Restriction map for
pFN21AB4945
(Circular) (Six-base) MAPSORT of: pFN21AB4945.seq Check: 9021 from: 1 to: 5641
pFN21AB4945 5641 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2409 2786 2984 1937
Size: 472 377 198 4594
Fragments arranged by size:
4594 472 377 198
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 3704 5088 5356 1632
Size: 695 1377 1384 268 1917
Fragments arranged by size:
1917 1384 1377 695 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 2334 4540 5517 1203
Size: 1131 2206 977 1327
Fragments arranged by size:
2206 1327 1131 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2255 2287 2255
Size: 32 5609
AlwNI CAG_nnn'CTG
Cuts at: 2305 3384 4956 2305
Size: 1079 1572 2990
Fragments arranged by size:
2990 1572 1079
ApaI G_GGCC'C
Cuts at: 2543 2543
Size: 5641
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2176 2950 3176 3328 5437 1493
Size: 683 774 226 152 2109 1697
Fragments arranged by size:
2109 1697 774 683 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2966 1870
Size: 73 1023 4545
Fragments arranged by size:
4545 1023 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2425 2962 4417 618
Size: 325 217 627 638 537 1455 1842
Fragments arranged by size:
1842 1455 638 627 537 325 217
BanII G_rGCy'C
Cuts at: 1391 2190 2543 2960 1391
Size: 799 353 417 4072
Fragments arranged by size:
4072 799 417 353
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2783 928
Size: 971 884 3786
Fragments arranged by size:
3786 971 884
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2543 2702 3702 4858 1790
Size: 753 159 1000 1156 2573
Fragments arranged by size:
2573 1156 1000 753 159
BmgBI CAC'GTC
Cuts at: 1318 2044 1318
Size: 726 4915
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2712 3372 4330
Size: 416 53 83 399 24 1295 660 958
Cuts at: 4330 442
Size: 1753
Fragments arranged by size:
1753 1295 958 660 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2891 4301 1239
Size: 935 717 1410 2579
Fragments arranged by size:
2579 1410 935 717
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2744 3766 4631 4929 2744
Size: 1022 865 298 3456
Fragments arranged by size:
3456 1022 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2059 2242 2335 3432 493
Size: 711 855 183 93 1097 2702
Fragments arranged by size:
2702 1097 855 711 183 93
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2685 3448 3830 4465
Size: 53 83 186 936 1152 763 382 635
Cuts at: 4465 275
Size: 1451
Fragments arranged by size:
1451 1152 936 763 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2661 4074 4472 4746 4893 5304 2661
Size: 1413 398 274 147 411 2998
Fragments arranged by size:
2998 1413 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2561 2591 1178
Size: 30 165 30 597 30 531 30 4228
Fragments arranged by size:
4228 597 531 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2027 2635 2638 1186
Size: 838 3 608 3 4189
Fragments arranged by size:
4189 838 608 3 3
BseYI C'CCAG_C
Cuts at: 2608 2855 4844 5340 2608
Size: 247 1989 496 2909
Fragments arranged by size:
2909 1989 496 247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 2513 2906 1163
Size: 573 582 195 393 3898
Fragments arranged by size:
3898 582 573 393 195
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 2190 2247 3702 3787 4858 2028
Size: 162 57 1455 85 1071 2811
Fragments arranged by size:
2811 1455 1071 162 85 57
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2190 2247 2543 2702 2960 3702
Size: 399 238 162 57 296 159 258 742
Cuts at: 3702 3787 4858 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 296 258 238 162
159 85 57
BspEI T'CCGG_A
Cuts at: 2661 5304 2661
Size: 2643 2998
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2080 2580 2996 844
Size: 1236 500 416 3489
Fragments arranged by size:
3489 1236 500 416
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4285 4472 1700
Size: 110 2475 187 2869
Fragments arranged by size:
2869 2475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2728 2869 2938 2287
Size: 441 141 69 4990
Fragments arranged by size:
4990 441 141 69
BstYI r'GATC_y
Cuts at: 1972 2487 2971 3338 3723 3740 5192 5278 5290
Size: 515 484 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 515 484 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2210 2210
Size: 5641
BtgI C'CryG_G
Cuts at: 513 1065 2004 2050 2280 2550 2643 2931 513
Size: 552 939 46 230 270 93 288 3223
Fragments arranged by size:
3223 939 552 288 270 230 93 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 3951 3971 3157
Size: 794 20 4827
Fragments arranged by size:
4827 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2552 4648 809
Size: 507 1236 2096 1802
Fragments arranged by size:
2096 1802 1236 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2277 2479
Size: 54 1425 50 275 18 243 204 202
Cuts at: 2479 3088 3977 5606 8
Size: 609 889 1629 43
Fragments arranged by size:
1629 1425 889 609 275 243 204 202
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3571 1353
Size: 2218 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2188 2188
Size: 5641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2327 2891 3704 4301 5088 5356
Size: 393 542 153 564 813 597 787 268
Cuts at: 5356 1239
Size: 1524
Fragments arranged by size:
1524 813 787 597 564 542 393 268
153
EcoO109I rG'GnC_Cy
Cuts at: 2395 2540 2707 2908 2395
Size: 145 167 201 5128
Fragments arranged by size:
5128 201 167 145
EcoRI G'AATT_C
Cuts at: 2176 2950 3328 2176
Size: 774 378 4489
Fragments arranged by size:
4489 774 378
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2561 2593 2614 2646 4282
Size: 32 135 32 989 32 21 32 1636
Cuts at: 4282 4314 4356 4388 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1636 989 135 42 32 32 32
32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2592 4755 4939 1305
Size: 1287 2163 184 2007
Fragments arranged by size:
2163 2007 1287 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 10
Size: 54 1425 343 447 3372
Fragments arranged by size:
3372 1425 447 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 2663 3599 3958 4117 518
Size: 648 1119 378 936 359 159 2042
Fragments arranged by size:
2042 1119 936 648 378 359 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2086 2636 2869 3057 3734 4882 5127
Size: 446 34 550 233 188 677 1148 245
Cuts at: 5127 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 550 446 245 233 188
34
NcoI C'CATG_G
Cuts at: 513 1065 2004 2280 513
Size: 552 939 276 3874
Fragments arranged by size:
3874 939 552 276
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2527 2999 4544 1132
Size: 97 1298 472 1545 2229
Fragments arranged by size:
2229 1545 1298 472 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PfoI T'CCnGG_A
Cuts at: 2190 2388 2190
Size: 198 5443
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2395 2707 2908 2395
Size: 312 201 5128
Fragments arranged by size:
5128 312 201
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PspOMI G'GGCC_C
Cuts at: 2539 2539
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2091 2808 2993 830
Size: 1261 717 185 3478
Fragments arranged by size:
3478 1261 717 185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2869 2869
Size: 5641
SacI G_AGCT'C
Cuts at: 2190 2190
Size: 5641
SacII CC_GC'GG
Cuts at: 2053 2053
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2087 2804 2989 4124 4805 4996 826
Size: 219 1042 717 185 1135 681 191 1471
Fragments arranged by size:
1471 1135 1042 717 681 219 191 185
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2759 3745 4646 4908 820
Size: 197 926 816 986 901 262 1553
Fragments arranged by size:
1553 986 926 901 816 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 2208 1517
Size: 691 4950
StyI C'CwwG_G
Cuts at: 513 1065 2004 2280 3080 513
Size: 552 939 276 800 3074
Fragments arranged by size:
3074 939 800 552 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2342 3668 3685 3838 4023 4468 325
Size: 1255 762 1326 17 153 185 445 1498
Fragments arranged by size:
1498 1326 1255 762 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3887 5599 96
Size: 274 80 33 51 1443 1910 1712 138
Fragments arranged by size:
1910 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 5601 5633 2000
Size: 32 3569 32 2008
Fragments arranged by size:
3569 2008 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2687 2782 1316
Size: 1371 95 4175
Fragments arranged by size:
4175 1371 95
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI