Restriction map for
pFN21AB4894
(Circular) (Six-base) MAPSORT of: pFN21AB4894.seq Check: 5080 from: 1 to: 5926
pFN21AB4894 5926 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 1937
Size: 1332 4594
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3989 5373 5641 1632
Size: 2357 1384 268 1917
Fragments arranged by size:
2357 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 2236 2639 2833 4825 5802 1203
Size: 1033 403 194 1992 977 1327
Fragments arranged by size:
1992 1327 1033 977 403 194
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 2379 1166
Size: 1213 4713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5926
AlwNI CAG_nnn'CTG
Cuts at: 3669 5241 3669
Size: 1572 4354
ApaI G_GGCC'C
Cuts at: 2753 2753
Size: 5926
ApaLI G'TGCA_C
Cuts at: 2174 3983 5139 2174
Size: 1809 1156 2961
Fragments arranged by size:
2961 1809 1156
ApoI r'AATT_y
Cuts at: 1493 3235 3461 3613 5722 1493
Size: 1742 226 152 2109 1697
Fragments arranged by size:
2109 1742 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2203 2511 2655 2691 2753 3251 1870
Size: 73 260 308 144 36 62 498 4545
Fragments arranged by size:
4545 498 308 260 144 73 62 36
BamHI G'GATC_C
Cuts at: 2790 3178 3256 3623 2790
Size: 388 78 367 5093
Fragments arranged by size:
5093 388 367 78
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2272 2310 2494 2696
Size: 325 217 627 351 134 38 184 202
Cuts at: 2696 2912 3247 4702 618
Size: 216 335 1455 1842
Fragments arranged by size:
1842 1455 627 351 335 325 217 216
202 184 134 38
BanII G_rGCy'C
Cuts at: 1391 2070 2204 2512 2753 3077 3245 1391
Size: 679 134 308 241 324 168 4072
Fragments arranged by size:
4072 679 324 308 241 168 134
BbeI G_GCGC'C
Cuts at: 2276 2314 2700 2276
Size: 38 386 5502
Fragments arranged by size:
5502 386 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4955
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4117 4151 1903
Size: 34 50 34 2096 34 3678
Fragments arranged by size:
3678 2096 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3818 5028 3818
Size: 1210 4716
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2308 2492 2736
Size: 107 122 71 631 605 636 184 244
Cuts at: 2736 3007 3114 4537 136
Size: 271 107 1423 1525
Fragments arranged by size:
1525 1423 636 631 605 271 244 184
122 107 107 71
BglII A'GATC_T
Cuts at: 5921 5921
Size: 5926
BlpI GC'TnA_GC
Cuts at: 3343 3343
Size: 5926
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2178 2753 2785 3987 5143 1790
Size: 353 35 575 32 1202 1156 2573
Fragments arranged by size:
2573 1202 1156 575 353 35 32
BmgBI CAC'GTC
Cuts at: 1318 2642 1318
Size: 1324 4602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2792 3657 4615
Size: 416 53 83 399 24 1375 865 958
Cuts at: 4615 442
Size: 1753
Fragments arranged by size:
1753 1375 958 865 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2912 2932 3004 4586 1239
Size: 893 780 20 72 1582 2579
Fragments arranged by size:
2579 1582 893 780 72 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2484 2834 4051 4916 5214 2484
Size: 350 1217 865 298 3196
Fragments arranged by size:
3196 1217 865 350 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2282 4589 882
Size: 617 783 2307 2219
Fragments arranged by size:
2307 2219 783 617
BsaAI yAC'GTr
Cuts at: 493 1204 2173 2836 3717 493
Size: 711 969 663 881 2702
Fragments arranged by size:
2702 969 881 711 663
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 2273 2311 2697
Size: 53 83 186 936 528 212 38 386
Cuts at: 2697 3733 4115 4750 275
Size: 1036 382 635 1451
Fragments arranged by size:
1451 1036 936 635 528 386 382 212
186 83 53 38
BsaWI w'CCGG_w
Cuts at: 4359 4757 5031 5178 5589 4359
Size: 398 274 147 411 4696
Fragments arranged by size:
4696 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2287 2317 2618 2648 1178
Size: 30 165 30 884 30 301 30 4456
Fragments arranged by size:
4456 884 301 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3009 3068 3188 1186
Size: 1823 59 120 3924
Fragments arranged by size:
3924 1823 120 59
BseYI C'CCAG_C
Cuts at: 2425 2989 5129 5625 2425
Size: 564 2140 496 2726
Fragments arranged by size:
2726 2140 564 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2057 2393 2690 2801 1163
Size: 573 321 336 297 111 4288
Fragments arranged by size:
4288 573 336 321 297 111
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2055 2578 3376 4137 5165 1540
Size: 275 186 54 523 798 761 1028 2301
Fragments arranged by size:
2301 1028 798 761 523 275 186 54
BsiHKAI G_wGCw'C
Cuts at: 2178 2210 3987 4072 5143 2178
Size: 32 1777 85 1071 2961
Fragments arranged by size:
2961 1777 1071 85 32
BsmI GAATG_Cn'
Cuts at: 3437 3530 3437
Size: 93 5833
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2143 2178 2204 2210 2512 2753
Size: 399 280 73 35 26 6 302 241
Cuts at: 2753 2785 3077 3245 3987 4072 5143 1391
Size: 32 292 168 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 302 292 280 241
168 85 73 35 32 26 6
BspEI T'CCGG_A
Cuts at: 5589 5589
Size: 5926
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3281 844
Size: 2437 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3007 3813 1394
Size: 429 1184 806 3507
Fragments arranged by size:
3507 1184 806 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 4595 65
Size: 1183 3173 174 1396
Fragments arranged by size:
3173 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2412 2743 4570 4757 1700
Size: 110 602 331 1827 187 2869
Fragments arranged by size:
2869 1827 602 331 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5926
BssHII G'CGCG_C
Cuts at: 1925 2152 2154 1925
Size: 227 2 5697
Fragments arranged by size:
5697 227 2
BssSI C'ACGA_G
Cuts at: 3986 4998 3986
Size: 1012 4914
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3222 794
Size: 2428 3498
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstXI CCAn_nnnn'nTGG
Cuts at: 2269 2269
Size: 5926
BstYI r'GATC_y
Cuts at: 1972 2790 3178 3256 3623 4008 4025 5477 5563
Size: 818 388 78 367 385 17 1452 86
Cuts at: 5563 5575 5921 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 818 388 385 367 346 86
78 17 12
Bsu36I CC'TnA_GG
Cuts at: 2144 3012 2144
Size: 868 5058
BtgI C'CryG_G
Cuts at: 513 1065 2055 2091 2545 2664 513
Size: 552 990 36 454 119 3775
Fragments arranged by size:
3775 990 552 454 119 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5187
Fragments arranged by size:
5187 683 56
BtsI GCAGTG_nn'
Cuts at: 2724 2783 2818 3442 4236 4256 2724
Size: 59 35 624 794 20 4394
Fragments arranged by size:
4394 794 624 59 35 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4933 809
Size: 507 3617 1802
Fragments arranged by size:
3617 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2083 2565 2724
Size: 54 1425 50 275 18 253 482 159
Cuts at: 2724 3373 4262 5891 8
Size: 649 889 1629 43
Fragments arranged by size:
1629 1425 889 649 482 275 253 159
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3373 1537
Size: 275 1561 4090
Fragments arranged by size:
4090 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3856 1353
Size: 2503 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2912 2932 3004 3989 4586 5373
Size: 393 500 780 20 72 985 597 787
Cuts at: 5373 5641 1239
Size: 268 1524
Fragments arranged by size:
1524 985 787 780 597 500 393 268
72 20
EcoO109I rG'GnC_Cy
Cuts at: 2422 2750 2804 2994 3094 2422
Size: 328 54 190 100 5254
Fragments arranged by size:
5254 328 190 100 54
EcoRI G'AATT_C
Cuts at: 3235 3613 3235
Size: 378 5548
EcoRV GAT'ATC
Cuts at: 2866 2866
Size: 5926
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 2127 2276 2314 2700 2768 5073 1678
Size: 449 149 38 386 68 2305 2531
Fragments arranged by size:
2531 2305 449 386 149 68 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2287 2319 2589 2618 2621
Size: 32 135 32 715 32 270 29 3
Cuts at: 2621 2650 4567 4599 4641 4673 1373
Size: 29 1917 32 42 32 2626
Fragments arranged by size:
2626 1917 715 270 135 42 32 32
32 32 32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KasI G'GCGC_C
Cuts at: 2272 2310 2696 2272
Size: 38 386 5502
Fragments arranged by size:
5502 386 38
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 2236 5802 2236
Size: 3566 2360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2022 5040 5224 1305
Size: 717 3018 184 2007
Fragments arranged by size:
3018 2007 717 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2726 10
Size: 54 1425 343 894 3210
Fragments arranged by size:
3210 1425 894 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2379 3884 4243 4402 518
Size: 648 1213 1505 359 159 2042
Fragments arranged by size:
2042 1505 1213 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2057 2338 2761 3342 4019 5167 5412 1606
Size: 451 281 423 581 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 581 451 423 281 245
NarI GG'CG_CC
Cuts at: 2273 2311 2697 2273
Size: 38 386 5502
Fragments arranged by size:
5502 386 38
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5374
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 3284 4829 1132
Size: 97 2055 1545 2229
Fragments arranged by size:
2229 2055 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2418 1348
Size: 1070 4856
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2092 1108
Size: 984 4942
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PmlI CAC'GTG
Cuts at: 2173 2836 2173
Size: 663 5263
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2309 2341 3995 4027 5508 5540 2309
Size: 32 1654 32 1481 32 2695
Fragments arranged by size:
2695 1654 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2422 2804 3094 2422
Size: 382 290 5254
Fragments arranged by size:
5254 382 290
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PspOMI G'GGCC_C
Cuts at: 2749 2749
Size: 5926
PspXI vC'TCGA_Gb
Cuts at: 2203 2203
Size: 5926
PstI C_TGCA'G
Cuts at: 830 3278 830
Size: 2448 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
PvuII CAG'CTG
Cuts at: 2338 2338
Size: 5926
SacII CC_GC'GG
Cuts at: 2058 2058
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SanDI GG'GwC_CC
Cuts at: 2422 2422
Size: 5926
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 3029 4174 5886 3029
Size: 1145 1712 3069
Fragments arranged by size:
3069 1712 1145
SfcI C'TryA_G
Cuts at: 826 1045 2227 3274 4409 5090 5281 826
Size: 219 1182 1047 1135 681 191 1471
Fragments arranged by size:
1471 1182 1135 1047 681 219 191
SfoI GGC'GCC
Cuts at: 2274 2312 2698 2274
Size: 38 386 5502
Fragments arranged by size:
5502 386 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 2755 3253 1872
Size: 883 498 4545
Fragments arranged by size:
4545 883 498
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2203 2499 2849 4030 4931 5193
Size: 197 926 260 296 350 1181 901 262
Cuts at: 5193 820
Size: 1553
Fragments arranged by size:
1553 1181 926 901 350 296 262 260
197
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 3284 1132
Size: 2152 3774
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5926
StyI C'CwwG_G
Cuts at: 513 1065 3063 3365 513
Size: 552 1998 302 3074
Fragments arranged by size:
3074 1998 552 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2126 2198 3953 3970 4123 4308 4753
Size: 1255 546 72 1755 17 153 185 445
Cuts at: 4753 325
Size: 1498
Fragments arranged by size:
1755 1498 1255 546 445 185 153 72
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3027 3165 4172
Size: 274 80 33 51 1443 1050 138 1007
Cuts at: 4172 5884 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1050 1007 274 138 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2775 2807 5886 5918 2775
Size: 32 3079 32 2783
Fragments arranged by size:
3079 2783 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2185 1316
Size: 869 5057
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2887 2974 2887
Size: 87 5839
XhoI C'TCGA_G
Cuts at: 1943 2203 1943
Size: 260 5666
XmaI C'CCGG_G
Cuts at: 1870 2753 3251 1870
Size: 883 498 4545
Fragments arranged by size:
4545 883 498
XmnI GAAnn'nnTTC
Cuts at: 2027 2447 4055 2027
Size: 420 1608 3898
Fragments arranged by size:
3898 1608 420
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PsrI RsrII SacI SapI SexAI SfiI SrfI
SwaI