Restriction map for
pFN21AB4822
(Circular) (Six-base) MAPSORT of: pFN21AB4822.seq Check: 8698 from: 1 to: 6058
pFN21AB4822 6058 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3868 278
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3401 1937
Size: 1464 4594
Acc65I G'GTAC_C
Cuts at: 3379 3379
Size: 6058
AclI AA'CG_TT
Cuts at: 1563 4185 4558 1563
Size: 2622 373 3063
Fragments arranged by size:
3063 2622 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4121 5505 5773 1632
Size: 2489 1384 268 1917
Fragments arranged by size:
2489 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 4957 5934 1203
Size: 3754 977 1327
Fragments arranged by size:
3754 1327 977
AgeI A'CCGG_T
Cuts at: 4889 4889
Size: 6058
AhdI GACnn_n'nnGTC
Cuts at: 4787 4787
Size: 6058
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3132 1184
Size: 1948 4110
AlwNI CAG_nnn'CTG
Cuts at: 2086 2875 3801 5373 2086
Size: 789 926 1572 2771
Fragments arranged by size:
2771 1572 926 789
ApaI G_GGCC'C
Cuts at: 3021 3021
Size: 6058
ApaLI G'TGCA_C
Cuts at: 4115 5271 4115
Size: 1156 4902
ApoI r'AATT_y
Cuts at: 1493 3367 3593 3745 5854 1493
Size: 1874 226 152 2109 1697
Fragments arranged by size:
2109 1874 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4612 160
Size: 872 3580 1606
Fragments arranged by size:
3580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 2816 3383 1870
Size: 73 174 699 567 4545
Fragments arranged by size:
4545 699 567 174 73
BamHI G'GATC_C
Cuts at: 3388 3755 3388
Size: 367 5691
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3379 4834 618
Size: 325 217 627 1592 1455 1842
Fragments arranged by size:
1842 1592 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2976 3021 3377 1391
Size: 1585 45 356 4072
Fragments arranged by size:
4072 1585 356 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2291 928
Size: 971 392 4695
Fragments arranged by size:
4695 971 392
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4249 4283 1903
Size: 34 2312 34 3678
Fragments arranged by size:
3678 2312 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3950 5160 3950
Size: 1210 4848
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6058
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2820 4669 136
Size: 107 122 71 631 605 1148 1849 1525
Fragments arranged by size:
1849 1525 1148 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3329 6053 3329
Size: 2724 3334
BlpI GC'TnA_GC
Cuts at: 2927 3475 2927
Size: 548 5510
Bme1580I G_kGCm'C
Cuts at: 1790 2230 2677 3021 4119 5275 1790
Size: 440 447 344 1098 1156 2573
Fragments arranged by size:
2573 1156 1098 447 440 344
BmgBI CAC'GTC
Cuts at: 1318 2808 1318
Size: 1490 4568
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3019 3789 4747
Size: 416 53 83 399 24 1602 770 958
Cuts at: 4747 442
Size: 1753
Fragments arranged by size:
1753 1602 958 770 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3233 1056
Size: 2177 3881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2447 2557 2957 3074 4718 1239
Size: 794 414 110 400 117 1644 2579
Fragments arranged by size:
2579 1644 794 414 400 117 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4183 5048 5346 4183
Size: 865 298 4895
Fragments arranged by size:
4895 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3054 4721 882
Size: 617 1555 1667 2219
Fragments arranged by size:
2219 1667 1555 617
BsaAI yAC'GTr
Cuts at: 493 1204 2847 3217 3849 493
Size: 711 1643 370 632 2702
Fragments arranged by size:
2702 1643 711 632 370
BsaBI GATnn'nnATC
Cuts at: 6052 6052
Size: 6058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2205 2853 3865 4247
Size: 53 83 186 936 672 648 1012 382
Cuts at: 4247 4882 275
Size: 635 1451
Fragments arranged by size:
1451 1012 936 672 648 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4491 4889 5163 5310 5721 4491
Size: 398 274 147 411 4828
Fragments arranged by size:
4828 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3116 3146 1178
Size: 30 165 30 1713 30 4090
Fragments arranged by size:
4090 1713 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2213 3185 1186
Size: 967 60 972 4059
Fragments arranged by size:
4059 972 967 60
BseYI C'CCAG_C
Cuts at: 2488 2493 2626 5261 5757 2488
Size: 5 133 2635 496 2789
Fragments arranged by size:
2789 2635 496 133 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2744 1163
Size: 573 1008 4477
Fragments arranged by size:
4477 1008 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2026 2530 3040 3130 3508 4269
Size: 275 186 25 504 510 90 378 761
Cuts at: 4269 5297 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 510 504 378 275 186
90 25
BsiHKAI G_wGCw'C
Cuts at: 4119 4204 5275 4119
Size: 85 1071 4902
Fragments arranged by size:
4902 1071 85
BsmI GAATG_Cn'
Cuts at: 3569 3662 3569
Size: 93 5965
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2230 2677 2976 3021 3377 4119 4204
Size: 399 440 447 299 45 356 742 85
Cuts at: 4204 5275 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 447 440 399 356 299
85 45
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 3947 5677 3947
Size: 1730 4328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2488 3077 3413 844
Size: 1644 589 336 3489
Fragments arranged by size:
3489 1644 589 336
BsrBI CCG'CTC
Cuts at: 1394 1823 2341 3132 3945 1394
Size: 429 518 791 813 3507
Fragments arranged by size:
3507 813 791 518 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2323 4553 4727 65
Size: 1183 1075 2230 174 1396
Fragments arranged by size:
2230 1396 1183 1075 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2754 3039 4702 4889 1700
Size: 110 944 285 1663 187 2869
Fragments arranged by size:
2869 1663 944 285 187 110
BsrGI T'GTAC_A
Cuts at: 96 2376 96
Size: 2280 3778
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6058
BssSI C'ACGA_G
Cuts at: 2789 4118 5130 2789
Size: 1329 1012 3717
Fragments arranged by size:
3717 1329 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6058
BstBI TT'CG_AA
Cuts at: 4862 4862
Size: 6058
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6058
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2863 3052 2011
Size: 852 189 5017
Fragments arranged by size:
5017 852 189
BstYI r'GATC_y
Cuts at: 1972 2829 3003 3329 3388 3755 4140 4157 5609
Size: 857 174 326 59 367 385 17 1452
Cuts at: 5609 5695 5707 6053 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 857 385 367 346 326 174
86 59 17 12
Bsu36I CC'TnA_GG
Cuts at: 2391 3278 2391
Size: 887 5171
BtgI C'CryG_G
Cuts at: 513 1065 2605 2836 3124 513
Size: 552 1540 231 288 3447
Fragments arranged by size:
3447 1540 552 288 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5319
Fragments arranged by size:
5319 683 56
BtsI GCAGTG_nn'
Cuts at: 3574 4368 4388 3574
Size: 794 20 5244
Fragments arranged by size:
5244 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6023
DraI TTT'AAA
Cuts at: 2236 3362 2236
Size: 1126 4932
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5065 809
Size: 507 3749 1802
Fragments arranged by size:
3749 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2023 2608 2899
Size: 54 1425 50 275 18 193 585 291
Cuts at: 2899 3127 3505 4394 6023 8
Size: 228 378 889 1629 43
Fragments arranged by size:
1629 1425 889 585 378 291 275 228
193 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2023 3127 3505 1537
Size: 275 211 1104 378 4090
Fragments arranged by size:
4090 1104 378 275 211
EarI CTCTTCn'nnn_
Cuts at: 1353 2138 2219 3988 1353
Size: 785 81 1769 3423
Fragments arranged by size:
3423 1769 785 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2046 2066 3326 4645 5019 5165
Size: 1064 138 457 20 1260 1319 374 146
Cuts at: 5165 387
Size: 1280
Fragments arranged by size:
1319 1280 1260 1064 457 374 146 138
20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2447 2557 2957 3074 4121 4718
Size: 393 401 414 110 400 117 1047 597
Cuts at: 4718 5505 5773 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1047 787 597 414 401 400 393
268 117 110
EcoNI CCTnn'n_nnAGG
Cuts at: 2792 2867 2792
Size: 75 5983
EcoO109I rG'GnC_Cy
Cuts at: 2431 2872 3018 2431
Size: 441 146 5471
Fragments arranged by size:
5471 441 146
EcoRI G'AATT_C
Cuts at: 3367 3745 3367
Size: 378 5680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 6058
FspI TGC'GCA
Cuts at: 4564 4564
Size: 6058
HaeII r_GCGC'y
Cuts at: 1678 3029 5205 1678
Size: 1351 2176 2531
Fragments arranged by size:
2531 2176 1351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2241 2273 4699 4731 4773
Size: 32 135 32 669 32 2426 32 42
Cuts at: 4773 4805 1373
Size: 32 2626
Fragments arranged by size:
2626 2426 669 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3402 3648 669
Size: 988 281 1464 246 3079
Fragments arranged by size:
3079 1464 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6058
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KpnI G_GTAC'C
Cuts at: 3383 3383
Size: 6058
MfeI C'AATT_G
Cuts at: 3657 3657
Size: 6058
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5172 5356 1305
Size: 3867 184 2007
Fragments arranged by size:
3867 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2901 10
Size: 54 1425 343 1069 3167
Fragments arranged by size:
3167 1425 1069 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2847 4016 4375 4534 518
Size: 648 1681 1169 359 159 2042
Fragments arranged by size:
2042 1681 1169 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2106 2350 2607 3474 4151 5299 5544 1606
Size: 500 244 257 867 677 1148 245 2120
Fragments arranged by size:
2120 1148 867 677 500 257 245 244
NaeI GCC'GGC
Cuts at: 2756 2756
Size: 6058
NcoI C'CATG_G
Cuts at: 513 1065 2836 513
Size: 552 1771 3735
Fragments arranged by size:
3735 1771 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6058
NgoMIV G'CCGG_C
Cuts at: 2754 2754
Size: 6058
NheI G'CTAG_C
Cuts at: 1052 3229 1052
Size: 2177 3881
NotI GC'GGCC_GC
Cuts at: 3505 3505
Size: 6058
NspI r_CATG'y
Cuts at: 1132 1229 3416 4961 1132
Size: 97 2187 1545 2229
Fragments arranged by size:
2229 2187 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6058
PciI A'CATG_T
Cuts at: 4957 4957
Size: 6058
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6058
PfoI T'CCnGG_A
Cuts at: 2550 2695 2550
Size: 145 5913
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 5640 5672 4127
Size: 32 1481 32 4513
Fragments arranged by size:
4513 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2431 2872 2431
Size: 441 5617
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6058
PsiI TTA'TAA
Cuts at: 3628 3628
Size: 6058
PspOMI G'GGCC_C
Cuts at: 3017 3017
Size: 6058
PstI C_TGCA'G
Cuts at: 830 3410 830
Size: 2580 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6058
PvuII CAG'CTG
Cuts at: 2350 2350
Size: 6058
SacII CC_GC'GG
Cuts at: 2608 2608
Size: 6058
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SapI GCTCTTCn'nnn_
Cuts at: 2138 2219 2138
Size: 81 5977
SbfI CC_TGCA'GG
Cuts at: 3410 3410
Size: 6058
ScaI AGT'ACT
Cuts at: 4306 6018 4306
Size: 1712 4346
SexAI A'CCwGG_T
Cuts at: 2868 2868
Size: 6058
SfcI C'TryA_G
Cuts at: 826 1045 3406 4541 5222 5413 826
Size: 219 2361 1135 681 191 1471
Fragments arranged by size:
2361 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6058
SmaI CCC'GGG
Cuts at: 1872 2119 3385 1872
Size: 247 1266 4545
Fragments arranged by size:
4545 1266 247
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4162 5063 5325 820
Size: 197 926 2219 901 262 1553
Fragments arranged by size:
2219 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2847 3849 493
Size: 2354 1002 2702
Fragments arranged by size:
2702 2354 1002
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 3416 1132
Size: 2284 3774
SspI AAT'ATT
Cuts at: 5 52 3982 5
Size: 47 3930 2081
Fragments arranged by size:
3930 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6058
StyI C'CwwG_G
Cuts at: 513 1065 2736 2836 3497 513
Size: 552 1671 100 661 3074
Fragments arranged by size:
3074 1671 661 552 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2544 4085 4102 4255 4440 4885 325
Size: 1255 964 1541 17 153 185 445 1498
Fragments arranged by size:
1541 1498 1255 964 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2376 2772 4304
Size: 274 80 33 51 1443 399 396 1532
Cuts at: 4304 6016 96
Size: 1712 138
Fragments arranged by size:
1712 1532 1443 399 396 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3116 3148 6018 6050 3116
Size: 32 2870 32 3124
Fragments arranged by size:
3124 2870 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2546 2806 2870 1316
Size: 1230 260 64 4504
Fragments arranged by size:
4504 1230 260 64
XbaI T'CTAG_A
Cuts at: 3394 3394
Size: 6058
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6058
XmaI C'CCGG_G
Cuts at: 1870 2117 3383 1870
Size: 247 1266 4545
Fragments arranged by size:
4545 1266 247
XmnI GAAnn'nnTTC
Cuts at: 4187 4187
Size: 6058
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3866 276
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SanDI SfiI SfoI
SrfI SwaI XcmI