Restriction map for
pFN21AB4879
(Circular) (Six-base) MAPSORT of: pFN21AB4879.seq Check: 3238 from: 1 to: 7135
pFN21AB4879 7135 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3697 3697
Size: 7135
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2956 4107 4945 278
Size: 53 83 186 936 1420 1151 838 2468
Fragments arranged by size:
2468 1420 1151 936 838 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4478 1937
Size: 2541 4594
Acc65I G'GTAC_C
Cuts at: 4456 4456
Size: 7135
AclI AA'CG_TT
Cuts at: 1563 5262 5635 1563
Size: 3699 373 3063
Fragments arranged by size:
3699 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2154 2909 3338 3807 5198 6582 6850 1632
Size: 522 755 429 469 1391 1384 268 1917
Fragments arranged by size:
1917 1391 1384 755 522 469 429 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6938
AflIII A'CryG_T
Cuts at: 1203 3456 4283 4383 6034 7011 1203
Size: 2253 827 100 1651 977 1327
Fragments arranged by size:
2253 1651 1327 977 827 100
AgeI A'CCGG_T
Cuts at: 5966 5966
Size: 7135
AhdI GACnn_n'nnGTC
Cuts at: 5864 5864
Size: 7135
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3582 1184
Size: 2398 4737
AlwNI CAG_nnn'CTG
Cuts at: 2132 2161 2408 3647 4878 6450 2132
Size: 29 247 1239 1231 1572 2817
Fragments arranged by size:
2817 1572 1239 1231 247 29
ApaLI G'TGCA_C
Cuts at: 5192 6348 5192
Size: 1156 5979
ApoI r'AATT_y
Cuts at: 1493 2041 2354 2845 3500 3521 4444 4670 4822
Size: 548 313 491 655 21 923 226 152
Cuts at: 4822 6931 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 923 655 548 491 313 226
152 21
AseI AT'TA_AT
Cuts at: 160 1032 5689 160
Size: 872 4657 1606
Fragments arranged by size:
4657 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7135
AvaI C'yCGr_G
Cuts at: 1870 1943 4460 1870
Size: 73 2517 4545
Fragments arranged by size:
4545 2517 73
AvrII C'CTAG_G
Cuts at: 3241 3241
Size: 7135
BamHI G'GATC_C
Cuts at: 3515 4465 4832 3515
Size: 950 367 5818
Fragments arranged by size:
5818 950 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4456 5911 618
Size: 325 217 627 2669 1455 1842
Fragments arranged by size:
2669 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3834 4454 1391
Size: 2443 620 4072
Fragments arranged by size:
4072 2443 620
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2202 2754 2904 2915 3807 4046 928
Size: 971 303 552 150 11 892 239 4017
Fragments arranged by size:
4017 971 892 552 303 239 150 11
BbvCI CC'TCA_GC
Cuts at: 2103 2103
Size: 7135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5326 5360 1903
Size: 34 3389 34 3678
Fragments arranged by size:
3678 3389 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5027 6237 5027
Size: 1210 5925
BclI T'GATC_A
Cuts at: 1548 4305 4333 1548
Size: 2757 28 4350
Fragments arranged by size:
4350 2757 28
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5746 136
Size: 107 122 71 631 605 4074 1525
Fragments arranged by size:
4074 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7130 7130
Size: 7135
BlpI GC'TnA_GC
Cuts at: 3822 4552 3822
Size: 730 6405
Bme1580I G_kGCm'C
Cuts at: 1790 5196 6352 1790
Size: 3406 1156 2573
Fragments arranged by size:
3406 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7135
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3023 3402 4866
Size: 416 53 83 399 24 1606 379 1464
Cuts at: 4866 5824 442
Size: 958 1753
Fragments arranged by size:
1753 1606 1464 958 416 399 379 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3123 3384 5795 1239
Size: 1884 261 2411 2579
Fragments arranged by size:
2579 2411 1884 261
Bpu10I CC'TnA_GC
Cuts at: 2103 2478 2694 2977 3144 3813 2103
Size: 375 216 283 167 669 5425
Fragments arranged by size:
5425 669 375 283 216 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3185 3528 5260 6125 6423 3185
Size: 343 1732 865 298 3897
Fragments arranged by size:
3897 1732 865 343 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4118 4214 4409 5798 882
Size: 617 2619 96 195 1389 2219
Fragments arranged by size:
2619 2219 1389 617 195 96
BsaAI yAC'GTr
Cuts at: 493 1204 4286 4926 493
Size: 711 3082 640 2702
Fragments arranged by size:
3082 2702 711 640
BsaBI GATnn'nnATC
Cuts at: 3472 7129 3472
Size: 3657 3478
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2953 3620 3723 4104
Size: 53 83 186 936 1420 667 103 381
Cuts at: 4104 4942 5324 5959 275
Size: 838 382 635 1451
Fragments arranged by size:
1451 1420 936 838 667 635 382 381
186 103 83 53
BsaWI w'CCGG_w
Cuts at: 2413 5568 5966 6240 6387 6798 2413
Size: 3155 398 274 147 411 2750
Fragments arranged by size:
3155 2750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6910
Fragments arranged by size:
6910 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2245 3884 1186
Size: 1059 1639 4437
Fragments arranged by size:
4437 1639 1059
BseYI C'CCAG_C
Cuts at: 2881 6338 6834 2881
Size: 3457 496 3182
Fragments arranged by size:
3457 3182 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2481 3669 1163
Size: 573 745 1188 4629
Fragments arranged by size:
4629 1188 745 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4585 5346 6374 1540
Size: 275 186 2584 761 1028 2301
Fragments arranged by size:
2584 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2543 3571 5196 5281 6352 2543
Size: 1028 1625 85 1071 3326
Fragments arranged by size:
3326 1625 1071 1028 85
BsmI GAATG_Cn'
Cuts at: 3175 4646 4739 3175
Size: 1471 93 5571
Fragments arranged by size:
5571 1471 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2543 3571 3834 4454 5196 5281 6352
Size: 399 753 1028 263 620 742 85 1071
Cuts at: 6352 1391
Size: 2174
Fragments arranged by size:
2174 1071 1028 753 742 620 399 263
85
BspEI T'CCGG_A
Cuts at: 6798 6798
Size: 7135
BspHI T'CATG_A
Cuts at: 5024 6754 5024
Size: 1730 5405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2815 3117 3697 4490 844
Size: 1971 302 580 793 3489
Fragments arranged by size:
3489 1971 793 580 302
BsrBI CCG'CTC
Cuts at: 1394 1823 2218 5022 1394
Size: 429 395 2804 3507
Fragments arranged by size:
3507 2804 429 395
BsrDI GCAATG_nn'
Cuts at: 65 1248 5630 5804 65
Size: 1183 4382 174 1396
Fragments arranged by size:
4382 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5779 5966 1700
Size: 110 3969 187 2869
Fragments arranged by size:
3969 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4345 96
Size: 4249 2886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7135
BssSI C'ACGA_G
Cuts at: 5195 6207 5195
Size: 1012 6123
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7135
BstBI TT'CG_AA
Cuts at: 5939 5939
Size: 7135
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7135
BstYI r'GATC_y
Cuts at: 1972 2440 2497 3515 4465 4832 5217 5234 6686
Size: 468 57 1018 950 367 385 17 1452
Cuts at: 6686 6772 6784 7130 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1018 950 468 385 367 346
86 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3037 3938 513
Size: 552 1972 901 3710
Fragments arranged by size:
3710 1972 901 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6396
Fragments arranged by size:
6396 683 56
BtsI GCAGTG_nn'
Cuts at: 2464 2635 4651 5445 5465 2464
Size: 171 2016 794 20 4134
Fragments arranged by size:
4134 2016 794 171 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7135
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7100
DraI TTT'AAA
Cuts at: 2388 3064 4439 2388
Size: 676 1375 5084
Fragments arranged by size:
5084 1375 676
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4187 6142 809
Size: 507 2871 1955 1802
Fragments arranged by size:
2871 1955 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3535 4582 5471
Size: 54 1425 50 275 18 1705 1047 889
Cuts at: 5471 7100 8
Size: 1629 43
Fragments arranged by size:
1705 1629 1425 1047 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4582 1537
Size: 275 2770 4090
Fragments arranged by size:
4090 2770 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2012 2058 2370 2529 3090 5065 1353
Size: 659 46 312 159 561 1975 3423
Fragments arranged by size:
3423 1975 659 561 312 159 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2794 5722 6096 6242 387
Size: 1064 138 1205 2928 374 146 1280
Fragments arranged by size:
2928 1280 1205 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2909 3123 3338 3384 3807 5198
Size: 393 522 755 214 215 46 423 1391
Cuts at: 5198 5795 6582 6850 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1391 787 755 597 522 423 393
268 215 214 46
EcoO109I rG'GnC_Cy
Cuts at: 2392 4025 4339 2392
Size: 1633 314 5188
Fragments arranged by size:
5188 1633 314
EcoRI G'AATT_C
Cuts at: 4444 4822 4444
Size: 378 6757
EcoRV GAT'ATC
Cuts at: 3235 3235
Size: 7135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2726 2726
Size: 7135
FspI TGC'GCA
Cuts at: 5641 5641
Size: 7135
HaeII r_GCGC'y
Cuts at: 1678 6282 1678
Size: 4604 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2207 2239 3127 3159 3219
Size: 32 135 32 635 32 888 32 60
Cuts at: 3219 3251 3532 3564 5776 5808 5850 5882 1373
Size: 32 281 32 2212 32 42 32 2626
Fragments arranged by size:
2626 2212 888 635 281 135 60 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2892 4479 4725 669
Size: 988 281 954 1587 246 3079
Fragments arranged by size:
3079 1587 988 954 281 246
HindIII A'AGCT_T
Cuts at: 748 2166 2598 748
Size: 1418 432 5285
Fragments arranged by size:
5285 1418 432
HpaI GTT'AAC
Cuts at: 2892 4725 2892
Size: 1833 5302
KpnI G_GTAC'C
Cuts at: 4460 4460
Size: 7135
MfeI C'AATT_G
Cuts at: 2397 4734 2397
Size: 2337 4798
MluI A'CGCG_T
Cuts at: 7011 7011
Size: 7135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2634 6249 6433 1305
Size: 1329 3615 184 2007
Fragments arranged by size:
3615 2007 1329 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5313
Fragments arranged by size:
5313 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4388 5093 5452 5611 518
Size: 648 3222 705 359 159 2042
Fragments arranged by size:
3222 2042 705 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2132 3647 4551 5228 6376 6621 1606
Size: 526 1515 904 677 1148 245 2120
Fragments arranged by size:
2120 1515 1148 904 677 526 245
NcoI C'CATG_G
Cuts at: 513 1065 3037 513
Size: 552 1972 4611
Fragments arranged by size:
4611 1972 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7135
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7135
NotI GC'GGCC_GC
Cuts at: 4582 4582
Size: 7135
NspI r_CATG'y
Cuts at: 1132 1229 2635 2693 4387 4493 6038 1132
Size: 97 1406 58 1694 106 1545 2229
Fragments arranged by size:
2229 1694 1545 1406 106 97 58
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7135
PciI A'CATG_T
Cuts at: 4383 6034 4383
Size: 1651 5484
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3043 1108
Size: 1935 5200
PfoI T'CCnGG_A
Cuts at: 3623 3623
Size: 7135
PmeI GTTT'AAAC
Cuts at: 4439 4439
Size: 7135
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2403 2435 5204 5236 6717 6749 2403
Size: 32 2769 32 1481 32 2789
Fragments arranged by size:
2789 2769 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4025 4339 4025
Size: 314 6821
PshAI GACnn'nnGTC
Cuts at: 1613 3666 3861 1613
Size: 2053 195 4887
Fragments arranged by size:
4887 2053 195
PsiI TTA'TAA
Cuts at: 3330 4705 3330
Size: 1375 5760
PstI C_TGCA'G
Cuts at: 830 4487 830
Size: 3657 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7135
PvuII CAG'CTG
Cuts at: 2132 3647 2132
Size: 1515 5620
SalI G'TCGA_C
Cuts at: 1936 4477 1936
Size: 2541 4594
SbfI CC_TGCA'GG
Cuts at: 4487 4487
Size: 7135
ScaI AGT'ACT
Cuts at: 5383 7095 5383
Size: 1712 5423
SfcI C'TryA_G
Cuts at: 826 1045 3477 4483 5618 6299 6490 826
Size: 219 2432 1006 1135 681 191 1471
Fragments arranged by size:
2432 1471 1135 1006 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7135
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7135
SmaI CCC'GGG
Cuts at: 1872 4462 1872
Size: 2590 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3164 3543 5239 6140 6402 820
Size: 197 926 1221 379 1696 901 262 1553
Fragments arranged by size:
1696 1553 1221 926 901 379 262 197
SnaBI TAC'GTA
Cuts at: 493 4926 493
Size: 4433 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7135
SphI G_CATG'C
Cuts at: 1132 4493 1132
Size: 3361 3774
SspI AAT'ATT
Cuts at: 5 52 5059 5
Size: 47 5007 2081
Fragments arranged by size:
5007 2081 47
StuI AGG'CCT
Cuts at: 1517 3583 1517
Size: 2066 5069
StyI C'CwwG_G
Cuts at: 513 1065 2840 3037 3084 3241 3302 4395 4574
Size: 552 1775 197 47 157 61 1093 179
Cuts at: 4574 513
Size: 3074
Fragments arranged by size:
3074 1775 1093 552 197 179 157 61
47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3678 4392 4433 5162 5179 5332 5517
Size: 1255 2098 714 41 729 17 153 185
Cuts at: 5517 5962 325
Size: 445 1498
Fragments arranged by size:
2098 1498 1255 729 714 445 185 153
41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4345 5381 7093
Size: 274 80 33 51 1443 2368 1036 1712
Cuts at: 7093 96
Size: 138
Fragments arranged by size:
2368 1712 1443 1036 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3427 3459 7095 7127 3427
Size: 32 3636 32 3435
Fragments arranged by size:
3636 3435 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7135
XbaI T'CTAG_A
Cuts at: 4471 4471
Size: 7135
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7135
XmaI C'CCGG_G
Cuts at: 1870 4460 1870
Size: 2590 4545
XmnI GAAnn'nnTTC
Cuts at: 5264 5264
Size: 7135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2954 4105 4943 276
Size: 53 83 186 936 1420 1151 838 2468
Fragments arranged by size:
2468 1420 1151 936 838 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI