Restriction map for
pFN21AB4802
(Circular) (Six-base) MAPSORT of: pFN21AB4802.seq Check: 7054 from: 1 to: 8221
pFN21AB4802 8221 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4495 5522 4495
Size: 1027 7194
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6031 278
Size: 53 83 186 936 4495 2468
Fragments arranged by size:
4495 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3805 5564 1937
Size: 1868 1759 4594
Fragments arranged by size:
4594 1868 1759
Acc65I G'GTAC_C
Cuts at: 5542 5542
Size: 8221
AclI AA'CG_TT
Cuts at: 1563 6348 6721 1563
Size: 4785 373 3063
Fragments arranged by size:
4785 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4122 6284 7668 7936 1632
Size: 2490 2162 1384 268 1917
Fragments arranged by size:
2490 2162 1917 1384 268
AfeI AGC'GCT
Cuts at: 2408 2879 3230 3541 4610 2408
Size: 471 351 311 1069 6019
Fragments arranged by size:
6019 1069 471 351 311
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8024
AflIII A'CryG_T
Cuts at: 1203 2975 7120 8097 1203
Size: 1772 4145 977 1327
Fragments arranged by size:
4145 1772 1327 977
AgeI A'CCGG_T
Cuts at: 5274 7052 5274
Size: 1778 6443
AhdI GACnn_n'nnGTC
Cuts at: 6950 6950
Size: 8221
AleI CACnn'nnGTG
Cuts at: 1166 2819 3294 1166
Size: 1653 475 6093
Fragments arranged by size:
6093 1653 475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2874 4887 1184
Size: 1690 2013 4518
Fragments arranged by size:
4518 2013 1690
AlwNI CAG_nnn'CTG
Cuts at: 2449 2530 3793 3842 3997 4784 5068 5964 7536
Size: 81 1263 49 155 787 284 896 1572
Cuts at: 7536 2449
Size: 3134
Fragments arranged by size:
3134 1572 1263 896 787 284 155 81
49
ApaI G_GGCC'C
Cuts at: 2382 3635 3690 2382
Size: 1253 55 6913
Fragments arranged by size:
6913 1253 55
ApaLI G'TGCA_C
Cuts at: 2883 6278 7434 2883
Size: 3395 1156 3670
Fragments arranged by size:
3670 3395 1156
ApoI r'AATT_y
Cuts at: 1493 2625 5530 5756 5908 8017 1493
Size: 1132 2905 226 152 2109 1697
Fragments arranged by size:
2905 2109 1697 1132 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6775 160
Size: 872 5743 1606
Fragments arranged by size:
5743 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8221
AvaI C'yCGr_G
Cuts at: 1870 1943 2478 3106 3634 5546 1870
Size: 73 535 628 528 1912 4545
Fragments arranged by size:
4545 1912 628 535 528 73
BamHI G'GATC_C
Cuts at: 3873 5316 5551 5918 3873
Size: 1443 235 367 6176
Fragments arranged by size:
6176 1443 367 235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2520 3102 4286 5012 5201
Size: 325 217 627 733 582 1184 726 189
Cuts at: 5201 5309 5542 6997 618
Size: 108 233 1455 1842
Fragments arranged by size:
1842 1455 1184 733 726 627 582 325
233 217 189 108
BanII G_rGCy'C
Cuts at: 1391 2382 3175 3635 3690 4924 5142 5540 1391
Size: 991 793 460 55 1234 218 398 4072
Fragments arranged by size:
4072 1234 991 793 460 398 218 55
BbeI G_GCGC'C
Cuts at: 3106 3106
Size: 8221
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4320 5336 928
Size: 971 2421 1016 3813
Fragments arranged by size:
3813 2421 1016 971
BbvCI CC'TCA_GC
Cuts at: 3470 4896 4902 5052 3470
Size: 1426 6 150 6639
Fragments arranged by size:
6639 1426 150 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2498 2532 4562 4596 4700 4734 6412
Size: 34 561 34 2030 34 104 34 1678
Cuts at: 6412 6446 1903
Size: 34 3678
Fragments arranged by size:
3678 2030 1678 561 104 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6113 7323 6113
Size: 1210 7011
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8221
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2539 3675 3711
Size: 107 122 71 631 605 867 1136 36
Cuts at: 3711 4484 4754 6832 136
Size: 773 270 2078 1525
Fragments arranged by size:
2078 1525 1136 867 773 631 605 270
122 107 71 36
BglII A'GATC_T
Cuts at: 8216 8216
Size: 8221
BlpI GC'TnA_GC
Cuts at: 4157 4191 4512 5434 5638 4157
Size: 34 321 922 204 6740
Fragments arranged by size:
6740 922 321 204 34
Bme1580I G_kGCm'C
Cuts at: 1790 2382 2688 2887 2967 3506 3635 3690 4606
Size: 592 306 199 80 539 129 55 916
Cuts at: 4606 4882 5227 5314 6282 7438 1790
Size: 276 345 87 968 1156 2573
Fragments arranged by size:
2573 1156 968 916 592 539 345 306
276 199 129 87 80 55
BmgBI CAC'GTC
Cuts at: 1318 3583 1318
Size: 2265 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2170 3179 5952
Size: 416 53 83 399 24 753 1009 2773
Cuts at: 5952 6910 442
Size: 958 1753
Fragments arranged by size:
2773 1753 1009 958 753 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8221
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 3932 2428
Size: 1504 6717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2200 2929 3869 3974 4037 4070 4340 4360
Size: 961 729 940 105 63 33 270 20
Cuts at: 4360 4526 5474 6881 1239
Size: 166 948 1407 2579
Fragments arranged by size:
2579 1407 961 948 940 729 270 166
105 63 33 20
Bpu10I CC'TnA_GC
Cuts at: 3470 3951 4229 4896 4902 4995 5052 3470
Size: 481 278 667 6 93 57 6639
Fragments arranged by size:
6639 667 481 278 93 57 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2674 6346 7211 7509 2674
Size: 3672 865 298 3386
Fragments arranged by size:
3672 3386 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2002 2156 2905 3119 4038 4082 4204
Size: 617 503 154 749 214 919 44 122
Cuts at: 4204 4610 5351 6884 882
Size: 406 741 1533 2219
Fragments arranged by size:
2219 1533 919 749 741 617 503 406
214 154 122 44
BsaAI yAC'GTr
Cuts at: 493 1204 6012 493
Size: 711 4808 2702
Fragments arranged by size:
4808 2702 711
BsaBI GATnn'nnATC
Cuts at: 8215 8215
Size: 8221
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2931 3103 6028 6410
Size: 53 83 186 936 1398 172 2925 382
Cuts at: 6410 7045 275
Size: 635 1451
Fragments arranged by size:
2925 1451 1398 936 635 382 186 172
83 53
BsaWI w'CCGG_w
Cuts at: 3093 3151 3567 4149 5274 6654 7052 7326 7473
Size: 58 416 582 1125 1380 398 274 147
Cuts at: 7473 7884 3093
Size: 411 3430
Fragments arranged by size:
3430 1380 1125 582 416 411 398 274
147 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2450 2480 4013 4016 4043
Size: 30 165 30 1047 30 1533 3 27
Cuts at: 4043 4046 1178
Size: 3 5353
Fragments arranged by size:
5353 1533 1047 165 30 30 30 27
3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2401 3356 4034 4037 4290 4293 4296 5150
Size: 1215 955 678 3 253 3 3 854
Cuts at: 5150 5357 1186
Size: 207 4050
Fragments arranged by size:
4050 1215 955 854 678 253 207 3
3 3
BseYI C'CCAG_C
Cuts at: 2708 2730 4536 4605 5110 5184 5296 7424 7920
Size: 22 1806 69 505 74 112 2128 496
Cuts at: 7920 2708
Size: 3009
Fragments arranged by size:
3009 2128 1806 505 496 112 74 69
22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2155 2446 2843 3068 3239 3556 1163
Size: 573 419 291 397 225 171 317 5828
Fragments arranged by size:
5828 573 419 397 317 291 225 171
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5671 6432 7460 1540
Size: 275 186 3670 761 1028 2301
Fragments arranged by size:
3670 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2887 3403 4163 5142 5502 6282 6367 7438 2887
Size: 516 760 979 360 780 85 1071 3670
Fragments arranged by size:
3670 1071 979 780 760 516 360 85
BsmI GAATG_Cn'
Cuts at: 2945 5732 5825 2945
Size: 2787 93 5341
Fragments arranged by size:
5341 2787 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3573 4019 3573
Size: 446 7775
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2382 2688 2887 2967 3175 3403 3506
Size: 399 592 306 199 80 208 228 103
Cuts at: 3506 3635 3690 4163 4606 4882 4924 5142 5227
Size: 129 55 473 443 276 42 218 85
Cuts at: 5227 5314 5502 5540 6282 6367 7438 1391
Size: 87 188 38 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 592 473 443 399 306
276 228 218 208 199 188 129 103
87 85 85 80 55 42 38
BspEI T'CCGG_A
Cuts at: 3093 3151 4149 7884 3093
Size: 58 998 3735 3430
Fragments arranged by size:
3735 3430 998 58
BspHI T'CATG_A
Cuts at: 2361 6110 7840 2361
Size: 3749 1730 2742
Fragments arranged by size:
3749 2742 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2109 2815 3137 4186 4495 4675 4971 5053
Size: 1265 706 322 1049 309 180 296 82
Cuts at: 5053 5522 5576 844
Size: 469 54 3489
Fragments arranged by size:
3489 1265 1049 706 469 322 309 296
180 82 54
BsrBI CCG'CTC
Cuts at: 1394 1823 3073 4638 5022 6108 1394
Size: 429 1250 1565 384 1086 3507
Fragments arranged by size:
3507 1565 1250 1086 429 384
BsrDI GCAATG_nn'
Cuts at: 65 1248 2965 3820 4815 4869 6716 6890 65
Size: 1183 1717 855 995 54 1847 174 1396
Fragments arranged by size:
1847 1717 1396 1183 995 855 174 54
BsrFI r'CCGG_y
Cuts at: 1700 1810 2390 2861 2901 2914 3099 4289 4478
Size: 110 580 471 40 13 185 1190 189
Cuts at: 4478 4884 5274 6865 7052 1700
Size: 406 390 1591 187 2869
Fragments arranged by size:
2869 1591 1190 580 471 406 390 189
187 185 110 40 13
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8221
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8221
BssSI C'ACGA_G
Cuts at: 6281 7293 6281
Size: 1012 7209
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3138 3793 5068 5222 794
Size: 2344 655 1275 154 3793
Fragments arranged by size:
3793 2344 1275 655 154
BstBI TT'CG_AA
Cuts at: 7025 7025
Size: 8221
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8221
BstXI CCAn_nnnn'nTGG
Cuts at: 3376 4306 4384 4433 5110 5489 3376
Size: 930 78 49 677 379 6108
Fragments arranged by size:
6108 930 677 379 78 49
BstYI r'GATC_y
Cuts at: 1972 3873 3903 4006 4806 5316 5472 5551 5918
Size: 1901 30 103 800 510 156 79 367
Cuts at: 5918 6303 6320 7772 7858 7870 8216 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1901 1452 800 510 385 367 346
156 103 86 79 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 3977 4313 3977
Size: 336 7885
BtgI C'CryG_G
Cuts at: 513 1065 2071 3856 5361 513
Size: 552 1006 1785 1505 3373
Fragments arranged by size:
3373 1785 1505 1006 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7482
Fragments arranged by size:
7482 683 56
BtsI GCAGTG_nn'
Cuts at: 2856 3168 3203 3506 4132 4935 4982 5737 6531
Size: 312 35 303 626 803 47 755 794
Cuts at: 6531 6551 2856
Size: 20 4526
Fragments arranged by size:
4526 803 794 755 626 312 303 47
35 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8221
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4348 4383 529
Size: 35 3784 35 4367
Fragments arranged by size:
4367 3784 35 35
DraI TTT'AAA
Cuts at: 5525 5525
Size: 8221
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7228 809
Size: 507 5912 1802
Fragments arranged by size:
5912 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2764 3420 3427
Size: 54 1425 50 275 18 934 656 7
Cuts at: 3427 3585 3957 5072 5668 6557 8186 8
Size: 158 372 1115 596 889 1629 43
Fragments arranged by size:
1629 1425 1115 934 889 656 596 372
275 158 54 50 43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 5668 1537
Size: 275 3856 4090
Fragments arranged by size:
4090 3856 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4094 6151 1353
Size: 2741 2057 3423
Fragments arranged by size:
3423 2741 2057
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2474 2933 3542 5132 6808 7182
Size: 1064 138 885 459 609 1590 1676 374
Cuts at: 7182 7328 387
Size: 146 1280
Fragments arranged by size:
1676 1590 1280 1064 885 609 459 374
146 138
EcoICRI GAG'CTC
Cuts at: 5140 5140
Size: 8221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2200 2929 3869 3974 4037 4070 4122
Size: 393 568 729 940 105 63 33 52
Cuts at: 4122 4340 4360 4526 5474 6284 6881 7668 7936
Size: 218 20 166 948 810 597 787 268
Cuts at: 7936 1239
Size: 1524
Fragments arranged by size:
1524 948 940 810 787 729 597 568
393 268 218 166 105 63 52 33
20
EcoNI CCTnn'n_nnAGG
Cuts at: 4019 4967 4019
Size: 948 7273
EcoO109I rG'GnC_Cy
Cuts at: 2076 2098 2115 2378 2796 2807 3315 3380 3631
Size: 22 17 263 418 11 508 65 251
Cuts at: 3631 3687 3790 3839 3994 4485 2076
Size: 56 103 49 155 491 5812
Fragments arranged by size:
5812 508 491 418 263 251 155 103
65 56 49 22 17 11
EcoRI G'AATT_C
Cuts at: 5530 5908 5530
Size: 378 7843
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3268 3268
Size: 8221
FspI TGC'GCA
Cuts at: 2782 6727 2782
Size: 3945 4276
HaeII r_GCGC'y
Cuts at: 1678 2410 2715 2881 3106 3232 3355 3543 4612
Size: 732 305 166 225 126 123 188 1069
Cuts at: 4612 4632 7368 1678
Size: 20 2736 2531
Fragments arranged by size:
2736 2531 1069 732 305 225 188 166
126 123 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2450 2482 2579 2611 3635
Size: 32 135 32 878 32 97 32 1024
Cuts at: 3635 3667 4016 4048 6862 6894 6936 6968 1373
Size: 32 349 32 2814 32 42 32 2626
Fragments arranged by size:
2814 2626 1024 878 349 135 97 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5565 5811 669
Size: 988 281 3627 246 3079
Fragments arranged by size:
3627 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4066 4301 748
Size: 3318 235 4668
Fragments arranged by size:
4668 3318 235
HpaI GTT'AAC
Cuts at: 5811 5811
Size: 8221
KasI G'GCGC_C
Cuts at: 3102 3102
Size: 8221
KpnI G_GTAC'C
Cuts at: 5546 5546
Size: 8221
MfeI C'AATT_G
Cuts at: 3037 5820 3037
Size: 2783 5438
MluI A'CGCG_T
Cuts at: 8097 8097
Size: 8221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2596 4227 5376 7335 7519 1305
Size: 1291 1631 1149 1959 184 2007
Fragments arranged by size:
2007 1959 1631 1291 1149 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3422 3429 3587 3959 10
Size: 54 1425 343 1590 7 158 372 4272
Fragments arranged by size:
4272 1590 1425 372 343 158 54 7
MslI CAynn'nnrTG
Cuts at: 518 1166 2766 2819 3294 5366 5487 6179 6538
Size: 648 1600 53 475 2072 121 692 359
Cuts at: 6538 6697 518
Size: 159 2042
Fragments arranged by size:
2072 2042 1600 692 648 475 359 159
121 53
MspA1I CmG'CkG
Cuts at: 1606 2326 2819 2993 3082 3294 3660 3693 4003
Size: 720 493 174 89 212 366 33 310
Cuts at: 4003 4455 4519 4546 5110 5438 5637 6314 7462
Size: 452 64 27 564 328 199 677 1148
Cuts at: 7462 7707 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 720 677 564 493 452 366
328 310 245 212 199 174 89 64
33 27
NaeI GCC'GGC
Cuts at: 2392 2863 2392
Size: 471 7750
NarI GG'CG_CC
Cuts at: 3103 3103
Size: 8221
NcoI C'CATG_G
Cuts at: 513 1065 5361 513
Size: 552 4296 3373
Fragments arranged by size:
4296 3373 552
NdeI CA'TA_TG
Cuts at: 387 3019 387
Size: 2632 5589
NgoMIV G'CCGG_C
Cuts at: 2390 2861 2390
Size: 471 7750
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8221
NotI GC'GGCC_GC
Cuts at: 5668 5668
Size: 8221
NspI r_CATG'y
Cuts at: 1132 1229 2054 2398 2979 3218 3331 5579 7124
Size: 97 825 344 581 239 113 2248 1545
Cuts at: 7124 1132
Size: 2229
Fragments arranged by size:
2248 2229 1545 825 581 344 239 113
97
PasI CC'CwG_GG
Cuts at: 1348 4473 1348
Size: 3125 5096
PciI A'CATG_T
Cuts at: 2975 7120 2975
Size: 4145 4076
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2093 2283 2449 3831 4612 4930 1108
Size: 985 190 166 1382 781 318 4399
Fragments arranged by size:
4399 1382 985 781 318 190 166
PfoI T'CCnGG_A
Cuts at: 3317 3780 3869 4689 4731 5037 3317
Size: 463 89 820 42 306 6501
Fragments arranged by size:
6501 820 463 306 89 42
PmeI GTTT'AAAC
Cuts at: 5525 5525
Size: 8221
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2300 2332 6290 6322 7803 7835 2300
Size: 32 3958 32 1481 32 2686
Fragments arranged by size:
3958 2686 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2098 2115 3315 3380 2098
Size: 17 1200 65 6939
Fragments arranged by size:
6939 1200 65 17
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8221
PsiI TTA'TAA
Cuts at: 5791 5791
Size: 8221
PspOMI G'GGCC_C
Cuts at: 2378 3631 3686 2378
Size: 1253 55 6913
Fragments arranged by size:
6913 1253 55
PstI C_TGCA'G
Cuts at: 830 2325 2745 2806 3547 4002 4460 4794 4989
Size: 1495 420 61 741 455 458 334 195
Cuts at: 4989 5573 830
Size: 584 3478
Fragments arranged by size:
3478 1495 741 584 458 455 420 334
195 61
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8221
PvuII CAG'CTG
Cuts at: 2326 3082 4003 4519 5110 2326
Size: 756 921 516 591 5437
Fragments arranged by size:
5437 921 756 591 516
SacI G_AGCT'C
Cuts at: 5142 5142
Size: 8221
SalI G'TCGA_C
Cuts at: 1936 5563 1936
Size: 3627 4594
SanDI GG'GwC_CC
Cuts at: 3315 3380 3315
Size: 65 8156
SbfI CC_TGCA'GG
Cuts at: 5573 5573
Size: 8221
ScaI AGT'ACT
Cuts at: 6469 8181 6469
Size: 1712 6509
SfcI C'TryA_G
Cuts at: 826 1045 2236 2321 2741 2802 3543 3998 4456
Size: 219 1191 85 420 61 741 455 458
Cuts at: 4456 4790 4985 5569 6704 7385 7576 826
Size: 334 195 584 1135 681 191 1471
Fragments arranged by size:
1471 1191 1135 741 681 584 458 455
420 334 219 195 191 85 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 4484 4754 4484
Size: 270 7951
SfoI GGC'GCC
Cuts at: 3104 3104
Size: 8221
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8221
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8221
SmaI CCC'GGG
Cuts at: 1872 3108 3636 5548 1872
Size: 1236 528 1912 4545
Fragments arranged by size:
4545 1912 1236 528
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2653 6325 7226 7488 820
Size: 197 926 710 3672 901 262 1553
Fragments arranged by size:
3672 1553 926 901 710 262 197
SnaBI TAC'GTA
Cuts at: 493 6012 493
Size: 5519 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8221
SphI G_CATG'C
Cuts at: 1132 2054 2398 5579 1132
Size: 922 344 3181 3774
Fragments arranged by size:
3774 3181 922 344
SrfI GCCC'GGGC
Cuts at: 3636 3636
Size: 8221
SspI AAT'ATT
Cuts at: 5 52 6145 5
Size: 47 6093 2081
Fragments arranged by size:
6093 2081 47
StuI AGG'CCT
Cuts at: 1517 2542 1517
Size: 1025 7196
StyI C'CwwG_G
Cuts at: 513 1065 5361 5660 513
Size: 552 4296 299 3074
Fragments arranged by size:
4296 3074 552 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 2800 4363 5102 5288 6248 6265
Size: 1255 609 611 1563 739 186 960 17
Cuts at: 6265 6418 6603 7048 325
Size: 153 185 445 1498
Fragments arranged by size:
1563 1498 1255 960 739 611 609 445
186 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6467 8179 96
Size: 274 80 33 51 1443 4490 1712 138
Fragments arranged by size:
4490 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2504 2536 3317 3349 5308 5340 8181 8213 2504
Size: 32 781 32 1959 32 2841 32 2512
Fragments arranged by size:
2841 2512 1959 781 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4030 1316
Size: 2714 5507
XbaI T'CTAG_A
Cuts at: 5557 5557
Size: 8221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3017 4840 5368 3017
Size: 1823 528 5870
Fragments arranged by size:
5870 1823 528
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8221
XmaI C'CCGG_G
Cuts at: 1870 3106 3634 5546 1870
Size: 1236 528 1912 4545
Fragments arranged by size:
4545 1912 1236 528
XmnI GAAnn'nnTTC
Cuts at: 6350 6350
Size: 8221
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6029 276
Size: 53 83 186 936 4495 2468
Fragments arranged by size:
4495 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SapI SexAI SwaI