Restriction map for
pFN21AB4982
(Circular) (Six-base) MAPSORT of: pFN21AB4982.seq Check: 5982 from: 1 to: 7108
pFN21AB4982 7108 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4918 278
Size: 53 83 186 936 3382 2468
Fragments arranged by size:
3382 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4451 1937
Size: 2514 4594
Acc65I G'GTAC_C
Cuts at: 4429 4429
Size: 7108
AclI AA'CG_TT
Cuts at: 1563 2592 5235 5608 1563
Size: 1029 2643 373 3063
Fragments arranged by size:
3063 2643 1029 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2421 3027 5171 6555 6823 1632
Size: 789 606 2144 1384 268 1917
Fragments arranged by size:
2144 1917 1384 789 606 268
AflII C'TTAA_G
Cuts at: 820 1017 4025 820
Size: 197 3008 3903
Fragments arranged by size:
3903 3008 197
AflIII A'CryG_T
Cuts at: 1203 6007 6984 1203
Size: 4804 977 1327
Fragments arranged by size:
4804 1327 977
AgeI A'CCGG_T
Cuts at: 5939 5939
Size: 7108
AhdI GACnn_n'nnGTC
Cuts at: 5837 5837
Size: 7108
AleI CACnn'nnGTG
Cuts at: 1166 3411 3861 1166
Size: 2245 450 4413
Fragments arranged by size:
4413 2245 450
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2099 1184
Size: 915 6193
AlwNI CAG_nnn'CTG
Cuts at: 2747 4337 4851 6423 2747
Size: 1590 514 1572 3432
Fragments arranged by size:
3432 1590 1572 514
ApaI G_GGCC'C
Cuts at: 4269 4269
Size: 7108
ApaLI G'TGCA_C
Cuts at: 5165 6321 5165
Size: 1156 5952
ApoI r'AATT_y
Cuts at: 1493 3422 3502 4417 4643 4795 6904 1493
Size: 1929 80 915 226 152 2109 1697
Fragments arranged by size:
2109 1929 1697 915 226 152 80
AseI AT'TA_AT
Cuts at: 160 1032 5662 160
Size: 872 4630 1606
Fragments arranged by size:
4630 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7108
AvaI C'yCGr_G
Cuts at: 1870 1943 4433 1870
Size: 73 2490 4545
Fragments arranged by size:
4545 2490 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3931 3964 3931
Size: 33 7075
BamHI G'GATC_C
Cuts at: 4438 4805 4438
Size: 367 6741
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2525 4429 5884 618
Size: 325 217 627 738 1904 1455 1842
Fragments arranged by size:
1904 1842 1455 738 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2192 2796 4269 4427 1391
Size: 801 604 1473 158 4072
Fragments arranged by size:
4072 1473 801 604 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2528 3090 4356 928
Size: 971 629 562 1266 3680
Fragments arranged by size:
3680 1266 971 629 562
BbvCI CC'TCA_GC
Cuts at: 2969 2969
Size: 7108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5299 5333 1903
Size: 34 50 34 3278 34 3678
Fragments arranged by size:
3678 3278 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5000 6210 5000
Size: 1210 5898
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7108
BfrBI ATG'CAT
Cuts at: 2008 2776 2008
Size: 768 6340
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5719 136
Size: 107 122 71 631 605 4047 1525
Fragments arranged by size:
4047 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4324 7103 4324
Size: 2779 4329
BlpI GC'TnA_GC
Cuts at: 4525 4525
Size: 7108
Bme1580I G_kGCm'C
Cuts at: 1790 2530 2857 3791 4269 5169 6325 1790
Size: 740 327 934 478 900 1156 2573
Fragments arranged by size:
2573 1156 934 900 740 478 327
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7108
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2150 2538 4020
Size: 416 53 83 399 24 733 388 1482
Cuts at: 4020 4839 5797 442
Size: 819 958 1753
Fragments arranged by size:
1753 1482 958 819 733 416 399 388
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7108
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4327 4327
Size: 7108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 2674 2810 3312 4007 4258 5768 1239
Size: 1085 350 136 502 695 251 1510 2579
Fragments arranged by size:
2579 1510 1085 695 502 350 251 136
Bpu10I CC'TnA_GC
Cuts at: 2186 2969 2186
Size: 783 6325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3899 4055 4080 4159 5233 6098 6396 3899
Size: 156 25 79 1074 865 298 4611
Fragments arranged by size:
4611 1074 865 298 156 79 25
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5771 882
Size: 617 4272 2219
Fragments arranged by size:
4272 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4081 4899 493
Size: 711 2877 818 2702
Fragments arranged by size:
2877 2702 818 711
BsaBI GATnn'nnATC
Cuts at: 2451 3636 7102 2451
Size: 1185 3466 2457
Fragments arranged by size:
3466 2457 1185
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4915 5297 5932 275
Size: 53 83 186 936 3382 382 635 1451
Fragments arranged by size:
3382 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5541 5939 6213 6360 6771 5541
Size: 398 274 147 411 5878
Fragments arranged by size:
5878 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2266 2296 2918 2948 3261
Size: 30 165 30 863 30 622 30 313
Cuts at: 3261 3291 1178
Size: 30 4995
Fragments arranged by size:
4995 863 622 313 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2651 2983 4307 1186
Size: 1465 332 1324 3987
Fragments arranged by size:
3987 1465 1324 332
BseYI C'CCAG_C
Cuts at: 3893 6311 6807 3893
Size: 2418 496 4194
Fragments arranged by size:
4194 2418 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3526 4383 1163
Size: 573 1790 857 3888
Fragments arranged by size:
3888 1790 857 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4558 5319 6347 1540
Size: 275 186 2557 761 1028 2301
Fragments arranged by size:
2557 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2192 2796 2891 3782 4086 5169 5254 6325 2192
Size: 604 95 891 304 1083 85 1071 2975
Fragments arranged by size:
2975 1083 1071 891 604 304 95 85
BsmI GAATG_Cn'
Cuts at: 2668 4619 4712 2668
Size: 1951 93 5064
Fragments arranged by size:
5064 1951 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2192 2530 2796 2857 2891 3782 3791
Size: 399 402 338 266 61 34 891 9
Cuts at: 3791 4086 4269 4427 5169 5254 6325 1391
Size: 295 183 158 742 85 1071 2174
Fragments arranged by size:
2174 1071 891 742 402 399 338 295
266 183 158 85 61 34 9
BspEI T'CCGG_A
Cuts at: 6771 6771
Size: 7108
BspHI T'CATG_A
Cuts at: 2698 3520 4174 4997 6727 2698
Size: 822 654 823 1730 3079
Fragments arranged by size:
3079 1730 823 822 654
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3909 4265 4463 844
Size: 3065 356 198 3489
Fragments arranged by size:
3489 3065 356 198
BsrBI CCG'CTC
Cuts at: 1394 1823 4995 1394
Size: 429 3172 3507
Fragments arranged by size:
3507 3172 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2083 2820 5603 5777 65
Size: 1183 835 737 2783 174 1396
Fragments arranged by size:
2783 1396 1183 835 737 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5752 5939 1700
Size: 110 3942 187 2869
Fragments arranged by size:
3942 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4208 96
Size: 4112 2996
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7108
BssSI C'ACGA_G
Cuts at: 5168 6180 5168
Size: 1012 6096
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3660 4081 794
Size: 2866 421 3821
Fragments arranged by size:
3821 2866 421
BstBI TT'CG_AA
Cuts at: 5912 5912
Size: 7108
BstEII G'GTnAC_C
Cuts at: 1184 2256 2602 1184
Size: 1072 346 5690
Fragments arranged by size:
5690 1072 346
BstXI CCAn_nnnn'nTGG
Cuts at: 2963 2963
Size: 7108
BstYI r'GATC_y
Cuts at: 1972 2446 4324 4438 4805 5190 5207 6659 6745
Size: 474 1878 114 367 385 17 1452 86
Cuts at: 6745 6757 7103 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1878 1452 474 385 367 346 114
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3351 3742 513
Size: 552 2286 391 3879
Fragments arranged by size:
3879 2286 552 391
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6369
Fragments arranged by size:
6369 683 56
BtsI GCAGTG_nn'
Cuts at: 3071 4624 5418 5438 3071
Size: 1553 794 20 4741
Fragments arranged by size:
4741 1553 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7108
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7073
DraI TTT'AAA
Cuts at: 4412 4412
Size: 7108
DraIII CAC_nnn'GTG
Cuts at: 2525 2878 2525
Size: 353 6755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6115 809
Size: 507 4799 1802
Fragments arranged by size:
4799 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2551 2880 3334
Size: 54 1425 50 275 18 721 329 454
Cuts at: 3334 4555 5444 7073 8
Size: 1221 889 1629 43
Fragments arranged by size:
1629 1425 1221 889 721 454 329 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4555 1537
Size: 275 2743 4090
Fragments arranged by size:
4090 2743 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3489 4296 4311 5038 1353
Size: 2136 807 15 727 3423
Fragments arranged by size:
3423 2136 807 727 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5695 6069 6215 387
Size: 1064 138 4106 374 146 1280
Fragments arranged by size:
4106 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2190 2794 2190
Size: 604 6504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2421 2674 2810 3027 3312 4007
Size: 393 692 97 253 136 217 285 695
Cuts at: 4007 4258 5171 5768 6555 6823 1239
Size: 251 913 597 787 268 1524
Fragments arranged by size:
1524 913 787 695 692 597 393 285
268 253 251 217 136 97
EcoNI CCTnn'n_nnAGG
Cuts at: 2145 2145
Size: 7108
EcoO109I rG'GnC_Cy
Cuts at: 2177 4066 4265 2177
Size: 1889 199 5020
Fragments arranged by size:
5020 1889 199
EcoRI G'AATT_C
Cuts at: 3422 4417 4795 3422
Size: 995 378 5735
Fragments arranged by size:
5735 995 378
EcoRV GAT'ATC
Cuts at: 3556 3556
Size: 7108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4153 4153
Size: 7108
FspI TGC'GCA
Cuts at: 3476 5614 3476
Size: 2138 4970
HaeII r_GCGC'y
Cuts at: 1678 6255 1678
Size: 4577 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2492 2524 4099 4131 4132
Size: 32 135 32 920 32 1575 32 1
Cuts at: 4132 4164 5749 5781 5823 5855 1373
Size: 32 1585 32 42 32 2626
Fragments arranged by size:
2626 1585 1575 920 135 42 32 32
32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3385 4452 4698 669
Size: 988 281 1447 1067 246 3079
Fragments arranged by size:
3079 1447 1067 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3795 748
Size: 3047 4061
HpaI GTT'AAC
Cuts at: 3385 4698 3385
Size: 1313 5795
KpnI G_GTAC'C
Cuts at: 4433 4433
Size: 7108
MfeI C'AATT_G
Cuts at: 3261 4707 3261
Size: 1446 5662
MluI A'CGCG_T
Cuts at: 6984 6984
Size: 7108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2452 6222 6406 1305
Size: 1147 3770 184 2007
Fragments arranged by size:
3770 2007 1147 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2553 2882 3336 10
Size: 54 1425 343 721 329 454 3782
Fragments arranged by size:
3782 1425 721 454 343 329 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2544 2760 2781 3033 3305 3411 3630
Size: 648 1378 216 21 252 272 106 219
Cuts at: 3630 3845 3861 5066 5425 5584 518
Size: 215 16 1205 359 159 2042
Fragments arranged by size:
2042 1378 1205 648 359 272 252 219
216 215 159 106 21 16
MspA1I CmG'CkG
Cuts at: 1606 2056 4382 4524 5201 6349 6594 1606
Size: 450 2326 142 677 1148 245 2120
Fragments arranged by size:
2326 2120 1148 677 450 245 142
NcoI C'CATG_G
Cuts at: 513 1065 3351 3742 513
Size: 552 2286 391 3879
Fragments arranged by size:
3879 2286 552 391
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7108
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7108
NotI GC'GGCC_GC
Cuts at: 4555 4555
Size: 7108
NsiI A_TGCA'T
Cuts at: 2010 2778 2010
Size: 768 6340
NspI r_CATG'y
Cuts at: 1132 1229 2845 4466 6011 1132
Size: 97 1616 1621 1545 2229
Fragments arranged by size:
2229 1621 1616 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3724 1348
Size: 2376 4732
PciI A'CATG_T
Cuts at: 6007 6007
Size: 7108
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2560 3832 1108
Size: 1452 1272 4384
Fragments arranged by size:
4384 1452 1272
PmeI GTTT'AAAC
Cuts at: 4412 4412
Size: 7108
PmlI CAC'GTG
Cuts at: 4081 4081
Size: 7108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5177 5209 6690 6722 5177
Size: 32 1481 32 5563
Fragments arranged by size:
5563 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2177 4066 2177
Size: 1889 5219
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7108
PsiI TTA'TAA
Cuts at: 4678 4678
Size: 7108
PspOMI G'GGCC_C
Cuts at: 4265 4265
Size: 7108
PstI C_TGCA'G
Cuts at: 830 4460 830
Size: 3630 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7108
PvuII CAG'CTG
Cuts at: 2056 2056
Size: 7108
SacI G_AGCT'C
Cuts at: 2192 2796 2192
Size: 604 6504
SalI G'TCGA_C
Cuts at: 1936 4450 1936
Size: 2514 4594
SanDI GG'GwC_CC
Cuts at: 2177 2177
Size: 7108
SapI GCTCTTCn'nnn_
Cuts at: 3489 3489
Size: 7108
SbfI CC_TGCA'GG
Cuts at: 4460 4460
Size: 7108
ScaI AGT'ACT
Cuts at: 5356 7068 5356
Size: 1712 5396
SfcI C'TryA_G
Cuts at: 826 1045 2748 3871 3940 4354 4456 5591 6272
Size: 219 1703 1123 69 414 102 1135 681
Cuts at: 6272 6463 826
Size: 191 1471
Fragments arranged by size:
1703 1471 1135 1123 681 414 219 191
102 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7108
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7108
SmaI CCC'GGG
Cuts at: 1872 4435 1872
Size: 2563 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3914 4025 4070 4095 4138 5212
Size: 197 926 1971 111 45 25 43 1074
Cuts at: 5212 6113 6375 820
Size: 901 262 1553
Fragments arranged by size:
1971 1553 1074 926 901 262 197 111
45 43 25
SnaBI TAC'GTA
Cuts at: 493 4899 493
Size: 4406 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7108
SphI G_CATG'C
Cuts at: 1132 4466 1132
Size: 3334 3774
SspI AAT'ATT
Cuts at: 5 52 5032 5
Size: 47 4980 2081
Fragments arranged by size:
4980 2081 47
StuI AGG'CCT
Cuts at: 1517 2151 1517
Size: 634 6474
StyI C'CwwG_G
Cuts at: 513 1065 2565 3351 3742 4547 513
Size: 552 1500 786 391 805 3074
Fragments arranged by size:
3074 1500 805 786 552 391
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2137 3017 3642 3986 5135 5152 5305
Size: 1255 557 880 625 344 1149 17 153
Cuts at: 5305 5490 5935 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1149 880 625 557 445 344
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2373 3134 4208
Size: 274 80 33 51 1443 396 761 1074
Cuts at: 4208 5354 7066 96
Size: 1146 1712 138
Fragments arranged by size:
1712 1443 1146 1074 761 396 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2266 2298 2361 2393 3540 3572 3974 4006 4351
Size: 32 63 32 1147 32 402 32 345
Cuts at: 4351 4383 7068 7100 2266
Size: 32 2685 32 2274
Fragments arranged by size:
2685 2274 1147 402 345 63 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7108
XbaI T'CTAG_A
Cuts at: 4444 4444
Size: 7108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2681 2875 2681
Size: 194 6914
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7108
XmaI C'CCGG_G
Cuts at: 1870 4433 1870
Size: 2563 4545
XmnI GAAnn'nnTTC
Cuts at: 3300 5237 3300
Size: 1937 5171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4916 276
Size: 53 83 186 936 3382 2468
Fragments arranged by size:
3382 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BsiWI BsmBI
BstZ17I Bsu36I FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PspXI PsrI RsrII SacII SexAI
SfiI SfoI SrfI SwaI