Restriction map for
pFN21AB4976
(Circular) (Six-base) MAPSORT of: pFN21AB4976.seq Check: 5271 from: 1 to: 5959
pFN21AB4976 5959 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2064 2817 3302 1937
Size: 127 753 485 4594
Fragments arranged by size:
4594 753 485 127
Acc65I G'GTAC_C
Cuts at: 3230 3280 3230
Size: 50 5909
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4022 5406 5674 1632
Size: 2390 1384 268 1917
Fragments arranged by size:
2390 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2743 1184
Size: 1559 4400
AlwNI CAG_nnn'CTG
Cuts at: 3190 3702 5274 3190
Size: 512 1572 3875
Fragments arranged by size:
3875 1572 512
ApaI G_GGCC'C
Cuts at: 2403 2403
Size: 5959
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2461 2488 3268 3494 3646 5755 1493
Size: 968 27 780 226 152 2109 1697
Fragments arranged by size:
2109 1697 968 780 226 152 27
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 2276 2994 3284 1870
Size: 73 79 254 718 290 4545
Fragments arranged by size:
4545 718 290 254 79 73
BamHI G'GATC_C
Cuts at: 2134 3289 3656 2134
Size: 1155 367 4437
Fragments arranged by size:
4437 1155 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2791 2930 3230 3280 4735
Size: 325 217 627 1004 139 300 50 1455
Cuts at: 4735 618
Size: 1842
Fragments arranged by size:
1842 1455 1004 627 325 300 217 139
50
BanII G_rGCy'C
Cuts at: 1391 2403 3278 1391
Size: 1012 875 4072
Fragments arranged by size:
4072 1012 875
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2412 2898 928
Size: 971 513 486 3989
Fragments arranged by size:
3989 971 513 486
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2781 2815 4150 4184 1903
Size: 34 844 34 1335 34 3678
Fragments arranged by size:
3678 1335 844 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2622 2700 3013 5954 2622
Size: 78 313 2941 2627
Fragments arranged by size:
2941 2627 313 78
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 2403 2759 4020 5176 1790
Size: 613 356 1261 1156 2573
Fragments arranged by size:
2573 1261 1156 613 356
BmgBI CAC'GTC
Cuts at: 1318 2662 3031 1318
Size: 1344 369 4246
Fragments arranged by size:
4246 1344 369
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3690 4648 442
Size: 416 53 83 399 24 2273 958 1753
Fragments arranged by size:
2273 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4619 1239
Size: 3380 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2867 4084 4949 5247 2867
Size: 1217 865 298 3579
Fragments arranged by size:
3579 1217 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2552 4622 882
Size: 617 1053 2070 2219
Fragments arranged by size:
2219 2070 1053 617
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 2699 3126 5953 2699
Size: 427 2827 2705
Fragments arranged by size:
2827 2705 427
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3113 3766 4148 4783
Size: 53 83 186 936 1580 653 382 635
Cuts at: 4783 275
Size: 1451
Fragments arranged by size:
1580 1451 936 653 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2508 4392 4790 5064 5211 5622 2508
Size: 1884 398 274 147 411 2845
Fragments arranged by size:
2845 1884 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3223 3253 1178
Size: 30 165 30 1820 30 3884
Fragments arranged by size:
3884 1820 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2483 3236 1186
Size: 1297 753 3909
Fragments arranged by size:
3909 1297 753
BseYI C'CCAG_C
Cuts at: 2326 2544 2795 5162 5658 2326
Size: 218 251 2367 496 2627
Fragments arranged by size:
2627 2367 496 251 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5386
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 4020 4105 5176 2337
Size: 1683 85 1071 3120
Fragments arranged by size:
3120 1683 1071 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
BsmBI CGTCTCn'nnnn_
Cuts at: 3104 3104
Size: 5959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 2403 2759 3278 4020 4105 5176
Size: 399 547 66 356 519 742 85 1071
Cuts at: 5176 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 547 519 399 356 85
66
BspEI T'CCGG_A
Cuts at: 2508 5622 2508
Size: 3114 2845
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2186 3314 844
Size: 1342 1128 3489
Fragments arranged by size:
3489 1342 1128
BsrBI CCG'CTC
Cuts at: 1394 1823 2155 3846 1394
Size: 429 332 1691 3507
Fragments arranged by size:
3507 1691 429 332
BsrDI GCAATG_nn'
Cuts at: 65 1248 4454 4628 65
Size: 1183 3206 174 1396
Fragments arranged by size:
3206 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2269 3233 4603 4790 1700
Size: 110 459 964 1370 187 2869
Fragments arranged by size:
2869 1370 964 459 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 2102 4019 5031 2102
Size: 1917 1012 3030
Fragments arranged by size:
3030 1917 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstXI CCAn_nnnn'nTGG
Cuts at: 3031 3031
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2134 2358 2475 2622 2700 2943 3013 3289
Size: 162 224 117 147 78 243 70 276
Cuts at: 3289 3656 4041 4058 5510 5596 5608 5954 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 276 243 224
162 147 117 86 78 70 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2632 2766 3036 513
Size: 552 939 628 134 270 3436
Fragments arranged by size:
3436 939 628 552 270 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 2387 3475 4269 4289 2387
Size: 1088 794 20 4057
Fragments arranged by size:
4057 1088 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2210 2438 4966 809
Size: 507 894 228 2528 1802
Fragments arranged by size:
2528 1802 894 507 228
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2194 2247 3033
Size: 54 1425 50 275 18 364 53 786
Cuts at: 3033 3406 4295 5924 8
Size: 373 889 1629 43
Fragments arranged by size:
1629 1425 889 786 373 364 275 54
53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2766 3213 3889 1353
Size: 1413 447 676 3423
Fragments arranged by size:
3423 1413 676 447
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4022 4619 5406 5674 1239
Size: 393 2390 597 787 268 1524
Fragments arranged by size:
2390 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2019 2203 2236 2399 2860 2019
Size: 184 33 163 461 5118
Fragments arranged by size:
5118 461 184 163 33
EcoRI G'AATT_C
Cuts at: 2488 3268 3646 2488
Size: 780 378 4801
Fragments arranged by size:
4801 780 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 5959
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 5106 1678
Size: 3428 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2628 2660 3005 3037 4600
Size: 32 135 32 1056 32 345 32 1563
Cuts at: 4600 4632 4674 4706 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1563 1056 345 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2653 3303 3549 669
Size: 988 281 715 650 246 3079
Fragments arranged by size:
3079 988 715 650 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3234 3284 3234
Size: 50 5909
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2562 5073 5257 1305
Size: 1257 2511 184 2007
Fragments arranged by size:
2511 2007 1257 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2249 10
Size: 54 1425 343 417 3720
Fragments arranged by size:
3720 1425 417 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3056 3917 4276 4435 518
Size: 648 1890 861 359 159 2042
Fragments arranged by size:
2042 1890 861 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3375 4052 5200 5445 1606
Size: 1769 677 1148 245 2120
Fragments arranged by size:
2120 1769 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2632 2766 513
Size: 552 939 628 134 3706
Fragments arranged by size:
3706 939 628 552 134
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3317 4862 1132
Size: 97 2088 1545 2229
Fragments arranged by size:
2229 2088 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 4028 4060 5541 5573 2891
Size: 32 1105 32 1481 32 3277
Fragments arranged by size:
3277 1481 1105 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2019 2203 2236 2019
Size: 184 33 5742
Fragments arranged by size:
5742 184 33
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PspOMI G'GGCC_C
Cuts at: 2399 2399
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2307 2394 3311 830
Size: 1477 87 917 3478
Fragments arranged by size:
3478 1477 917 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SanDI GG'GwC_CC
Cuts at: 2019 2019
Size: 5959
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2303 2390 3307 4442 5123 5314 826
Size: 219 1258 87 917 1135 681 191 1471
Fragments arranged by size:
1471 1258 1135 917 681 219 191 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2882 4063 4964 5226 820
Size: 197 926 939 1181 901 262 1553
Fragments arranged by size:
1553 1181 939 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 2536 1517
Size: 1019 4940
StyI C'CwwG_G
Cuts at: 513 1065 2004 2632 2766 2868 3398 513
Size: 552 939 628 134 102 530 3074
Fragments arranged by size:
3074 939 628 552 530 134 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2809 3986 4003 4156 4341 4786 325
Size: 1255 1229 1177 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1229 1177 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2748 4205 5917
Size: 274 80 33 51 1443 771 1457 1712
Cuts at: 5917 96
Size: 138
Fragments arranged by size:
1712 1457 1443 771 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5919 5951 5919
Size: 32 5927
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI