1239
Size: 393 1895 597 787 268 1524
Fragments arranged by size:
1895 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2451 2773 3151 2451
Size: 322 378 4764
Fragments arranged by size:
4764 378 322
FspI TGC'GCA
Cuts at: 3970 3970
Size: 5464
HaeII r_GCGC'y
Cuts at: 1678 4611 1678
Size: 2933 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2088 2120 2707 2739 4105
Size: 32 135 32 516 32 587 32 1366
Cuts at: 4105 4137 4179 4211 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1366 587 516 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2808 3054 669
Size: 988 281 870 246 3079
Fragments arranged by size:
3079 988 870 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5464
HpaI GTT'AAC
Cuts at: 3054 3054
Size: 5464
KpnI G_GTAC'C
Cuts at: 2789 2789
Size: 5464
MfeI C'AATT_G
Cuts at: 2545 3063 2545
Size: 518 4946
MluI A'CGCG_T
Cuts at: 5340 5340
Size: 5464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2598 4578 4762 1305
Size: 1293 1980 184 2007
Fragments arranged by size:
2007 1980 1293 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3642
Fragments arranged by size:
3642 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2445 3422 3781 3940 518
Size: 648 1279 977 359 159 2042
Fragments arranged by size:
2042 1279 977 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2075 2437 2674 2880 3557 4705 4950 1606
Size: 469 362 237 206 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 469 362 245 237 206
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3973
Fragments arranged by size:
3973 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5464
NheI G'CTAG_C
Cuts at: 1052 2405 1052
Size: 1353 4111
NotI GC'GGCC_GC
Cuts at: 2911 2911
Size: 5464
NsiI A_TGCA'T
Cuts at: 2637 2637
Size: 5464
NspI r_CATG'y
Cuts at: 1132 1229 2639 2686 2822 4367 1132
Size: 97 1410 47 136 1545 2229
Fragments arranged by size:
2229 1545 1410 136 97 47
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5464
PciI A'CATG_T
Cuts at: 4363 4363
Size: 5464
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5464
PfoI T'CCnGG_A
Cuts at: 2232 2232
Size: 5464
PmeI GTTT'AAAC
Cuts at: 2768 2768
Size: 5464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 5046 5078 3533
Size: 32 1481 32 3919
Fragments arranged by size:
3919 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5464
PsiI TTA'TAA
Cuts at: 3034 3034
Size: 5464
PstI C_TGCA'G
Cuts at: 830 2531 2816 830
Size: 1701 285 3478
Fragments arranged by size:
3478 1701 285
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5464
PvuII CAG'CTG
Cuts at: 2075 2437 2674 2075
Size: 362 237 4865
Fragments arranged by size:
4865 362 237
SacI G_AGCT'C
Cuts at: 2739 2739
Size: 5464
SalI G'TCGA_C
Cuts at: 1936 2806 1936
Size: 870 4594
SbfI CC_TGCA'GG
Cuts at: 2816 2816
Size: 5464
ScaI AGT'ACT
Cuts at: 3712 5424 3712
Size: 1712 3752
SfcI C'TryA_G
Cuts at: 826 1045 2166 2181 2527 2812 3947 4628 4819
Size: 219 1121 15 346 285 1135 681 191
Cuts at: 4819 826
Size: 1471
Fragments arranged by size:
1471 1135 1121 681 346 285 219 191
15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5464
SmaI CCC'GGG
Cuts at: 1872 2791 1872
Size: 919 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3568 4469 4731 820
Size: 197 926 1625 901 262 1553
Fragments arranged by size:
1625 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3255 493
Size: 2762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5464
SphI G_CATG'C
Cuts at: 1132 2686 2822 1132
Size: 1554 136 3774
Fragments arranged by size:
3774 1554 136
SspI AAT'ATT
Cuts at: 5 52 3388 5
Size: 47 3336 2081
Fragments arranged by size:
3336 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5464
StyI C'CwwG_G
Cuts at: 513 1065 2004 2903 513
Size: 552 939 899 3074
Fragments arranged by size:
3074 939 899 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3491 3508 3661 3846 4291 325
Size: 1255 1911 17 153 185 445 1498
Fragments arranged by size:
1911 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 2425 3710
Size: 274 80 33 51 1443 171 277 1285
Cuts at: 3710 5422 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1285 277 274 171 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5424 5456 5424
Size: 32 5432
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5464
XbaI T'CTAG_A
Cuts at: 2800 2800
Size: 5464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2038
Size: 5464
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5464
XmaI C'CCGG_G
Cuts at: 1870 2789 1870
Size: 919 4545
XmnI GAAnn'nnTTC
Cuts at: 3593 3593
Size: 5464
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3272 276
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI