Restriction map for
pFN21AB4977
(Circular) (Six-base) MAPSORT of: pFN21AB4977.seq Check: 4242 from: 1 to: 5905
pFN21AB4977 5905 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2024 3248 1937
Size: 87 1224 4594
Fragments arranged by size:
4594 1224 87
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3968 5352 5620 1632
Size: 2336 1384 268 1917
Fragments arranged by size:
2336 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 4804 5781 1203
Size: 3601 977 1327
Fragments arranged by size:
3601 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 2678 1166
Size: 1512 4393
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 2071 3648 5220 2071
Size: 1577 1572 2756
Fragments arranged by size:
2756 1577 1572
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 2594 3214 3440 3592 5701 1493
Size: 1101 620 226 152 2109 1697
Fragments arranged by size:
2109 1697 1101 620 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 3230 1870
Size: 73 1287 4545
Fragments arranged by size:
4545 1287 73
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3226 4681 618
Size: 325 217 627 1439 1455 1842
Fragments arranged by size:
1842 1455 1439 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3224 1391
Size: 1833 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4934
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4096 4130 1903
Size: 34 2159 34 3678
Fragments arranged by size:
3678 2159 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3797 5007 3797
Size: 1210 4695
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4516 136
Size: 107 122 71 631 605 2844 1525
Fragments arranged by size:
2844 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 2072 3322 2072
Size: 1250 4655
Bme1580I G_kGCm'C
Cuts at: 1790 2709 3966 5122 1790
Size: 919 1257 1156 2573
Fragments arranged by size:
2573 1257 1156 919
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3033 3636 4594
Size: 416 53 83 399 24 1616 603 958
Cuts at: 4594 442
Size: 1753
Fragments arranged by size:
1753 1616 958 603 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2502 2502
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 4565 1239
Size: 888 2438 2579
Fragments arranged by size:
2579 2438 888
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2252 4030 4895 5193 2252
Size: 1778 865 298 2964
Fragments arranged by size:
2964 1778 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4568 882
Size: 617 3069 2219
Fragments arranged by size:
3069 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2419 3696 493
Size: 711 1215 1277 2702
Fragments arranged by size:
2702 1277 1215 711
BsaBI GATnn'nnATC
Cuts at: 2613 2736 2844 5899 2613
Size: 123 108 3055 2619
Fragments arranged by size:
3055 2619 123 108
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3712 4094 4729 275
Size: 53 83 186 936 2179 382 635 1451
Fragments arranged by size:
2179 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2400 4338 4736 5010 5157 5568 2400
Size: 1938 398 274 147 411 2737
Fragments arranged by size:
2737 1938 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5680
Fragments arranged by size:
5680 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5905
BseYI C'CCAG_C
Cuts at: 5108 5604 5108
Size: 496 5409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5332
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3355 4116 5144 1540
Size: 275 186 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3966 4051 5122 3966
Size: 85 1071 4749
Fragments arranged by size:
4749 1071 85
BsmI GAATG_Cn'
Cuts at: 2824 3416 3509 2824
Size: 592 93 5220
Fragments arranged by size:
5220 592 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2103 2103
Size: 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2709 3224 3966 4051 5122 1391
Size: 399 919 515 742 85 1071 2174
Fragments arranged by size:
2174 1071 919 742 515 399 85
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3260 844
Size: 2416 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3792 1394
Size: 429 1969 3507
Fragments arranged by size:
3507 1969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4400 4574 65
Size: 1183 3152 174 1396
Fragments arranged by size:
3152 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2032 4549 4736 1700
Size: 110 222 2517 187 2869
Fragments arranged by size:
2869 2517 222 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3130 794
Size: 2336 3569
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2329 2504 2608 3187 3235 3602 3987 4004
Size: 357 175 104 579 48 367 385 17
Cuts at: 4004 5456 5542 5554 5900 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 579 385 367 357 346 175
104 86 48 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2569 513
Size: 552 1504 3849
Fragments arranged by size:
3849 1504 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2956 487
Size: 683 56 1730 3436
Fragments arranged by size:
3436 1730 683 56
BtsI GCAGTG_nn'
Cuts at: 2083 2552 3421 4215 4235 2083
Size: 469 869 794 20 3753
Fragments arranged by size:
3753 869 794 469 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2566 3352
Size: 54 1425 50 275 18 225 511 786
Cuts at: 3352 4241 5870 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 786 511 275 225 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3835 1353
Size: 2482 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4492 4866 5012 387
Size: 1064 138 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 3968 4565 5352 5620 1239
Size: 393 495 1841 597 787 268 1524
Fragments arranged by size:
1841 1524 787 597 495 393 268
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 5052 1678
Size: 3374 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2515 2547 2659 2691 4546
Size: 32 135 32 943 32 112 32 1855
Cuts at: 4546 4578 4620 4652 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1855 943 135 112 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2025 3249 3495 669
Size: 988 281 87 1224 246 3079
Fragments arranged by size:
3079 1224 988 281 246 87
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5019 5203 1305
Size: 3714 184 2007
Fragments arranged by size:
3714 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2568 10
Size: 54 1425 343 736 3347
Fragments arranged by size:
3347 1425 736 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2574 2678 2712 3863 4222 4381 518
Size: 648 1408 104 34 1151 359 159 2042
Fragments arranged by size:
2042 1408 1151 648 359 159 104 34
MspA1I CmG'CkG
Cuts at: 1606 3321 3998 5146 5391 1606
Size: 1715 677 1148 245 2120
Fragments arranged by size:
2120 1715 1148 677 245
NaeI GCC'GGC
Cuts at: 2034 2034
Size: 5905
NcoI C'CATG_G
Cuts at: 513 1065 2569 513
Size: 552 1504 3849
Fragments arranged by size:
3849 1504 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NgoMIV G'CCGG_C
Cuts at: 2032 2032
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 2050 3263 4808 1132
Size: 97 821 1213 1545 2229
Fragments arranged by size:
2229 1545 1213 821 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2294 1108
Size: 1186 4719
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2703 2735 3974 4006 5487 5519 2703
Size: 32 1239 32 1481 32 3089
Fragments arranged by size:
3089 1481 1239 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 2844 3475 2844
Size: 631 5274
PstI C_TGCA'G
Cuts at: 830 3257 830
Size: 2427 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 2023 3247 1936
Size: 87 1224 4594
Fragments arranged by size:
4594 1224 87
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 2349 2496 3253 4388 5069 5260 826
Size: 219 1304 147 757 1135 681 191 1471
Fragments arranged by size:
1471 1304 1135 757 681 219 191 147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 2032 1700
Size: 332 5573
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2267 4009 4910 5172 820
Size: 197 926 324 1742 901 262 1553
Fragments arranged by size:
1742 1553 926 901 324 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 2243 2688 3829 5
Size: 47 2191 445 1141 2081
Fragments arranged by size:
2191 2081 1141 445 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 2569 3201 3344 513
Size: 552 1504 632 143 3074
Fragments arranged by size:
3074 1504 632 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2735 3932 3949 4102 4287 4732 325
Size: 1255 1155 1197 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1197 1155 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4151 5863 96
Size: 274 80 33 51 1443 2174 1712 138
Fragments arranged by size:
2174 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5865 5897 5865
Size: 32 5873
Tth111I GACn'n_nGTC
Cuts at: 1316 2126 1316
Size: 810 5095
XbaI T'CTAG_A
Cuts at: 2507 3241 2507
Size: 734 5171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2291 2291
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 4034 4034
Size: 5905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI