Restriction map for
pFN21AB4900
(Circular) (Six-base) MAPSORT of: pFN21AB4900.seq Check: 8153 from: 1 to: 6331
pFN21AB4900 6331 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4141 278
Size: 53 83 186 936 2605 2468
Fragments arranged by size:
2605 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3674 1937
Size: 1737 4594
Acc65I G'GTAC_C
Cuts at: 3652 3652
Size: 6331
AclI AA'CG_TT
Cuts at: 1563 4458 4831 1563
Size: 2895 373 3063
Fragments arranged by size:
3063 2895 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3530 4394 5778 6046 1632
Size: 1898 864 1384 268 1917
Fragments arranged by size:
1917 1898 1384 864 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6134
AflIII A'CryG_T
Cuts at: 1203 2532 5230 6207 1203
Size: 1329 2698 977 1327
Fragments arranged by size:
2698 1329 1327 977
AgeI A'CCGG_T
Cuts at: 5162 5162
Size: 6331
AhdI GACnn_n'nnGTC
Cuts at: 5060 5060
Size: 6331
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6331
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6331
AlwNI CAG_nnn'CTG
Cuts at: 2287 2714 2803 4074 5646 2287
Size: 427 89 1271 1572 2972
Fragments arranged by size:
2972 1572 1271 427 89
ApaLI G'TGCA_C
Cuts at: 4388 5544 4388
Size: 1156 5175
ApoI r'AATT_y
Cuts at: 1493 3330 3640 3866 4018 6127 1493
Size: 1837 310 226 152 2109 1697
Fragments arranged by size:
2109 1837 1697 310 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4885 160
Size: 872 3853 1606
Fragments arranged by size:
3853 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6331
AvaI C'yCGr_G
Cuts at: 1870 1943 3656 1870
Size: 73 1713 4545
Fragments arranged by size:
4545 1713 73
BamHI G'GATC_C
Cuts at: 3661 4028 3661
Size: 367 5964
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3652 5107 618
Size: 325 217 627 1865 1455 1842
Fragments arranged by size:
1865 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2031 3650 1391
Size: 640 1619 4072
Fragments arranged by size:
4072 1619 640
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2740 2925 928
Size: 971 841 185 4334
Fragments arranged by size:
4334 971 841 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4522 4556 1903
Size: 34 2585 34 3678
Fragments arranged by size:
3678 2585 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4223 5433 4223
Size: 1210 5121
BclI T'GATC_A
Cuts at: 1548 2523 1548
Size: 975 5356
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4942 136
Size: 107 122 71 631 605 3270 1525
Fragments arranged by size:
3270 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6326 6326
Size: 6331
BlpI GC'TnA_GC
Cuts at: 3748 3748
Size: 6331
Bme1580I G_kGCm'C
Cuts at: 1790 2456 2610 4392 5548 1790
Size: 666 154 1782 1156 2573
Fragments arranged by size:
2573 1782 1156 666 154
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6331
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2696 3467 4062
Size: 416 53 83 399 24 1279 771 595
Cuts at: 4062 5020 442
Size: 958 1753
Fragments arranged by size:
1753 1279 958 771 595 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2293 4991 1239
Size: 1054 2698 2579
Fragments arranged by size:
2698 2579 1054
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3231 4456 5321 5619 3231
Size: 1225 865 298 3943
Fragments arranged by size:
3943 1225 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4994 882
Size: 617 3495 2219
Fragments arranged by size:
3495 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4122 493
Size: 711 2918 2702
Fragments arranged by size:
2918 2702 711
BsaBI GATnn'nnATC
Cuts at: 6325 6325
Size: 6331
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4138 4520 5155 275
Size: 53 83 186 936 2605 382 635 1451
Fragments arranged by size:
2605 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3438 4764 5162 5436 5583 5994 3438
Size: 1326 398 274 147 411 3775
Fragments arranged by size:
3775 1326 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3479 3509 1178
Size: 30 165 30 2076 30 4000
Fragments arranged by size:
4000 2076 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2705 2999 1186
Size: 1519 294 4518
Fragments arranged by size:
4518 1519 294
BseYI C'CCAG_C
Cuts at: 2897 3476 5534 6030 2897
Size: 579 2058 496 3198
Fragments arranged by size:
3198 2058 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 1163
Size: 573 387 5371
Fragments arranged by size:
5371 573 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3781 4542 5570 1540
Size: 275 186 1780 761 1028 2301
Fragments arranged by size:
2301 1780 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3177 3492 4392 4477 5548 3177
Size: 315 900 85 1071 3960
Fragments arranged by size:
3960 1071 900 315 85
BsmI GAATG_Cn'
Cuts at: 2152 2370 3842 3935 2152
Size: 218 1472 93 4548
Fragments arranged by size:
4548 1472 218 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2456 2610 3177 3492 3650 4392
Size: 399 241 425 154 567 315 158 742
Cuts at: 4392 4477 5548 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 425 399 315 241
158 154 85
BspEI T'CCGG_A
Cuts at: 3438 5994 3438
Size: 2556 3775
BspHI T'CATG_A
Cuts at: 2838 4220 5950 2838
Size: 1382 1730 3219
Fragments arranged by size:
3219 1730 1382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3686 844
Size: 2842 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3080 4218 1394
Size: 429 1257 1138 3507
Fragments arranged by size:
3507 1257 1138 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2023 2400 3240 4826 5000 65
Size: 1183 775 377 840 1586 174 1396
Fragments arranged by size:
1586 1396 1183 840 775 377 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4975 5162 1700
Size: 110 3165 187 2869
Fragments arranged by size:
3165 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2952 96
Size: 2856 3475
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6331
BssSI C'ACGA_G
Cuts at: 4391 5403 4391
Size: 1012 5319
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6331
BstBI TT'CG_AA
Cuts at: 5135 5135
Size: 6331
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6331
BstXI CCAn_nnnn'nTGG
Cuts at: 3199 3457 3199
Size: 258 6073
BstYI r'GATC_y
Cuts at: 1972 2711 3661 4028 4413 4430 5882 5968 5980
Size: 739 950 367 385 17 1452 86 12
Cuts at: 5980 6326 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 950 739 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3336 3336
Size: 6331
BtgI C'CryG_G
Cuts at: 513 1065 2664 2874 513
Size: 552 1599 210 3970
Fragments arranged by size:
3970 1599 552 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3394 487
Size: 683 56 2168 3424
Fragments arranged by size:
3424 2168 683 56
BtsI GCAGTG_nn'
Cuts at: 3847 4641 4661 3847
Size: 794 20 5517
Fragments arranged by size:
5517 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6331
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2383 2418 529
Size: 35 1819 35 4442
Fragments arranged by size:
4442 1819 35 35
DraI TTT'AAA
Cuts at: 2566 2575 2602 3635 2566
Size: 9 27 1033 5262
Fragments arranged by size:
5262 1033 27 9
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3158 5338 809
Size: 507 1842 2180 1802
Fragments arranged by size:
2180 1842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 3778 4667
Size: 54 1425 50 275 18 258 1690 889
Cuts at: 4667 6296 8
Size: 1629 43
Fragments arranged by size:
1690 1629 1425 889 275 258 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3778 1537
Size: 275 1966 4090
Fragments arranged by size:
4090 1966 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2019 2585 2891 3097 3129 3543 4261 1353
Size: 666 566 306 206 32 414 718 3423
Fragments arranged by size:
3423 718 666 566 414 306 206 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4918 5292 5438 387
Size: 1064 138 3329 374 146 1280
Fragments arranged by size:
3329 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2293 3530 4394 4991 5778 6046 1239
Size: 393 661 1237 864 597 787 268 1524
Fragments arranged by size:
1524 1237 864 787 661 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3218 3218
Size: 6331
EcoO109I rG'GnC_Cy
Cuts at: 2800 3087 2800
Size: 287 6044
EcoRI G'AATT_C
Cuts at: 3330 3640 4018 3330
Size: 310 378 5643
Fragments arranged by size:
5643 378 310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 6331
FspI TGC'GCA
Cuts at: 4837 4837
Size: 6331
HaeII r_GCGC'y
Cuts at: 1678 2336 5478 1678
Size: 658 3142 2531
Fragments arranged by size:
3142 2531 658
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2190 2222 2361 2393 3159
Size: 32 135 32 618 32 139 32 766
Cuts at: 3159 3191 3479 3511 4972 5004 5046 5078 1373
Size: 32 288 32 1461 32 42 32 2626
Fragments arranged by size:
2626 1461 766 618 288 139 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3253 3675 3921 669
Size: 988 281 1315 422 246 3079
Fragments arranged by size:
3079 1315 988 422 281 246
HindIII A'AGCT_T
Cuts at: 748 2596 748
Size: 1848 4483
HpaI GTT'AAC
Cuts at: 3253 3921 3253
Size: 668 5663
KpnI G_GTAC'C
Cuts at: 3656 3656
Size: 6331
MfeI C'AATT_G
Cuts at: 3930 3930
Size: 6331
MluI A'CGCG_T
Cuts at: 6207 6207
Size: 6331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2233 5445 5629 1305
Size: 928 3212 184 2007
Fragments arranged by size:
3212 2007 928 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2090 10
Size: 54 1425 343 258 4251
Fragments arranged by size:
4251 1425 343 258 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2324 2405 2927 3197 3413 4289 4648
Size: 648 1158 81 522 270 216 876 359
Cuts at: 4648 4807 518
Size: 159 2042
Fragments arranged by size:
2042 1158 876 648 522 359 270 216
159 81
MspA1I CmG'CkG
Cuts at: 1606 2174 2501 3308 3326 3747 4424 5572 5817
Size: 568 327 807 18 421 677 1148 245
Cuts at: 5817 1606
Size: 2120
Fragments arranged by size:
2120 1148 807 677 568 421 327 245
18
NcoI C'CATG_G
Cuts at: 513 1065 2664 513
Size: 552 1599 4180
Fragments arranged by size:
4180 1599 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6331
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6331
NotI GC'GGCC_GC
Cuts at: 3778 3778
Size: 6331
NspI r_CATG'y
Cuts at: 1132 1229 2470 2536 3689 5234 1132
Size: 97 1241 66 1153 1545 2229
Fragments arranged by size:
2229 1545 1241 1153 97 66
PasI CC'CwG_GG
Cuts at: 1348 2734 3469 1348
Size: 1386 735 4210
Fragments arranged by size:
4210 1386 735
PciI A'CATG_T
Cuts at: 2532 5230 2532
Size: 2698 3633
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6331
PfoI T'CCnGG_A
Cuts at: 2514 2707 3426 2514
Size: 193 719 5419
Fragments arranged by size:
5419 719 193
PmeI GTTT'AAAC
Cuts at: 3635 3635
Size: 6331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4400 4432 5913 5945 4400
Size: 32 1481 32 4786
Fragments arranged by size:
4786 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3087 3087
Size: 6331
PshAI GACnn'nnGTC
Cuts at: 1613 3374 1613
Size: 1761 4570
PsiI TTA'TAA
Cuts at: 3901 3901
Size: 6331
PstI C_TGCA'G
Cuts at: 830 2506 3683 830
Size: 1676 1177 3478
Fragments arranged by size:
3478 1676 1177
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6331
PvuII CAG'CTG
Cuts at: 2501 3308 3326 2501
Size: 807 18 5506
Fragments arranged by size:
5506 807 18
SalI G'TCGA_C
Cuts at: 1936 3673 1936
Size: 1737 4594
SapI GCTCTTCn'nnn_
Cuts at: 2019 2019
Size: 6331
SbfI CC_TGCA'GG
Cuts at: 3683 3683
Size: 6331
ScaI AGT'ACT
Cuts at: 2243 4579 6291 2243
Size: 2336 1712 2283
Fragments arranged by size:
2336 2283 1712
SfcI C'TryA_G
Cuts at: 826 1045 2373 2502 3679 4814 5495 5686 826
Size: 219 1328 129 1177 1135 681 191 1471
Fragments arranged by size:
1471 1328 1177 1135 681 219 191 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6331
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6331
SmaI CCC'GGG
Cuts at: 1872 3658 1872
Size: 1786 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3246 4435 5336 5598 820
Size: 197 926 1303 1189 901 262 1553
Fragments arranged by size:
1553 1303 1189 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4122 493
Size: 3629 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6331
SphI G_CATG'C
Cuts at: 1132 3689 1132
Size: 2557 3774
SspI AAT'ATT
Cuts at: 5 52 4255 5
Size: 47 4203 2081
Fragments arranged by size:
4203 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6331
StyI C'CwwG_G
Cuts at: 513 1065 2194 2664 3038 3770 513
Size: 552 1129 470 374 732 3074
Fragments arranged by size:
3074 1129 732 552 470 374
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2624 2915 4358 4375 4528 4713 5158
Size: 1255 1044 291 1443 17 153 185 445
Cuts at: 5158 325
Size: 1498
Fragments arranged by size:
1498 1443 1255 1044 445 291 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2241 2952 4577
Size: 274 80 33 51 1443 264 711 1625
Cuts at: 4577 6289 96
Size: 1712 138
Fragments arranged by size:
1712 1625 1443 711 274 264 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6291 6323 6291
Size: 32 6299
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6331
XbaI T'CTAG_A
Cuts at: 3667 3667
Size: 6331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2404 2842 3196 3454 2404
Size: 438 354 258 5281
Fragments arranged by size:
5281 438 354 258
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6331
XmaI C'CCGG_G
Cuts at: 1870 3656 1870
Size: 1786 4545
XmnI GAAnn'nnTTC
Cuts at: 2534 4460 2534
Size: 1926 4405
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4139 276
Size: 53 83 186 936 2605 2468
Fragments arranged by size:
2605 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI