Restriction map for
pFN21AB4957
(Circular) (Six-base) MAPSORT of: pFN21AB4957.seq Check: 7816 from: 1 to: 6307
pFN21AB4957 6307 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4117 278
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3650 1937
Size: 1713 4594
Acc65I G'GTAC_C
Cuts at: 3628 3628
Size: 6307
AclI AA'CG_TT
Cuts at: 1563 4434 4807 1563
Size: 2871 373 3063
Fragments arranged by size:
3063 2871 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2775 3432 4370 5754 6022 1632
Size: 1143 657 938 1384 268 1917
Fragments arranged by size:
1917 1384 1143 938 657 268
AflII C'TTAA_G
Cuts at: 820 1017 2312 820
Size: 197 1295 4815
Fragments arranged by size:
4815 1295 197
AflIII A'CryG_T
Cuts at: 1203 5206 6183 1203
Size: 4003 977 1327
Fragments arranged by size:
4003 1327 977
AgeI A'CCGG_T
Cuts at: 5138 5138
Size: 6307
AhdI GACnn_n'nnGTC
Cuts at: 5036 5036
Size: 6307
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6307
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6307
AlwNI CAG_nnn'CTG
Cuts at: 4050 5622 4050
Size: 1572 4735
ApaLI G'TGCA_C
Cuts at: 4364 5520 4364
Size: 1156 5151
ApoI r'AATT_y
Cuts at: 1493 2031 2963 3572 3616 3842 3994 6103 1493
Size: 538 932 609 44 226 152 2109 1697
Fragments arranged by size:
2109 1697 932 609 538 226 152 44
AseI AT'TA_AT
Cuts at: 160 1032 2021 2264 3195 4861 160
Size: 872 989 243 931 1666 1606
Fragments arranged by size:
1666 1606 989 931 872 243
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
AvaI C'yCGr_G
Cuts at: 1870 1943 3632 1870
Size: 73 1689 4545
Fragments arranged by size:
4545 1689 73
BamHI G'GATC_C
Cuts at: 3637 4004 3637
Size: 367 5940
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3628 5083 618
Size: 325 217 627 1841 1455 1842
Fragments arranged by size:
1842 1841 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3626 1391
Size: 2235 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3586 928
Size: 971 1687 3649
Fragments arranged by size:
3649 1687 971
BbvCI CC'TCA_GC
Cuts at: 2496 3083 2496
Size: 587 5720
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4498 4532 1903
Size: 34 2561 34 3678
Fragments arranged by size:
3678 2561 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2413 2428 3550 4199 5409 2413
Size: 15 1122 649 1210 3311
Fragments arranged by size:
3311 1210 1122 649 15
BclI T'GATC_A
Cuts at: 1548 2806 1548
Size: 1258 5049
BfrBI ATG'CAT
Cuts at: 2255 2255
Size: 6307
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4918 136
Size: 107 122 71 631 605 3246 1525
Fragments arranged by size:
3246 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2113 3211 6302 2113
Size: 1098 3091 2118
Fragments arranged by size:
3091 2118 1098
BlpI GC'TnA_GC
Cuts at: 3724 3724
Size: 6307
Bme1580I G_kGCm'C
Cuts at: 1790 4368 5524 1790
Size: 2578 1156 2573
Fragments arranged by size:
2578 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6307
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2038 4038 4996
Size: 416 53 83 399 24 621 2000 958
Cuts at: 4996 442
Size: 1753
Fragments arranged by size:
2000 1753 958 621 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2399 2961 4967 1239
Size: 1160 562 2006 2579
Fragments arranged by size:
2579 2006 1160 562
Bpu10I CC'TnA_GC
Cuts at: 2496 3083 2496
Size: 587 5720
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4432 5297 5595 4432
Size: 865 298 5144
Fragments arranged by size:
5144 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2655 4970 882
Size: 617 1156 2315 2219
Fragments arranged by size:
2315 2219 1156 617
BsaAI yAC'GTr
Cuts at: 493 1204 4098 493
Size: 711 2894 2702
Fragments arranged by size:
2894 2702 711
BsaBI GATnn'nnATC
Cuts at: 2379 6301 2379
Size: 3922 2385
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4114 4496 5131 275
Size: 53 83 186 936 2581 382 635 1451
Fragments arranged by size:
2581 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4740 5138 5412 5559 5970 4740
Size: 398 274 147 411 5077
Fragments arranged by size:
5077 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3387 3411 3417 3441 1178
Size: 30 165 30 1984 24 6 24 4044
Fragments arranged by size:
4044 1984 165 30 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3408 1186
Size: 2222 4085
BseYI C'CCAG_C
Cuts at: 3334 5510 6006 3334
Size: 2176 496 3635
Fragments arranged by size:
3635 2176 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5734
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3757 4518 5546 1540
Size: 275 186 1756 761 1028 2301
Fragments arranged by size:
2301 1756 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4368 4453 5524 4368
Size: 85 1071 5151
Fragments arranged by size:
5151 1071 85
BsmI GAATG_Cn'
Cuts at: 2255 3818 3911 2255
Size: 1563 93 4651
Fragments arranged by size:
4651 1563 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2417 3119 2417
Size: 702 5605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3626 4368 4453 5524 1391
Size: 399 1836 742 85 1071 2174
Fragments arranged by size:
2174 1836 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5970 5970
Size: 6307
BspHI T'CATG_A
Cuts at: 4196 5926 4196
Size: 1730 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2859 3662 844
Size: 2015 803 3489
Fragments arranged by size:
3489 2015 803
BsrBI CCG'CTC
Cuts at: 1394 1823 4194 1394
Size: 429 2371 3507
Fragments arranged by size:
3507 2371 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4802 4976 65
Size: 1183 3554 174 1396
Fragments arranged by size:
3554 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4951 5138 1700
Size: 110 3141 187 2869
Fragments arranged by size:
3141 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6307
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6307
BssSI C'ACGA_G
Cuts at: 4367 5379 4367
Size: 1012 5295
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6307
BstBI TT'CG_AA
Cuts at: 3577 5111 3577
Size: 1534 4773
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6307
BstXI CCAn_nnnn'nTGG
Cuts at: 3563 3563
Size: 6307
BstYI r'GATC_y
Cuts at: 1972 2113 2221 2707 3211 3637 4004 4389 4406
Size: 141 108 486 504 426 367 385 17
Cuts at: 4406 5858 5944 5956 6302 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 504 486 426 385 367 346
141 108 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2576 2576
Size: 6307
BtgI C'CryG_G
Cuts at: 513 1065 2884 513
Size: 552 1819 3936
Fragments arranged by size:
3936 1819 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5568
Fragments arranged by size:
5568 683 56
BtsI GCAGTG_nn'
Cuts at: 3823 4617 4637 3823
Size: 794 20 5493
Fragments arranged by size:
5493 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6272
DraI TTT'AAA
Cuts at: 3611 3611
Size: 6307
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5314 809
Size: 507 3998 1802
Fragments arranged by size:
3998 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3754 4643 6272
Size: 54 1425 50 275 18 1924 889 1629
Cuts at: 6272 8
Size: 43
Fragments arranged by size:
1924 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3754 1537
Size: 275 1942 4090
Fragments arranged by size:
4090 1942 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 4237 1353
Size: 792 2092 3423
Fragments arranged by size:
3423 2092 792
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4894 5268 5414 387
Size: 1064 138 3305 374 146 1280
Fragments arranged by size:
3305 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 2775 2961 3432 4370 4967 5754
Size: 393 767 376 186 471 938 597 787
Cuts at: 5754 6022 1239
Size: 268 1524
Fragments arranged by size:
1524 938 787 767 597 471 393 376
268 186
EcoNI CCTnn'n_nnAGG
Cuts at: 2669 2669
Size: 6307
EcoRI G'AATT_C
Cuts at: 2031 3616 3994 2031
Size: 1585 378 4344
Fragments arranged by size:
4344 1585 378
FspI TGC'GCA
Cuts at: 4813 4813
Size: 6307
HaeII r_GCGC'y
Cuts at: 1678 5454 1678
Size: 3776 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2206 2238 2432 2464 2603
Size: 32 135 32 634 32 194 32 139
Cuts at: 2603 2635 2786 2818 4948 4980 5022 5054 1373
Size: 32 151 32 2130 32 42 32 2626
Fragments arranged by size:
2626 2130 634 194 151 139 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3651 3897 669
Size: 988 281 1713 246 3079
Fragments arranged by size:
3079 1713 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2693 748
Size: 1945 4362
HpaI GTT'AAC
Cuts at: 3897 3897
Size: 6307
KpnI G_GTAC'C
Cuts at: 3632 3632
Size: 6307
MfeI C'AATT_G
Cuts at: 2076 3906 2076
Size: 1830 4477
MluI A'CGCG_T
Cuts at: 6183 6183
Size: 6307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2277 3250 3397 5421 5605 1305
Size: 972 973 147 2024 184 2007
Fragments arranged by size:
2024 2007 973 972 184 147
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4485
Fragments arranged by size:
4485 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3561 4265 4624 4783 518
Size: 648 2395 704 359 159 2042
Fragments arranged by size:
2395 2042 704 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3723 4400 5548 5793 1606
Size: 2117 677 1148 245 2120
Fragments arranged by size:
2120 2117 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5755
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6307
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6307
NotI GC'GGCC_GC
Cuts at: 3754 3754
Size: 6307
NsiI A_TGCA'T
Cuts at: 2257 2257
Size: 6307
NspI r_CATG'y
Cuts at: 1132 1229 3506 3665 5210 1132
Size: 97 2277 159 1545 2229
Fragments arranged by size:
2277 2229 1545 159 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6307
PciI A'CATG_T
Cuts at: 5206 5206
Size: 6307
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2050 3549 1108
Size: 902 40 1499 3866
Fragments arranged by size:
3866 1499 902 40
PfoI T'CCnGG_A
Cuts at: 2415 2464 2415
Size: 49 6258
PmeI GTTT'AAAC
Cuts at: 3611 3611
Size: 6307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4376 4408 5889 5921 4376
Size: 32 1481 32 4762
Fragments arranged by size:
4762 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6307
PsiI TTA'TAA
Cuts at: 3877 3877
Size: 6307
PstI C_TGCA'G
Cuts at: 830 3391 3659 830
Size: 2561 268 3478
Fragments arranged by size:
3478 2561 268
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6307
SalI G'TCGA_C
Cuts at: 1936 3649 1936
Size: 1713 4594
SbfI CC_TGCA'GG
Cuts at: 3659 3659
Size: 6307
ScaI AGT'ACT
Cuts at: 2363 4555 6267 2363
Size: 2192 1712 2403
Fragments arranged by size:
2403 2192 1712
SfcI C'TryA_G
Cuts at: 826 1045 3387 3655 4790 5471 5662 826
Size: 219 2342 268 1135 681 191 1471
Fragments arranged by size:
2342 1471 1135 681 268 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6307
SmaI CCC'GGG
Cuts at: 1872 3634 1872
Size: 1762 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2312 4411 5312 5574 820
Size: 197 926 369 2099 901 262 1553
Fragments arranged by size:
2099 1553 926 901 369 262 197
SnaBI TAC'GTA
Cuts at: 493 4098 493
Size: 3605 2702
SpeI A'CTAG_T
Cuts at: 152 2204 152
Size: 2052 4255
SphI G_CATG'C
Cuts at: 1132 3506 3665 1132
Size: 2374 159 3774
Fragments arranged by size:
3774 2374 159
SspI AAT'ATT
Cuts at: 5 52 2744 4231 5
Size: 47 2692 1487 2081
Fragments arranged by size:
2692 2081 1487 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6307
StyI C'CwwG_G
Cuts at: 513 1065 3077 3746 513
Size: 552 2012 669 3074
Fragments arranged by size:
3074 2012 669 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4334 4351 4504 4689 5134 325
Size: 1255 2754 17 153 185 445 1498
Fragments arranged by size:
2754 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2207 2361 3494
Size: 274 80 33 51 1443 230 154 1133
Cuts at: 3494 4553 6265 96
Size: 1059 1712 138
Fragments arranged by size:
1712 1443 1133 1059 274 230 154 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3391 3423 6267 6299 3391
Size: 32 2844 32 3399
Fragments arranged by size:
3399 2844 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6307
XbaI T'CTAG_A
Cuts at: 2626 3643 2626
Size: 1017 5290
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6307
XmaI C'CCGG_G
Cuts at: 1870 3632 1870
Size: 1762 4545
XmnI GAAnn'nnTTC
Cuts at: 4436 4436
Size: 6307
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4115 276
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI