Restriction map for
pFN21AB4875
(Circular) (Six-base) MAPSORT of: pFN21AB4875.seq Check: 2136 from: 1 to: 7831
pFN21AB4875 7831 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5641 278
Size: 53 83 186 936 4105 2468
Fragments arranged by size:
4105 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5174 1937
Size: 3237 4594
Acc65I G'GTAC_C
Cuts at: 2953 5152 2953
Size: 2199 5632
AclI AA'CG_TT
Cuts at: 1563 5958 6331 1563
Size: 4395 373 3063
Fragments arranged by size:
4395 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2430 5894 7278 7546 1632
Size: 798 3464 1384 268 1917
Fragments arranged by size:
3464 1917 1384 798 268
AfeI AGC'GCT
Cuts at: 3537 4142 3537
Size: 605 7226
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7634
AflIII A'CryG_T
Cuts at: 1203 3225 3372 6730 7707 1203
Size: 2022 147 3358 977 1327
Fragments arranged by size:
3358 2022 1327 977 147
AgeI A'CCGG_T
Cuts at: 6662 6662
Size: 7831
AhdI GACnn_n'nnGTC
Cuts at: 6560 6560
Size: 7831
AleI CACnn'nnGTG
Cuts at: 1166 3748 4133 1166
Size: 2582 385 4864
Fragments arranged by size:
4864 2582 385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3839 1184
Size: 2655 5176
AlwNI CAG_nnn'CTG
Cuts at: 2318 3610 3943 4210 4246 4373 4426 4466 4916
Size: 1292 333 267 36 127 53 40 450
Cuts at: 4916 5574 7146 2318
Size: 658 1572 3003
Fragments arranged by size:
3003 1572 1292 658 450 333 267 127
53 40 36
ApaI G_GGCC'C
Cuts at: 2150 2723 2862 3148 3716 4366 2150
Size: 573 139 286 568 650 5615
Fragments arranged by size:
5615 650 573 568 286 139
ApaLI G'TGCA_C
Cuts at: 5888 7044 5888
Size: 1156 6675
ApoI r'AATT_y
Cuts at: 1493 5140 5366 5518 7627 1493
Size: 3647 226 152 2109 1697
Fragments arranged by size:
3647 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 4025 4181 4025
Size: 156 7675
AseI AT'TA_AT
Cuts at: 160 1032 6385 160
Size: 872 5353 1606
Fragments arranged by size:
5353 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7831
AvaI C'yCGr_G
Cuts at: 1870 1943 2510 3708 3770 4175 4226 4845 5156
Size: 73 567 1198 62 405 51 619 311
Cuts at: 5156 1870
Size: 4545
Fragments arranged by size:
4545 1198 619 567 405 311 73 62
51
BamHI G'GATC_C
Cuts at: 5161 5528 5161
Size: 367 7464
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2488 2771 2828 2902 2953
Size: 325 217 627 701 283 57 74 51
Cuts at: 2953 3693 3719 3766 3823 3868 4255 4687 5152
Size: 740 26 47 57 45 387 432 465
Cuts at: 5152 6607 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 740 701 627 465 432 387
325 283 217 74 57 57 51 47
45 26
BanII G_rGCy'C
Cuts at: 1391 2036 2150 2392 2511 2723 2862 2918 2939
Size: 645 114 242 119 212 139 56 21
Cuts at: 2939 3041 3148 3716 3743 3796 4366 5111 5150
Size: 102 107 568 27 53 570 745 39
Cuts at: 5150 1391
Size: 4072
Fragments arranged by size:
4072 745 645 570 568 242 212 139
119 114 107 102 56 53 39 27
21
BbeI G_GCGC'C
Cuts at: 2906 3697 3723 3770 3872 4259 4691 2906
Size: 791 26 47 102 387 432 6046
Fragments arranged by size:
6046 791 432 387 102 47 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6860
BbvCI CC'TCA_GC
Cuts at: 4697 4697
Size: 7831
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3961 3995 4217 4251 6022
Size: 34 50 34 1940 34 222 34 1771
Cuts at: 6022 6056 1903
Size: 34 3678
Fragments arranged by size:
3678 1940 1771 222 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5723 6933 5723
Size: 1210 6621
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7831
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2101 2857 3030
Size: 107 122 71 631 605 429 756 173
Cuts at: 3030 3288 3791 3813 3837 4098 4160 4354 5126
Size: 258 503 22 24 261 62 194 772
Cuts at: 5126 6442 136
Size: 1316 1525
Fragments arranged by size:
1525 1316 772 756 631 605 503 429
261 258 194 173 122 107 71 62
24 22
BglII A'GATC_T
Cuts at: 7826 7826
Size: 7831
BlpI GC'TnA_GC
Cuts at: 2912 2938 2990 4905 5248 2912
Size: 26 52 1915 343 5495
Fragments arranged by size:
5495 1915 343 52 26
Bme1580I G_kGCm'C
Cuts at: 1790 2150 2215 2221 2491 2562 2723 2774 2862
Size: 360 65 6 270 71 161 51 88
Cuts at: 2862 3148 3394 3716 3828 4366 5892 7048 1790
Size: 286 246 322 112 538 1526 1156 2573
Fragments arranged by size:
2573 1526 1156 538 360 322 286 270
246 161 112 88 71 65 51 6
BmgBI CAC'GTC
Cuts at: 1318 2365 3913 1318
Size: 1047 1548 5236
Fragments arranged by size:
5236 1548 1047
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4544 4605 4653
Size: 416 53 83 399 24 3127 61 48
Cuts at: 4653 5562 6520 442
Size: 909 958 1753
Fragments arranged by size:
3127 1753 958 909 416 399 83 61
53 48 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7831
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2446 4828 4954 2446
Size: 2382 126 5323
Fragments arranged by size:
5323 2382 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2615 2651 2912 4443 4799 4853 5030 6491
Size: 1376 36 261 1531 356 54 177 1461
Cuts at: 6491 1239
Size: 2579
Fragments arranged by size:
2579 1531 1461 1376 356 261 177 54
36
Bpu10I CC'TnA_GC
Cuts at: 3795 4697 4958 3795
Size: 902 261 6668
Fragments arranged by size:
6668 902 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2357 5956 6821 7119 2357
Size: 3599 865 298 3069
Fragments arranged by size:
3599 3069 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2403 4049 4869 4942 6494 882
Size: 617 904 1646 820 73 1552 2219
Fragments arranged by size:
2219 1646 1552 904 820 617 73
BsaAI yAC'GTr
Cuts at: 493 1204 2899 3226 3373 4135 4718 5622 493
Size: 711 1695 327 147 762 583 904 2702
Fragments arranged by size:
2702 1695 904 762 711 583 327 147
BsaBI GATnn'nnATC
Cuts at: 7825 7825
Size: 7831
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2903 3694 3720 3767
Size: 53 83 186 936 1370 791 26 47
Cuts at: 3767 3869 4113 4256 4688 5638 6020 6655 275
Size: 102 244 143 432 950 382 635 1451
Fragments arranged by size:
1451 1370 950 936 791 635 432 382
244 186 143 102 83 53 47 26
BsaWI w'CCGG_w
Cuts at: 2814 3273 6264 6662 6936 7083 7494 2814
Size: 459 2991 398 274 147 411 3151
Fragments arranged by size:
3151 2991 459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3038 3068 4772 4802 4826
Size: 30 165 30 1635 30 1704 30 24
Cuts at: 4826 4856 5070 5100 1178
Size: 30 214 30 3909
Fragments arranged by size:
3909 1704 1635 214 165 30 30 30
30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2612 2762 3059 3062 3260 4496 4568 5027
Size: 1426 150 297 3 198 1236 72 459
Cuts at: 5027 5030 5075 1186
Size: 3 45 3942
Fragments arranged by size:
3942 1426 1236 459 297 198 150 72
45 3 3
BseYI C'CCAG_C
Cuts at: 2290 2716 2918 3103 3147 3835 4214 4239 5104
Size: 426 202 185 44 688 379 25 865
Cuts at: 5104 7034 7530 2290
Size: 1930 496 2591
Fragments arranged by size:
2591 1930 865 688 496 426 379 202
185 44 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2550 2762 3388 4397 4412 4484 5091
Size: 573 814 212 626 1009 15 72 607
Cuts at: 5091 1163
Size: 3903
Fragments arranged by size:
3903 1009 814 626 607 573 212 72
15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2583 3558 3849 3883 3961 4300
Size: 275 186 582 975 291 34 78 339
Cuts at: 4300 5281 6042 7070 1540
Size: 981 761 1028 2301
Fragments arranged by size:
2301 1028 981 975 761 582 339 291
275 186 78 34
BsiHKAI G_wGCw'C
Cuts at: 2511 2637 2898 5892 5977 7048 2511
Size: 126 261 2994 85 1071 3294
Fragments arranged by size:
3294 2994 1071 261 126 85
BsmI GAATG_Cn'
Cuts at: 5342 5435 5342
Size: 93 7738
BsmBI CGTCTCn'nnnn_
Cuts at: 4151 4151
Size: 7831
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2150 2215 2221 2392 2491 2511
Size: 399 246 114 65 6 171 99 20
Cuts at: 2511 2562 2637 2723 2774 2862 2898 2918 2939
Size: 51 75 86 51 88 36 20 21
Cuts at: 2939 3041 3148 3394 3716 3743 3796 3828 4366
Size: 102 107 246 322 27 53 32 538
Cuts at: 4366 5111 5150 5892 5977 7048 1391
Size: 745 39 742 85 1071 2174
Fragments arranged by size:
2174 1071 745 742 538 399 322 246
246 171 114 107 102 99 88 86
85 75 65 53 51 51 39 36
32 27 21 20 20 6
BspEI T'CCGG_A
Cuts at: 2814 3273 7494 2814
Size: 459 4221 3151
Fragments arranged by size:
4221 3151 459
BspHI T'CATG_A
Cuts at: 5720 7450 5720
Size: 1730 6101
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2254 2484 3618 4986 5186 844
Size: 1410 230 1134 1368 200 3489
Fragments arranged by size:
3489 1410 1368 1134 230 200
BsrBI CCG'CTC
Cuts at: 1394 1823 3357 3804 4296 5718 1394
Size: 429 1534 447 492 1422 3507
Fragments arranged by size:
3507 1534 1422 492 447 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6326 6500 65
Size: 1183 5078 174 1396
Fragments arranged by size:
5078 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 3076 3557 4299 4353 5043 6475
Size: 110 208 1058 481 742 54 690 1432
Cuts at: 6475 6662 1700
Size: 187 2869
Fragments arranged by size:
2869 1432 1058 742 690 481 208 187
110 54
BsrGI T'GTAC_A
Cuts at: 96 3054 4326 96
Size: 2958 1272 3601
Fragments arranged by size:
3601 2958 1272
BssHII G'CGCG_C
Cuts at: 1925 3566 3760 4025 4181 1925
Size: 1641 194 265 156 5575
Fragments arranged by size:
5575 1641 265 194 156
BssSI C'ACGA_G
Cuts at: 3209 5891 6903 3209
Size: 2682 1012 4137
Fragments arranged by size:
4137 2682 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2374 3610 4426 794
Size: 1580 1236 816 4199
Fragments arranged by size:
4199 1580 1236 816
BstBI TT'CG_AA
Cuts at: 6635 6635
Size: 7831
BstEII G'GTnAC_C
Cuts at: 1184 3634 4396 1184
Size: 2450 762 4619
Fragments arranged by size:
4619 2450 762
BstXI CCAn_nnnn'nTGG
Cuts at: 3323 5104 3323
Size: 1781 6050
BstYI r'GATC_y
Cuts at: 1972 4369 4471 5161 5528 5913 5930 7382 7468
Size: 2397 102 690 367 385 17 1452 86
Cuts at: 7468 7480 7826 1972
Size: 12 346 1977
Fragments arranged by size:
2397 1977 1452 690 385 367 346 102
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4881 4881
Size: 7831
BtgI C'CryG_G
Cuts at: 513 1065 2824 2864 2957 3582 3955 3961 4008
Size: 552 1759 40 93 625 373 6 47
Cuts at: 4008 4045 513
Size: 37 4299
Fragments arranged by size:
4299 1759 625 552 373 93 47 40
37 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2184 3123 487
Size: 683 56 958 939 5195
Fragments arranged by size:
5195 958 939 683 56
BtsI GCAGTG_nn'
Cuts at: 2285 2567 2736 4774 5347 6141 6161 2285
Size: 282 169 2038 573 794 20 3955
Fragments arranged by size:
3955 2038 794 573 282 169 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7831
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4704 4739 529
Size: 35 4140 35 3621
Fragments arranged by size:
4140 3621 35 35
DraI TTT'AAA
Cuts at: 5135 5135
Size: 7831
DraIII CAC_nnn'GTG
Cuts at: 2825 2825
Size: 7831
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3643 6838 809
Size: 507 2327 3195 1802
Fragments arranged by size:
3195 2327 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2580 3429 3528
Size: 54 1425 50 275 18 750 849 99
Cuts at: 3528 3555 3559 3585 3621 3846 3880 3958 4000
Size: 27 4 26 36 225 34 78 42
Cuts at: 4000 4201 4297 4476 4584 5127 5278 6167 7796
Size: 201 96 179 108 543 151 889 1629
Cuts at: 7796 8
Size: 43
Fragments arranged by size:
1629 1425 889 849 750 543 275 225
201 179 151 108 99 96 78 54
50 43 42 36 34 27 26 18
4
EagI C'GGCC_G
Cuts at: 1537 1812 2580 3555 3846 3880 3958 4297 5278
Size: 275 768 975 291 34 78 339 981
Cuts at: 5278 1537
Size: 4090
Fragments arranged by size:
4090 981 975 768 339 291 275 78
34
EarI CTCTTCn'nnn_
Cuts at: 1353 2226 2273 5761 1353
Size: 873 47 3488 3423
Fragments arranged by size:
3488 3423 873 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3498 5009 5072 6418 6792 6938
Size: 1064 138 1909 1511 63 1346 374 146
Cuts at: 6938 387
Size: 1280
Fragments arranged by size:
1909 1511 1346 1280 1064 374 146 138
63
EcoICRI GAG'CTC
Cuts at: 2509 2509
Size: 7831
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2430 2615 2651 2912 4443 4799 4853
Size: 393 798 185 36 261 1531 356 54
Cuts at: 4853 5030 5894 6491 7278 7546 1239
Size: 177 864 597 787 268 1524
Fragments arranged by size:
1531 1524 864 798 787 597 393 356
268 261 185 177 54 36
EcoNI CCTnn'n_nnAGG
Cuts at: 4637 4982 5012 4637
Size: 345 30 7456
Fragments arranged by size:
7456 345 30
EcoO109I rG'GnC_Cy
Cuts at: 2103 2146 2187 2436 2858 2859 3488 3712 3993
Size: 43 41 249 422 1 629 224 281
Cuts at: 3993 4362 4363 4463 4546 4701 4773 4975 2103
Size: 369 1 100 83 155 72 202 4959
Fragments arranged by size:
4959 629 422 369 281 249 224 202
155 100 83 72 43 41 1 1
EcoRI G'AATT_C
Cuts at: 5140 5518 5140
Size: 378 7453
FseI GG_CCGG'CC
Cuts at: 3561 4303 3561
Size: 742 7089
FspI TGC'GCA
Cuts at: 2159 6337 2159
Size: 4178 3653
HaeII r_GCGC'y
Cuts at: 1678 2906 3283 3539 3697 3723 3770 3872 4073
Size: 1228 377 256 158 26 47 102 201
Cuts at: 4073 4144 4259 4691 6978 1678
Size: 71 115 432 2287 2531
Fragments arranged by size:
2531 2287 1228 432 377 256 201 158
115 102 71 47 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4826 4845 4858 4877 6472
Size: 32 135 32 3254 19 13 19 1595
Cuts at: 6472 6504 6546 6578 1373
Size: 32 42 32 2626
Fragments arranged by size:
3254 2626 1595 135 42 32 32 32
32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 5175 5421 669
Size: 988 281 3237 246 3079
Fragments arranged by size:
3237 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7831
HpaI GTT'AAC
Cuts at: 5421 5421
Size: 7831
KasI G'GCGC_C
Cuts at: 2902 3693 3719 3766 3868 4255 4687 2902
Size: 791 26 47 102 387 432 6046
Fragments arranged by size:
6046 791 432 387 102 47 26
KpnI G_GTAC'C
Cuts at: 2957 5156 2957
Size: 2199 5632
MfeI C'AATT_G
Cuts at: 3895 5430 3895
Size: 1535 6296
MluI A'CGCG_T
Cuts at: 7707 7707
Size: 7831
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3022 4518 4737 6945 7129 1305
Size: 1717 1496 219 2208 184 2007
Fragments arranged by size:
2208 2007 1717 1496 219 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3530 3587 3623 4586 10
Size: 54 1425 343 1698 57 36 963 3255
Fragments arranged by size:
3255 1698 1425 963 343 57 54 36
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3748 3821 4133 5789 6148 6307
Size: 648 843 1739 73 312 1656 359 159
Cuts at: 6307 518
Size: 2042
Fragments arranged by size:
2042 1739 1656 843 648 359 312 159
73
MspA1I CmG'CkG
Cuts at: 1606 2110 2163 2281 2763 2911 2922 3737 3943
Size: 504 53 118 482 148 11 815 206
Cuts at: 3943 3963 4036 4235 4423 5104 5247 5924 7072
Size: 20 73 199 188 681 143 677 1148
Cuts at: 7072 7317 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 815 681 677 504 482 245
206 199 188 148 143 118 73 53
20 11
NaeI GCC'GGC
Cuts at: 2020 3559 4301 4355 2020
Size: 1539 742 54 5496
Fragments arranged by size:
5496 1539 742 54
NarI GG'CG_CC
Cuts at: 2903 3694 3720 3767 3869 4256 4688 2903
Size: 791 26 47 102 387 432 6046
Fragments arranged by size:
6046 791 432 387 102 47 26
NcoI C'CATG_G
Cuts at: 513 1065 3582 513
Size: 552 2517 4762
Fragments arranged by size:
4762 2517 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7831
NgoMIV G'CCGG_C
Cuts at: 2018 3557 4299 4353 2018
Size: 1539 742 54 5496
Fragments arranged by size:
5496 1539 742 54
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7831
NotI GC'GGCC_GC
Cuts at: 5278 5278
Size: 7831
NruI TCG'CGA
Cuts at: 3909 3909
Size: 7831
NspI r_CATG'y
Cuts at: 1132 1229 5189 6734 1132
Size: 97 3960 1545 2229
Fragments arranged by size:
3960 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3682 1348
Size: 2334 5497
PciI A'CATG_T
Cuts at: 6730 6730
Size: 7831
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3583 4009 4466 4871 4916 1108
Size: 2475 426 457 405 45 4023
Fragments arranged by size:
4023 2475 457 426 405 45
PfoI T'CCnGG_A
Cuts at: 2592 3315 2592
Size: 723 7108
PmeI GTTT'AAAC
Cuts at: 5135 5135
Size: 7831
PmlI CAC'GTG
Cuts at: 2899 3226 3373 4135 2899
Size: 327 147 762 6595
Fragments arranged by size:
6595 762 327 147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2600 2632 3203 3235 5900 5932 7413 7445 2600
Size: 32 571 32 2665 32 1481 32 2986
Fragments arranged by size:
2986 2665 1481 571 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2436 3993 4773 4975 2436
Size: 1557 780 202 5292
Fragments arranged by size:
5292 1557 780 202
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7831
PsiI TTA'TAA
Cuts at: 5401 5401
Size: 7831
PspOMI G'GGCC_C
Cuts at: 2146 2719 2858 3144 3712 4362 2146
Size: 573 139 286 568 650 5615
Fragments arranged by size:
5615 650 573 568 286 139
PstI C_TGCA'G
Cuts at: 830 2251 3732 4035 4344 4971 4983 5055 5183
Size: 1421 1481 303 309 627 12 72 128
Cuts at: 5183 830
Size: 3478
Fragments arranged by size:
3478 1481 1421 627 309 303 128 72
12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7831
PvuII CAG'CTG
Cuts at: 2911 2922 3943 4036 4423 5104 2911
Size: 11 1021 93 387 681 5638
Fragments arranged by size:
5638 1021 681 387 93 11
SacI G_AGCT'C
Cuts at: 2511 2511
Size: 7831
SacII CC_GC'GG
Cuts at: 3964 3964
Size: 7831
SalI G'TCGA_C
Cuts at: 1936 5173 1936
Size: 3237 4594
SanDI GG'GwC_CC
Cuts at: 2436 3993 4773 2436
Size: 1557 780 5494
Fragments arranged by size:
5494 1557 780
SapI GCTCTTCn'nnn_
Cuts at: 2273 2273
Size: 7831
SbfI CC_TGCA'GG
Cuts at: 5183 5183
Size: 7831
ScaI AGT'ACT
Cuts at: 6079 7791 6079
Size: 1712 6119
SfcI C'TryA_G
Cuts at: 826 1045 2247 2623 3421 3728 4031 4340 4967
Size: 219 1202 376 798 307 303 309 627
Cuts at: 4967 4979 5051 5179 6314 6995 7186 826
Size: 12 72 128 1135 681 191 1471
Fragments arranged by size:
1471 1202 1135 798 681 627 376 309
307 303 219 191 128 72 12
SfoI GGC'GCC
Cuts at: 2904 3695 3721 3768 3870 4257 4689 2904
Size: 791 26 47 102 387 432 6046
Fragments arranged by size:
6046 791 432 387 102 47 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7831
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7831
SmaI CCC'GGG
Cuts at: 1872 3772 4177 5158 1872
Size: 1900 405 981 4545
Fragments arranged by size:
4545 1900 981 405
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2336 5935 6836 7098 820
Size: 197 926 393 3599 901 262 1553
Fragments arranged by size:
3599 1553 926 901 393 262 197
SnaBI TAC'GTA
Cuts at: 493 5622 493
Size: 5129 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7831
SphI G_CATG'C
Cuts at: 1132 5189 1132
Size: 4057 3774
SspI AAT'ATT
Cuts at: 5 52 5755 5
Size: 47 5703 2081
Fragments arranged by size:
5703 2081 47
StuI AGG'CCT
Cuts at: 1517 2532 1517
Size: 1015 6816
StyI C'CwwG_G
Cuts at: 513 1065 2879 2949 3582 4260 5270 513
Size: 552 1814 70 633 678 1010 3074
Fragments arranged by size:
3074 1814 1010 678 633 552 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2525 2636 2962 3458 3674 5128 5858
Size: 1255 945 111 326 496 216 1454 730
Cuts at: 5858 5875 6028 6213 6658 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1454 1255 945 730 496 445 326
216 185 153 111 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3018 3054 4326
Size: 274 80 33 51 1443 1041 36 1272
Cuts at: 4326 6077 7789 96
Size: 1751 1712 138
Fragments arranged by size:
1751 1712 1443 1272 1041 274 138 80
51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2809 2841 4772 4804 4934 4966 5070 5102 7791
Size: 32 1931 32 130 32 104 32 2689
Cuts at: 7791 7823 2809
Size: 32 2817
Fragments arranged by size:
2817 2689 1931 130 104 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7831
XbaI T'CTAG_A
Cuts at: 5167 5167
Size: 7831
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3424 3646 5122 3424
Size: 222 1476 6133
Fragments arranged by size:
6133 1476 222
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7831
XmaI C'CCGG_G
Cuts at: 1870 3770 4175 5156 1870
Size: 1900 405 981 4545
Fragments arranged by size:
4545 1900 981 405
XmnI GAAnn'nnTTC
Cuts at: 2696 5960 2696
Size: 3264 4567
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5639 276
Size: 53 83 186 936 4105 2468
Fragments arranged by size:
4105 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
FalI FspAI NsiI PacI PspXI PsrI RsrII SexAI
SfiI SrfI SwaI