Restriction map for
pFN21AB4878
(Circular) (Six-base) MAPSORT of: pFN21AB4878.seq Check: 9462 from: 1 to: 6238
pFN21AB4878 6238 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4048 278
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3581 1937
Size: 1644 4594
Acc65I G'GTAC_C
Cuts at: 3559 3559
Size: 6238
AclI AA'CG_TT
Cuts at: 1563 4365 4738 1563
Size: 2802 373 3063
Fragments arranged by size:
3063 2802 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4301 5685 5953 1632
Size: 2669 1384 268 1917
Fragments arranged by size:
2669 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6041
AflIII A'CryG_T
Cuts at: 1203 5137 6114 1203
Size: 3934 977 1327
Fragments arranged by size:
3934 1327 977
AgeI A'CCGG_T
Cuts at: 5069 5069
Size: 6238
AhdI GACnn_n'nnGTC
Cuts at: 4967 4967
Size: 6238
AleI CACnn'nnGTG
Cuts at: 1166 2567 2957 1166
Size: 1401 390 4447
Fragments arranged by size:
4447 1401 390
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6238
AlwNI CAG_nnn'CTG
Cuts at: 3247 3981 5553 3247
Size: 734 1572 3932
Fragments arranged by size:
3932 1572 734
ApaLI G'TGCA_C
Cuts at: 4295 5451 4295
Size: 1156 5082
ApoI r'AATT_y
Cuts at: 1493 2557 2621 3547 3773 3925 6034 1493
Size: 1064 64 926 226 152 2109 1697
Fragments arranged by size:
2109 1697 1064 926 226 152 64
AseI AT'TA_AT
Cuts at: 160 1032 4792 160
Size: 872 3760 1606
Fragments arranged by size:
3760 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
AvaI C'yCGr_G
Cuts at: 1870 1943 3563 1870
Size: 73 1620 4545
Fragments arranged by size:
4545 1620 73
BamHI G'GATC_C
Cuts at: 3568 3935 3568
Size: 367 5871
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3303 3344 3559 5014 618
Size: 325 217 627 1516 41 215 1455 1842
Fragments arranged by size:
1842 1516 1455 627 325 217 215 41
BanII G_rGCy'C
Cuts at: 1391 2769 2937 3557 1391
Size: 1378 168 620 4072
Fragments arranged by size:
4072 1378 620 168
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2680 2810 3370 928
Size: 971 781 130 560 3796
Fragments arranged by size:
3796 971 781 560 130
BbvCI CC'TCA_GC
Cuts at: 2552 2552
Size: 6238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4429 4463 1903
Size: 34 2492 34 3678
Fragments arranged by size:
3678 2492 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4130 5340 4130
Size: 1210 5028
BclI T'GATC_A
Cuts at: 1548 3315 1548
Size: 1767 4471
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4849 136
Size: 107 122 71 631 605 3177 1525
Fragments arranged by size:
3177 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6233 6233
Size: 6238
BlpI GC'TnA_GC
Cuts at: 2080 3655 2080
Size: 1575 4663
Bme1580I G_kGCm'C
Cuts at: 1790 3306 4299 5455 1790
Size: 1516 993 1156 2573
Fragments arranged by size:
2573 1516 1156 993
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3309 3969 4927
Size: 416 53 83 399 24 1892 660 958
Cuts at: 4927 442
Size: 1753
Fragments arranged by size:
1892 1753 958 660 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2519 4898 1239
Size: 1280 2379 2579
Fragments arranged by size:
2579 2379 1280
Bpu10I CC'TnA_GC
Cuts at: 2552 2552
Size: 6238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3286 4363 5228 5526 3286
Size: 1077 865 298 3998
Fragments arranged by size:
3998 1077 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2657 4901 882
Size: 617 1158 2244 2219
Fragments arranged by size:
2244 2219 1158 617
BsaAI yAC'GTr
Cuts at: 493 1204 4029 493
Size: 711 2825 2702
Fragments arranged by size:
2825 2702 711
BsaBI GATnn'nnATC
Cuts at: 6232 6232
Size: 6238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4045 4427 5062 275
Size: 53 83 186 936 2512 382 635 1451
Fragments arranged by size:
2512 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2691 4671 5069 5343 5490 5901 2691
Size: 1980 398 274 147 411 3028
Fragments arranged by size:
3028 1980 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6013
Fragments arranged by size:
6013 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 1186
Size: 1000 5238
BseYI C'CCAG_C
Cuts at: 2140 2249 2574 3331 5441 5937 2140
Size: 109 325 757 2110 496 2441
Fragments arranged by size:
2441 2110 757 496 325 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2540 1163
Size: 573 804 4861
Fragments arranged by size:
4861 804 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3688 4449 5477 1540
Size: 275 186 1687 761 1028 2301
Fragments arranged by size:
2301 1687 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2727 2769 2781 4299 4384 5455 2727
Size: 42 12 1518 85 1071 3510
Fragments arranged by size:
3510 1518 1071 85 42 12
BsmI GAATG_Cn'
Cuts at: 3749 3842 3749
Size: 93 6145
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2727 2769 2781 2937 3306 3557 4299
Size: 399 937 42 12 156 369 251 742
Cuts at: 4299 4384 5455 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 937 742 399 369 251 156
85 42 12
BspEI T'CCGG_A
Cuts at: 2691 5901 2691
Size: 3210 3028
BspHI T'CATG_A
Cuts at: 2193 4127 5857 2193
Size: 1934 1730 2574
Fragments arranged by size:
2574 1934 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3593 844
Size: 2749 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 4125 1394
Size: 429 206 2096 3507
Fragments arranged by size:
3507 2096 429 206
BsrDI GCAATG_nn'
Cuts at: 65 1248 2070 4733 4907 65
Size: 1183 822 2663 174 1396
Fragments arranged by size:
2663 1396 1183 822 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2755 4882 5069 1700
Size: 110 945 2127 187 2869
Fragments arranged by size:
2869 2127 945 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6238
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6238
BssSI C'ACGA_G
Cuts at: 4298 5310 4298
Size: 1012 5226
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6238
BstBI TT'CG_AA
Cuts at: 5042 5042
Size: 6238
BstEII G'GTnAC_C
Cuts at: 1184 2569 1184
Size: 1385 4853
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2351 2896 2923 2959 3292 2011
Size: 340 545 27 36 333 4957
Fragments arranged by size:
4957 545 340 333 36 27
BstYI r'GATC_y
Cuts at: 1972 2885 3568 3935 4320 4337 5789 5875 5887
Size: 913 683 367 385 17 1452 86 12
Cuts at: 5887 6233 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 913 683 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3410 3410
Size: 6238
BtgI C'CryG_G
Cuts at: 513 1065 2928 2952 3154 3309 513
Size: 552 1863 24 202 155 3442
Fragments arranged by size:
3442 1863 552 202 155 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2890 487
Size: 683 56 1664 3835
Fragments arranged by size:
3835 1664 683 56
BtsI GCAGTG_nn'
Cuts at: 2401 3754 4548 4568 2401
Size: 1353 794 20 4071
Fragments arranged by size:
4071 1353 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6203
DraI TTT'AAA
Cuts at: 3542 3542
Size: 6238
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5245 809
Size: 507 3929 1802
Fragments arranged by size:
3929 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2030 2178 2925
Size: 54 1425 50 275 18 200 148 747
Cuts at: 2925 2995 3151 3294 3429 3685 4574 6203 8
Size: 70 156 143 135 256 889 1629 43
Fragments arranged by size:
1629 1425 889 747 275 256 200 156
148 143 135 70 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3685 1537
Size: 275 1873 4090
Fragments arranged by size:
4090 1873 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2784 3494 4168 1353
Size: 1431 710 674 3423
Fragments arranged by size:
3423 1431 710 674
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4825 5199 5345 387
Size: 1064 138 3236 374 146 1280
Fragments arranged by size:
3236 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2767 2767
Size: 6238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2519 4301 4898 5685 5953 1239
Size: 393 887 1782 597 787 268 1524
Fragments arranged by size:
1782 1524 887 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2232 3414 2232
Size: 1182 5056
EcoRI G'AATT_C
Cuts at: 3547 3925 3547
Size: 378 5860
FspI TGC'GCA
Cuts at: 4744 4744
Size: 6238
HaeII r_GCGC'y
Cuts at: 1678 5385 1678
Size: 3707 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 2670 2702 2870
Size: 32 135 32 434 32 632 32 168
Cuts at: 2870 2902 4879 4911 4953 4985 1373
Size: 32 1977 32 42 32 2626
Fragments arranged by size:
2626 1977 632 434 168 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3582 3828 669
Size: 988 281 1644 246 3079
Fragments arranged by size:
3079 1644 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6238
HpaI GTT'AAC
Cuts at: 3828 3828
Size: 6238
KpnI G_GTAC'C
Cuts at: 3563 3563
Size: 6238
MfeI C'AATT_G
Cuts at: 3837 3837
Size: 6238
MluI A'CGCG_T
Cuts at: 6114 6114
Size: 6238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3441 5352 5536 1305
Size: 2136 1911 184 2007
Fragments arranged by size:
2136 2007 1911 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2180 2927 2997 3296 3431
Size: 54 1425 343 348 747 70 299 135
Cuts at: 3431 10
Size: 2817
Fragments arranged by size:
2817 1425 747 348 343 299 135 70
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2343 2567 2921 2957 3290 4196
Size: 648 843 334 224 354 36 333 906
Cuts at: 4196 4555 4714 518
Size: 359 159 2042
Fragments arranged by size:
2042 906 843 648 359 354 334 333
224 159 36
MspA1I CmG'CkG
Cuts at: 1606 3427 3654 4331 5479 5724 1606
Size: 1821 227 677 1148 245 2120
Fragments arranged by size:
2120 1821 1148 677 245 227
NcoI C'CATG_G
Cuts at: 513 1065 2928 3154 3309 513
Size: 552 1863 226 155 3442
Fragments arranged by size:
3442 1863 552 226 155
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6238
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6238
NotI GC'GGCC_GC
Cuts at: 3685 3685
Size: 6238
NspI r_CATG'y
Cuts at: 1132 1229 2257 2701 3596 5141 1132
Size: 97 1028 444 895 1545 2229
Fragments arranged by size:
2229 1545 1028 895 444 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6238
PciI A'CATG_T
Cuts at: 5137 5137
Size: 6238
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6238
PfoI T'CCnGG_A
Cuts at: 3197 3197
Size: 6238
PmeI GTTT'AAAC
Cuts at: 3542 3542
Size: 6238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 5820 5852 4307
Size: 32 1481 32 4693
Fragments arranged by size:
4693 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2232 3414 2232
Size: 1182 5056
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6238
PsiI TTA'TAA
Cuts at: 3808 3808
Size: 6238
PstI C_TGCA'G
Cuts at: 830 3590 830
Size: 2760 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6238
PvuII CAG'CTG
Cuts at: 3427 3427
Size: 6238
SacI G_AGCT'C
Cuts at: 2769 2769
Size: 6238
SalI G'TCGA_C
Cuts at: 1936 3580 1936
Size: 1644 4594
SbfI CC_TGCA'GG
Cuts at: 3590 3590
Size: 6238
ScaI AGT'ACT
Cuts at: 4486 6198 4486
Size: 1712 4526
SexAI A'CCwGG_T
Cuts at: 2973 3240 2973
Size: 267 5971
SfcI C'TryA_G
Cuts at: 826 1045 2392 3279 3586 4721 5402 5593 826
Size: 219 1347 887 307 1135 681 191 1471
Fragments arranged by size:
1471 1347 1135 887 681 307 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6238
SmaI CCC'GGG
Cuts at: 1872 3565 1872
Size: 1693 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3265 4342 5243 5505 820
Size: 197 926 1322 1077 901 262 1553
Fragments arranged by size:
1553 1322 1077 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4029 493
Size: 3536 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6238
SphI G_CATG'C
Cuts at: 1132 2257 2701 3596 1132
Size: 1125 444 895 3774
Fragments arranged by size:
3774 1125 895 444
SspI AAT'ATT
Cuts at: 5 52 2305 4162 5
Size: 47 2253 1857 2081
Fragments arranged by size:
2253 2081 1857 47
StuI AGG'CCT
Cuts at: 1517 2398 1517
Size: 881 5357
StyI C'CwwG_G
Cuts at: 513 1065 2166 2423 2928 3039 3154 3309 3677
Size: 552 1101 257 505 111 115 155 368
Cuts at: 3677 513
Size: 3074
Fragments arranged by size:
3074 1101 552 505 368 257 155 115
111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2263 2469 4265 4282 4435 4620 5065
Size: 1255 683 206 1796 17 153 185 445
Cuts at: 5065 325
Size: 1498
Fragments arranged by size:
1796 1498 1255 683 445 206 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3447 4484 6196
Size: 274 80 33 51 1443 1470 1037 1712
Cuts at: 6196 96
Size: 138
Fragments arranged by size:
1712 1470 1443 1037 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6198 6230 6198
Size: 32 6206
Tth111I GACn'n_nGTC
Cuts at: 1316 2684 1316
Size: 1368 4870
XbaI T'CTAG_A
Cuts at: 3574 3574
Size: 6238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2173 2173
Size: 6238
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6238
XmaI C'CCGG_G
Cuts at: 1870 3563 1870
Size: 1693 4545
XmnI GAAnn'nnTTC
Cuts at: 4367 4367
Size: 6238
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4046 276
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI