Restriction map for
pFN21AB4899
(Circular) (Six-base) MAPSORT of: pFN21AB4899.seq Check: 710 from: 1 to: 6112
pFN21AB4899 6112 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3305 3922 278
Size: 53 83 186 936 1769 617 2468
Fragments arranged by size:
2468 1769 936 617 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3455 1937
Size: 1518 4594
Acc65I G'GTAC_C
Cuts at: 3433 3433
Size: 6112
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2248 4175 5559 5827 1632
Size: 616 1927 1384 268 1917
Fragments arranged by size:
1927 1917 1384 616 268
AfeI AGC'GCT
Cuts at: 3314 3314
Size: 6112
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 3318 5011 5988 1203
Size: 2115 1693 977 1327
Fragments arranged by size:
2115 1693 1327 977
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6112
AlwNI CAG_nnn'CTG
Cuts at: 2419 3855 5427 2419
Size: 1436 1572 3104
Fragments arranged by size:
3104 1572 1436
ApaLI G'TGCA_C
Cuts at: 4169 5325 4169
Size: 1156 4956
ApoI r'AATT_y
Cuts at: 1493 2545 2794 3421 3647 3799 5908 1493
Size: 1052 249 627 226 152 2109 1697
Fragments arranged by size:
2109 1697 1052 627 249 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 3365 3437 1870
Size: 73 1422 72 4545
Fragments arranged by size:
4545 1422 73 72
BamHI G'GATC_C
Cuts at: 3442 3809 3442
Size: 367 5745
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2289 2455 2611 3433 4888
Size: 325 217 627 502 166 156 822 1455
Cuts at: 4888 618
Size: 1842
Fragments arranged by size:
1842 1455 822 627 502 325 217 166
156
BanII G_rGCy'C
Cuts at: 1391 2870 3431 1391
Size: 1479 561 4072
Fragments arranged by size:
4072 1479 561
BbeI G_GCGC'C
Cuts at: 2293 2615 2293
Size: 322 5790
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4303 4337 1903
Size: 34 2366 34 3678
Fragments arranged by size:
3678 2366 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4004 5214 4004
Size: 1210 4902
BclI T'GATC_A
Cuts at: 1548 2502 1548
Size: 954 5158
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 4723 136
Size: 107 122 71 631 605 376 2675 1525
Fragments arranged by size:
2675 1525 631 605 376 122 107 71
BglII A'GATC_T
Cuts at: 6107 6107
Size: 6112
BlpI GC'TnA_GC
Cuts at: 2735 3254 3529 2735
Size: 519 275 5318
Fragments arranged by size:
5318 519 275
Bme1580I G_kGCm'C
Cuts at: 1790 4173 5329 1790
Size: 2383 1156 2573
Fragments arranged by size:
2573 2383 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6112
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3843 4801 442
Size: 416 53 83 399 24 2426 958 1753
Fragments arranged by size:
2426 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2087 2087
Size: 6112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 2513 2867 3134 3180 3326 4772 1239
Size: 1229 45 354 267 46 146 1446 2579
Fragments arranged by size:
2579 1446 1229 354 267 146 46 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2057 4237 5102 5400 2057
Size: 2180 865 298 2769
Fragments arranged by size:
2769 2180 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4775 882
Size: 617 3276 2219
Fragments arranged by size:
3276 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3319 3903 493
Size: 711 2115 584 2702
Fragments arranged by size:
2702 2115 711 584
BsaBI GATnn'nnATC
Cuts at: 6106 6106
Size: 6112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2290 2612 2819 3302
Size: 53 83 186 936 757 322 207 483
Cuts at: 3302 3390 3919 4301 4936 275
Size: 88 529 382 635 1451
Fragments arranged by size:
1451 936 757 635 529 483 382 322
207 186 88 83 53
BsaWI w'CCGG_w
Cuts at: 4545 4943 5217 5364 5775 4545
Size: 398 274 147 411 4882
Fragments arranged by size:
4882 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 2819 2849 1178
Size: 30 165 30 1140 30 246 30 4441
Fragments arranged by size:
4441 1140 246 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2870 3031 1186
Size: 1684 161 4267
Fragments arranged by size:
4267 1684 161
BseYI C'CCAG_C
Cuts at: 2684 3352 5315 5811 2684
Size: 668 1963 496 2985
Fragments arranged by size:
2985 1963 668 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2245 3359 1163
Size: 573 509 1114 3916
Fragments arranged by size:
3916 1114 573 509
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3562 4323 5351 1540
Size: 275 186 1561 761 1028 2301
Fragments arranged by size:
2301 1561 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2870 4173 4258 5329 2870
Size: 1303 85 1071 3653
Fragments arranged by size:
3653 1303 1071 85
BsmI GAATG_Cn'
Cuts at: 2298 2628 3623 3716 2298
Size: 330 995 93 4694
Fragments arranged by size:
4694 995 330 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3156 3156
Size: 6112
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2870 3431 4173 4258 5329 1391
Size: 399 1080 561 742 85 1071 2174
Fragments arranged by size:
2174 1080 1071 742 561 399 85
BspEI T'CCGG_A
Cuts at: 5775 5775
Size: 6112
BspHI T'CATG_A
Cuts at: 2185 4001 5731 2185
Size: 1816 1730 2566
Fragments arranged by size:
2566 1816 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3061 3467 844
Size: 2217 406 3489
Fragments arranged by size:
3489 2217 406
BsrBI CCG'CTC
Cuts at: 1394 1823 3999 1394
Size: 429 2176 3507
Fragments arranged by size:
3507 2176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4607 4781 65
Size: 1183 3359 174 1396
Fragments arranged by size:
3359 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3068 3207 4756 4943 1700
Size: 110 1258 139 1549 187 2869
Fragments arranged by size:
2869 1549 1258 187 139 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 4172 5184 4172
Size: 1012 5100
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2149 2158 794
Size: 1355 9 4748
Fragments arranged by size:
4748 1355 9
BstBI TT'CG_AA
Cuts at: 2946 4916 2946
Size: 1970 4142
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6112
BstYI r'GATC_y
Cuts at: 1972 3442 3809 4194 4211 5663 5749 5761 6107
Size: 1470 367 385 17 1452 86 12 346
Cuts at: 6107 1972
Size: 1977
Fragments arranged by size:
1977 1470 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5560
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5373
Fragments arranged by size:
5373 683 56
BtsI GCAGTG_nn'
Cuts at: 2147 3628 4422 4442 2147
Size: 1481 794 20 3817
Fragments arranged by size:
3817 1481 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6077
DraI TTT'AAA
Cuts at: 3416 3416
Size: 6112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5119 809
Size: 507 3803 1802
Fragments arranged by size:
3803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2410 3066 3559
Size: 54 1425 50 275 18 580 656 493
Cuts at: 3559 4448 6077 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 656 580 493 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3559 1537
Size: 275 1747 4090
Fragments arranged by size:
4090 1747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 4042 1353
Size: 780 1909 3423
Fragments arranged by size:
3423 1909 780
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2182 4699 5073 5219 387
Size: 1064 138 593 2517 374 146 1280
Fragments arranged by size:
2517 1280 1064 593 374 146 138
EcoICRI GAG'CTC
Cuts at: 2868 2868
Size: 6112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2248 2468 2513 2867 3134 3180 3326
Size: 393 616 220 45 354 267 46 146
Cuts at: 3326 4175 4772 5559 5827 1239
Size: 849 597 787 268 1524
Fragments arranged by size:
1524 849 787 616 597 393 354 268
267 220 146 46 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2432 2432
Size: 6112
EcoO109I rG'GnC_Cy
Cuts at: 2106 2106
Size: 6112
EcoRI G'AATT_C
Cuts at: 3421 3799 3421
Size: 378 5734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 6112
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 2080 2293 2615 3316 5259 1678
Size: 402 213 322 701 1943 2531
Fragments arranged by size:
2531 1943 701 402 322 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2515 2547 2819 2851 3107
Size: 32 135 32 943 32 272 32 256
Cuts at: 3107 3139 4753 4785 4827 4859 1373
Size: 32 1614 32 42 32 2626
Fragments arranged by size:
2626 1614 943 272 256 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2217 3456 3702 669
Size: 988 281 279 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 279 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6112
HpaI GTT'AAC
Cuts at: 2217 3702 2217
Size: 1485 4627
KasI G'GCGC_C
Cuts at: 2289 2611 2289
Size: 322 5790
KpnI G_GTAC'C
Cuts at: 3437 3437
Size: 6112
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5226 5410 1305
Size: 3921 184 2007
Fragments arranged by size:
3921 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2412 10
Size: 54 1425 343 580 3710
Fragments arranged by size:
3710 1425 580 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4070 4429 4588 518
Size: 648 2904 359 159 2042
Fragments arranged by size:
2904 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2287 3059 3097 3153 3528 4205 5353 5598
Size: 681 772 38 56 375 677 1148 245
Cuts at: 5598 1606
Size: 2120
Fragments arranged by size:
2120 1148 772 681 677 375 245 56
38
NaeI GCC'GGC
Cuts at: 3070 3070
Size: 6112
NarI GG'CG_CC
Cuts at: 2290 2612 2290
Size: 322 5790
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5560
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NgoMIV G'CCGG_C
Cuts at: 3068 3068
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6112
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 2668 3470 5015 1132
Size: 97 1439 802 1545 2229
Fragments arranged by size:
2229 1545 1439 802 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6112
PciI A'CATG_T
Cuts at: 5011 5011
Size: 6112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6112
PfoI T'CCnGG_A
Cuts at: 2655 2888 3111 2655
Size: 233 223 5656
Fragments arranged by size:
5656 233 223
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2106 2106
Size: 6112
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6112
PsiI TTA'TAA
Cuts at: 3682 3682
Size: 6112
PstI C_TGCA'G
Cuts at: 830 2394 2960 3058 3464 830
Size: 1564 566 98 406 3478
Fragments arranged by size:
3478 1564 566 406 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
PvuII CAG'CTG
Cuts at: 3059 3097 3153 3059
Size: 38 56 6018
Fragments arranged by size:
6018 56 38
SacI G_AGCT'C
Cuts at: 2870 2870
Size: 6112
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SanDI GG'GwC_CC
Cuts at: 2106 2106
Size: 6112
SapI GCTCTTCn'nnn_
Cuts at: 2133 2133
Size: 6112
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SexAI A'CCwGG_T
Cuts at: 2034 2034
Size: 6112
SfcI C'TryA_G
Cuts at: 826 1045 2390 2956 3054 3460 4595 5276 5467
Size: 219 1345 566 98 406 1135 681 191
Cuts at: 5467 826
Size: 1471
Fragments arranged by size:
1471 1345 1135 681 566 406 219 191
98
SfoI GGC'GCC
Cuts at: 2291 2613 2291
Size: 322 5790
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 3207 1700
Size: 1507 4605
SmaI CCC'GGG
Cuts at: 1872 3439 1872
Size: 1567 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2072 4216 5117 5379 820
Size: 197 926 129 2144 901 262 1553
Fragments arranged by size:
2144 1553 926 901 262 197 129
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 3470 1132
Size: 2338 3774
SspI AAT'ATT
Cuts at: 5 52 4036 5
Size: 47 3984 2081
Fragments arranged by size:
3984 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6112
StyI C'CwwG_G
Cuts at: 513 1065 2428 3551 513
Size: 552 1363 1123 3074
Fragments arranged by size:
3074 1363 1123 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2459 2698 4139 4156 4309 4494 4939
Size: 1255 879 239 1441 17 153 185 445
Cuts at: 4939 325
Size: 1498
Fragments arranged by size:
1498 1441 1255 879 445 239 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4358 6070 96
Size: 274 80 33 51 1443 2381 1712 138
Fragments arranged by size:
2381 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2548 2575 2580 2651 2683 6072 6104 2543
Size: 5 27 5 71 32 3389 32 2551
Fragments arranged by size:
3389 2551 71 32 32 27 5 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6112
XbaI T'CTAG_A
Cuts at: 3448 3448
Size: 6112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2310 2310
Size: 6112
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 3437 1870
Size: 1567 4545
XmnI GAAnn'nnTTC
Cuts at: 2012 2442 4241 2012
Size: 430 1799 3883
Fragments arranged by size:
3883 1799 430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3303 3920 276
Size: 53 83 186 936 1769 617 2468
Fragments arranged by size:
2468 1769 936 617 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SfiI SrfI SwaI