Restriction map for
pFN21AB4940
(Circular) (Six-base) MAPSORT of: pFN21AB4940.seq Check: 7623 from: 1 to: 6142
pFN21AB4940 6142 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2105 2453 3261 2105
Size: 348 808 4986
Fragments arranged by size:
4986 808 348
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3952 278
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3485 1937
Size: 1548 4594
Acc65I G'GTAC_C
Cuts at: 3463 3463
Size: 6142
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2717 4205 5589 5857 1632
Size: 1085 1488 1384 268 1917
Fragments arranged by size:
1917 1488 1384 1085 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 5041 6018 1203
Size: 3838 977 1327
Fragments arranged by size:
3838 1327 977
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 2106 2961 4871 2106
Size: 855 1910 3377
Fragments arranged by size:
3377 1910 855
AleI CACnn'nnGTG
Cuts at: 1166 3195 3267 1166
Size: 2029 72 4041
Fragments arranged by size:
4041 2029 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2708 1184
Size: 1524 4618
AlwNI CAG_nnn'CTG
Cuts at: 2991 3885 5457 2991
Size: 894 1572 3676
Fragments arranged by size:
3676 1572 894
ApaI G_GGCC'C
Cuts at: 3042 3042
Size: 6142
ApaLI G'TGCA_C
Cuts at: 2857 4199 5355 2857
Size: 1342 1156 3644
Fragments arranged by size:
3644 1342 1156
ApoI r'AATT_y
Cuts at: 1493 3311 3451 3677 3829 5938 1493
Size: 1818 140 226 152 2109 1697
Fragments arranged by size:
2109 1818 1697 226 152 140
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 2482 2590 3034 3467 1870
Size: 73 539 108 444 433 4545
Fragments arranged by size:
4545 539 444 433 108 73
BamHI G'GATC_C
Cuts at: 3472 3839 3472
Size: 367 5775
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 2269 2491 2794 3360
Size: 325 217 627 330 152 222 303 566
Cuts at: 3360 3463 4918 618
Size: 103 1455 1842
Fragments arranged by size:
1842 1455 627 566 330 325 303 222
217 152 103
BanII G_rGCy'C
Cuts at: 1391 2148 2588 2613 3042 3108 3141 3461 1391
Size: 757 440 25 429 66 33 320 4072
Fragments arranged by size:
4072 757 440 429 320 66 33 25
BbeI G_GCGC'C
Cuts at: 2273 2495 2798 2273
Size: 222 303 5617
Fragments arranged by size:
5617 303 222
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2105 3163 928
Size: 971 206 1058 3907
Fragments arranged by size:
3907 1058 971 206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4333 4367 1903
Size: 34 2396 34 3678
Fragments arranged by size:
3678 2396 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4034 5244 4034
Size: 1210 4932
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2246 2592 2772
Size: 107 122 71 631 605 574 346 180
Cuts at: 2772 4753 136
Size: 1981 1525
Fragments arranged by size:
1981 1525 631 605 574 346 180 122
107 71
BglII A'GATC_T
Cuts at: 6137 6137
Size: 6142
BlpI GC'TnA_GC
Cuts at: 2708 2978 3559 2708
Size: 270 581 5291
Fragments arranged by size:
5291 581 270
Bme1580I G_kGCm'C
Cuts at: 1790 2122 2828 2861 3042 3363 4203 5359 1790
Size: 332 706 33 181 321 840 1156 2573
Fragments arranged by size:
2573 1156 840 706 332 321 181 33
BmgBI CAC'GTC
Cuts at: 1318 2581 1318
Size: 1263 4879
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2165 3301 3873
Size: 416 53 83 399 24 748 1136 572
Cuts at: 3873 4831 442
Size: 958 1753
Fragments arranged by size:
1753 1136 958 748 572 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6142
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 2534 2866 2164
Size: 370 332 5440
Fragments arranged by size:
5440 370 332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 4802 1239
Size: 1007 2556 2579
Fragments arranged by size:
2579 2556 1007
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2987 3227 4267 5132 5430 2987
Size: 240 1040 865 298 3699
Fragments arranged by size:
3699 1040 865 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2282 2854 4805 882
Size: 617 783 572 1951 2219
Fragments arranged by size:
2219 1951 783 617 572
BsaAI yAC'GTr
Cuts at: 493 1204 2823 3933 493
Size: 711 1619 1110 2702
Fragments arranged by size:
2702 1619 1110 711
BsaBI GATnn'nnATC
Cuts at: 6136 6136
Size: 6142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2270 2492 2702 2795
Size: 53 83 186 936 737 222 210 93
Cuts at: 2795 3949 4331 4966 275
Size: 1154 382 635 1451
Fragments arranged by size:
1451 1154 936 737 635 382 222 210
186 93 83 53
BsaWI w'CCGG_w
Cuts at: 2353 4575 4973 5247 5394 5805 2353
Size: 2222 398 274 147 411 2690
Fragments arranged by size:
2690 2222 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2335 2365 1178
Size: 30 165 30 932 30 4955
Fragments arranged by size:
4955 932 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2309 2386 2507 3149 1186
Size: 970 153 77 121 642 4179
Fragments arranged by size:
4179 970 642 153 121 77
BseYI C'CCAG_C
Cuts at: 2939 5345 5841 2939
Size: 2406 496 3240
Fragments arranged by size:
3240 2406 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2892 1163
Size: 573 1156 4413
Fragments arranged by size:
4413 1156 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2185 2211 2903 3164 3391 3592
Size: 275 186 184 26 692 261 227 201
Cuts at: 3592 4353 5381 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 692 275 261 227 201
186 184 26
BsiHKAI G_wGCw'C
Cuts at: 2148 2613 2861 3108 4203 4288 5359 2148
Size: 465 248 247 1095 85 1071 2931
Fragments arranged by size:
2931 1095 1071 465 248 247 85
BsmI GAATG_Cn'
Cuts at: 3653 3746 3653
Size: 93 6049
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2122 2148 2588 2613 2828 2861 3042
Size: 399 332 26 440 25 215 33 181
Cuts at: 3042 3108 3141 3363 3461 4203 4288 5359 1391
Size: 66 33 222 98 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 440 399 332 222 215
181 98 85 66 33 33 26 25
BspEI T'CCGG_A
Cuts at: 2353 5805 2353
Size: 3452 2690
BspHI T'CATG_A
Cuts at: 4031 5761 4031
Size: 1730 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2105 2453 3261 3497 844
Size: 1261 348 808 236 3489
Fragments arranged by size:
3489 1261 808 348 236
BsrBI CCG'CTC
Cuts at: 1394 1823 2232 2537 3091 3321 4029 1394
Size: 429 409 305 554 230 708 3507
Fragments arranged by size:
3507 708 554 429 409 305 230
BsrDI GCAATG_nn'
Cuts at: 65 1248 2896 4637 4811 65
Size: 1183 1648 1741 174 1396
Fragments arranged by size:
1741 1648 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2240 2898 2902 4786 4973 1700
Size: 110 430 658 4 1884 187 2869
Fragments arranged by size:
2869 1884 658 430 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 2637 96
Size: 2541 3601
BssHII G'CGCG_C
Cuts at: 1925 2433 1925
Size: 508 5634
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6142
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6142
BstXI CCAn_nnnn'nTGG
Cuts at: 2224 2224
Size: 6142
BstYI r'GATC_y
Cuts at: 1972 3472 3839 4224 4241 5693 5779 5791 6137
Size: 1500 367 385 17 1452 86 12 346
Cuts at: 6137 1972
Size: 1977
Fragments arranged by size:
1977 1500 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2211 513
Size: 552 1146 4444
Fragments arranged by size:
4444 1146 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5403
Fragments arranged by size:
5403 683 56
BtsI GCAGTG_nn'
Cuts at: 2102 3658 4452 4472 2102
Size: 1556 794 20 3772
Fragments arranged by size:
3772 1556 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DraIII CAC_nnn'GTG
Cuts at: 2785 2785
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2036 5149 809
Size: 507 720 3113 1802
Fragments arranged by size:
3113 1802 720 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2182 2214 2364
Size: 54 1425 50 275 18 352 32 150
Cuts at: 2364 2604 2618 2893 2900 3589 4478 6107 8
Size: 240 14 275 7 689 889 1629 43
Fragments arranged by size:
1629 1425 889 689 352 275 275 240
150 54 50 43 32 18 14 7
EagI C'GGCC_G
Cuts at: 1537 1812 2182 2900 3589 1537
Size: 275 370 718 689 4090
Fragments arranged by size:
4090 718 689 370 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2404 2857 3425 4072 1353
Size: 1051 453 568 647 3423
Fragments arranged by size:
3423 1051 647 568 453
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2250 2523 2582 2634 4729 5103
Size: 1064 138 661 273 59 52 2095 374
Cuts at: 5103 5249 387
Size: 146 1280
Fragments arranged by size:
2095 1280 1064 661 374 273 146 138
59 52
EcoICRI GAG'CTC
Cuts at: 2146 2611 3106 2146
Size: 465 495 5182
Fragments arranged by size:
5182 495 465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2717 4205 4802 5589 5857 1239
Size: 393 614 471 1488 597 787 268 1524
Fragments arranged by size:
1524 1488 787 614 597 471 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3038 3038
Size: 6142
EcoRI G'AATT_C
Cuts at: 3451 3829 3451
Size: 378 5764
FspI TGC'GCA
Cuts at: 4648 4648
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 2273 2495 2715 2798 2874 5289 1678
Size: 595 222 220 83 76 2415 2531
Fragments arranged by size:
2531 2415 595 222 220 83 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2092 2124 2335 2367 2868
Size: 32 135 32 520 32 211 32 501
Cuts at: 2868 2900 2947 2979 3223 3255 4783 4815 4857
Size: 32 47 32 244 32 1528 32 42
Cuts at: 4857 4889 1373
Size: 32 2626
Fragments arranged by size:
2626 1528 520 501 244 211 135 47
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3486 3732 669
Size: 988 281 1548 246 3079
Fragments arranged by size:
3079 1548 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6142
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KasI G'GCGC_C
Cuts at: 2269 2491 2794 2269
Size: 222 303 5617
Fragments arranged by size:
5617 303 222
KpnI G_GTAC'C
Cuts at: 3467 3467
Size: 6142
MfeI C'AATT_G
Cuts at: 3741 3741
Size: 6142
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2570 3387 5256 5440 1305
Size: 1265 817 1869 184 2007
Fragments arranged by size:
2007 1869 1265 817 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2216 2895 10
Size: 54 1425 343 384 679 3257
Fragments arranged by size:
3257 1425 679 384 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3195 3267 4100 4459 4618 518
Size: 648 2029 72 833 359 159 2042
Fragments arranged by size:
2042 2029 833 648 359 159 72
MspA1I CmG'CkG
Cuts at: 1606 2133 2421 3558 4235 5383 5628 1606
Size: 527 288 1137 677 1148 245 2120
Fragments arranged by size:
2120 1148 1137 677 527 288 245
NaeI GCC'GGC
Cuts at: 2904 2904
Size: 6142
NarI GG'CG_CC
Cuts at: 2270 2492 2795 2270
Size: 222 303 5617
Fragments arranged by size:
5617 303 222
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5590
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6142
NgoMIV G'CCGG_C
Cuts at: 2902 2902
Size: 6142
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6142
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 3130 3500 5045 1132
Size: 97 1901 370 1545 2229
Fragments arranged by size:
2229 1901 1545 370 97
PasI CC'CwG_GG
Cuts at: 1348 2923 2959 1348
Size: 1575 36 4531
Fragments arranged by size:
4531 1575 36
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2785 1108
Size: 1677 4465
PfoI T'CCnGG_A
Cuts at: 2310 2310
Size: 6142
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PmlI CAC'GTG
Cuts at: 2823 2823
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6142
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PspOMI G'GGCC_C
Cuts at: 3038 3038
Size: 6142
PstI C_TGCA'G
Cuts at: 830 2450 3494 830
Size: 1620 1044 3478
Fragments arranged by size:
3478 1620 1044
PvuI CG_AT'CG
Cuts at: 2001 3164 2001
Size: 1163 4979
SacI G_AGCT'C
Cuts at: 2148 2613 3108 2148
Size: 465 495 5182
Fragments arranged by size:
5182 495 465
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 4390 6102 4390
Size: 1712 4430
SfcI C'TryA_G
Cuts at: 826 1045 2446 3490 4625 5306 5497 826
Size: 219 1401 1044 1135 681 191 1471
Fragments arranged by size:
1471 1401 1135 1044 681 219 191
SfoI GGC'GCC
Cuts at: 2271 2493 2796 2271
Size: 222 303 5617
Fragments arranged by size:
5617 303 222
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 2484 3036 3469 1872
Size: 612 552 433 4545
Fragments arranged by size:
4545 612 552 433
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2966 3242 4246 5147 5409 820
Size: 197 926 1023 276 1004 901 262 1553
Fragments arranged by size:
1553 1023 1004 926 901 276 262 197
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 3130 3500 1132
Size: 1998 370 3774
Fragments arranged by size:
3774 1998 370
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 2514 1517
Size: 997 5145
StyI C'CwwG_G
Cuts at: 513 1065 3581 513
Size: 552 2516 3074
Fragments arranged by size:
3074 2516 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2306 2731 2876 3203 3405 4169 4186
Size: 1255 726 425 145 327 202 764 17
Cuts at: 4186 4339 4524 4969 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 764 726 445 425 327 202
185 153 145 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2637 4388 6100
Size: 274 80 33 51 1443 660 1751 1712
Cuts at: 6100 96
Size: 138
Fragments arranged by size:
1751 1712 1443 660 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2901 2933 6102 6134 2901
Size: 32 3169 32 2909
Fragments arranged by size:
3169 2909 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6142
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2870 2870
Size: 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 2482 3034 3467 1870
Size: 612 552 433 4545
Fragments arranged by size:
4545 612 552 433
XmnI GAAnn'nnTTC
Cuts at: 4271 4271
Size: 6142
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3950 276
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PpuMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI