Restriction map for
pFN21AB4987
(Circular) (Six-base) MAPSORT of: pFN21AB4987.seq Check: 3182 from: 1 to: 6118
pFN21AB4987 6118 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2973 3928 278
Size: 53 83 186 936 1437 955 2468
Fragments arranged by size:
2468 1437 955 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3461 1937
Size: 1524 4594
Acc65I G'GTAC_C
Cuts at: 3439 3439
Size: 6118
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2265 2523 2854 4181 5565 5833 1632
Size: 633 258 331 1327 1384 268 1917
Fragments arranged by size:
1917 1384 1327 633 331 268 258
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 3195 5017 5994 1203
Size: 1992 1822 977 1327
Fragments arranged by size:
1992 1822 1327 977
AgeI A'CCGG_T
Cuts at: 3284 4949 3284
Size: 1665 4453
AhdI GACnn_n'nnGTC
Cuts at: 4847 4847
Size: 6118
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6118
AlwNI CAG_nnn'CTG
Cuts at: 2243 2823 3861 5433 2243
Size: 580 1038 1572 2928
Fragments arranged by size:
2928 1572 1038 580
ApaI G_GGCC'C
Cuts at: 2495 2495
Size: 6118
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 3427 3653 3805 5914 1493
Size: 1934 226 152 2109 1697
Fragments arranged by size:
2109 1934 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 3224 3242 3443 1870
Size: 73 1281 18 201 4545
Fragments arranged by size:
4545 1281 201 73 18
BamHI G'GATC_C
Cuts at: 2944 3448 3815 2944
Size: 504 367 5247
Fragments arranged by size:
5247 504 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3038 3439 4894 618
Size: 325 217 627 1251 401 1455 1842
Fragments arranged by size:
1842 1455 1251 627 401 325 217
BanII G_rGCy'C
Cuts at: 1391 2022 2070 2495 2738 3294 3437 1391
Size: 631 48 425 243 556 143 4072
Fragments arranged by size:
4072 631 556 425 243 143 48
BbeI G_GCGC'C
Cuts at: 3042 3042
Size: 6118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4309 4343 1903
Size: 34 50 34 2288 34 3678
Fragments arranged by size:
3678 2288 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4010 5220 4010
Size: 1210 4908
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6118
BfrBI ATG'CAT
Cuts at: 2512 2512
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2855 4729 136
Size: 107 122 71 631 605 1183 1874 1525
Fragments arranged by size:
1874 1525 1183 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2889 6113 2889
Size: 3224 2894
BlpI GC'TnA_GC
Cuts at: 3535 3535
Size: 6118
Bme1580I G_kGCm'C
Cuts at: 1790 2495 2574 4179 5335 1790
Size: 705 79 1605 1156 2573
Fragments arranged by size:
2573 1605 1156 705 79
BmgBI CAC'GTC
Cuts at: 1318 3196 1318
Size: 1878 4240
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3849 4807 442
Size: 416 53 83 399 24 2432 958 1753
Fragments arranged by size:
2432 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3296 3296
Size: 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2178 2537 3159 4778 1239
Size: 815 124 359 622 1619 2579
Fragments arranged by size:
2579 1619 815 622 359 124
Bpu10I CC'TnA_GC
Cuts at: 2737 2737
Size: 6118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3278 4243 5108 5406 3278
Size: 965 865 298 3990
Fragments arranged by size:
3990 965 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4781 882
Size: 617 3282 2219
Fragments arranged by size:
3282 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3909 493
Size: 711 2705 2702
Fragments arranged by size:
2705 2702 711
BsaBI GATnn'nnATC
Cuts at: 2433 6112 2433
Size: 3679 2439
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2970 3039 3925 4307
Size: 53 83 186 936 1437 69 886 382
Cuts at: 4307 4942 275
Size: 635 1451
Fragments arranged by size:
1451 1437 936 886 635 382 186 83
69 53
BsaWI w'CCGG_w
Cuts at: 3284 4551 4949 5223 5370 5781 3284
Size: 1267 398 274 147 411 3621
Fragments arranged by size:
3621 1267 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3056 3086 1178
Size: 30 165 30 1653 30 4210
Fragments arranged by size:
4210 1653 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3064 3173 1186
Size: 1878 109 4131
Fragments arranged by size:
4131 1878 109
BseYI C'CCAG_C
Cuts at: 2203 3025 5321 5817 2203
Size: 822 2296 496 2504
Fragments arranged by size:
2504 2296 822 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5545
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3568 4329 5357 1540
Size: 275 186 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2738 3165 3294 3339 4179 4264 5335 2070
Size: 668 427 129 45 840 85 1071 2853
Fragments arranged by size:
2853 1071 840 668 427 129 85 45
BsmI GAATG_Cn'
Cuts at: 2662 3629 3722 2662
Size: 967 93 5058
Fragments arranged by size:
5058 967 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2070 2495 2574 2738 3165 3294
Size: 399 232 48 425 79 164 427 129
Cuts at: 3294 3339 3437 4179 4264 5335 1391
Size: 45 98 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 427 425 399 232 164
129 98 85 79 48 45
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 3384 4007 5737 3384
Size: 623 1730 3765
Fragments arranged by size:
3765 1730 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3473 844
Size: 2629 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4005 1394
Size: 429 2182 3507
Fragments arranged by size:
3507 2182 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2514 4613 4787 65
Size: 1183 1266 2099 174 1396
Fragments arranged by size:
2099 1396 1266 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2375 3284 4762 4949 1700
Size: 110 565 909 1478 187 2869
Fragments arranged by size:
2869 1478 909 565 187 110
BsrGI T'GTAC_A
Cuts at: 96 2478 96
Size: 2382 3736
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 4178 5190 4178
Size: 1012 5106
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6118
BstBI TT'CG_AA
Cuts at: 4922 4922
Size: 6118
BstEII G'GTnAC_C
Cuts at: 1184 2519 1184
Size: 1335 4783
BstYI r'GATC_y
Cuts at: 1972 2889 2944 3448 3815 4200 4217 5669 5755
Size: 917 55 504 367 385 17 1452 86
Cuts at: 5755 5767 6113 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 917 504 385 367 346 86
55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2163 2264 513
Size: 552 1098 101 4367
Fragments arranged by size:
4367 1098 552 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2035 2656 487
Size: 683 56 809 621 3949
Fragments arranged by size:
3949 809 683 621 56
BtsI GCAGTG_nn'
Cuts at: 2290 3634 4428 4448 2290
Size: 1344 794 20 3960
Fragments arranged by size:
3960 1344 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 3422 3422
Size: 6118
DraIII CAC_nnn'GTG
Cuts at: 2547 2547
Size: 6118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5125 809
Size: 507 3809 1802
Fragments arranged by size:
3809 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2377 3096 3565
Size: 54 1425 50 275 18 547 719 469
Cuts at: 3565 4454 6083 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 719 547 469 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2241 2503 4048 1353
Size: 888 262 1545 3423
Fragments arranged by size:
3423 1545 888 262
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4705 5079 5225 387
Size: 1064 138 3116 374 146 1280
Fragments arranged by size:
3116 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2068 2736 3292 2068
Size: 668 556 4894
Fragments arranged by size:
4894 668 556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2178 2265 2523 2537 2854 3159
Size: 393 422 124 87 258 14 317 305
Cuts at: 3159 4181 4778 5565 5833 1239
Size: 1022 597 787 268 1524
Fragments arranged by size:
1524 1022 787 597 422 393 317 305
268 258 124 87 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2630 2630
Size: 6118
EcoO109I rG'GnC_Cy
Cuts at: 2076 2491 2492 2617 2076
Size: 415 1 125 5577
Fragments arranged by size:
5577 415 125 1
EcoRI G'AATT_C
Cuts at: 3427 3805 3427
Size: 378 5740
EcoRV GAT'ATC
Cuts at: 2911 2911
Size: 6118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3421 3421
Size: 6118
FspI TGC'GCA
Cuts at: 2402 4624 2402
Size: 2222 3896
HaeII r_GCGC'y
Cuts at: 1678 3042 5265 1678
Size: 1364 2223 2531
Fragments arranged by size:
2531 2223 1364
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2974 3006 3056
Size: 32 135 32 437 32 933 32 50
Cuts at: 3056 3088 3138 3170 4759 4791 4833 4865 1373
Size: 32 50 32 1589 32 42 32 2626
Fragments arranged by size:
2626 1589 933 437 135 50 50 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3462 3708 669
Size: 988 281 1524 246 3079
Fragments arranged by size:
3079 1524 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6118
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KasI G'GCGC_C
Cuts at: 3038 3038
Size: 6118
KpnI G_GTAC'C
Cuts at: 3443 3443
Size: 6118
MfeI C'AATT_G
Cuts at: 3717 3717
Size: 6118
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5232 5416 1305
Size: 3927 184 2007
Fragments arranged by size:
3927 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4296
Fragments arranged by size:
4296 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 2729 4076 4435 4594 518
Size: 648 1419 144 1347 359 159 2042
Fragments arranged by size:
2042 1419 1347 648 359 159 144
MspA1I CmG'CkG
Cuts at: 1606 2165 3270 3534 4211 5359 5604 1606
Size: 559 1105 264 677 1148 245 2120
Fragments arranged by size:
2120 1148 1105 677 559 264 245
NarI GG'CG_CC
Cuts at: 3039 3039
Size: 6118
NcoI C'CATG_G
Cuts at: 513 1065 2264 513
Size: 552 1199 4367
Fragments arranged by size:
4367 1199 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NsiI A_TGCA'T
Cuts at: 2514 2514
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 2516 2777 3131 3476 5021 1132
Size: 97 1287 261 354 345 1545 2229
Fragments arranged by size:
2229 1545 1287 354 345 261 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6118
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6118
PfoI T'CCnGG_A
Cuts at: 2553 2553
Size: 6118
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2076 2617 2076
Size: 541 5577
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6118
PsiI TTA'TAA
Cuts at: 3688 3688
Size: 6118
PspOMI G'GGCC_C
Cuts at: 2491 2491
Size: 6118
PspXI vC'TCGA_Gb
Cuts at: 3224 3242 3224
Size: 18 6100
PstI C_TGCA'G
Cuts at: 830 2122 3036 3321 3470 830
Size: 1292 914 285 149 3478
Fragments arranged by size:
3478 1292 914 285 149
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
SacI G_AGCT'C
Cuts at: 2070 2738 3294 2070
Size: 668 556 4894
Fragments arranged by size:
4894 668 556
SacII CC_GC'GG
Cuts at: 2166 2166
Size: 6118
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SbfI CC_TGCA'GG
Cuts at: 3036 3470 3036
Size: 434 5684
ScaI AGT'ACT
Cuts at: 4366 6078 4366
Size: 1712 4406
SfcI C'TryA_G
Cuts at: 826 1045 2118 2638 3032 3317 3466 4601 5282
Size: 219 1073 520 394 285 149 1135 681
Cuts at: 5282 5473 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1073 681 520 394 285 219
191 149
SfoI GGC'GCC
Cuts at: 3040 3040
Size: 6118
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 3284 1700
Size: 1584 4534
SmaI CCC'GGG
Cuts at: 1872 3445 1872
Size: 1573 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3224 3242 3293 4222 5123 5385
Size: 197 926 1281 18 51 929 901 262
Cuts at: 5385 820
Size: 1553
Fragments arranged by size:
1553 1281 929 926 901 262 197 51
18
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 3131 3476 1132
Size: 1999 345 3774
Fragments arranged by size:
3774 1999 345
SspI AAT'ATT
Cuts at: 5 52 4042 5
Size: 47 3990 2081
Fragments arranged by size:
3990 2081 47
StuI AGG'CCT
Cuts at: 1517 2636 1517
Size: 1119 4999
StyI C'CwwG_G
Cuts at: 513 1065 2264 2574 2661 3114 3557 513
Size: 552 1199 310 87 453 443 3074
Fragments arranged by size:
3074 1199 552 453 443 310 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4145 4162 4315 4500 4945 325
Size: 1255 2565 17 153 185 445 1498
Fragments arranged by size:
2565 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2272 2478 4364
Size: 274 80 33 51 1443 295 206 1886
Cuts at: 4364 6076 96
Size: 1712 138
Fragments arranged by size:
1886 1712 1443 295 274 206 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2379 2411 2877 2900 2909 2932 6078 6110 2379
Size: 32 466 23 9 23 3146 32 2387
Fragments arranged by size:
3146 2387 466 32 32 23 23 9
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6118
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XhoI C'TCGA_G
Cuts at: 1943 3224 3242 1943
Size: 1281 18 4819
Fragments arranged by size:
4819 1281 18
XmaI C'CCGG_G
Cuts at: 1870 3443 1870
Size: 1573 4545
XmnI GAAnn'nnTTC
Cuts at: 3380 4247 3380
Size: 867 5251
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2971 3926 276
Size: 53 83 186 936 1437 955 2468
Fragments arranged by size:
2468 1437 955 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI SacI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BsiWI
BsmBI BstXI BstZ17I Bsu36I FseI FspAI NaeI NgoMIV
NruI PacI PmlI PsrI PvuII RsrII SanDI SapI
SexAI SfiI SrfI SwaI XcmI