Restriction map for
pFN21AB4810
(Circular) (Six-base) MAPSORT of: pFN21AB4810.seq Check: 7896 from: 1 to: 5620
pFN21AB4810 5620 bp
With 182 enzymes: SgfI *
January 17, 2012 16:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2517 2963 1937
Size: 580 446 4594
Fragments arranged by size:
4594 580 446
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2817 3683 5067 5335 1632
Size: 1185 866 1384 268 1917
Fragments arranged by size:
1917 1384 1185 866 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 2525 2675 1166
Size: 1359 150 4111
Fragments arranged by size:
4111 1359 150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5620
AlwNI CAG_nnn'CTG
Cuts at: 2205 3363 4935 2205
Size: 1158 1572 2890
Fragments arranged by size:
2890 1572 1158
ApaLI G'TGCA_C
Cuts at: 3677 4833 3677
Size: 1156 4464
ApoI r'AATT_y
Cuts at: 1493 2236 2551 2837 2929 3155 3307 5416 1493
Size: 743 315 286 92 226 152 2109 1697
Fragments arranged by size:
2109 1697 743 315 286 226 152 92
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2945 1870
Size: 73 1002 4545
Fragments arranged by size:
4545 1002 73
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2941 4396 618
Size: 325 217 627 1154 1455 1842
Fragments arranged by size:
1842 1455 1154 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2406 2939 1391
Size: 1015 533 4072
Fragments arranged by size:
4072 1015 533
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2121 2844 928
Size: 971 222 723 3704
Fragments arranged by size:
3704 971 723 222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 3681 4837 1790
Size: 1891 1156 2573
Fragments arranged by size:
2573 1891 1156
BmgBI CAC'GTC
Cuts at: 1318 2527 1318
Size: 1209 4411
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3351 4309 442
Size: 416 53 83 399 24 1934 958 1753
Fragments arranged by size:
1934 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4280 1239
Size: 3041 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2644 3745 4610 4908 2644
Size: 1101 865 298 3356
Fragments arranged by size:
3356 1101 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4283 882
Size: 617 2784 2219
Fragments arranged by size:
2784 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3411 493
Size: 711 2207 2702
Fragments arranged by size:
2702 2207 711
BsaBI GATnn'nnATC
Cuts at: 2180 5614 2180
Size: 3434 2186
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5395
Fragments arranged by size:
5395 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2030 2362 2816 1186
Size: 841 3 332 454 3990
Fragments arranged by size:
3990 841 454 332 3
BseYI C'CCAG_C
Cuts at: 4823 5319 4823
Size: 496 5124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2450 1163
Size: 573 714 4333
Fragments arranged by size:
4333 714 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2690 3681 3766 4837 2690
Size: 991 85 1071 3473
Fragments arranged by size:
3473 1071 991 85
BsmI GAATG_Cn'
Cuts at: 2128 3131 3224 2128
Size: 1003 93 4524
Fragments arranged by size:
4524 1003 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2373 2373
Size: 5620
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2406 2690 2939 3681 3766 4837 1391
Size: 399 616 284 249 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 616 399 284 249 85
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 3509 5239 3509
Size: 1730 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 844
Size: 2131 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 1394
Size: 429 1684 3507
Fragments arranged by size:
3507 1684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4115 4289 65
Size: 1183 2867 174 1396
Fragments arranged by size:
2867 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2579 4264 4451 1700
Size: 110 769 1685 187 2869
Fragments arranged by size:
2869 1685 769 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 2171 3680 4692 2171
Size: 1509 1012 3099
Fragments arranged by size:
3099 1509 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstXI CCAn_nnnn'nTGG
Cuts at: 2218 2218
Size: 5620
BstYI r'GATC_y
Cuts at: 1972 2070 2202 2950 3317 3702 3719 5171 5257
Size: 98 132 748 367 385 17 1452 86
Cuts at: 5257 5269 5615 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 748 385 367 346 132 98
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2127 2673 513
Size: 552 1062 546 3460
Fragments arranged by size:
3460 1062 552 546
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 2024 2433 3136 3930 3950 2024
Size: 409 703 794 20 3694
Fragments arranged by size:
3694 794 703 409 20
ClaI AT'CG_AT
Cuts at: 1554 2499 1554
Size: 945 4675
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2842 2924 2842
Size: 82 5538
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 3067 3956
Size: 54 1425 50 275 18 231 1006 889
Cuts at: 3956 5585 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1006 889 275 231 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2339 2678 2793 3550 1353
Size: 986 339 115 757 3423
Fragments arranged by size:
3423 986 757 339 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2561 4207 4581 4727 387
Size: 1064 138 972 1646 374 146 1280
Fragments arranged by size:
1646 1280 1064 972 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2817 3683 4280 5067 5335 1239
Size: 393 1185 866 597 787 268 1524
Fragments arranged by size:
1524 1185 866 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2091 2806 2859 2091
Size: 715 53 4852
Fragments arranged by size:
4852 715 53
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 2058 4767 1678
Size: 380 2709 2531
Fragments arranged by size:
2709 2531 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2260 2292 2338 2370 2715
Size: 32 135 32 688 32 46 32 345
Cuts at: 2715 2747 4261 4293 4335 4367 1373
Size: 32 1514 32 42 32 2626
Fragments arranged by size:
2626 1514 688 345 135 46 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2781 748
Size: 2033 3587
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4734 4918 1305
Size: 3429 184 2007
Fragments arranged by size:
3429 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2063 10
Size: 54 1425 343 231 3567
Fragments arranged by size:
3567 1425 343 231 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2216 2361 2525 2561 2675 3578
Size: 648 1005 45 145 164 36 114 903
Cuts at: 3578 3937 4096 518
Size: 359 159 2042
Fragments arranged by size:
2042 1005 903 648 359 164 159 145
114 45 36
MspA1I CmG'CkG
Cuts at: 1606 2538 3036 3713 4861 5106 1606
Size: 932 498 677 1148 245 2120
Fragments arranged by size:
2120 1148 932 677 498 245
NaeI GCC'GGC
Cuts at: 2581 2581
Size: 5620
NcoI C'CATG_G
Cuts at: 513 1065 2127 513
Size: 552 1062 4006
Fragments arranged by size:
4006 1062 552
NdeI CA'TA_TG
Cuts at: 387 2701 387
Size: 2314 3306
NgoMIV G'CCGG_C
Cuts at: 2579 2579
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2978 4523 1132
Size: 97 1749 1545 2229
Fragments arranged by size:
2229 1749 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5620
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2091 2091
Size: 5620
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2588 2972 830
Size: 1758 384 3478
Fragments arranged by size:
3478 1758 384
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SapI GCTCTTCn'nnn_
Cuts at: 2678 2678
Size: 5620
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2584 2846 2968 4103 4784 4975 826
Size: 219 1539 262 122 1135 681 191 1471
Fragments arranged by size:
1539 1471 1135 681 262 219 191 122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2623 3724 4625 4887 820
Size: 197 926 680 1101 901 262 1553
Fragments arranged by size:
1553 1101 926 901 680 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 2606 1517
Size: 1089 4531
StyI C'CwwG_G
Cuts at: 513 1065 2127 3059 513
Size: 552 1062 932 3074
Fragments arranged by size:
3074 1062 932 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2555 3647 3664 3817 4002 4447 325
Size: 1255 975 1092 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1092 975 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3866 5578 96
Size: 274 80 33 51 1443 1889 1712 138
Fragments arranged by size:
1889 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5580 5612 5580
Size: 32 5588
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI