Restriction map for
pFN21AB4835
(Circular) (Six-base) MAPSORT of: pFN21AB4835.seq Check: 8137 from: 1 to: 6139
pFN21AB4835 6139 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2688 2749 3144 2688
Size: 61 395 5683
Fragments arranged by size:
5683 395 61
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3949 278
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3482 1937
Size: 1545 4594
Acc65I G'GTAC_C
Cuts at: 2531 3460 2531
Size: 929 5210
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2531 2856 3344 4202 5586 5854 1632
Size: 899 325 488 858 1384 268 1917
Fragments arranged by size:
1917 1384 899 858 488 325 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5942
AflIII A'CryG_T
Cuts at: 1203 2730 5038 6015 1203
Size: 1527 2308 977 1327
Fragments arranged by size:
2308 1527 1327 977
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 4868 4868
Size: 6139
AleI CACnn'nnGTG
Cuts at: 1166 2051 1166
Size: 885 5254
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6139
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 6107
AlwNI CAG_nnn'CTG
Cuts at: 3232 3882 5454 3232
Size: 650 1572 3917
Fragments arranged by size:
3917 1572 650
ApaI G_GGCC'C
Cuts at: 2169 2169
Size: 6139
ApaLI G'TGCA_C
Cuts at: 4196 5352 4196
Size: 1156 4983
ApoI r'AATT_y
Cuts at: 1493 2408 3448 3674 3826 5935 1493
Size: 915 1040 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 915 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4693 160
Size: 872 3661 1606
Fragments arranged by size:
3661 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 2358 2662 3201 3464 1870
Size: 73 415 304 539 263 4545
Fragments arranged by size:
4545 539 415 304 263 73
BamHI G'GATC_C
Cuts at: 3469 3836 3469
Size: 367 5772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2531 3132 3460 4915
Size: 325 217 627 255 489 601 328 1455
Cuts at: 4915 618
Size: 1842
Fragments arranged by size:
1842 1455 627 601 489 328 325 255
217
BanII G_rGCy'C
Cuts at: 1391 2169 2358 2663 3208 3458 1391
Size: 778 189 305 545 250 4072
Fragments arranged by size:
4072 778 545 305 250 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5168
BbvCI CC'TCA_GC
Cuts at: 2069 2069
Size: 6139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2204 2238 2579 2613 4330 4364 1903
Size: 34 267 34 341 34 1717 34 3678
Fragments arranged by size:
3678 1717 341 267 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4031 5241 4031
Size: 1210 4929
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6139
BfrBI ATG'CAT
Cuts at: 2284 2284
Size: 6139
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2173 4750 136
Size: 107 122 71 631 605 501 2577 1525
Fragments arranged by size:
2577 1525 631 605 501 122 107 71
BglII A'GATC_T
Cuts at: 3022 6134 3022
Size: 3112 3027
BlpI GC'TnA_GC
Cuts at: 3556 3556
Size: 6139
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2122 2169 3195 4200 5356 1790
Size: 255 77 47 1026 1005 1156 2573
Fragments arranged by size:
2573 1156 1026 1005 255 77 47
BmgBI CAC'GTC
Cuts at: 1318 3319 1318
Size: 2001 4138
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2906 3870 4828
Size: 416 53 83 399 24 1489 964 958
Cuts at: 4828 442
Size: 1753
Fragments arranged by size:
1753 1489 964 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2880 3053 3245 4799 1239
Size: 1641 173 192 1554 2579
Fragments arranged by size:
2579 1641 1554 192 173
Bpu10I CC'TnA_GC
Cuts at: 2069 2069
Size: 6139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4264 5129 5427 4264
Size: 865 298 4976
Fragments arranged by size:
4976 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4802 882
Size: 617 3303 2219
Fragments arranged by size:
3303 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2382 3196 3930 493
Size: 711 1178 814 734 2702
Fragments arranged by size:
2702 1178 814 734 711
BsaBI GATnn'nnATC
Cuts at: 6133 6133
Size: 6139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3946 4328 4963 275
Size: 53 83 186 936 2413 382 635 1451
Fragments arranged by size:
2413 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2247 3411 4572 4970 5244 5391 5802 2247
Size: 1164 1161 398 274 147 411 2584
Fragments arranged by size:
2584 1164 1161 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2229 2259 2650 2680 3221
Size: 30 165 30 826 30 391 30 541
Cuts at: 3221 3251 1178
Size: 30 4066
Fragments arranged by size:
4066 826 541 391 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2671 3160 3163 1186
Size: 1485 489 3 4162
Fragments arranged by size:
4162 1485 489 3
BseYI C'CCAG_C
Cuts at: 2241 2427 2607 3121 5342 5838 2241
Size: 186 180 514 2221 496 2542
Fragments arranged by size:
2542 2221 514 496 186 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2871 3101 3218 3356 1163
Size: 573 1135 230 117 138 3946
Fragments arranged by size:
3946 1135 573 230 138 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2018 2148 3589 4350 5378 1540
Size: 275 186 17 130 1441 761 1028 2301
Fragments arranged by size:
2301 1441 1028 761 275 186 130 17
BsiHKAI G_wGCw'C
Cuts at: 2663 4200 4285 5356 2663
Size: 1537 85 1071 3446
Fragments arranged by size:
3446 1537 1071 85
BsmI GAATG_Cn'
Cuts at: 2602 3650 3743 2602
Size: 1048 93 4998
Fragments arranged by size:
4998 1048 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2122 2169 2358 2663 3195 3208
Size: 399 255 77 47 189 305 532 13
Cuts at: 3208 3458 4200 4285 5356 1391
Size: 250 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 532 399 305 255 250
189 85 77 47 13
BspEI T'CCGG_A
Cuts at: 2247 3411 5802 2247
Size: 1164 2391 2584
Fragments arranged by size:
2584 2391 1164
BspHI T'CATG_A
Cuts at: 4028 5758 4028
Size: 1730 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2688 2749 3144 3240 3494 844
Size: 1844 61 395 96 254 3489
Fragments arranged by size:
3489 1844 395 254 96 61
BsrBI CCG'CTC
Cuts at: 1394 1823 4026 1394
Size: 429 2203 3507
Fragments arranged by size:
3507 2203 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2983 4634 4808 65
Size: 1183 1735 1651 174 1396
Fragments arranged by size:
1735 1651 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2528 4783 4970 1700
Size: 110 718 2255 187 2869
Fragments arranged by size:
2869 2255 718 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6139
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6139
BssSI C'ACGA_G
Cuts at: 2213 4199 5211 2213
Size: 1986 1012 3141
Fragments arranged by size:
3141 1986 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3241 3334 794
Size: 2447 93 3599
Fragments arranged by size:
3599 2447 93
BstBI TT'CG_AA
Cuts at: 4943 4943
Size: 6139
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6139
BstXI CCAn_nnnn'nTGG
Cuts at: 2285 2285
Size: 6139
BstYI r'GATC_y
Cuts at: 1972 3022 3469 3836 4221 4238 5690 5776 5788
Size: 1050 447 367 385 17 1452 86 12
Cuts at: 5788 6134 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1050 447 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2454 513
Size: 552 939 450 4198
Fragments arranged by size:
4198 939 552 450
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2677 487
Size: 683 56 1451 3949
Fragments arranged by size:
3949 1451 683 56
BtsI GCAGTG_nn'
Cuts at: 2109 2713 3298 3655 4449 4469 2109
Size: 604 585 357 794 20 3779
Fragments arranged by size:
3779 794 604 585 357 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6104
DraI TTT'AAA
Cuts at: 3443 3443
Size: 6139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2015 2160 2392
Size: 54 1425 50 275 18 185 145 232
Cuts at: 2392 2451 2955 2985 3586 4475 6104 8
Size: 59 504 30 601 889 1629 43
Fragments arranged by size:
1629 1425 889 601 504 275 232 185
145 59 54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2015 3586 1537
Size: 275 203 1571 4090
Fragments arranged by size:
4090 1571 275 203
EarI CTCTTCn'nnn_
Cuts at: 1353 4069 1353
Size: 2716 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4726 5100 5246 387
Size: 1064 138 3137 374 146 1280
Fragments arranged by size:
3137 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2661 2661
Size: 6139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2531 2856 2880 3053 3245 3344 4202
Size: 393 899 325 24 173 192 99 858
Cuts at: 4202 4799 5586 5854 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 899 858 787 597 393 325 268
192 173 99 24
EcoO109I rG'GnC_Cy
Cuts at: 2166 2940 2166
Size: 774 5365
EcoRI G'AATT_C
Cuts at: 2408 3448 3826 2408
Size: 1040 378 4721
Fragments arranged by size:
4721 1040 378
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 5286 1678
Size: 3608 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 2229 2261 3007
Size: 32 135 32 503 32 122 32 746
Cuts at: 3007 3039 3190 3222 4780 4812 4854 4886 1373
Size: 32 151 32 1558 32 42 32 2626
Fragments arranged by size:
2626 1558 746 503 151 135 122 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3483 3729 669
Size: 988 281 1545 246 3079
Fragments arranged by size:
3079 1545 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6139
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KpnI G_GTAC'C
Cuts at: 2535 3464 2535
Size: 929 5210
MfeI C'AATT_G
Cuts at: 3738 3738
Size: 6139
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2613 3201 5253 5437 1305
Size: 1308 588 2052 184 2007
Fragments arranged by size:
2052 2007 1308 588 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2453 2987 10
Size: 54 1425 343 621 534 3162
Fragments arranged by size:
3162 1425 621 534 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2051 2267 2445 2544 4097 4456 4615
Size: 648 885 216 178 99 1553 359 159
Cuts at: 4615 518
Size: 2042
Fragments arranged by size:
2042 1553 885 648 359 216 178 159
99
MspA1I CmG'CkG
Cuts at: 1606 2607 2854 3077 3148 3386 3555 4232 5380
Size: 1001 247 223 71 238 169 677 1148
Cuts at: 5380 5625 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1001 677 247 245 238 223
169 71
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4648
Fragments arranged by size:
4648 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6139
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6139
NotI GC'GGCC_GC
Cuts at: 3586 3586
Size: 6139
NsiI A_TGCA'T
Cuts at: 2286 2286
Size: 6139
NspI r_CATG'y
Cuts at: 1132 1229 2734 3004 3497 5042 1132
Size: 97 1505 270 493 1545 2229
Fragments arranged by size:
2229 1545 1505 493 270 97
PasI CC'CwG_GG
Cuts at: 1348 2170 3187 1348
Size: 822 1017 4300
Fragments arranged by size:
4300 1017 822
PciI A'CATG_T
Cuts at: 2730 5038 2730
Size: 2308 3831
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2481 3004 1108
Size: 1373 523 4243
Fragments arranged by size:
4243 1373 523
PfoI T'CCnGG_A
Cuts at: 3261 3308 3261
Size: 47 6092
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PmlI CAC'GTG
Cuts at: 2382 3196 2382
Size: 814 5325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3207 3221 3239 3253 4208 4240 5721 5753 3207
Size: 14 18 14 955 32 1481 32 3593
Fragments arranged by size:
3593 1481 955 32 32 18 14 14
PpuMI rG'GwC_Cy
Cuts at: 2940 2940
Size: 6139
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6139
PsiI TTA'TAA
Cuts at: 3709 3709
Size: 6139
PspOMI G'GGCC_C
Cuts at: 2165 2165
Size: 6139
PstI C_TGCA'G
Cuts at: 830 3491 830
Size: 2661 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
PvuII CAG'CTG
Cuts at: 2607 2854 3077 3148 3386 2607
Size: 247 223 71 238 5360
Fragments arranged by size:
5360 247 238 223 71
SacI G_AGCT'C
Cuts at: 2663 2663
Size: 6139
SalI G'TCGA_C
Cuts at: 1936 3481 1936
Size: 1545 4594
SbfI CC_TGCA'GG
Cuts at: 3491 3491
Size: 6139
ScaI AGT'ACT
Cuts at: 4387 6099 4387
Size: 1712 4427
SexAI A'CCwGG_T
Cuts at: 2373 2373
Size: 6139
SfcI C'TryA_G
Cuts at: 826 1045 3487 4622 5303 5494 826
Size: 219 2442 1135 681 191 1471
Fragments arranged by size:
2442 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6139
SmaI CCC'GGG
Cuts at: 1872 3203 3466 1872
Size: 1331 263 4545
Fragments arranged by size:
4545 1331 263
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4243 5144 5406 820
Size: 197 926 2300 901 262 1553
Fragments arranged by size:
2300 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3930 493
Size: 3437 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6139
SphI G_CATG'C
Cuts at: 1132 3497 1132
Size: 2365 3774
SspI AAT'ATT
Cuts at: 5 52 4063 5
Size: 47 4011 2081
Fragments arranged by size:
4011 2081 47
StuI AGG'CCT
Cuts at: 1517 2789 3030 1517
Size: 1272 241 4626
Fragments arranged by size:
4626 1272 241
StyI C'CwwG_G
Cuts at: 513 1065 2004 2311 2772 3578 513
Size: 552 939 307 461 806 3074
Fragments arranged by size:
3074 939 806 552 461 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2765 4166 4183 4336 4521 4966 325
Size: 1255 1185 1401 17 153 185 445 1498
Fragments arranged by size:
1498 1401 1255 1185 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4385 6097 96
Size: 274 80 33 51 1443 2408 1712 138
Fragments arranged by size:
2408 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6099 6131 6099
Size: 32 6107
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6139
XbaI T'CTAG_A
Cuts at: 3475 3475
Size: 6139
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6139
XmaI C'CCGG_G
Cuts at: 1870 3201 3464 1870
Size: 1331 263 4545
Fragments arranged by size:
4545 1331 263
XmnI GAAnn'nnTTC
Cuts at: 4268 4268
Size: 6139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3947 276
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI