Restriction map for
pFN21AB4848
(Circular) (Six-base) MAPSORT of: pFN21AB4848.seq Check: 2806 from: 1 to: 5962
pFN21AB4848 5962 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 1937
Size: 1368 4594
Acc65I G'GTAC_C
Cuts at: 2220 3283 2220
Size: 1063 4899
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4025 5409 5677 1632
Size: 2393 1384 268 1917
Fragments arranged by size:
2393 1917 1384 268
AfeI AGC'GCT
Cuts at: 2159 2159
Size: 5962
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 4861 5838 1203
Size: 3658 977 1327
Fragments arranged by size:
3658 1327 977
AgeI A'CCGG_T
Cuts at: 2217 4793 2217
Size: 2576 3386
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 2246 1166
Size: 1080 4882
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AlwNI CAG_nnn'CTG
Cuts at: 3090 3197 3705 5277 3090
Size: 107 508 1572 3775
Fragments arranged by size:
3775 1572 508 107
ApaLI G'TGCA_C
Cuts at: 2189 4019 5175 2189
Size: 1830 1156 2976
Fragments arranged by size:
2976 1830 1156
ApoI r'AATT_y
Cuts at: 1493 3271 3497 3649 5758 1493
Size: 1778 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 3287 1870
Size: 73 1344 4545
Fragments arranged by size:
4545 1344 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2212 2245 2967 3000 2212
Size: 33 722 33 5174
Fragments arranged by size:
5174 722 33 33
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2220 3283 4738 618
Size: 325 217 627 433 1063 1455 1842
Fragments arranged by size:
1842 1455 1063 627 433 325 217
BanII G_rGCy'C
Cuts at: 1391 2531 2858 2966 3222 3257 3281 1391
Size: 1140 327 108 256 35 24 4072
Fragments arranged by size:
4072 1140 327 256 108 35 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2272 928
Size: 971 373 4618
Fragments arranged by size:
4618 971 373
BbvCI CC'TCA_GC
Cuts at: 3174 3174
Size: 5962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2968 3854 5064 2968
Size: 886 1210 3866
Fragments arranged by size:
3866 1210 886
BclI T'GATC_A
Cuts at: 1548 2826 1548
Size: 1278 4684
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3226 4573 136
Size: 107 122 71 631 605 1554 1347 1525
Fragments arranged by size:
1554 1525 1347 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5957 5957
Size: 5962
BlpI GC'TnA_GC
Cuts at: 2880 3198 3379 2880
Size: 318 181 5463
Fragments arranged by size:
5463 318 181
Bme1580I G_kGCm'C
Cuts at: 1790 2193 4023 5179 1790
Size: 403 1830 1156 2573
Fragments arranged by size:
2573 1830 1156 403
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3693 4651 442
Size: 416 53 83 399 24 2276 958 1753
Fragments arranged by size:
2276 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3119 4622 1239
Size: 1880 1503 2579
Fragments arranged by size:
2579 1880 1503
Bpu10I CC'TnA_GC
Cuts at: 3174 3174
Size: 5962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2240 4087 4952 5250 2240
Size: 1847 865 298 2952
Fragments arranged by size:
2952 1847 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2204 2432 2591 3065 4625 882
Size: 617 705 228 159 474 1560 2219
Fragments arranged by size:
2219 1560 705 617 474 228 159
BsaAI yAC'GTr
Cuts at: 493 1204 3753 493
Size: 711 2549 2702
Fragments arranged by size:
2702 2549 711
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3769 4151 4786 275
Size: 53 83 186 936 2236 382 635 1451
Fragments arranged by size:
2236 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2217 4395 4793 5067 5214 5625 2217
Size: 2178 398 274 147 411 2554
Fragments arranged by size:
2554 2178 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5737
Fragments arranged by size:
5737 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2495 2811 3122 1186
Size: 1309 316 311 4026
Fragments arranged by size:
4026 1309 316 311
BseYI C'CCAG_C
Cuts at: 3083 3146 3160 3229 5165 5661 3083
Size: 63 14 69 1936 496 3384
Fragments arranged by size:
3384 1936 496 69 63 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5389
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2803 3412 4173 5201 1540
Size: 275 186 802 609 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 609 275 186
BsiHKAI G_wGCw'C
Cuts at: 2193 2966 2988 4023 4108 5179 2193
Size: 773 22 1035 85 1071 2976
Fragments arranged by size:
2976 1071 1035 773 85 22
BsiWI C'GTAC_G
Cuts at: 2120 2120
Size: 5962
BsmI GAATG_Cn'
Cuts at: 2029 3473 3566 2029
Size: 1444 93 4425
Fragments arranged by size:
4425 1444 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2531 2858 2966 2988 3222 3257
Size: 399 403 338 327 108 22 234 35
Cuts at: 3257 3281 4023 4108 5179 1391
Size: 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 403 399 338 327 234
108 85 35 24 22
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 3851 5581 3851
Size: 1730 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2957 3317 844
Size: 2113 360 3489
Fragments arranged by size:
3489 2113 360
BsrBI CCG'CTC
Cuts at: 1394 1823 2175 2594 3849 1394
Size: 429 352 419 1255 3507
Fragments arranged by size:
3507 1255 429 419 352
BsrDI GCAATG_nn'
Cuts at: 65 1248 2106 2334 4457 4631 65
Size: 1183 858 228 2123 174 1396
Fragments arranged by size:
2123 1396 1183 858 228 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2217 3126 4606 4793 1700
Size: 110 407 909 1480 187 2869
Fragments arranged by size:
2869 1480 909 407 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 2837 4022 5034 2837
Size: 1185 1012 3765
Fragments arranged by size:
3765 1185 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2949 794
Size: 2155 3807
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 2351 1184
Size: 1167 4795
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 3103 3229 2248
Size: 855 126 4981
Fragments arranged by size:
4981 855 126
BstYI r'GATC_y
Cuts at: 1972 3292 3659 4044 4061 5513 5599 5611 5957
Size: 1320 367 385 17 1452 86 12 346
Cuts at: 5957 1972
Size: 1977
Fragments arranged by size:
1977 1452 1320 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2194 2286 513
Size: 552 939 190 92 4189
Fragments arranged by size:
4189 939 552 190 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 3155 3478 4272 4292 3155
Size: 323 794 20 4825
Fragments arranged by size:
4825 794 323 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DraIII CAC_nnn'GTG
Cuts at: 2692 2692
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 3409 4298
Size: 54 1425 50 275 18 303 1276 889
Cuts at: 4298 5927 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1276 889 303 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3409 1537
Size: 275 1597 4090
Fragments arranged by size:
4090 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 2240 2582 2813 2846 2889 3110 3892
Size: 812 75 342 231 33 43 221 782
Cuts at: 3892 1353
Size: 3423
Fragments arranged by size:
3423 812 782 342 231 221 75 43
33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2964 2964
Size: 5962
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3119 4025 4622 5409 5677 1239
Size: 393 1487 906 597 787 268 1524
Fragments arranged by size:
1524 1487 906 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2065 2065
Size: 5962
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2281 2281
Size: 5962
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 2161 2266 5109 1678
Size: 483 105 2843 2531
Fragments arranged by size:
2843 2531 483 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4603 4635 4677 4709 1373
Size: 32 135 32 3031 32 42 32 2626
Fragments arranged by size:
3031 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 2224 3287 2224
Size: 1063 4899
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2637 5076 5260 1305
Size: 1332 2439 184 2007
Fragments arranged by size:
2439 2007 1332 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4140
Fragments arranged by size:
4140 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 2630 2777 2942 3920 4279 4438
Size: 648 1080 384 147 165 978 359 159
Cuts at: 4438 518
Size: 2042
Fragments arranged by size:
2042 1080 978 648 384 359 165 159
147
MspA1I CmG'CkG
Cuts at: 1606 2884 3087 3378 4055 5203 5448 1606
Size: 1278 203 291 677 1148 245 2120
Fragments arranged by size:
2120 1278 1148 677 291 245 203
NaeI GCC'GGC
Cuts at: 3128 3128
Size: 5962
NcoI C'CATG_G
Cuts at: 513 1065 2004 2286 513
Size: 552 939 282 4189
Fragments arranged by size:
4189 939 552 282
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NgoMIV G'CCGG_C
Cuts at: 3126 3126
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 2563 3320 4865 1132
Size: 97 1334 757 1545 2229
Fragments arranged by size:
2229 1545 1334 757 97
PasI CC'CwG_GG
Cuts at: 1348 2061 1348
Size: 713 5249
PciI A'CATG_T
Cuts at: 4861 4861
Size: 5962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2041 2692 1108
Size: 933 651 4378
Fragments arranged by size:
4378 933 651
PfoI T'CCnGG_A
Cuts at: 2205 2205
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2732 2764 4031 4063 5544 5576 2732
Size: 32 1267 32 1481 32 3118
Fragments arranged by size:
3118 1481 1267 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2065 2065
Size: 5962
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PstI C_TGCA'G
Cuts at: 830 3314 830
Size: 2484 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
PvuII CAG'CTG
Cuts at: 2884 3087 2884
Size: 203 5759
SacI G_AGCT'C
Cuts at: 2966 2966
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SanDI GG'GwC_CC
Cuts at: 2065 2065
Size: 5962
SapI GCTCTTCn'nnn_
Cuts at: 2165 2846 2165
Size: 681 5281
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 3310 4445 5126 5317 826
Size: 219 2265 1135 681 191 1471
Fragments arranged by size:
2265 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 3289 1872
Size: 1417 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2255 4066 4967 5229 820
Size: 197 926 312 1811 901 262 1553
Fragments arranged by size:
1811 1553 926 901 312 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 2699 1517
Size: 1182 4780
StyI C'CwwG_G
Cuts at: 513 1065 2004 2286 3018 3096 3222 3401 513
Size: 552 939 282 732 78 126 179 3074
Fragments arranged by size:
3074 939 732 552 282 179 126 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2456 3097 3221 3989 4006 4159 4344
Size: 1255 876 641 124 768 17 153 185
Cuts at: 4344 4789 325
Size: 445 1498
Fragments arranged by size:
1498 1255 876 768 641 445 185 153
124 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4208 5920 96
Size: 274 80 33 51 1443 2231 1712 138
Fragments arranged by size:
2231 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2243 2275 2374 2406 5922 5954 2243
Size: 32 99 32 3516 32 2251
Fragments arranged by size:
3516 2251 99 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 2917 3298 2917
Size: 381 5581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3256 3256
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 3287 1870
Size: 1417 4545
XmnI GAAnn'nnTTC
Cuts at: 4091 4091
Size: 5962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SexAI SfiI SfoI SrfI SwaI