Restriction map for
pFN21AB4895
(Circular) (Six-base) MAPSORT of: pFN21AB4895.seq Check: 1657 from: 1 to: 6052
pFN21AB4895 6052 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3862 278
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3395 1937
Size: 1458 4594
Acc65I G'GTAC_C
Cuts at: 3373 3373
Size: 6052
AclI AA'CG_TT
Cuts at: 1563 4179 4552 1563
Size: 2616 373 3063
Fragments arranged by size:
3063 2616 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2214 2592 4115 5499 5767 1632
Size: 582 378 1523 1384 268 1917
Fragments arranged by size:
1917 1523 1384 582 378 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5855
AflIII A'CryG_T
Cuts at: 1203 4951 5928 1203
Size: 3748 977 1327
Fragments arranged by size:
3748 1327 977
AgeI A'CCGG_T
Cuts at: 2987 4883 2987
Size: 1896 4156
AhdI GACnn_n'nnGTC
Cuts at: 3337 4781 3337
Size: 1444 4608
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6052
AlwNI CAG_nnn'CTG
Cuts at: 3250 3795 5367 3250
Size: 545 1572 3935
Fragments arranged by size:
3935 1572 545
ApaLI G'TGCA_C
Cuts at: 4109 5265 4109
Size: 1156 4896
ApoI r'AATT_y
Cuts at: 1493 3005 3088 3148 3361 3587 3739 5848 1493
Size: 1512 83 60 213 226 152 2109 1697
Fragments arranged by size:
2109 1697 1512 226 213 152 83 60
AseI AT'TA_AT
Cuts at: 160 1032 4606 160
Size: 872 3574 1606
Fragments arranged by size:
3574 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
AvaI C'yCGr_G
Cuts at: 1870 1943 3377 1870
Size: 73 1434 4545
Fragments arranged by size:
4545 1434 73
BamHI G'GATC_C
Cuts at: 3382 3749 3382
Size: 367 5685
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2705 3373 4828 618
Size: 325 217 627 918 668 1455 1842
Fragments arranged by size:
1842 1455 918 668 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3081 3371 1391
Size: 1690 290 4072
Fragments arranged by size:
4072 1690 290
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5081
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4243 4277 1903
Size: 34 2306 34 3678
Fragments arranged by size:
3678 2306 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3944 5154 3944
Size: 1210 4842
BclI T'GATC_A
Cuts at: 1548 2821 1548
Size: 1273 4779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4663 136
Size: 107 122 71 631 605 2991 1525
Fragments arranged by size:
2991 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2550 6047 2550
Size: 3497 2555
BlpI GC'TnA_GC
Cuts at: 2012 2071 3469 2012
Size: 59 1398 4595
Fragments arranged by size:
4595 1398 59
Bme1580I G_kGCm'C
Cuts at: 1790 2538 2871 4113 5269 1790
Size: 748 333 1242 1156 2573
Fragments arranged by size:
2573 1242 1156 748 333
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6052
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3783 4741 442
Size: 416 53 83 399 24 2366 958 1753
Fragments arranged by size:
2366 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3243 1056
Size: 2187 3865
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3310 4712 1239
Size: 2071 1402 2579
Fragments arranged by size:
2579 2071 1402
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4177 5042 5340 4177
Size: 865 298 4889
Fragments arranged by size:
4889 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2784 4715 882
Size: 617 1285 1931 2219
Fragments arranged by size:
2219 1931 1285 617
BsaAI yAC'GTr
Cuts at: 493 1204 3843 493
Size: 711 2639 2702
Fragments arranged by size:
2702 2639 711
BsaBI GATnn'nnATC
Cuts at: 6046 6046
Size: 6052
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3859 4241 4876 275
Size: 53 83 186 936 2326 382 635 1451
Fragments arranged by size:
2326 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2987 4485 4883 5157 5304 5715 2987
Size: 1498 398 274 147 411 3324
Fragments arranged by size:
3324 1498 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5827
Fragments arranged by size:
5827 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2691 1186
Size: 1505 4547
BseYI C'CCAG_C
Cuts at: 2681 5255 5751 2681
Size: 2574 496 2982
Fragments arranged by size:
2982 2574 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2495 1163
Size: 573 759 4720
Fragments arranged by size:
4720 759 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3502 4263 5291 1540
Size: 275 186 1501 761 1028 2301
Fragments arranged by size:
2301 1501 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2018 2861 3081 4113 4198 5269 2018
Size: 843 220 1032 85 1071 2801
Fragments arranged by size:
2801 1071 1032 843 220 85
BsmI GAATG_Cn'
Cuts at: 3563 3656 3563
Size: 93 5959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2538 2861 2871 3081 3371 4113
Size: 399 228 520 323 10 210 290 742
Cuts at: 4113 4198 5269 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 520 399 323 290 228
210 85 10
BspEI T'CCGG_A
Cuts at: 5715 5715
Size: 6052
BspHI T'CATG_A
Cuts at: 3016 3941 5671 3016
Size: 925 1730 3397
Fragments arranged by size:
3397 1730 925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3407 844
Size: 2563 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3939 1394
Size: 429 2116 3507
Fragments arranged by size:
3507 2116 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4547 4721 65
Size: 1183 3299 174 1396
Fragments arranged by size:
3299 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2987 4696 4883 1700
Size: 110 1177 1709 187 2869
Fragments arranged by size:
2869 1709 1177 187 110
BsrGI T'GTAC_A
Cuts at: 96 2875 96
Size: 2779 3273
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6052
BssSI C'ACGA_G
Cuts at: 4112 5124 4112
Size: 1012 5040
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6052
BstBI TT'CG_AA
Cuts at: 2578 4856 2578
Size: 2278 3774
BstEII G'GTnAC_C
Cuts at: 1184 2889 1184
Size: 1705 4347
BstYI r'GATC_y
Cuts at: 1972 2109 2550 3382 3749 4134 4151 5603 5689
Size: 137 441 832 367 385 17 1452 86
Cuts at: 5689 5701 6047 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 832 441 385 367 346 137
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2235 513
Size: 552 1170 4330
Fragments arranged by size:
4330 1170 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5313
Fragments arranged by size:
5313 683 56
BtsI GCAGTG_nn'
Cuts at: 2429 3568 4362 4382 2429
Size: 1139 794 20 4099
Fragments arranged by size:
4099 1139 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6017
DraI TTT'AAA
Cuts at: 3356 3356
Size: 6052
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5059 809
Size: 507 3743 1802
Fragments arranged by size:
3743 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2621 3499 4388
Size: 54 1425 50 275 18 791 878 889
Cuts at: 4388 6017 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 878 791 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3499 1537
Size: 275 1687 4090
Fragments arranged by size:
4090 1687 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 2373 2782 3086 3982 1353
Size: 837 183 409 304 896 3423
Fragments arranged by size:
3423 896 837 409 304 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4639 5013 5159 387
Size: 1064 138 3050 374 146 1280
Fragments arranged by size:
3050 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3079 3079
Size: 6052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 2592 3310 4115 4712 5499 5767
Size: 393 582 378 718 805 597 787 268
Cuts at: 5767 1239
Size: 1524
Fragments arranged by size:
1524 805 787 718 597 582 393 378
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2635 2635
Size: 6052
EcoO109I rG'GnC_Cy
Cuts at: 2053 2627 3233 2053
Size: 574 606 4872
Fragments arranged by size:
4872 606 574
EcoRI G'AATT_C
Cuts at: 3148 3361 3739 3148
Size: 213 378 5461
Fragments arranged by size:
5461 378 213
FspI TGC'GCA
Cuts at: 4558 4558
Size: 6052
HaeII r_GCGC'y
Cuts at: 1678 3177 5199 1678
Size: 1499 2022 2531
Fragments arranged by size:
2531 2022 1499
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2828 2860 3323 3355 4693
Size: 32 135 32 1256 32 463 32 1338
Cuts at: 4693 4725 4767 4799 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1338 1256 463 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3396 3642 669
Size: 988 281 1458 246 3079
Fragments arranged by size:
3079 1458 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6052
