Restriction map for
pFN21AB4917
(Circular) (Six-base) MAPSORT of: pFN21AB4917.seq Check: 3956 from: 1 to: 5674
pFN21AB4917 5674 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2084 2357 2084
Size: 273 5401
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2919 3017 1937
Size: 982 98 4594
Fragments arranged by size:
4594 982 98
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2405 2756 2879 3737 5121 5389 1632
Size: 773 351 123 858 1384 268 1917
Fragments arranged by size:
1917 1384 858 773 351 268 123
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 2682 4573 5550 1203
Size: 1479 1891 977 1327
Fragments arranged by size:
1891 1479 1327 977
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 2078 1166
Size: 912 4762
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5674
AlwNI CAG_nnn'CTG
Cuts at: 2305 3417 4989 2305
Size: 1112 1572 2990
Fragments arranged by size:
2990 1572 1112
ApaI G_GGCC'C
Cuts at: 2846 2846
Size: 5674
ApaLI G'TGCA_C
Cuts at: 3731 4887 3731
Size: 1156 4518
ApoI r'AATT_y
Cuts at: 1493 2983 3209 3361 5470 1493
Size: 1490 226 152 2109 1697
Fragments arranged by size:
2109 1697 1490 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2237 2573 2999 1870
Size: 73 294 336 426 4545
Fragments arranged by size:
4545 426 336 294 73
BamHI G'GATC_C
Cuts at: 2805 3004 3371 2805
Size: 199 367 5108
Fragments arranged by size:
5108 367 199
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2345 2468 2864 2995 4450
Size: 325 217 627 558 123 396 131 1455
Cuts at: 4450 618
Size: 1842
Fragments arranged by size:
1842 1455 627 558 396 325 217 131
123
BanII G_rGCy'C
Cuts at: 1391 2846 2993 1391
Size: 1455 147 4072
Fragments arranged by size:
4072 1455 147
BbeI G_GCGC'C
Cuts at: 2868 2868
Size: 5674
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2759 928
Size: 971 860 3843
Fragments arranged by size:
3843 971 860
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3865 3899 1903
Size: 34 1928 34 3678
Fragments arranged by size:
3678 1928 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2726 4285 136
Size: 107 122 71 631 605 1054 1559 1525
Fragments arranged by size:
1559 1525 1054 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5669 5669
Size: 5674
BlpI GC'TnA_GC
Cuts at: 3091 3091
Size: 5674
Bme1580I G_kGCm'C
Cuts at: 1790 2473 2846 3735 4891 1790
Size: 683 373 889 1156 2573
Fragments arranged by size:
2573 1156 889 683 373
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5674
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3405 4363 442
Size: 416 53 83 399 24 1988 958 1753
Fragments arranged by size:
1988 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2475 2747 4334 1239
Size: 1236 272 1587 2579
Fragments arranged by size:
2579 1587 1236 272
Bpu10I CC'TnA_GC
Cuts at: 2588 2588
Size: 5674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3799 4664 4962 3799
Size: 865 298 4511
Fragments arranged by size:
4511 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2564 4337 882
Size: 617 1065 1773 2219
Fragments arranged by size:
2219 1773 1065 617
BsaAI yAC'GTr
Cuts at: 493 1204 2683 3465 493
Size: 711 1479 782 2702
Fragments arranged by size:
2702 1479 782 711
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2657 2781 2865 3481
Size: 53 83 186 936 1124 124 84 616
Cuts at: 3481 3863 4498 275
Size: 382 635 1451
Fragments arranged by size:
1451 1124 936 635 616 382 186 124
84 83 53
BsaWI w'CCGG_w
Cuts at: 4107 4505 4779 4926 5337 4107
Size: 398 274 147 411 4444
Fragments arranged by size:
4444 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2199 2229 1178
Size: 30 165 30 796 30 4623
Fragments arranged by size:
4623 796 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2678 1186
Size: 1492 4182
BseYI C'CCAG_C
Cuts at: 2472 2517 2644 2708 2947 4877 5373 2472
Size: 45 127 64 239 1930 496 2773
Fragments arranged by size:
2773 1930 496 239 127 64 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3124 3885 4913 1540
Size: 275 186 1123 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3735 3820 4891 3735
Size: 85 1071 4518
Fragments arranged by size:
4518 1071 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2473 2846 2993 3735 3820 4891 1391
Size: 399 683 373 147 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 683 399 373 147 85
BspEI T'CCGG_A
Cuts at: 5337 5337
Size: 5674
BspHI T'CATG_A
Cuts at: 3563 5293 3563
Size: 1730 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2084 2357 2541 3029 844
Size: 1240 273 184 488 3489
Fragments arranged by size:
3489 1240 488 273 184
BsrBI CCG'CTC
Cuts at: 1394 1823 2178 2623 3561 1394
Size: 429 355 445 938 3507
Fragments arranged by size:
3507 938 445 429 355
BsrDI GCAATG_nn'
Cuts at: 65 1248 2412 2550 4169 4343 65
Size: 1183 1164 138 1619 174 1396
Fragments arranged by size:
1619 1396 1183 1164 174 138
BsrFI r'CCGG_y
Cuts at: 1700 1810 2555 2696 4318 4505 1700
Size: 110 745 141 1622 187 2869
Fragments arranged by size:
2869 1622 745 187 141 110
BsrGI T'GTAC_A
Cuts at: 96 2139 96
Size: 2043 3631
BssHII G'CGCG_C
Cuts at: 1925 2877 1925
Size: 952 4722
BssSI C'ACGA_G
Cuts at: 2143 2837 3734 4746 2143
Size: 694 897 1012 3071
Fragments arranged by size:
3071 1012 897 694
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5674
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstXI CCAn_nnnn'nTGG
Cuts at: 2095 2254 2374 2095
Size: 159 120 5395
Fragments arranged by size:
5395 159 120
BstYI r'GATC_y
Cuts at: 1972 2388 2430 2805 2826 3004 3371 3756 3773
Size: 416 42 375 21 178 367 385 17
Cuts at: 3773 5225 5311 5323 5669 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 416 385 375 367 346 178
86 42 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2373 2508 2545 513
Size: 552 939 369 135 37 3642
Fragments arranged by size:
3642 939 552 369 135 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2038 2872 2890 2917 487
Size: 683 56 812 834 18 27 3244
Fragments arranged by size:
3244 834 812 683 56 27 18
BtsI GCAGTG_nn'
Cuts at: 3190 3984 4004 3190
Size: 794 20 4860
Fragments arranged by size:
4860 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2128 2511
Size: 54 1425 50 275 18 267 31 383
Cuts at: 2511 2694 2727 3121 4010 5639 8
Size: 183 33 394 889 1629 43
Fragments arranged by size:
1629 1425 889 394 383 275 267 183
54 50 43 33 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3604 1353
Size: 2251 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2405 2475 2747 2756 2879 3737 4334
Size: 393 773 70 272 9 123 858 597
Cuts at: 4334 5121 5389 1239
Size: 787 268 1524
Fragments arranged by size:
1524 858 787 773 597 393 272 268
123 70 9
EcoO109I rG'GnC_Cy
Cuts at: 2842 2843 2962 2842
Size: 1 119 5554
Fragments arranged by size:
5554 119 1
EcoRI G'AATT_C
Cuts at: 2983 3361 2983
Size: 378 5296
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 2868 4821 1678
Size: 1190 1953 2531
Fragments arranged by size:
2531 1953 1190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2268 2300 2654 2686 2783
Size: 32 135 32 696 32 354 32 97
Cuts at: 2783 2815 4315 4347 4389 4421 1373
Size: 32 1500 32 42 32 2626
Fragments arranged by size:
2626 1500 696 354 135 97 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2734 2785 3018 3264 669
Size: 988 281 796 51 233 246 3079
Fragments arranged by size:
3079 988 796 281 246 233 51
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5674
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KasI G'GCGC_C
Cuts at: 2864 2864
Size: 5674
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 3273 3273
Size: 5674
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2178 2299 4788 4972 1305
Size: 873 121 2489 184 2007
Fragments arranged by size:
2489 2007 873 184 121
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 10
Size: 54 1425 343 267 3585
Fragments arranged by size:
3585 1425 343 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 2147 2372 2378 3632 3991 4150
Size: 648 912 69 225 6 1254 359 159
Cuts at: 4150 518
Size: 2042
Fragments arranged by size:
2042 1254 912 648 359 225 159 69
6
MspA1I CmG'CkG
Cuts at: 1606 2395 2495 2521 2644 2723 3090 3767 4915
Size: 789 100 26 123 79 367 677 1148
Cuts at: 4915 5160 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 789 677 367 245 123 100
79 26
NaeI GCC'GGC
Cuts at: 2698 2698
Size: 5674
NarI GG'CG_CC
Cuts at: 2865 2865
Size: 5674
NcoI C'CATG_G
Cuts at: 513 1065 2004 2373 513
Size: 552 939 369 3814
Fragments arranged by size:
3814 939 552 369
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NgoMIV G'CCGG_C
Cuts at: 2696 2696
Size: 5674
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 2152 2200 3032 4577 1132
Size: 97 923 48 832 1545 2229
Fragments arranged by size:
2229 1545 923 832 97 48
PasI CC'CwG_GG
Cuts at: 1348 2443 2461 1348
Size: 1095 18 4561
Fragments arranged by size:
4561 1095 18
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5674
PfoI T'CCnGG_A
Cuts at: 2808 2808
Size: 5674
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2962 2962
Size: 5674
PshAI GACnn'nnGTC
Cuts at: 1613 2228 1613
Size: 615 5059
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PspOMI G'GGCC_C
Cuts at: 2842 2842
Size: 5674
PstI C_TGCA'G
Cuts at: 830 2115 2400 2961 3026 830
Size: 1285 285 561 65 3478
Fragments arranged by size:
3478 1285 561 285 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
PvuII CAG'CTG
Cuts at: 2395 2495 2521 2644 2395
Size: 100 26 123 5425
Fragments arranged by size:
5425 123 100 26
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SfcI C'TryA_G
Cuts at: 826 1045 2111 2396 2957 3022 4157 4838 5029
Size: 219 1066 285 561 65 1135 681 191
Cuts at: 5029 826
Size: 1471
Fragments arranged by size:
1471 1135 1066 681 561 285 219 191
65
SfoI GGC'GCC
Cuts at: 2866 2866
Size: 5674
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3778 4679 4941 820
Size: 197 926 1835 901 262 1553
Fragments arranged by size:
1835 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 3598 5
Size: 47 3546 2081
Fragments arranged by size:
3546 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5674
StyI C'CwwG_G
Cuts at: 513 1065 2004 2373 3113 513
Size: 552 939 369 740 3074
Fragments arranged by size:
3074 939 740 552 369
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2531 3701 3718 3871 4056 4501 325
Size: 1255 951 1170 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1170 951 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2139 2613 3920
Size: 274 80 33 51 1443 162 474 1307
Cuts at: 3920 5632 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1307 474 274 162 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2199 2231 5634 5666 2199
Size: 32 3403 32 2207
Fragments arranged by size:
3403 2207 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5674
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 3803 3803
Size: 5674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI