Restriction map for
pFN21AB4964
(Circular) (Six-base) MAPSORT of: pFN21AB4964.seq Check: 3810 from: 1 to: 9097
pFN21AB4964 9097 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2429 6907 278
Size: 53 83 186 936 893 4478 2468
Fragments arranged by size:
4478 2468 936 893 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2429 5802 5892 6440 1937
Size: 492 3373 90 548 4594
Fragments arranged by size:
4594 3373 548 492 90
Acc65I G'GTAC_C
Cuts at: 6418 6418
Size: 9097
AclI AA'CG_TT
Cuts at: 1563 3556 7224 7597 1563
Size: 1993 3668 373 3063
Fragments arranged by size:
3668 3063 1993 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2501 2681 3416 3582 3724 4283 4328 5750
Size: 869 180 735 166 142 559 45 1422
Cuts at: 5750 7160 8544 8812 1632
Size: 1410 1384 268 1917
Fragments arranged by size:
1917 1422 1410 1384 869 735 559 268
180 166 142 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8900
AflIII A'CryG_T
Cuts at: 1203 2259 3287 4683 6358 7996 8973 1203
Size: 1056 1028 1396 1675 1638 977 1327
Fragments arranged by size:
1675 1638 1396 1327 1056 1028 977
AgeI A'CCGG_T
Cuts at: 4280 7928 4280
Size: 3648 5449
AhdI GACnn_n'nnGTC
Cuts at: 2272 4843 7826 2272
Size: 2571 2983 3543
Fragments arranged by size:
3543 2983 2571
AleI CACnn'nnGTG
Cuts at: 1166 2258 4556 1166
Size: 1092 2298 5707
Fragments arranged by size:
5707 2298 1092
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9097
AlwNI CAG_nnn'CTG
Cuts at: 2341 5652 6840 8412 2341
Size: 3311 1188 1572 3026
Fragments arranged by size:
3311 3026 1572 1188
ApaI G_GGCC'C
Cuts at: 2174 2174
Size: 9097
ApaLI G'TGCA_C
Cuts at: 3074 4625 7154 8310 3074
Size: 1551 2529 1156 3861
Fragments arranged by size:
3861 2529 1551 1156
ApoI r'AATT_y
Cuts at: 1493 2549 3974 4124 4822 5971 6340 6406 6632
Size: 1056 1425 150 698 1149 369 66 226
Cuts at: 6632 6784 8893 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1425 1149 1056 698 369 226
152 150 66
AseI AT'TA_AT
Cuts at: 160 1032 7651 160
Size: 872 6619 1606
Fragments arranged by size:
6619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9097
AvaI C'yCGr_G
Cuts at: 1870 1943 2174 2945 5429 6013 6422 1870
Size: 73 231 771 2484 584 409 4545
Fragments arranged by size:
4545 2484 771 584 409 231 73
AvrII C'CTAG_G
Cuts at: 5150 5900 5150
Size: 750 8347
BamHI G'GATC_C
Cuts at: 2419 4670 5461 6427 6794 2419
Size: 2251 791 966 367 4722
Fragments arranged by size:
4722 2251 966 791 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2068 3629 6418 7873 618
Size: 325 217 627 281 1561 2789 1455 1842
Fragments arranged by size:
2789 1842 1561 1455 627 325 281 217
BanII G_rGCy'C
Cuts at: 1391 2174 2805 5150 6416 1391
Size: 783 631 2345 1266 4072
Fragments arranged by size:
4072 2345 1266 783 631
BbeI G_GCGC'C
Cuts at: 2072 2072
Size: 9097
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2503 3270 5760 928
Size: 971 604 767 2490 4265
Fragments arranged by size:
4265 2490 971 767 604
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3516 3550 7288 7322 1903
Size: 34 1579 34 3738 34 3678
Fragments arranged by size:
3738 3678 1579 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3746 6989 8199 3746
Size: 3243 1210 4644
Fragments arranged by size:
4644 3243 1210
BclI T'GATC_A
Cuts at: 1548 2403 2613 1548
Size: 855 210 8032
Fragments arranged by size:
8032 855 210
BfrBI ATG'CAT
Cuts at: 4108 4108
Size: 9097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3950 4487 7708
Size: 107 122 71 631 605 2278 537 3221
Cuts at: 7708 136
Size: 1525
Fragments arranged by size:
3221 2278 1525 631 605 537 122 107
71
BglII A'GATC_T
Cuts at: 5352 9092 5352
Size: 3740 5357
BlpI GC'TnA_GC
Cuts at: 3152 6514 3152
Size: 3362 5735
Bme1580I G_kGCm'C
Cuts at: 1790 2174 2284 3078 3585 4629 5821 7158 8314
Size: 384 110 794 507 1044 1192 1337 1156
Cuts at: 8314 1790
Size: 2573
Fragments arranged by size:
2573 1337 1192 1156 1044 794 507 384
110
BmgBI CAC'GTC
Cuts at: 1318 2140 2203 3288 1318
Size: 822 63 1085 7127
Fragments arranged by size:
7127 1085 822 63
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2863 4404 5398
Size: 416 53 83 399 24 1446 1541 994
Cuts at: 5398 5460 5824 6828 7786 442
Size: 62 364 1004 958 1753
Fragments arranged by size:
1753 1541 1446 1004 994 958 416 399
364 83 62 53 24
BmtI G_CTAG'C
Cuts at: 1056 3936 1056
Size: 2880 6217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 2950 4808 6087 2752
Size: 198 1858 1279 5762
Fragments arranged by size:
5762 1858 1279 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2888 2945 3257 4397 4635 5984 6068 7757
Size: 1649 57 312 1140 238 1349 84 1689
Cuts at: 7757 1239
Size: 2579
Fragments arranged by size:
2579 1689 1649 1349 1140 312 238 84
57
Bpu10I CC'TnA_GC
Cuts at: 4493 5855 5915 4493
Size: 1362 60 7675
Fragments arranged by size:
7675 1362 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4726 5927 5986 6238 7222 8087 8385 4726
Size: 1201 59 252 984 865 298 5438
Fragments arranged by size:
5438 1201 984 865 298 252 59
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2791 4012 4373 5603 7760 882
Size: 617 1292 1221 361 1230 2157 2219
Fragments arranged by size:
2219 2157 1292 1230 1221 617 361
BsaAI yAC'GTr
Cuts at: 493 1204 2260 5211 6888 493
Size: 711 1056 2951 1677 2702
Fragments arranged by size:
2951 2702 1677 1056 711
BsaBI GATnn'nnATC
Cuts at: 9091 9091
Size: 9097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2069 2426 4149 6904
Size: 53 83 186 936 536 357 1723 2755
Cuts at: 6904 7286 7921 275
Size: 382 635 1451
Fragments arranged by size:
2755 1723 1451 936 635 536 382 357
186 83 53
BsaWI w'CCGG_w
Cuts at: 4280 4698 5508 7530 7928 8202 8349 8760 4280
Size: 418 810 2022 398 274 147 411 4617
Fragments arranged by size:
4617 2022 810 418 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4370 4400 4721 4751 6074
Size: 30 165 30 2967 30 321 30 1323
Cuts at: 6074 6104 1178
Size: 30 4171
Fragments arranged by size:
4171 2967 1323 321 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2344 3254 4385 4685 4729 6076 1186
Size: 1158 910 1131 300 44 1347 4207
Fragments arranged by size:
4207 1347 1158 1131 910 300 44
BseYI C'CCAG_C
Cuts at: 2122 2595 2777 4220 5782 8300 8796 2122
Size: 473 182 1443 1562 2518 496 2423
Fragments arranged by size:
2518 2423 1562 1443 496 473 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 8524
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4570 6212 6547 7308 8336 1540
Size: 275 186 2569 1642 335 761 1028 2301
Fragments arranged by size:
2569 2301 1642 1028 761 335 275 186
BsiHKAI G_wGCw'C
Cuts at: 2805 3078 4521 4565 4629 5009 7158 7243 8314
Size: 273 1443 44 64 380 2149 85 1071
Cuts at: 8314 2805
Size: 3588
Fragments arranged by size:
3588 2149 1443 1071 380 273 85 64
44
BsmI GAATG_Cn'
Cuts at: 2382 4614 5872 6608 6701 2382
Size: 2232 1258 736 93 4778
Fragments arranged by size:
4778 2232 1258 736 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3278 3640 3278
Size: 362 8735
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 2284 2805 3078 3585 4521 4565
Size: 399 384 110 521 273 507 936 44
Cuts at: 4565 4629 5009 5150 5821 6416 7158 7243 8314
Size: 64 380 141 671 595 742 85 1071
Cuts at: 8314 1391
Size: 2174
Fragments arranged by size:
2174 1071 936 742 671 595 521 507
399 384 380 273 141 110 85 64
44
BspEI T'CCGG_A
Cuts at: 8760 8760
Size: 9097
BspHI T'CATG_A
Cuts at: 3960 4024 6986 8716 3960
Size: 64 2962 1730 4341
Fragments arranged by size:
4341 2962 1730 64
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5788 6452 844
Size: 4944 664 3489
Fragments arranged by size:
4944 3489 664
BsrBI CCG'CTC
Cuts at: 1394 1823 2736 6984 1394
Size: 429 913 4248 3507
Fragments arranged by size:
4248 3507 913 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7592 7766 65
Size: 1183 6344 174 1396
Fragments arranged by size:
6344 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2041 3992 4280 4569 4911 5625 7741
Size: 110 231 1951 288 289 342 714 2116
Cuts at: 7741 7928 1700
Size: 187 2869
Fragments arranged by size:
2869 2116 1951 714 342 289 288 231
187 110
BsrGI T'GTAC_A
Cuts at: 96 6363 96
Size: 6267 2830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9097
BssSI C'ACGA_G
Cuts at: 2297 7157 8169 2297
Size: 4860 1012 3225
Fragments arranged by size:
4860 3225 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3004 5652 6289 794
Size: 2210 2648 637 3602
Fragments arranged by size:
3602 2648 2210 637
BstBI TT'CG_AA
Cuts at: 2158 7901 2158
Size: 5743 3354
BstEII G'GTnAC_C
Cuts at: 1184 2521 3442 4246 5777 1184
Size: 1337 921 804 1531 4504
Fragments arranged by size:
4504 1531 1337 921 804
BstXI CCAn_nnnn'nTGG
Cuts at: 3088 3088
Size: 9097
BstYI r'GATC_y
Cuts at: 1972 2419 2667 4398 4670 5352 5395 5461 6427
Size: 447 248 1731 272 682 43 66 966
Cuts at: 6427 6794 7179 7196 8648 8734 8746 9092 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1731 1452 966 682 447 385 367
346 272 248 86 66 43 17 12
Bsu36I CC'TnA_GG
Cuts at: 3158 3158
Size: 9097
BtgI C'CryG_G
Cuts at: 513 1065 2004 2290 2469 3787 4485 5055 513
Size: 552 939 286 179 1318 698 570 4555
Fragments arranged by size:
4555 1318 939 698 570 552 286 179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5727 487
Size: 683 56 4501 3857
Fragments arranged by size:
4501 3857 683 56
BtsI GCAGTG_nn'
Cuts at: 5641 6613 7407 7427 5641
Size: 972 794 20 7311
Fragments arranged by size:
7311 972 794 20
ClaI AT'CG_AT
Cuts at: 1554 5748 1554
Size: 4194 4903
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9062
DraI TTT'AAA
Cuts at: 5263 6339 6401 5263
Size: 1076 62 7959
Fragments arranged by size:
7959 1076 62
DraIII CAC_nnn'GTG
Cuts at: 2140 3169 2140
Size: 1029 8068
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2507 4154 8104 809
Size: 507 1191 1647 3950 1802
Fragments arranged by size:
3950 1802 1647 1191 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4905 4998 6544
Size: 54 1425 50 275 18 3075 93 1546
Cuts at: 6544 7433 9062 8
Size: 889 1629 43
Fragments arranged by size:
3075 1629 1546 1425 889 275 93 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6544 1537
Size: 275 4732 4090
Fragments arranged by size:
4732 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2680 4313 4529 4589 5160 5523 7027 1353
Size: 1327 1633 216 60 571 363 1504 3423
Fragments arranged by size:
3423 1633 1504 1327 571 363 216 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2099 7684 8058 8204 387
Size: 1064 138 510 5585 374 146 1280
Fragments arranged by size:
5585 1280 1064 510 374 146 138
EcoICRI GAG'CTC
Cuts at: 2803 2803
Size: 9097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2501 2681 2888 2945 3257 3416 3582
Size: 393 869 180 207 57 312 159 166
Cuts at: 3582 3724 4283 4328 4397 4635 5750 5984 6068
Size: 142 559 45 69 238 1115 234 84
Cuts at: 6068 7160 7757 8544 8812 1239
Size: 1092 597 787 268 1524
Fragments arranged by size:
1524 1115 1092 869 787 597 559 393
312 268 238 234 207 180 166 159
142 84 69 57 45
EcoNI CCTnn'n_nnAGG
Cuts at: 3239 3239
Size: 9097
EcoO109I rG'GnC_Cy
Cuts at: 2093 2170 2171 2989 3244 3832 4845 5130 5697
Size: 77 1 818 255 588 1013 285 567
Cuts at: 5697 5922 2093
Size: 225 5268
Fragments arranged by size:
5268 1013 818 588 567 285 255 225
77 1
EcoRI G'AATT_C
Cuts at: 2549 6406 6784 2549
Size: 3857 378 4862
Fragments arranged by size:
4862 3857 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4558 5275 5552 6091 4558
Size: 717 277 539 7564
Fragments arranged by size:
7564 717 539 277
FspI TGC'GCA
Cuts at: 7603 7603
Size: 9097
HaeII r_GCGC'y
Cuts at: 1678 2072 3669 8244 1678
Size: 394 1597 4575 2531
Fragments arranged by size:
4575 2531 1597 394
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2110 2142 2258 2290 2336
Size: 32 135 32 538 32 116 32 46
Cuts at: 2336 2368 2411 2439 2443 2471 2807 2839 4829
Size: 32 43 28 4 28 336 32 1990
Cuts at: 4829 4861 5066 5098 5337 5369 5851 5883 6074
Size: 32 205 32 239 32 482 32 191
Cuts at: 6074 6106 7738 7770 7812 7844 1373
Size: 32 1632 32 42 32 2626
Fragments arranged by size:
2626 1990 1632 538 482 336 239 205
191 135 116 46 43 42 32 32
32 32 32 32 32 32 32 32
32 32 32 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3472 5893 6225 6441 6687 669
Size: 988 281 1534 2421 332 216 246 3079
Fragments arranged by size:
3079 2421 1534 988 332 281 246 216
HindIII A'AGCT_T
Cuts at: 748 3401 748
Size: 2653 6444
HpaI GTT'AAC
Cuts at: 3472 6687 3472
Size: 3215 5882
KasI G'GCGC_C
Cuts at: 2068 2068
Size: 9097
KpnI G_GTAC'C
Cuts at: 6422 6422
Size: 9097
MfeI C'AATT_G
Cuts at: 3804 6696 3804
Size: 2892 6205
MluI A'CGCG_T
Cuts at: 6358 8973 6358
Size: 2615 6482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2447 3219 3293 3730 4590 4752 5001 5565
Size: 1142 772 74 437 860 162 249 564
Cuts at: 5565 5685 8211 8395 1305
Size: 120 2526 184 2007
Fragments arranged by size:
2526 2007 1142 860 772 564 437 249
184 162 120 74
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4907 10
Size: 54 1425 343 3075 4200
Fragments arranged by size:
4200 3075 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2258 2474 4556 4802 6120 6264 7055
Size: 648 1092 216 2082 246 1318 144 791
Cuts at: 7055 7414 7573 518
Size: 359 159 2042
Fragments arranged by size:
2082 2042 1318 1092 791 648 359 246
216 159 144
MspA1I CmG'CkG
Cuts at: 1606 2659 2909 2961 4438 4611 5041 6513 7190
Size: 1053 250 52 1477 173 430 1472 677
Cuts at: 7190 8338 8583 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1477 1472 1148 1053 677 430 250
245 173 52
NaeI GCC'GGC
Cuts at: 2043 2043
Size: 9097
NarI GG'CG_CC
Cuts at: 2069 2069
Size: 9097
NcoI C'CATG_G
Cuts at: 513 1065 2004 3787 4485 513
Size: 552 939 1783 698 5125
Fragments arranged by size:
5125 1783 939 698 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9097
NgoMIV G'CCGG_C
Cuts at: 2041 2041
Size: 9097
NheI G'CTAG_C
Cuts at: 1052 3932 1052
Size: 2880 6217
NotI GC'GGCC_GC
Cuts at: 6544 6544
Size: 9097
NsiI A_TGCA'T
Cuts at: 4110 4110
Size: 9097
NspI r_CATG'y
Cuts at: 1132 1229 2510 2605 4108 4474 4687 4726 5668
Size: 97 1281 95 1503 366 213 39 942
Cuts at: 5668 6287 6455 8000 1132
Size: 619 168 1545 2229
Fragments arranged by size:
2229 1545 1503 1281 942 619 366 213
168 97 95 39
PasI CC'CwG_GG
Cuts at: 1348 6330 1348
Size: 4982 4115
PciI A'CATG_T
Cuts at: 4683 7996 4683
Size: 3313 5784
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2341 6046 6133 1108
Size: 1233 3705 87 4072
Fragments arranged by size:
4072 3705 1233 87
PfoI T'CCnGG_A
Cuts at: 5445 5927 5445
Size: 482 8615
PmeI GTTT'AAAC
Cuts at: 6401 6401
Size: 9097
PmlI CAC'GTG
Cuts at: 2260 5211 2260
Size: 2951 6146
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7166 7198 8679 8711 7166
Size: 32 1481 32 7552
Fragments arranged by size:
7552 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2093 2989 4845 5130 5697 2093
Size: 896 1856 285 567 5493
Fragments arranged by size:
5493 1856 896 567 285
PshAI GACnn'nnGTC
Cuts at: 1613 2651 1613
Size: 1038 8059
PsiI TTA'TAA
Cuts at: 6667 6667
Size: 9097
PspOMI G'GGCC_C
Cuts at: 2170 2170
Size: 9097
PstI C_TGCA'G
Cuts at: 830 5648 6350 6449 830
Size: 4818 702 99 3478
Fragments arranged by size:
4818 3478 702 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9097
PvuII CAG'CTG
Cuts at: 2659 2961 4438 4611 5041 2659
Size: 302 1477 173 430 6715
Fragments arranged by size:
6715 1477 430 302 173
RsrII CG'GwC_CG
Cuts at: 2063 2063
Size: 9097
SacI G_AGCT'C
Cuts at: 2805 2805
Size: 9097
SalI G'TCGA_C
Cuts at: 1936 5891 6439 1936
Size: 3955 548 4594
Fragments arranged by size:
4594 3955 548
SanDI GG'GwC_CC
Cuts at: 2093 2093
Size: 9097
SapI GCTCTTCn'nnn_
Cuts at: 4589 4589
Size: 9097
SbfI CC_TGCA'GG
Cuts at: 6449 6449
Size: 9097
ScaI AGT'ACT
Cuts at: 7345 9057 7345
Size: 1712 7385
SexAI A'CCwGG_T
Cuts at: 3069 3069
Size: 9097
SfcI C'TryA_G
Cuts at: 826 1045 2542 2860 4606 5296 5361 5644 6346
Size: 219 1497 318 1746 690 65 283 702
Cuts at: 6346 6445 7580 8261 8452 826
Size: 99 1135 681 191 1471
Fragments arranged by size:
1746 1497 1471 1135 702 690 681 318
283 219 191 99 65
SfoI GGC'GCC
Cuts at: 2070 2070
Size: 9097
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9097
SmaI CCC'GGG
Cuts at: 1872 6424 1872
Size: 4552 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4741 5942 6001 6217 7201 8102
Size: 197 926 2798 1201 59 216 984 901
Cuts at: 8102 8364 820
Size: 262 1553
Fragments arranged by size:
2798 1553 1201 984 926 901 262 216
197 59
SnaBI TAC'GTA
Cuts at: 493 6888 493
Size: 6395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9097
SphI G_CATG'C
Cuts at: 1132 6455 1132
Size: 5323 3774
SspI AAT'ATT
Cuts at: 5 52 2110 7021 5
Size: 47 2058 4911 2081
Fragments arranged by size:
4911 2081 2058 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9097
StyI C'CwwG_G
Cuts at: 513 1065 2004 2740 2952 3787 4152 4313 4485
Size: 552 939 736 212 835 365 161 172
Cuts at: 4485 5150 5250 5289 5900 6040 6536 513
Size: 665 100 39 611 140 496 3074
Fragments arranged by size:
3074 939 835 736 665 611 552 496
365 212 172 161 140 100 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3024 3667 4343 4771 6198 7124 7141
Size: 1255 1444 643 676 428 1427 926 17
Cuts at: 7141 7294 7479 7924 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1444 1427 1255 926 676 643 445
428 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4113 4830 6318
Size: 274 80 33 51 1443 2136 717 1488
Cuts at: 6318 6363 7343 9055 96
Size: 45 980 1712 138
Fragments arranged by size:
2136 1712 1488 1443 980 717 274 138
80 51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4370 4402 9057 9089 4370
Size: 32 4655 32 4378
Fragments arranged by size:
4655 4378 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9097
XbaI T'CTAG_A
Cuts at: 3652 5027 6433 3652
Size: 1375 1406 6316
Fragments arranged by size:
6316 1406 1375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3889 2011
Size: 1878 7219
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9097
XmaI C'CCGG_G
Cuts at: 1870 6422 1870
Size: 4552 4545
XmnI GAAnn'nnTTC
Cuts at: 5958 7226 5958
Size: 1268 7829
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2427 6905 276
Size: 53 83 186 936 893 4478 2468
Fragments arranged by size:
4478 2468 936 893 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BsiWI BstZ17I
EcoRV FseI FspAI NruI PacI PspXI PsrI SacII
SfiI SrfI SwaI