Restriction map for
pFN21AB4969
(Circular) (Six-base) MAPSORT of: pFN21AB4969.seq Check: 5814 from: 1 to: 11377
pFN21AB4969 11377 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 7500 7500
Size: 11377
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9187 278
Size: 53 83 186 936 7651 2468
Fragments arranged by size:
7651 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2199 4549 5059 8720 1937
Size: 262 2350 510 3661 4594
Fragments arranged by size:
4594 3661 2350 510 262
Acc65I G'GTAC_C
Cuts at: 3675 6714 8698 3675
Size: 3039 1984 6354
Fragments arranged by size:
6354 3039 1984
AclI AA'CG_TT
Cuts at: 1563 9504 9877 1563
Size: 7941 373 3063
Fragments arranged by size:
7941 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2301 4170 4521 5084 5412 5854 5989 6783
Size: 669 1869 351 563 328 442 135 794
Cuts at: 6783 6975 7764 7979 9440 10824 11092 1632
Size: 192 789 215 1461 1384 268 1917
Fragments arranged by size:
1917 1869 1461 1384 794 789 669 563
442 351 328 268 215 192 135
AflII C'TTAA_G
Cuts at: 820 1017 3011 5195 820
Size: 197 1994 2184 7002
Fragments arranged by size:
7002 2184 1994 197
AflIII A'CryG_T
Cuts at: 1203 8326 8563 10276 11253 1203
Size: 7123 237 1713 977 1327
Fragments arranged by size:
7123 1713 1327 977 237
AgeI A'CCGG_T
Cuts at: 10208 10208
Size: 11377
AhdI GACnn_n'nnGTC
Cuts at: 10106 10106
Size: 11377
AleI CACnn'nnGTG
Cuts at: 1166 8475 1166
Size: 7309 4068
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 11377
AlwNI CAG_nnn'CTG
Cuts at: 2830 7722 9120 10692 2830
Size: 4892 1398 1572 3515
Fragments arranged by size:
4892 3515 1572 1398
ApaI G_GGCC'C
Cuts at: 2571 2909 2571
Size: 338 11039
ApaLI G'TGCA_C
Cuts at: 9434 10590 9434
Size: 1156 10221
ApoI r'AATT_y
Cuts at: 1493 3864 4003 4037 4292 4571 5311 6201 6434
Size: 2371 139 34 255 279 740 890 233
Cuts at: 6434 6521 6788 6930 6988 7307 7871 8686 8912
Size: 87 267 142 58 319 564 815 226
Cuts at: 8912 9064 11173 1493
Size: 152 2109 1697
Fragments arranged by size:
2371 2109 1697 890 815 740 564 319
279 267 255 233 226 152 142 139
87 58 34
AseI AT'TA_AT
Cuts at: 160 1032 7428 9931 160
Size: 872 6396 2503 1606
Fragments arranged by size:
6396 2503 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11377
AvaI C'yCGr_G
Cuts at: 1870 1943 2554 2788 6903 8142 8702 1870
Size: 73 611 234 4115 1239 560 4545
Fragments arranged by size:
4545 4115 1239 611 560 234 73
BamHI G'GATC_C
Cuts at: 4516 7890 7929 8707 9074 4516
Size: 3374 39 778 367 6819
Fragments arranged by size:
6819 3374 778 367 39
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 3675 6714 7943 8698
Size: 325 217 627 261 1627 3039 1229 755
Cuts at: 8698 10153 618
Size: 1455 1842
Fragments arranged by size:
3039 1842 1627 1455 1229 755 627 325
261 217
BanII G_rGCy'C
Cuts at: 1391 2151 2571 2789 2909 3280 5463 7806 8696
Size: 760 420 218 120 371 2183 2343 890
Cuts at: 8696 1391
Size: 4072
Fragments arranged by size:
4072 2343 2183 890 760 420 371 218
120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2624 4080 4509 4902 5197 6822 928
Size: 971 725 1456 429 393 295 1625 5483
Fragments arranged by size:
5483 1625 1456 971 725 429 393 295
BbvCI CC'TCA_GC
Cuts at: 4028 5573 5627 5708 5735 5762 5843 5978 6113
Size: 1545 54 81 27 27 81 135 135
Cuts at: 6113 6302 4028
Size: 189 9103
Fragments arranged by size:
9103 1545 189 135 135 81 81 54
27 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4276 4310 9568 9602 1903
Size: 34 2339 34 5258 34 3678
Fragments arranged by size:
5258 3678 2339 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4067 4536 9269 10479 4067
Size: 469 4733 1210 4965
Fragments arranged by size:
4965 4733 1210 469
BclI T'GATC_A
Cuts at: 1548 3417 4920 5410 6385 1548
Size: 1869 1503 490 975 6540
Fragments arranged by size:
6540 1869 1503 975 490
BfrBI ATG'CAT
Cuts at: 4132 4132
Size: 11377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7181 9988 136
Size: 107 122 71 631 605 5509 2807 1525
Fragments arranged by size:
5509 2807 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6190 6259 8019 11372 6190
Size: 69 1760 3353 6195
Fragments arranged by size:
6195 3353 1760 69
BlpI GC'TnA_GC
Cuts at: 4593 8794 4593
Size: 4201 7176
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2571 2752 2909 3891 5370 6639 8308
Size: 261 520 181 157 982 1479 1269 1669
Cuts at: 8308 9438 10594 1790
Size: 1130 1156 2573
Fragments arranged by size:
2573 1669 1479 1269 1156 1130 982 520
261 181 157
BmgBI CAC'GTC
Cuts at: 1318 3529 8327 1318
Size: 2211 4798 4368
Fragments arranged by size:
4798 4368 2211
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3493 3894 5430
Size: 416 53 83 399 24 2076 401 1536
Cuts at: 5430 7807 8299 9108 10066 442
Size: 2377 492 809 958 1753
Fragments arranged by size:
2377 2076 1753 1536 958 809 492 416
401 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3311 3311
Size: 11377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2418 2695 2852 4083 4298 4587 5331 5439
Size: 1179 277 157 1231 215 289 744 108
Cuts at: 5439 5550 5577 5604 5631 5685 5712 5766 5793
Size: 111 27 27 27 54 27 54 27
Cuts at: 5793 5820 5874 5901 5955 6009 6063 6117 6225
Size: 27 54 27 54 54 54 54 108
Cuts at: 6225 6306 6360 6670 10037 1239
Size: 81 54 310 3367 2579
Fragments arranged by size:
3367 2579 1231 1179 744 310 289 277
215 157 111 108 108 81 54 54
54 54 54 54 54 54 27 27
27 27 27 27 27
Bpu10I CC'TnA_GC
Cuts at: 2096 2118 2145 4028 4685 5573 5627 5708 5735
Size: 22 27 1883 657 888 54 81 27
Cuts at: 5735 5762 5843 5978 6113 6302 2096
Size: 27 81 135 135 189 7171
Fragments arranged by size:
7171 1883 888 657 189 135 135 81
81 54 27 27 27 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2143 2331 2353 3701 5495 8372 9502 10367 10665
Size: 188 22 1348 1794 2877 1130 865 298
Cuts at: 10665 2143
Size: 2855
Fragments arranged by size:
2877 2855 1794 1348 1130 865 298 188
22
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2857 3138 10040 882
Size: 617 1358 281 6902 2219
Fragments arranged by size:
6902 2219 1358 617 281
BsaAI yAC'GTr
Cuts at: 493 1204 2110 4943 7129 7525 9168 493
Size: 711 906 2833 2186 396 1643 2702
Fragments arranged by size:
2833 2702 2186 1643 906 711 396
BsaBI GATnn'nnATC
Cuts at: 11371 11371
Size: 11377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 9184 9566 10201 275
Size: 53 83 186 936 7651 382 635 1451
Fragments arranged by size:
7651 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4709 9810 10208 10482 10629 11040 4709
Size: 5101 398 274 147 411 5046
Fragments arranged by size:
5101 5046 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2452 2482 3939 3969 4628
Size: 30 165 30 1049 30 1457 30 659
Cuts at: 4628 4658 4687 4717 4738 4768 5575 5605 5629
Size: 30 29 30 21 30 807 30 24
Cuts at: 5629 5659 5710 5740 5899 5929 6223 6253 6304
Size: 30 51 30 159 30 294 30 51
Cuts at: 6304 6334 6665 6695 1178
Size: 30 331 30 5860
Fragments arranged by size:
5860 1457 1049 807 659 331 294 165
159 51 51 30 30 30 30 30
30 30 30 30 30 30 30 30
30 29 24 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 6363 6673 1186
Size: 5177 310 5890
Fragments arranged by size:
5890 5177 310
BseYI C'CCAG_C
Cuts at: 2055 2064 2776 2957 5674 5944 6052 6086 10580
Size: 9 712 181 2717 270 108 34 4494
Cuts at: 10580 11076 2055
Size: 496 2356
Fragments arranged by size:
4494 2717 2356 712 496 270 181 108
34 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 10804
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8827 9588 10616 1540
Size: 275 186 6826 761 1028 2301
Fragments arranged by size:
6826 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2789 3767 5463 9438 9523 10594 2789
Size: 978 1696 3975 85 1071 3572
Fragments arranged by size:
3975 3572 1696 1071 978 85
BsmI GAATG_Cn'
Cuts at: 4483 4939 5200 8320 8338 8888 8981 4483
Size: 456 261 3120 18 550 93 6879
Fragments arranged by size:
6879 3120 550 456 261 93 18
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2151 2571 2752 2789 2909 3280
Size: 399 261 100 420 181 37 120 371
Cuts at: 3280 3767 3891 5370 5463 6639 7806 8308 8696
Size: 487 124 1479 93 1176 1167 502 388
Cuts at: 8696 9438 9523 10594 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1479 1176 1167 1071 742 502 487
420 399 388 371 261 181 124 120
100 93 85 37
BspEI T'CCGG_A
Cuts at: 4709 11040 4709
Size: 6331 5046
BspHI T'CATG_A
Cuts at: 3537 3759 9266 10996 3537
Size: 222 5507 1730 3918
Fragments arranged by size:
5507 3918 1730 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 7500 8732 844
Size: 6656 1232 3489
Fragments arranged by size:
6656 3489 1232
BsrBI CCG'CTC
Cuts at: 1394 1823 9264 1394
Size: 429 7441 3507
Fragments arranged by size:
7441 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3063 5258 6419 6702 6753 9872 10046
Size: 1183 1815 2195 1161 283 51 3119 174
Cuts at: 10046 65
Size: 1396
Fragments arranged by size:
3119 2195 1815 1396 1183 1161 283 174
51
BsrFI r'CCGG_y
Cuts at: 1700 1810 10021 10208 1700
Size: 110 8211 187 2869
Fragments arranged by size:
8211 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2697 3189 6793 96
Size: 2601 492 3604 4680
Fragments arranged by size:
4680 3604 2601 492
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11377
BssSI C'ACGA_G
Cuts at: 3024 9437 10449 3024
Size: 6413 1012 3952
Fragments arranged by size:
6413 3952 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2638 7874 794
Size: 1844 5236 4297
Fragments arranged by size:
5236 4297 1844
BstBI TT'CG_AA
Cuts at: 6865 10181 6865
Size: 3316 8061
BstEII G'GTnAC_C
Cuts at: 1184 5581 1184
Size: 4397 6980
BstXI CCAn_nnnn'nTGG
Cuts at: 2028 3884 4697 4748 8212 2028
Size: 1856 813 51 3464 5193
Fragments arranged by size:
5193 3464 1856 813 51
BstYI r'GATC_y
Cuts at: 1972 3590 4516 5444 6190 6259 6352 6691 7832
Size: 1618 926 928 746 69 93 339 1141
Cuts at: 7832 7890 7929 8019 8130 8707 9074 9459 9476
Size: 58 39 90 111 577 367 385 17
Cuts at: 9476 10928 11014 11026 11372 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1618 1452 1141 928 926 746 577
385 367 346 339 111 93 90 86
69 58 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 3397 3397
Size: 11377
BtgI C'CryG_G
Cuts at: 513 1065 7021 8520 513
Size: 552 5956 1499 3370
Fragments arranged by size:
5956 3370 1499 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3532 487
Size: 683 56 2306 8332
Fragments arranged by size:
8332 2306 683 56
BtsI GCAGTG_nn'
Cuts at: 6610 8372 8893 9687 9707 6610
Size: 1762 521 794 20 8280
Fragments arranged by size:
8280 1762 794 521 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11342
DraI TTT'AAA
Cuts at: 2311 6985 7612 8681 2311
Size: 4674 627 1069 5007
Fragments arranged by size:
5007 4674 1069 627
DraIII CAC_nnn'GTG
Cuts at: 4562 4562
Size: 11377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10384 809
Size: 507 9068 1802
Fragments arranged by size:
9068 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2821 3318 4993
Size: 54 1425 50 275 18 991 497 1675
Cuts at: 4993 7540 8824 9713 11342 8
Size: 2547 1284 889 1629 43
Fragments arranged by size:
2547 1675 1629 1425 1284 991 889 497
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8824 1537
Size: 275 7012 4090
Fragments arranged by size:
7012 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3041 3391 3938 4152 4245 5241 5455 6585
Size: 1688 350 547 214 93 996 214 1130
Cuts at: 6585 6912 7337 9307 1353
Size: 327 425 1970 3423
Fragments arranged by size:
3423 1970 1688 1130 996 547 425 350
327 214 214 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9964 10338 10484 387
Size: 1064 138 8375 374 146 1280
Fragments arranged by size:
8375 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2787 5461 2787
Size: 2674 8703
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 2418 2695 2852 4083 4170 4298
Size: 393 669 117 277 157 1231 87 128
Cuts at: 4298 4521 4587 5084 5331 5412 5439 5550 5577
Size: 223 66 497 247 81 27 111 27
Cuts at: 5577 5604 5631 5685 5712 5766 5793 5820 5854
Size: 27 27 54 27 54 27 27 34
Cuts at: 5854 5874 5901 5955 5989 6009 6063 6117 6225
Size: 20 27 54 34 20 54 54 108
Cuts at: 6225 6306 6360 6670 6783 6975 7764 7979 9440
Size: 81 54 310 113 192 789 215 1461
Cuts at: 9440 10037 10824 11092 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1461 1231 789 787 669 597 497
393 310 277 268 247 223 215 192
157 128 117 113 111 108 87 81
81 66 54 54 54 54 54 54
34 34 27 27 27 27 27 27
27 27 20 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2600 3400 5576 6305 2600
Size: 800 2176 729 7672
Fragments arranged by size:
7672 2176 800 729
EcoO109I rG'GnC_Cy
Cuts at: 2032 2568 2592 2827 3263 3852 4604 4719 4729
Size: 536 24 235 436 589 752 115 10
Cuts at: 4729 4770 4780 5142 7241 2032
Size: 41 10 362 2099 6168
Fragments arranged by size:
6168 2099 752 589 536 436 362 235
115 41 24 10 10
EcoRI G'AATT_C
Cuts at: 4003 4292 4571 5311 6930 8686 9064 4003
Size: 289 279 740 1619 1756 378 6316
Fragments arranged by size:
6316 1756 1619 740 378 289 279
EcoRV GAT'ATC
Cuts at: 6366 6366
Size: 11377
FspI TGC'GCA
Cuts at: 9883 9883
Size: 11377
HaeII r_GCGC'y
Cuts at: 1678 10524 1678
Size: 8846 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2326 2358 2537 2569 3137
Size: 32 135 32 754 32 179 32 568
Cuts at: 3137 3169 3247 3267 3279 3299 3536 3568 4061
Size: 32 78 20 12 20 237 32 493
Cuts at: 4061 4093 5575 5607 5629 5661 5899 5931 6223
Size: 32 1482 32 22 32 238 32 292
Cuts at: 6223 6255 6304 6336 6355 6387 6463 6495 7211
Size: 32 49 32 19 32 76 32 716
Cuts at: 7211 7243 7296 7328 10018 10050 10092 10124 1373
Size: 32 53 32 2690 32 42 32 2626
Fragments arranged by size:
2690 2626 1482 754 716 568 493 292
238 237 179 135 78 76 53 49
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 22 20 20 19 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3382 3993 4909 8721 8967 669
Size: 988 281 1444 611 916 3812 246 3079
Fragments arranged by size:
3812 3079 1444 988 916 611 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 11377
HpaI GTT'AAC
Cuts at: 3993 4909 8967 3993
Size: 916 4058 6403
Fragments arranged by size:
6403 4058 916
KpnI G_GTAC'C
Cuts at: 3679 6718 8702 3679
Size: 3039 1984 6354
Fragments arranged by size:
6354 3039 1984
MfeI C'AATT_G
Cuts at: 4912 8976 4912
Size: 4064 7313
MluI A'CGCG_T
Cuts at: 11253 11253
Size: 11377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2111 2266 3958 7648 8025 8162 10491 10675
Size: 806 155 1692 3690 377 137 2329 184
Cuts at: 10675 1305
Size: 2007
Fragments arranged by size:
3690 2329 2007 1692 806 377 184 155
137
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3320 4995 10
Size: 54 1425 343 1488 1675 6392
Fragments arranged by size:
6392 1675 1488 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 7020 7292 7523 8475 9335 9694 9853
Size: 648 5854 272 231 952 860 359 159
Cuts at: 9853 518
Size: 2042
Fragments arranged by size:
5854 2042 952 860 648 359 272 231
159
MspA1I CmG'CkG
Cuts at: 1606 2055 2766 4421 4904 7202 8793 9470 10618
Size: 449 711 1655 483 2298 1591 677 1148
Cuts at: 10618 10863 1606
Size: 245 2120
Fragments arranged by size:
2298 2120 1655 1591 1148 711 677 483
449 245
NcoI C'CATG_G
Cuts at: 513 1065 7021 8520 513
Size: 552 5956 1499 3370
Fragments arranged by size:
5956 3370 1499 552
NdeI CA'TA_TG
Cuts at: 387 4081 5904 387
Size: 3694 1823 5860
Fragments arranged by size:
5860 3694 1823
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11377
NotI GC'GGCC_GC
Cuts at: 8824 8824
Size: 11377
NsiI A_TGCA'T
Cuts at: 4134 4134
Size: 11377
NspI r_CATG'y
Cuts at: 1132 1229 4592 7019 7139 7291 7906 8567 8735
Size: 97 3363 2427 120 152 615 661 168
Cuts at: 8735 10280 1132
Size: 1545 2229
Fragments arranged by size:
3363 2427 2229 1545 661 615 168 152
120 97
PasI CC'CwG_GG
Cuts at: 1348 4722 8212 8320 1348
Size: 3374 3490 108 4405
Fragments arranged by size:
4405 3490 3374 108
PciI A'CATG_T
Cuts at: 8563 10276 8563
Size: 1713 9664
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2830 6248 6275 6464 1108
Size: 1722 3418 27 189 6021
Fragments arranged by size:
6021 3418 1722 189 27
PfoI T'CCnGG_A
Cuts at: 2829 4646 6348 2829
Size: 1817 1702 7858
Fragments arranged by size:
7858 1817 1702
PmeI GTTT'AAAC
Cuts at: 8681 8681
Size: 11377
PmlI CAC'GTG
Cuts at: 4943 7525 4943
Size: 2582 8795
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9446 9478 10959 10991 9446
Size: 32 1481 32 9832
Fragments arranged by size:
9832 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2032 2592 2827 3263 3852 4604 4729 4780 5142
Size: 560 235 436 589 752 125 51 362
Cuts at: 5142 7241 2032
Size: 2099 6168
Fragments arranged by size:
6168 2099 752 589 560 436 362 235
125 51
PshAI GACnn'nnGTC
Cuts at: 1613 5328 5436 5487 6465 1613
Size: 3715 108 51 978 6525
Fragments arranged by size:
6525 3715 978 108 51
PsiI TTA'TAA
Cuts at: 3177 3453 7839 8414 8947 3177
Size: 276 4386 575 533 5607
Fragments arranged by size:
5607 4386 575 533 276
PspOMI G'GGCC_C
Cuts at: 2567 2905 2567
Size: 338 11039
PspXI vC'TCGA_Gb
Cuts at: 6903 8142 6903
Size: 1239 10138
PstI C_TGCA'G
Cuts at: 830 3073 4201 8729 830
Size: 2243 1128 4528 3478
Fragments arranged by size:
4528 3478 2243 1128
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11377
PvuII CAG'CTG
Cuts at: 2055 2766 4421 4904 7202 2055
Size: 711 1655 483 2298 6230
Fragments arranged by size:
6230 2298 1655 711 483
SacI G_AGCT'C
Cuts at: 2789 5463 2789
Size: 2674 8703
SalI G'TCGA_C
Cuts at: 1936 8719 1936
Size: 6783 4594
SanDI GG'GwC_CC
Cuts at: 2592 3852 4604 2592
Size: 1260 752 9365
Fragments arranged by size:
9365 1260 752
SapI GCTCTTCn'nnn_
Cuts at: 6912 7337 6912
Size: 425 10952
SbfI CC_TGCA'GG
Cuts at: 8729 8729
Size: 11377
ScaI AGT'ACT
Cuts at: 9625 11337 9625
Size: 1712 9665
SfcI C'TryA_G
Cuts at: 826 1045 2200 3069 3331 3520 3841 4107 4197
Size: 219 1155 869 262 189 321 266 90
Cuts at: 4197 4509 4523 7441 7723 8473 8725 9860 10541
Size: 312 14 2918 282 750 252 1135 681
Cuts at: 10541 10732 826
Size: 191 1471
Fragments arranged by size:
2918 1471 1155 1135 869 750 681 321
312 282 266 262 252 219 191 189
90 14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11377
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11377
SmaI CCC'GGG
Cuts at: 1872 8704 1872
Size: 6832 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2158 2332 2346 3011 3680 5195
Size: 197 926 215 174 14 665 669 1515
Cuts at: 5195 5474 6903 8142 8387 9481 10382 10644 820
Size: 279 1429 1239 245 1094 901 262 1553
Fragments arranged by size:
1553 1515 1429 1239 1094 926 901 669
665 279 262 245 215 197 174 14
SnaBI TAC'GTA
Cuts at: 493 7129 9168 493
Size: 6636 2039 2702
Fragments arranged by size:
6636 2702 2039
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11377
SphI G_CATG'C
Cuts at: 1132 7139 8735 1132
Size: 6007 1596 3774
Fragments arranged by size:
6007 3774 1596
SspI AAT'ATT
Cuts at: 5 52 4351 9301 5
Size: 47 4299 4950 2081
Fragments arranged by size:
4950 4299 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 11377
StyI C'CwwG_G
Cuts at: 513 1065 2074 4608 6247 6463 7021 7886 7939
Size: 552 1009 2534 1639 216 558 865 53
Cuts at: 7939 8346 8520 8816 513
Size: 407 174 296 3074
Fragments arranged by size:
3074 2534 1639 1009 865 558 552 407
296 216 174 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2501 5013 5499 5672 5807 5942 6050
Size: 1255 921 2512 486 173 135 135 108
Cuts at: 6050 7468 9404 9421 9574 9759 10204 325
Size: 1418 1936 17 153 185 445 1498
Fragments arranged by size:
2512 1936 1498 1418 1255 921 486 445
185 173 153 135 135 108 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2697 3189 6793
Size: 274 80 33 51 1443 720 492 3604
Cuts at: 6793 9623 11335 96
Size: 2830 1712 138
Fragments arranged by size:
3604 2830 1712 1443 720 492 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2452 2484 3939 3971 4628 4660 4687 4719 4738
Size: 32 1455 32 657 32 27 32 19
Cuts at: 4738 4770 11337 11369 2452
Size: 32 6567 32 2460
Fragments arranged by size:
6567 2460 1455 657 32 32 32 32
32 32 27 19
Tth111I GACn'n_nGTC
Cuts at: 1316 3824 1316
Size: 2508 8869
XbaI T'CTAG_A
Cuts at: 5863 5998 6106 8713 5863
Size: 135 108 2607 8527
Fragments arranged by size:
8527 2607 135 108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2916 2916
Size: 11377
XhoI C'TCGA_G
Cuts at: 1943 6903 8142 1943
Size: 4960 1239 5178
Fragments arranged by size:
5178 4960 1239
XmaI C'CCGG_G
Cuts at: 1870 8702 1870
Size: 6832 4545
XmnI GAAnn'nnTTC
Cuts at: 5655 5668 8576 9506 5655
Size: 13 2908 930 7526
Fragments arranged by size:
7526 2908 930 13
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9185 276
Size: 53 83 186 936 7651 2468
Fragments arranged by size:
7651 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PsrI RsrII SacII SexAI SfiI SfoI
SrfI SwaI