HpaI GTT'AAC
Cuts at: 3642 3642
Size: 6052
KpnI G_GTAC'C
Cuts at: 3377 3377
Size: 6052
MfeI C'AATT_G
Cuts at: 3651 3651
Size: 6052
MluI A'CGCG_T
Cuts at: 5928 5928
Size: 6052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2385 3237 5166 5350 1305
Size: 1080 852 1929 184 2007
Fragments arranged by size:
2007 1929 1080 852 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4230
Fragments arranged by size:
4230 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2612 3021 3069 3129 4010 4369 4528
Size: 648 1446 409 48 60 881 359 159
Cuts at: 4528 518
Size: 2042
Fragments arranged by size:
2042 1446 881 648 409 359 159 60
48
MspA1I CmG'CkG
Cuts at: 1606 2620 3250 3468 4145 5293 5538 1606
Size: 1014 630 218 677 1148 245 2120
Fragments arranged by size:
2120 1148 1014 677 630 245 218
NcoI C'CATG_G
Cuts at: 513 1065 2235 513
Size: 552 1170 4330
Fragments arranged by size:
4330 1170 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6052
NheI G'CTAG_C
Cuts at: 1052 3239 1052
Size: 2187 3865
NotI GC'GGCC_GC
Cuts at: 3499 3499
Size: 6052
NspI r_CATG'y
Cuts at: 1132 1229 3410 4955 1132
Size: 97 2181 1545 2229
Fragments arranged by size:
2229 2181 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6052
PciI A'CATG_T
Cuts at: 4951 4951
Size: 6052
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3193 1108
Size: 2085 3967
PmeI GTTT'AAAC
Cuts at: 3356 3356
Size: 6052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2559 2591 4121 4153 5634 5666 2559
Size: 32 1530 32 1481 32 2945
Fragments arranged by size:
2945 1530 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2627 3233 2627
Size: 606 5446
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6052
PsiI TTA'TAA
Cuts at: 2922 3622 2922
Size: 700 5352
PstI C_TGCA'G
Cuts at: 830 3100 3318 3404 830
Size: 2270 218 86 3478
Fragments arranged by size:
3478 2270 218 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6052
PvuII CAG'CTG
Cuts at: 3250 3250
Size: 6052
SacI G_AGCT'C
Cuts at: 3081 3081
Size: 6052
SalI G'TCGA_C
Cuts at: 1936 3394 1936
Size: 1458 4594
SapI GCTCTTCn'nnn_
Cuts at: 3086 3086
Size: 6052
SbfI CC_TGCA'GG
Cuts at: 3404 3404
Size: 6052
ScaI AGT'ACT
Cuts at: 4300 6012 4300
Size: 1712 4340
SfcI C'TryA_G
Cuts at: 826 1045 2368 3096 3153 3314 3400 4535 5216
Size: 219 1323 728 57 161 86 1135 681
Cuts at: 5216 5407 826
Size: 191 1471
Fragments arranged by size:
1471 1323 1135 728 681 219 191 161
86 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6052
SmaI CCC'GGG
Cuts at: 1872 3379 1872
Size: 1507 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4156 5057 5319 820
Size: 197 926 2213 901 262 1553
Fragments arranged by size:
2213 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3843 493
Size: 3350 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6052
SphI G_CATG'C
Cuts at: 1132 3410 1132
Size: 2278 3774
SspI AAT'ATT
Cuts at: 5 52 3976 5
Size: 47 3924 2081
Fragments arranged by size:
3924 2081 47
StuI AGG'CCT
Cuts at: 1517 2507 1517
Size: 990 5062
StyI C'CwwG_G
Cuts at: 513 1065 2235 3491 513
Size: 552 1170 1256 3074
Fragments arranged by size:
3074 1256 1170 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2278 4079 4096 4249 4434 4879 325
Size: 1255 698 1801 17 153 185 445 1498
Fragments arranged by size:
1801 1498 1255 698 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2875 2953 4298
Size: 274 80 33 51 1443 898 78 1345
Cuts at: 4298 6010 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1345 898 274 138 80 78
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2686 2718 6012 6044 2686
Size: 32 3294 32 2694
Fragments arranged by size:
3294 2694 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6052
XbaI T'CTAG_A
Cuts at: 3388 3388
Size: 6052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3224 3251 3224
Size: 27 6025
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6052
XmaI C'CCGG_G
Cuts at: 1870 3377 1870
Size: 1507 4545
XmnI GAAnn'nnTTC
Cuts at: 4181 4181
Size: 6052
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3860 276
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI